Öz
Büyük hacimdaki hücrelerin uzun süreli muhafazası için -130·c
nin altındaki sıcaklıklara ihtiyaç vardır. -196.C'ye dayanıklı hücre
dondurma ampullerinin maksimum kapasitesi 5 ml'dir. 100 ml hacimdaki hücrelerin dondurularak muhafazası için ticarette sadece palikarbonat ampuller bulunmaktadır ki bunlar da ancak -150·c·ye kadar bozulmadan kalabilmektedirler. Bu nedenle denemeler sıvı azot gazında hücre muhafazası konusunda yoğunlaştırılmıştır.
Bu çalışmada 15 seri hücre kültürü 100 ml hacimda dondurulmuştur.
Bu hücrelerin çözdürüldükten sonraki üreyebilirlik oranının
ortalama % 90 olduğu tespit edilmiştir.
Anahtar Kelimeler
Hücre Kültürü Hücre Kültürünün Stoklanması S. İsmet GÜRHAN Aysun ÖZTÜRKMEN Tamer ERGÜÇ 1990 Etlik Veteriner Mikrobiyoloji Dergisi
Kaynakça
- CORIELL, .L. (1979) : Preservation, Storage and Shipment. Methods in Enzymology, Vol. LVIII, eds. William B. Jakoby and Ira H. Pastan, Acd. Press.
- DOYLE, A., MORRIS, C.B., ARMITA:GE, W.J. : Cryopreservation of Animal Cells Upstream Processes: Equipment and Techniques, pp. 1-7, 1888 Alan R. liss, lnç.
- GRIFFITHS, J.B. Cell Biology : Experimental -Aspects, Animal Cell Biotechnology,eds. Ra/ E. Spier and J.B. Griffiths, vol. 1, ·1335 Acad Prsss.
- JENSEN, M . (1975) : Storage of Large Volumes of Calls. Report of the Meeting of the Research Group of the Stand. Tech. Comm. Eur. Comm. Cont. FMD. Brescia./Padua, Sep. 1975. . .
- KASTEN, F..H. and : YIP; D.K., : A Simple Device and Procedure for Succesful Freezing of Cells in Liquid Nitrogen Vapor. Methods in. Cell Bliology, ed. David M. PresGott, vol. XIV, 1976 Acad Press.
- MAREL, P., :Concentration. Animal •Cell Biotechnology Vol. .2. eds. Ray E.Spier and J .B. Griffiths, 18.S5 Acad. Press. · .
- SHARP, R.J. (1984) : The preservation of Genetically Unstable Microorganisms and the Cryopreservation of Firmentation Seed Cultures in Advances in Biotechnological Proceses 3; eds . Alan R. Liss: Inc., 81-109 ., New York.
Öz
For long term storage of so large quantities of cells it, ls necessary
to keep them below minus 130·c. The maximum capacity
of the ampoules which are not demaged in minus 196·c is limited
with 5 ml' To freeze the cells in at least 100 ml volume only polycarbonate ampaules are commercially available. Those are resistant to steam sterilisation and freezing untill minus 156·c. For that reason the experiment is realised in the gas phase of liquid nitrogen.
Kaynakça
- CORIELL, .L. (1979) : Preservation, Storage and Shipment. Methods in Enzymology, Vol. LVIII, eds. William B. Jakoby and Ira H. Pastan, Acd. Press.
- DOYLE, A., MORRIS, C.B., ARMITA:GE, W.J. : Cryopreservation of Animal Cells Upstream Processes: Equipment and Techniques, pp. 1-7, 1888 Alan R. liss, lnç.
- GRIFFITHS, J.B. Cell Biology : Experimental -Aspects, Animal Cell Biotechnology,eds. Ra/ E. Spier and J.B. Griffiths, vol. 1, ·1335 Acad Prsss.
- JENSEN, M . (1975) : Storage of Large Volumes of Calls. Report of the Meeting of the Research Group of the Stand. Tech. Comm. Eur. Comm. Cont. FMD. Brescia./Padua, Sep. 1975. . .
- KASTEN, F..H. and : YIP; D.K., : A Simple Device and Procedure for Succesful Freezing of Cells in Liquid Nitrogen Vapor. Methods in. Cell Bliology, ed. David M. PresGott, vol. XIV, 1976 Acad Press.
- MAREL, P., :Concentration. Animal •Cell Biotechnology Vol. .2. eds. Ray E.Spier and J .B. Griffiths, 18.S5 Acad. Press. · .
- SHARP, R.J. (1984) : The preservation of Genetically Unstable Microorganisms and the Cryopreservation of Firmentation Seed Cultures in Advances in Biotechnological Proceses 3; eds . Alan R. Liss: Inc., 81-109 ., New York.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Veteriner Cerrahi |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Aralık 1990 |
| Gönderilme Tarihi | 1 Aralık 1990 |
| Yayımlandığı Sayı | Yıl 1990 Cilt: 6 Sayı: 6 |