«Studies on isolation of Haemophilus somnus from bovine diseased and normal pulmonary tissues»

Yıl 1992, Cilt: 7 Sayı: 2, 49 - 62, 01.12.1992

Vildan Özdemir

Öz

Haemophilus somnus was isolated and identified from four lungs of
130 lungs with pneumonia and from one of 90 lungs with normal appearance.
The collected materials were seeded on to blood agar, Mac Conkey
and brain-heart infusion agar with blood and yeast extract using steril
swabs. The organisms grew well only on brain-heart infusion agar with blood
and yeast extract in the presence of % 1 O C02 atmesphere and after 48
hours incubation. The colonies were greyish yellow in colour, translucent,
rounded, 1 mm in diamater and butter-like in consistency. The isolated organisms
were gram negative, pleomophic and coccobacilli and they fermented
glucose. lndol and oxidase tests were positive but catalase negative.
All the isolates tested with H.somnus antiserum and gave agglutination
in the slide-agglutination test.

Normal ve Pnöymonili Sığır Akciğerlerinden Haemophilus Somnus İzolasyonu Üzerinde Çalışmalar

Öz

130 Pnöymonili akciğerden dört ve 90 normal görünüşlü sığır akciğerinden
1 adet Haemophilus somnus izole ve idenlifiye edildi. Toplanan materyallerden
steril eküvyonlarla kanlı agar, Mac Conkey agar ve kanlı - maya
özetli beyin-kalp infüzyon agara ekimler yapıldı. Sadece kanlı-maya özetli
beyin-kalp infüzyon agarda% 10 CO2 atmosferde, 48 saat inkubasyondan
·sonra üreme belirlendi. Koloniler; grimsi sarı renkli, tereyağ kıvamında, 1
mm çaplı, yarı saydam, yuvarlak şekilli olarak görüldüler. Izole edilen gram
negatif, pleomorfik, küçük kokobasil şeklindeki mikroorganizmalar glukozu
fermente ettiler. lndol ve oksidaz pozitif, katalaz negatif bulundu. H.somnus
antiserumu ile kontrol edilen izolatların tümü lamda aglutinasyon verdi.

Anahtar Kelimeler

Sığır, Sığır Akciğerlerinden Haemophilus Somnus İsolazyonu, Haemophilus Somnus İsolazyonu, 1992, Vildan ÖZDEMİR, Etlik Veteriner Mikrobiyoloji Dergisi

Kaynakça

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