Araştırma Makalesi
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Taze, Sulandırılmış ve Dondurulmuş Boğa Spermasının Çeşitli Mikroorganizmlar ve Mikoplazma Yönünden İncelenmesi

Yıl 1992, Cilt: 7 Sayı: 2, 117 - 125, 01.12.1992

Öz

Bu çalışmada Tarım, Orman ve Köyleri Bakanlığı, istanbul il Müdürlüğü'ne
bağlı Şenlikköy Sun'i Tohumlama Istasyonu'nda sperma vericisi olarak
kullanılan ondört adet boğanın sperması, gram(+), gram(-) mikroorganizmalar
ve mikaplazma yönünden incelenmiştir. Boğaların spermaları
taze, sulandırılmış ( Fransa'dan ithal edilen bir sulandırıcı ile) ve payet halinde
dondurulmuş şekillerde mikrobiyolojik muayene için ayrı ayrı ekilmiştir.
Taze spermada yapılan ekimler sonucunda 5 boğada proteus spp.
ve Aspergilus fumigatus üremiş, sulandırılmış spermada ise 6 boğadan
E.coli, E.frondi ve Aspergilus fumigatus izole edilmiştir. Sulandırma sıvısında
ise, E.coli, diplokok, streptekok ve maya tespit edilmiştir. Incelenen
sperma numunelerinde mikoplazma izole edilememiştir.

Kaynakça

  • ALEEM, M., CHAUDHRY, R.A., NAEEM, U.K., RIZVI, A.U.R., AHMAD, R. (1990) : Occurence of pathogenic bacetira in buffalo semen. Buffalo J. 6(1) 93-98.
  • BEŞE, M. (1974) : Mikrobiyolojide kullanılan biyokimyasal testler ve besiyerleri, A. ü.Vet. Fak. Yayınları 298. 208-209.
  • BEŞE, M. (1974) : Mikrobiyolojide kullanılan biyokimyasal testler ve besiyerleri. A. ü.Vet. Fak. Yayınları 298. 397-398.
  • BORYCZKO, Z., KAHN, W., VELEZ, G. (1985) : Effect of bacteria in bull semen on the motility of spermatozoa and on their ATP and ADP content. Zuchthygiene 20(5). (abs).
  • ERDAG, 0 ., TÜRKASLAN, J. (1989) : Pendik Hayvan Hast. Merk. Araşt. Ens. Dergisi. XIX (1-2) 85-97.
  • FEJES, J. (1986) : Pathological changes in the genital system of breeding bulls with mycoplasmas in semen. Vet. bulletin. 7234. (abs).
  • FISH, N.A., ROSENDAL, S., MILLER, R.B. (1985) : The distribution of mycoplasmas and ureaplasmas in the genital tract of normal antificial insemination bulls. Can. Vet. J. 26. 13-15.
  • GANGADHAR, K.S., RAV, R.V., KRISHNASWAMY, S., RAO S.U. (1986): Bacterial and fungal types and the ir Icad in the frozen semen of buffalo bu lls. lndian Vet. J. 63 (1) 48-53.
  • GOURLAY, R.N . (1973) : Significance of mycoplasma infections in cattle. J.A.V.M.A. 163. 905-909.
  • GÖZÜ, M. (1983) : Boyama yöntemleri. Pendik Vet. Kent. ve Araş. Ens. Yayınları . No. 7. 243-246.
  • HAMDY, A.H ., MILLER, C.C. (1971) : Antibiyotics for bovine myocoplasma. J. Dairy Sci. 54. 1541 -1544.
  • HOTZEL, H., SACHSE, K. , PFUTZNER, H. (1990) : DNA probesfor the decettion of mycoplasma bovis and Mycoplasmagenitalium. IOM. letters. vol. 1. 384-385.
  • LAFAUNCE, N.A., MCENTEE, K. (1982) : Experimental Mycoplasma bovis seminal vesiculitis in the bull. The Comeli Vet. 72.150-167.
  • LANGFORD, E. V. (1951) : Mycoplasma species recovered from the reproductive tracts of Western Canadian Cows. Can. J. Comp. Med. 39 133-138.
  • MARINOV. P., BOHNEL, H. (1974) : Hygienic conditions of materials for freezing bovine semen. j. Dairy Sc. 57: 6.707-711 .
  • NATALIA, L. (1986) : Yeast flora of bull smen. Vet. Bulletin. 4995. (abs).
  • NIMAI, S., SINHA, A.K., BALRAJ, S., SINGH, L.C., TIWARY, D.K., SINGH, M.P. (.1990): Efficacy of specific antibiotics on microbial load chilled and frozen semen of crossbred bulls. Vet. Bulletin. 2355 (abs).
  • ONOVIRAN, 0 ., TRRUSCOTT, R.B., FISH, N.A., BARKER, C., RUHNKE, H.L. (1975) : · The recovery of mycoplasmas from genital tracts of bulls in artifivial breeding units in Ontario. J. Comp.Med. 39. 474-475.
  • PAREZ, M., GUERIN, B. (1983) : Sanitary and hygienic procedures for controlling ubiquitous bacteira. Laboratuire pour le centrole des reproducteures. France. 43-49.
  • PARSONSON, I.M., HAL, C.E., SETTERGREN 1. (1971) : A metod for the çelleetion of bovine seminal vesicle seciretions for microbiologic examination. J.A.V.M.A. 158(2) 175-177.
  • ROSENBUSCH, R. (1990) : Mycoplasmas as agents of b·ovine disease. IOM letters. Vol. 1178-179.
  • SAED, O.M., AL-AUBAIDI, J.M. (1983) : lnfertility in heifers caused by pathogenic strain of mycoplasma bovigenitalium. Comeli Vet. 73. 125-130.
  • SAIKA, G.K., BORO, B.R., HAZARIKA, A.K., AHMED, K. (1987) : Bacterial flora of bovine senıen and the ir antibiotic sensivity paıtern . lndian Vet. Med. J. 11(1) 37-39.
  • STALHEIM, O.H.V. (1983) : Standardized microbiologicaltests for the international exchange at animal genetic material. National Animal Disease Center. lowa. 1-4.
  • STOYANOV, T. (1985 : Reproducüive analysis of microbial centemination in samples of seminal fluid from breeding bu lls. Vet. Bulletin. 1101. (abs).
  • THOMAS, C.B., WOLFGRAM, L.J ., SHARP, P. (1990) : Bovine Neutrophil Chemiluminescence is suressed by Mycoplasma bovis. IOM leıters . Vol. 1. 341-342.
  • TRUSCOTT, R.B., RUHNKE, H.L. (1983) : Control of Mycoplasma and Ureaplasma in semen. Atlantic Area Laboratory, Agriculture Canada. 50-63.
  • UNGUREANI, C., GRIGORE, C., IONITA, I.F., CONSTANTINESCU. (1986) : Frequency of mycoplasmae in the semen of reproduction bulls. Arehiv Für Experimentelle Veterinarmedizin. 40(1) (abs).
  • ZAUGG, N.L., ALMQUIST, J.O. (1973) : Mobility of spermatozoa and control of bacteria in bovine semen containing penicillin and neomycin or epicillin. J.Dairy sci. 56: 202-206.

«A study on the bull semen that is fresh, diluted and frozen, about various microorganisms and mycoplasmas»

Yıl 1992, Cilt: 7 Sayı: 2, 117 - 125, 01.12.1992

Öz

The semen samples collected from fourteen bulls in an Artificial lnsemination
Center in Türkiye were investigated for gram(+) and gram(-) microorganisms
and mycoplasma. Semen samples were evaluated as fresh,
diluted (with an extender imported France) and frozen in 0,25 ml PVC
straws.
Proteus spp. and Aspergilus spp. were observed in semen samples
from five bulls when fresh semen was studied. E.coli, E.frondi and Aspergilus
spp. were isolated in diluted semen. E.coli, Diplococ, Streptecoc and
yeast were seen in diluting extender. No mycoplasma was found in the
samples.

Kaynakça

  • ALEEM, M., CHAUDHRY, R.A., NAEEM, U.K., RIZVI, A.U.R., AHMAD, R. (1990) : Occurence of pathogenic bacetira in buffalo semen. Buffalo J. 6(1) 93-98.
  • BEŞE, M. (1974) : Mikrobiyolojide kullanılan biyokimyasal testler ve besiyerleri, A. ü.Vet. Fak. Yayınları 298. 208-209.
  • BEŞE, M. (1974) : Mikrobiyolojide kullanılan biyokimyasal testler ve besiyerleri. A. ü.Vet. Fak. Yayınları 298. 397-398.
  • BORYCZKO, Z., KAHN, W., VELEZ, G. (1985) : Effect of bacteria in bull semen on the motility of spermatozoa and on their ATP and ADP content. Zuchthygiene 20(5). (abs).
  • ERDAG, 0 ., TÜRKASLAN, J. (1989) : Pendik Hayvan Hast. Merk. Araşt. Ens. Dergisi. XIX (1-2) 85-97.
  • FEJES, J. (1986) : Pathological changes in the genital system of breeding bulls with mycoplasmas in semen. Vet. bulletin. 7234. (abs).
  • FISH, N.A., ROSENDAL, S., MILLER, R.B. (1985) : The distribution of mycoplasmas and ureaplasmas in the genital tract of normal antificial insemination bulls. Can. Vet. J. 26. 13-15.
  • GANGADHAR, K.S., RAV, R.V., KRISHNASWAMY, S., RAO S.U. (1986): Bacterial and fungal types and the ir Icad in the frozen semen of buffalo bu lls. lndian Vet. J. 63 (1) 48-53.
  • GOURLAY, R.N . (1973) : Significance of mycoplasma infections in cattle. J.A.V.M.A. 163. 905-909.
  • GÖZÜ, M. (1983) : Boyama yöntemleri. Pendik Vet. Kent. ve Araş. Ens. Yayınları . No. 7. 243-246.
  • HAMDY, A.H ., MILLER, C.C. (1971) : Antibiyotics for bovine myocoplasma. J. Dairy Sci. 54. 1541 -1544.
  • HOTZEL, H., SACHSE, K. , PFUTZNER, H. (1990) : DNA probesfor the decettion of mycoplasma bovis and Mycoplasmagenitalium. IOM. letters. vol. 1. 384-385.
  • LAFAUNCE, N.A., MCENTEE, K. (1982) : Experimental Mycoplasma bovis seminal vesiculitis in the bull. The Comeli Vet. 72.150-167.
  • LANGFORD, E. V. (1951) : Mycoplasma species recovered from the reproductive tracts of Western Canadian Cows. Can. J. Comp. Med. 39 133-138.
  • MARINOV. P., BOHNEL, H. (1974) : Hygienic conditions of materials for freezing bovine semen. j. Dairy Sc. 57: 6.707-711 .
  • NATALIA, L. (1986) : Yeast flora of bull smen. Vet. Bulletin. 4995. (abs).
  • NIMAI, S., SINHA, A.K., BALRAJ, S., SINGH, L.C., TIWARY, D.K., SINGH, M.P. (.1990): Efficacy of specific antibiotics on microbial load chilled and frozen semen of crossbred bulls. Vet. Bulletin. 2355 (abs).
  • ONOVIRAN, 0 ., TRRUSCOTT, R.B., FISH, N.A., BARKER, C., RUHNKE, H.L. (1975) : · The recovery of mycoplasmas from genital tracts of bulls in artifivial breeding units in Ontario. J. Comp.Med. 39. 474-475.
  • PAREZ, M., GUERIN, B. (1983) : Sanitary and hygienic procedures for controlling ubiquitous bacteira. Laboratuire pour le centrole des reproducteures. France. 43-49.
  • PARSONSON, I.M., HAL, C.E., SETTERGREN 1. (1971) : A metod for the çelleetion of bovine seminal vesicle seciretions for microbiologic examination. J.A.V.M.A. 158(2) 175-177.
  • ROSENBUSCH, R. (1990) : Mycoplasmas as agents of b·ovine disease. IOM letters. Vol. 1178-179.
  • SAED, O.M., AL-AUBAIDI, J.M. (1983) : lnfertility in heifers caused by pathogenic strain of mycoplasma bovigenitalium. Comeli Vet. 73. 125-130.
  • SAIKA, G.K., BORO, B.R., HAZARIKA, A.K., AHMED, K. (1987) : Bacterial flora of bovine senıen and the ir antibiotic sensivity paıtern . lndian Vet. Med. J. 11(1) 37-39.
  • STALHEIM, O.H.V. (1983) : Standardized microbiologicaltests for the international exchange at animal genetic material. National Animal Disease Center. lowa. 1-4.
  • STOYANOV, T. (1985 : Reproducüive analysis of microbial centemination in samples of seminal fluid from breeding bu lls. Vet. Bulletin. 1101. (abs).
  • THOMAS, C.B., WOLFGRAM, L.J ., SHARP, P. (1990) : Bovine Neutrophil Chemiluminescence is suressed by Mycoplasma bovis. IOM leıters . Vol. 1. 341-342.
  • TRUSCOTT, R.B., RUHNKE, H.L. (1983) : Control of Mycoplasma and Ureaplasma in semen. Atlantic Area Laboratory, Agriculture Canada. 50-63.
  • UNGUREANI, C., GRIGORE, C., IONITA, I.F., CONSTANTINESCU. (1986) : Frequency of mycoplasmae in the semen of reproduction bulls. Arehiv Für Experimentelle Veterinarmedizin. 40(1) (abs).
  • ZAUGG, N.L., ALMQUIST, J.O. (1973) : Mobility of spermatozoa and control of bacteria in bovine semen containing penicillin and neomycin or epicillin. J.Dairy sci. 56: 202-206.
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Cerrahi
Bölüm Araştırma Makaleleri
Yazarlar

H. Özge Güney Bu kişi benim

Jale Türkaslan Bu kişi benim

Fijen Güney Bu kişi benim

Yayımlanma Tarihi 1 Aralık 1992
Gönderilme Tarihi 1 Aralık 1992
Yayımlandığı Sayı Yıl 1992 Cilt: 7 Sayı: 2

Kaynak Göster

APA Güney, H. Ö., Türkaslan, J., & Güney, F. (1992). Taze, Sulandırılmış ve Dondurulmuş Boğa Spermasının Çeşitli Mikroorganizmlar ve Mikoplazma Yönünden İncelenmesi. Etlik Veteriner Mikrobiyoloji Dergisi, 7(2), 117-125.


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