Araştırma Makalesi
BibTex RIS Kaynak Göster

Determination of the antibody levels of the cattle immunised against FMD by NT and ELISA

Yıl 1994, Cilt: 7 Sayı: 5, 99 - 108, 01.12.1994

Öz

Simple, cheep and reproducible serological procedures are required
to determine foot and mouth disease (FMD) immune response of the vaccinated
cattle in the field. The antibody levels of the blood sera of the vaccinated
cattle in the field hava been meaured by both microneutralisation
(MN) and liquid phase sandwich enzyme linked immunosorbant assay (ELISA)
techniques which were developed for that purpose, comperatively.
4978 vaccinated cattle blood sera have been received from 41 provinces in
Turkey and tested. As a result, the true prevalance of the immunity among
the vaccinated cattle was estimated as 81,65% against A22 and 76,15%
against o, types. In comparison of the two tesıs he sensitivity of ELISA in
measuring the antibodies which positively effect the immunity have been
found more than MN test.

Kaynakça

  • ABU-ELZEIN, E.M.E. and COUWTHER, J.R. :The specific detection of FMDV whole antifen by ELISA. J. Hyg. Camb., 83, 127-134. 1979.
  • ARIAS, C.A.L., HANSON, R.P. and GERARDINO, A.G. : Antibody response of tropical tests. lnt. Symp. FMD, Lyon, 1976. Dev. Stand., 35, 345-356 (S. Karger, Basel197).
  • BAILEY, N.T.J. : Statistical Mathods in Biology, See. Ed., Edward Arnoldof Hadder and Stoughton, London, 1981.
  • DUBOURGET, P., DTERAZ, N., GARGON, P. and LOMBARD, M. : Serological and biochemical properties of FMD strains recently isolated in Near and Meadle-East. 1. Int. FMD. Symp., June, 1989. Ongun Kard. Mat., Ankara, 1990.
  • DOEL, T.R. : The immuna response of cattle to FMD antigens. Pitman-Moore FMD Bulletin, 29, 1, 1-5. 1991.
  • FEDIDA, M., DANNACHER, G., COUDERT, M., PEILLON, M. and LUCAM, F. : Evelution et duree oe l'immunite antiaphteuse post-vaccinale chez les bovins pluri-vaccines. Bull. Off. Int. Epizoot. 77, 1005-027. 1972.
  • HAMBLIN, C., BARNETI, I.T.R., CROWTER, J.R. : Anew ELI SA for detection of antibodies against FMDV. 1. Development and method of ELISA. J. Imm. Me.th. 93, 115-121.1986.
  • HAMBLIN, C., KITCHING, R.P., OONALOSON, A.l., CROWTER, J.R. and BARNETI, T.R. : ELISA for the detection of antibodies against FMDV. Epidem. Inf., 99, 733-744.1987.
  • LOMBARO, M. : Utulisation de serums de bovins vaccinesdans l'evaluation des souches virales aphteuses du terrain. Sess. Res. Gr. Stand. Tech. Comm. Eur: Comm. Cent. FMO, Lindholm, Oenmark, FAO, Rome, 1979.
  • LOMBARD, M. und PETERMAN, H.G. : Korrelation zwischen der ELISA-Test und der Serumneutralisation zur Titrierung MKS-Virus-neutralisierender antikörper im Serum von Kontrollrindern. Tierarzt. Umschau, 37. 354-356. 1982
  • MACKOWIAK, C., LANG C., FONTAINE, J., CAMANO, R., PETERMAN, H.G. : Relation between neutralising antibody titre and protection in animals immunized against FMD. Ann. lnst. Pasteur, 103, 252-257. 1962.
  • MCCULLOGH, K.C., ACKERMANN, M., MÜLLER, H.K., BRUCKNER, L. and KIHM, U. : Potaney testing of FMD vaccines by challenge and antibody measurement. 1. Int. FMD Symp., June, 1989. Ongun Kard. Mat., Ankara. 1989.
  • OUCHTERLONY, O. : Oiffusion in gels methodsfor immunological analyses. Prog. n Allergy, 5, 1-78. ed. by p. Kallas, Karger-Basel, 1958
  • PANINA, G. and DeSIMONE, F. : Risposta anticorpale dei bovini adiverse dosidi vaccino antiaphtoso. Confronto ıra siero-neutralizzazione ed inibizione della fissazione del complenıento. Atti Della Societa haliana Della Scienze Veterinaire, 25, 518-520. 1971.
  • PAY, T.W.F. and HNIGLEY, P.J. : Corralation of 140S antigen dese with serumneutralizing antibody response and the level of protection induced in callle by FMD vaccines. Vaccine, 5, 60-64. 1987.
  • RIVENSO, S. and CONSTANTINI, H.L.: Aplicacion de la tecnica de Marucci en la fiebre aftosa. Rev.lnvest. Canad., 11,7-16. 1961 .
  • VIZCAINO, S.J.M. and ALVAREZ, C.M. : Enzyme ınınıunoassay techniques, ELISA, in animal and Planı Disease. Off. Int. Ep. Tehnical Series, No. 7, 1987. Paris.
  • WAGNER, G.G. and McVICAR, J.W. : FMD antibodies. Comparison of a tissue culture micro-neutralisation test with the assay in suckling mi.ce. Appl Microb. 20, 995-997. 1970.
  • WESTBURY, H.A., OOUGHTY, W.J., FORMAN, A.J., TANGCHAITRONG, S. ad KONGTHON, A. : A coparison of ELISA, CF and virus isolation for FMD diagnosis. Vet. Mic., 17, 21-28. 1988.
  • WITIMAN, G.: Control of FMD vaccine anda field trial in Federal Rep. of Germany. 1. Int. FMD Symp., June, 1989. Ongun Kard. Mat., Ankara.

Şap Aşılı Sığırlarda Mikronötralizasyon Ve ELİSA İle Antikor Düzeylerinin Saptanması

Yıl 1994, Cilt: 7 Sayı: 5, 99 - 108, 01.12.1994

Öz

Şap hastalığına karşı sahadaki aşılı sığırlarda oluşan immun yanıtı
tesbit etmek için uygulaması kolay, ucuz, aynı zamanda çok fazla materyal
ile çalışmaya elverişli serolajik teknikiere ihtiyaç vardır. Bu amaçla geliştirilmiş
olan mikronötralizasyon ve liquid phase sandwich ELISA (enzyme-
linked immunosorbant assay) ile karşılaştırmalı olarak sahada aşılı
sığırlardan alınan kan serumlarının antikor düzeyleri ölçüldü. 41 ilde şap
hastalığına karşı aşılı sığırlardan alınan toplam 4978 adet sığır kan serumu
numunesi test edildi. Veriler istatistik yöntemler ile değerlendirildi. Sonuçta
bağışıklığın gerçek prevalansı A22 tipi için% 81,65, 0 1 tipi için% 76,15 olarak
tesbit edilirken iki metot arasında yapılan karşılaştırmada ELISA'nın antikor
pozitif serumları tanımlamada seronötralizasyon teste oranla daha
hassas olduğu görüldü.

Kaynakça

  • ABU-ELZEIN, E.M.E. and COUWTHER, J.R. :The specific detection of FMDV whole antifen by ELISA. J. Hyg. Camb., 83, 127-134. 1979.
  • ARIAS, C.A.L., HANSON, R.P. and GERARDINO, A.G. : Antibody response of tropical tests. lnt. Symp. FMD, Lyon, 1976. Dev. Stand., 35, 345-356 (S. Karger, Basel197).
  • BAILEY, N.T.J. : Statistical Mathods in Biology, See. Ed., Edward Arnoldof Hadder and Stoughton, London, 1981.
  • DUBOURGET, P., DTERAZ, N., GARGON, P. and LOMBARD, M. : Serological and biochemical properties of FMD strains recently isolated in Near and Meadle-East. 1. Int. FMD. Symp., June, 1989. Ongun Kard. Mat., Ankara, 1990.
  • DOEL, T.R. : The immuna response of cattle to FMD antigens. Pitman-Moore FMD Bulletin, 29, 1, 1-5. 1991.
  • FEDIDA, M., DANNACHER, G., COUDERT, M., PEILLON, M. and LUCAM, F. : Evelution et duree oe l'immunite antiaphteuse post-vaccinale chez les bovins pluri-vaccines. Bull. Off. Int. Epizoot. 77, 1005-027. 1972.
  • HAMBLIN, C., BARNETI, I.T.R., CROWTER, J.R. : Anew ELI SA for detection of antibodies against FMDV. 1. Development and method of ELISA. J. Imm. Me.th. 93, 115-121.1986.
  • HAMBLIN, C., KITCHING, R.P., OONALOSON, A.l., CROWTER, J.R. and BARNETI, T.R. : ELISA for the detection of antibodies against FMDV. Epidem. Inf., 99, 733-744.1987.
  • LOMBARO, M. : Utulisation de serums de bovins vaccinesdans l'evaluation des souches virales aphteuses du terrain. Sess. Res. Gr. Stand. Tech. Comm. Eur: Comm. Cent. FMO, Lindholm, Oenmark, FAO, Rome, 1979.
  • LOMBARD, M. und PETERMAN, H.G. : Korrelation zwischen der ELISA-Test und der Serumneutralisation zur Titrierung MKS-Virus-neutralisierender antikörper im Serum von Kontrollrindern. Tierarzt. Umschau, 37. 354-356. 1982
  • MACKOWIAK, C., LANG C., FONTAINE, J., CAMANO, R., PETERMAN, H.G. : Relation between neutralising antibody titre and protection in animals immunized against FMD. Ann. lnst. Pasteur, 103, 252-257. 1962.
  • MCCULLOGH, K.C., ACKERMANN, M., MÜLLER, H.K., BRUCKNER, L. and KIHM, U. : Potaney testing of FMD vaccines by challenge and antibody measurement. 1. Int. FMD Symp., June, 1989. Ongun Kard. Mat., Ankara. 1989.
  • OUCHTERLONY, O. : Oiffusion in gels methodsfor immunological analyses. Prog. n Allergy, 5, 1-78. ed. by p. Kallas, Karger-Basel, 1958
  • PANINA, G. and DeSIMONE, F. : Risposta anticorpale dei bovini adiverse dosidi vaccino antiaphtoso. Confronto ıra siero-neutralizzazione ed inibizione della fissazione del complenıento. Atti Della Societa haliana Della Scienze Veterinaire, 25, 518-520. 1971.
  • PAY, T.W.F. and HNIGLEY, P.J. : Corralation of 140S antigen dese with serumneutralizing antibody response and the level of protection induced in callle by FMD vaccines. Vaccine, 5, 60-64. 1987.
  • RIVENSO, S. and CONSTANTINI, H.L.: Aplicacion de la tecnica de Marucci en la fiebre aftosa. Rev.lnvest. Canad., 11,7-16. 1961 .
  • VIZCAINO, S.J.M. and ALVAREZ, C.M. : Enzyme ınınıunoassay techniques, ELISA, in animal and Planı Disease. Off. Int. Ep. Tehnical Series, No. 7, 1987. Paris.
  • WAGNER, G.G. and McVICAR, J.W. : FMD antibodies. Comparison of a tissue culture micro-neutralisation test with the assay in suckling mi.ce. Appl Microb. 20, 995-997. 1970.
  • WESTBURY, H.A., OOUGHTY, W.J., FORMAN, A.J., TANGCHAITRONG, S. ad KONGTHON, A. : A coparison of ELISA, CF and virus isolation for FMD diagnosis. Vet. Mic., 17, 21-28. 1988.
  • WITIMAN, G.: Control of FMD vaccine anda field trial in Federal Rep. of Germany. 1. Int. FMD Symp., June, 1989. Ongun Kard. Mat., Ankara.
Toplam 20 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Bilimleri
Bölüm Araştırma Makaleleri
Yazarlar

Burhan Gürhan Bu kişi benim

Erdoğan Şenel Bu kişi benim

Gülhan Dakılır Bu kişi benim

Hasan Öztürkmen Bu kişi benim

Yayımlanma Tarihi 1 Aralık 1994
Gönderilme Tarihi 1 Aralık 1994
Yayımlandığı Sayı Yıl 1994 Cilt: 7 Sayı: 5

Kaynak Göster

APA Gürhan, B., Şenel, E., Dakılır, G., Öztürkmen, H. (1994). Şap Aşılı Sığırlarda Mikronötralizasyon Ve ELİSA İle Antikor Düzeylerinin Saptanması. Etlik Veteriner Mikrobiyoloji Dergisi, 7(5), 99-108.


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