Kanatlı Hayvanlarda Mycoplasma Gallisepticum ve Mycoplasma Synoviae'ya Karşı Oluşan Antikorların Tesbitinde Serum Lam Aglutinasyon (SPA), Hemaglutinasyon-İnhibisyon (HI) ve İmmunocomb Katı-Faz İmmuoassay Yöntemlerinin Karşılaştırılması

Yıl 1996, Cilt: 8 Sayı: 3, 33 - 45, 01.12.1996

Ömer M. Esendal Hakan Yardımcı Nejat Aydın

Öz

Bu çalışmada, kanatlı hayvanlarda Mycoplasma infeksiyonlanna karşı oluşan
spesifik antikorlarm ortaya konulmasında rutin olarak kullanılan SPA ve HI
testlerinin yanısıra, enzim immunoassay prensibine dayanan ImmunoComb
test kitinin karşılaştırmalı olarak denenınesi ve rutin uygulamadaki öneminin ortaya
konulması amaçlanmıştır. Denemede kullamlan 70 adet tavuk serumu MG
antikorlan yönünden SPA testi ile incelendiğinde 26 serumun (%37.14) pozitif,
44'ünün ise (%62.86) negatif olduğu saptanmıştır. öte yandan, MS antikorları
yönünden 21 serumun (%30) pozitif, 49 serumun ise (%70) negatif olduğu
görülmüştür. Aynı serumların HI testi ile incelenmesinde MG yönünden 15
adedinin (%21. 43) pozitif ve 55 adedinin (% 78 .57) negatif olduğu; MS yönünden
ise 23 seium (o/o3 2. 8 6) pozitif ve 4 7 serum (%6 7. 14) negatif olduğu saptanmışıtr.
MG yönünden lC ile incelenen serumlarm 20 adedinin (%28.57) pozitif ve 50
adedinin (%71.43) negatif sonuç verdiği ; MS yönünden ise 25 adet serumun
(%35 .71) pozitif ve 45 adet serumun da (%64.29) negatif sonuç verdiği
belirlenmiştir. Her 3 testin karşılaştınlmasında pozitiflik ve negatif}ik yönünden
paralellik bulunduğu gözlenmiş özellikle, m ile lC arasında bu paralleliğin çok daha dikkat çekici olduğu ve IC'un biraz daha duyarlı çalıştığı ortaya konmuştur.
Bu nedenle, IC test kitüıin pratikte uygulanabilir, duyarlı bir test olarak diğer
konvansiyonel testlerle birlikte güvenle kullanılabileceği kanısına varılmıştır.

Anahtar Kelimeler

MS, Kanatlı, Mycoplasma Gallisepticum, MG, Mycoplasma Synoviae, Serum Lam Aglutinasyon, SPA, Hemaglutinasyon-İnhibisyon, HI, ImmunoComb, ImmunoComb Katı-Faz İmmunoassay, Ömer M. ESENDAL, Hakan YARDIMCI, Nejat AYDIN

Comparisom Of Serum Plate Agglutination (SPA) Haemagglutination-Inhibition (HI) and Immunocomb Solid-Phase Immunoassay Methods In THE Detection Of Antibodies Produced In Chickens Against Mycoplasma Gallisepticum and Mycoplasma Synoviae

Öz

In this study, the use, effıciency and reliability of ImmunoComb test kit,
which works according to the enzyrne irnmunoassay principle,together with
other conventionally used serological tests, such as SPA and lll, were investigatedin the detection of specific antibodies produced against Mycoplasma infectionsin poultry. SPA test results of70 field sera revealed that, 26 (37.14%)
were positive and 49 (70%) were negative for MS antibodies. In the HI test, 15
(21.43%) samples were found positive and 55 (78.57%) were negative for
MG, and 23 (32.86%) samples were found positive and 47 (67.14%) were
negative for MS, antibodies. On the other hand, IC test results showed that, 20
(28.57%) samples were positive and 50 (71.43%) samples were negative for
MG, and 25 (35 .71%) were positive and 45 (67.29%) were negative for MS
antibodies. The comparison of these 3 tests indicated that, a good correlation
existed among them, particularly, a more pronounced one between HI and IC
tests, in the detection of specific antibodies, and IC test was slightly mo re sen.
sitive than the other two. Therefore, it was concluded that, the IC test kit could
be used conveniently, together with other conventional serelogical tests in the
detection of specific antibodies produced against Mycoplasma infections in
poultry.

Kaynakça

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