Bu çalışmada yumurta tavuklarında yaş ve verimin serum Gama-GT,
GüT,GPT ve CK düzeyleri üzerine etkil~ri incelenmiştir. Bu amaçla 50 adet
. yumurtacı civciv kullanılmıştır. Hisex Brown hibrit civcivlerinden 3 günlükten
yumurta veriminde pike ulaşınca ya dek kan serumu alınınışı tr. Hayvanlar ı 7.
haftada yumurtlamaya başlamışlar ve yumurta verimi %94.3 ile 29. haftada
pike ulaşmıştır. Gama-GT düzeyi 7., 10. ve 27. haftalarda yükselmiş, diğer
haftalarda ise önemli farklılıklar meydana gelmemiştir. İlk dört haftada yüksek
olan GüT düzeyi 7. haftada düşmüş, enzimin düzeyinde 7, 1O ve 12. haftalarda
önemli bir değişiklik olmamıştır. Yumurta üretimine geçiş öncesi olan 15. haftada
yükselme, 19. haftada düşme meydana gelmiştir. Bundan sonraki haftalarda
yavaş yavaş artarak yumurta veriminde pike ulaşılan 29. haftada önemli bir
yükselme meydana gelmiştir. GPT düzeyinde 12. ve 25 . haftalardaki düşüş dışında önemli bir farklılık görülmemiştir. CK düzeyinde 12. haftada önemli bir düşme,10, 15 , 19, 25 . haftalarda yükselme meydana gelmiştir.
Arlian, L.G., Ahmed, M. and Vyszenski-Moher, D.L. (1988): Effects of
S. Scabie var. canis (Acari: Sarcoptidae) on blood indexes ofparasitized rabbits.
J.Med. Entomol., 25 :360-369 (Abstr.)
Balachandran, C. and Ramarkrishnan, R. (ı988): Influence of dietary
aflatoxin on certain serum enzyme levels in broiler chickens. Mycopathologia,
101:65-67. (Abstr.)
Bayşu , N. (ı979) : Temel Biyokimya. Fırat Univ. Vet. Fak. Yayın No : ı8 .
Beljan, J.R., Madley, T.I., Hellewel, A.B. and Hein, L.J. (1970): netermination
of selected avian blood plasma chernistry values us ing the Technicon
Aı:itoAnalyzer. Poult. Sci., 50:2290232.
Bogin, E., Ratner, D. and Avidar, Y. (1983): Biochemica1 changes in
b1ood and tissues associated whit Raund Heart Disease in Turkey poults. Avian
Pathol., 12:437-442.
Boorman, K.N., and Lewis, D. (1971): Protein metaboli.sm. In: Bell,
. D.J., Freeman, B.M.; Phisyology and Biochemistry of the Domestic Fowl. Academic
Press Ine., pp.339-366.
Bowes, V.A, Julian, RJ. and Stirtzinger, T. (19SY): Comparisyon of
serum biochemical profiles of male broilers with famele broilers and White
Leghom chickens. Can. J. Vet. Res., 53: 7-11.
Brake, J. and Thaxton, P. (1979): Physiological changes in caged layers
during a forced mo lt. 1.Body temperature and selected blood constituents. Poult.
Sci., 58:699-706.
Broun,J.p., Bezille, P., Galtier, P., Rico, AG., Ouderaogo, G. (1992):
Effects of age on the distribution of same enzyıns in organ of sheep. Small
Rum. Res., 9:149-156.
Chatter jee, M., Ghosh, S.P., Chakraborty, AK., Desarkar, M.k., Chatter
jee, S. (1979): A note on the effect of starvation and sex hormones on blood
glucose and gluconesogenesis in male and female goats (Capra hircus
bengalesnsis). Indian J. Anim. Sci., 49:677-679. (Abstr.)
Çamaş, H, Ertürk, K.ve Ersoy, E. (1976): Normal ve musculaer
dystrophie' li kuzulann kan serumlannda total protein, protein fraksiyonlan,
kreatinin, kreatinfosfokinaz, Glutamik-Piruvik-Transaminaz ve GlutamikOkzalasetik-
Transaminaz yönünden araştırmalar. Ankara Üniv.Vet.Fak.Derg.,
. 23: 249-259.
Deshpande, P.M., Chatter Jee, AK. and Datta, I. C. (1976): Biochemical
changes in the blood and egg formation. Indian J.Anim. Sci., 46:36-38.
Ersoy, E., Bayşu, N., Ertürk, K. ve üstadal, K.M. (1979): Biyokimya.
Ankara Üniv.Vet. Fak. Yayın No:358.
Fishman, W.H. (1960): Plasma enzyınes . In: Putnam, F.W.: The Plazma
Proteins. Academic Press. Vol.II, pp. 59-97.
Georgie, G.C. , Chand, D. and Razdan, M.N. (1973): seasonal changes
in palsına cholesterol and serum alkaline phosphatase and transaminases activities
in cross- bred cattle. Indian J. Exp. Biol. 11 :448-450. (Abstr.)
Gildersleeve, RP., Satterlee, D.G., Jonson, W.A. and Scott, T.R. (1983):
The effects Of forced molt treatment on blood biochemicals in hens. Poult.
Sci., 62:755-762.
Hollands, K.G., Grunder, AA., Williams, C.J. and Gavora, J.S . (1980):
Plasma kreatine kinase as an indicator of degenerative myopathy in live Turkeys.
Br. Poult. Sci., 21:161-169.
Johson, R.J., Choice, A., Farrell, D.J. and Cumming, R.B. (1984): Production
respanses of layer strain hens to food restriction during rearing. Br.
Poult. Sci., 25:369-387.
Kamal, T.H., Habebe, AA., Abdel-Samee, AM., and Marai, I.F. {1989):
Milk production ofheat-stressed Friesian cows and its improvement in the subtropics.
Europian Associ. Anim. Product. Netherlands, 38:156-158.
King, J. (1965: Practical Clinical Biochemistry. Camalot Press. Ltd.
Suothampton, pp. 139-147.
Kirchhoff, A. (1993 ): Commparative patthological histoological and elinical
studies on stress myopathy in differant breeds of fowl, with difference to
the pathogenesis of subracoracoid muscle necrosis in male broilers. Inaguaral
Dissertation, Fachbereich Veterinarimedizin, Berlin (Abstr.).
Kumar, P., Kumar, M., Joshi, H.C {1993): Serum enzyıns profile in
experimental hypomagnezemia in yeaarling buffaloes. Indian J.Anim. Sci., 63:
1152-1155.
Mahzunlar, H. {1990): Klinikman normal ve deneysei olarak hypothyroidi
oluşturulan köpeklerde, EKG parametreleri ve SGOT, SGPT, LDH, CPK enzim
düzeyleri üzerinde karşılaştırmalı araştırmalar. İstanbul Üniv. Sağlık Bilimleri
Enstitüsü Doktora Tezi. İstanbul.
Malherbe. WD., Kellerman, T.S., Kriek, N.P.J. andHaupt, W.H. (1977):
Garrıma-glutamyl transpeptidase activity in speep serum: Normal valuesandan
evaluation of its potential for detecting li ver involvement in experimental lupinosis.
Onderstepoort J. Vet. Res., 44:29-38.
Matsuzava, T., Noomura, M., Unno, T. (1993): Clunucal pathology reference
ranges of laboratory animals. J. Vet. Med. S ci., 55:351-362.
McDaniel, L.S . and Chute, H.L. (ı 96 ı): Enzyrne activity levels in ebieken
ranges oflaboratory animals. J. Vet. Med. Sci., 55:351-362
McDaniel, L.S. and Dempsey, H.A. (1963): The effects offasting ufon
plasma anzyme levels in chickens. 3.Sixth International Congress on Nutrition,
Edinburg, Scotland.
Mengi, A. ( 1979): Biyolojik ve stres faktörlerinin sıçan karaciğer! erindeki
gamaglutarniltranspepti dase (y-GT) aktivitesine tesiri. VI. Bilim Kongresi
,Veteriner ve Hayvancılık Araştırma Grubu Tebliğleri, s. 287-292.
Mengi, A. (1980): Gama-glutamil transpeptidaz (y-GT). İstanbul üniv.
Vet. Fak. Derg., 6:95-102.
Mengi, A. (1991) Biyokimya. İstanbul üniv. Vet. Fak. Yayın. No: 12.
Mengi, A. ve Serpek, B. (1981): Normal, Fasciolasis'li ve Hydatidosis'li
koyunlarda serum gamaglutarniltranspeptidaz (y-GT) aktivitesinin saptanması.
İstanbul Üniv. Vet. Fak. Derg., 7:13-17.
Mengi, A., Serpek, B. ve Bilal, T. (1983): Normal ve hasta köpeklerin
kan serumlannda glütamik oksalasetik transaminaz (GOT), glütarnik pirüvik
transaminaz ve gama glutamil transpeptidaz aktiviteleri ile serüloplazmin
konsantrasyonu üzerinde çalışmalar. İstanbul üniv. Vet. Fak. Derg , 9:21-27.
Obe, D.C.B. and Studdert, V.P. (1990): Bailliere's Comprehensive
Vetrarinary Dictionary. Second Edition, Bailliere Tindall, London.
Pons, A., Garcia, F.J., Palou, A. and Alemany, M. (1986): Effects of
starvation anda protein diet on the amino acid matabolism enzyme activities of
the organs ofdomestic fowl hatchlings. Comp. Biochem. Physiol, 85B (1):275-
278.
Rivetz, B. and Bogin, E. (1982): Enzyınatic changes in serum and tissues
in fowl infected with a neurotropicmesogenic strain ofNewcastle Disease
Virus. AvanPathol., 11:407-425.
Simesen, M.G., Nielsen, K. and Nasnsen, P.(l973): Some effects of
experimental Fasciola hepatica infection in cattle on the serum activities of yglutamly
transpeptidase and glutarnic oxaloacetic transarninase. Res. Vet. Sci.,
15:32-36. .
S pano, J.S., Pedersoli, W.M., Kemppainen, RJ., Krist L.M. and Young,
D.W. (1987): Baseline hematologic, endokrine, and elinical chemistıy valuesin
ducks and roosters. Avian Disease, 31 :800-803.
Sreemannarayana, 0., Marguardt, R.R., Frohlich, AA. and Guenter,
W. (1989): Enzyıne ativities, protein, metabolites and electrolyte concentra.tions
in the serum of Single Comb White Leghorn chickens. Indian Vet. J., 66:435-
440.
Pener, S.(l988): Etanot'ün kobayda karaciğer ve serum gammaglutamyl-
transferase (GGT) aktivitesi üzerine etkisi. İstanbul Üniv. Vet. Fak.
Derg. 14:1-10.
Tietz, N.W. (1987): Fundamental of Clinical Chemistry. Third Edition.
W.B. Saunders Company, pp. 353-392.
Töre, İ.R. (1978): Enzim testleri ve veteriner kliniğinde uygulanmalan.
İstanbul Üniv .. Vet. Fak. Derg., 4:39-62.
Tripp, M.J. and Schmitz, J.A. (1982): Influence ofphysical exercise on
palsına creatinekinase activity in helaty and dystrophic Turkeys and sheep. Am.
J. Vet. Res. 43 :2220-2223.
Türkalp, 1., Aktüre, S., Mengi, A. (1988): Viral hepatitlerde gamma.
glutamil-tarnspeptidaz. Pişli Çocuk Hast. Tıp BQlteni. Ayn Baskı .
Visser, I.J.R., Vermeulen, D.J., Vellema, P.P., Gruys, E. (1992):Total
CK and CK-Bb activity in serum from sheep with scrapie. Vet. Quart., 14:157-
158.
The Effekts Of Age and Egg Produktuon On Serum Gama-GT,GOT,GPT and CK Leves İn Layers
In this study, the effect of age and egg production on serum GOT, GPT,
gama-GT and CK levels were investigated. Commercialy available strains of
50 Hisex Brown chicks were used and sera of animals were collected from 3
· days untill the pik Jevel of egg production. Egg production started at 17. week and piked with 94.3 %at 29. week. No significant differences were found in
gama-GT levels except teh increases at 7 27. weeks. The GOT levels were
high during the first fo ur weeks and decreased at 7. week Although no significant
differences were seen 7., 10. and 12. weeks, atthe begininf of egg production
(at 15. week) the enzyın levels increased and at 19. week it decreased.
Thereafter, GOT levels increased gradually and at 29. week it significantly
increased. No significant differences were found in GPT levels except the
decrease at 12. and 25 weeks. The Ok levels decreased markedly at 12. week
and increased at 10., 15., 19. and 25 . weeks.
Kaynakça
Aktaş ,M. , Auguste, D., Lefevre, H.P., Tautain, P.P., Braun, J.P. (1993):
Creatine kinase in dog: A review. Vet. Res. Comm., 17(5): 353-369.
Anonim (1976) : Sentinel Chernicals Ck-NAC. Milano.
Arlian, L.G., Ahmed, M. and Vyszenski-Moher, D.L. (1988): Effects of
S. Scabie var. canis (Acari: Sarcoptidae) on blood indexes ofparasitized rabbits.
J.Med. Entomol., 25 :360-369 (Abstr.)
Balachandran, C. and Ramarkrishnan, R. (ı988): Influence of dietary
aflatoxin on certain serum enzyme levels in broiler chickens. Mycopathologia,
101:65-67. (Abstr.)
Bayşu , N. (ı979) : Temel Biyokimya. Fırat Univ. Vet. Fak. Yayın No : ı8 .
Beljan, J.R., Madley, T.I., Hellewel, A.B. and Hein, L.J. (1970): netermination
of selected avian blood plasma chernistry values us ing the Technicon
Aı:itoAnalyzer. Poult. Sci., 50:2290232.
Bogin, E., Ratner, D. and Avidar, Y. (1983): Biochemica1 changes in
b1ood and tissues associated whit Raund Heart Disease in Turkey poults. Avian
Pathol., 12:437-442.
Boorman, K.N., and Lewis, D. (1971): Protein metaboli.sm. In: Bell,
. D.J., Freeman, B.M.; Phisyology and Biochemistry of the Domestic Fowl. Academic
Press Ine., pp.339-366.
Bowes, V.A, Julian, RJ. and Stirtzinger, T. (19SY): Comparisyon of
serum biochemical profiles of male broilers with famele broilers and White
Leghom chickens. Can. J. Vet. Res., 53: 7-11.
Brake, J. and Thaxton, P. (1979): Physiological changes in caged layers
during a forced mo lt. 1.Body temperature and selected blood constituents. Poult.
Sci., 58:699-706.
Broun,J.p., Bezille, P., Galtier, P., Rico, AG., Ouderaogo, G. (1992):
Effects of age on the distribution of same enzyıns in organ of sheep. Small
Rum. Res., 9:149-156.
Chatter jee, M., Ghosh, S.P., Chakraborty, AK., Desarkar, M.k., Chatter
jee, S. (1979): A note on the effect of starvation and sex hormones on blood
glucose and gluconesogenesis in male and female goats (Capra hircus
bengalesnsis). Indian J. Anim. Sci., 49:677-679. (Abstr.)
Çamaş, H, Ertürk, K.ve Ersoy, E. (1976): Normal ve musculaer
dystrophie' li kuzulann kan serumlannda total protein, protein fraksiyonlan,
kreatinin, kreatinfosfokinaz, Glutamik-Piruvik-Transaminaz ve GlutamikOkzalasetik-
Transaminaz yönünden araştırmalar. Ankara Üniv.Vet.Fak.Derg.,
. 23: 249-259.
Deshpande, P.M., Chatter Jee, AK. and Datta, I. C. (1976): Biochemical
changes in the blood and egg formation. Indian J.Anim. Sci., 46:36-38.
Ersoy, E., Bayşu, N., Ertürk, K. ve üstadal, K.M. (1979): Biyokimya.
Ankara Üniv.Vet. Fak. Yayın No:358.
Fishman, W.H. (1960): Plasma enzyınes . In: Putnam, F.W.: The Plazma
Proteins. Academic Press. Vol.II, pp. 59-97.
Georgie, G.C. , Chand, D. and Razdan, M.N. (1973): seasonal changes
in palsına cholesterol and serum alkaline phosphatase and transaminases activities
in cross- bred cattle. Indian J. Exp. Biol. 11 :448-450. (Abstr.)
Gildersleeve, RP., Satterlee, D.G., Jonson, W.A. and Scott, T.R. (1983):
The effects Of forced molt treatment on blood biochemicals in hens. Poult.
Sci., 62:755-762.
Hollands, K.G., Grunder, AA., Williams, C.J. and Gavora, J.S . (1980):
Plasma kreatine kinase as an indicator of degenerative myopathy in live Turkeys.
Br. Poult. Sci., 21:161-169.
Johson, R.J., Choice, A., Farrell, D.J. and Cumming, R.B. (1984): Production
respanses of layer strain hens to food restriction during rearing. Br.
Poult. Sci., 25:369-387.
Kamal, T.H., Habebe, AA., Abdel-Samee, AM., and Marai, I.F. {1989):
Milk production ofheat-stressed Friesian cows and its improvement in the subtropics.
Europian Associ. Anim. Product. Netherlands, 38:156-158.
King, J. (1965: Practical Clinical Biochemistry. Camalot Press. Ltd.
Suothampton, pp. 139-147.
Kirchhoff, A. (1993 ): Commparative patthological histoological and elinical
studies on stress myopathy in differant breeds of fowl, with difference to
the pathogenesis of subracoracoid muscle necrosis in male broilers. Inaguaral
Dissertation, Fachbereich Veterinarimedizin, Berlin (Abstr.).
Kumar, P., Kumar, M., Joshi, H.C {1993): Serum enzyıns profile in
experimental hypomagnezemia in yeaarling buffaloes. Indian J.Anim. Sci., 63:
1152-1155.
Mahzunlar, H. {1990): Klinikman normal ve deneysei olarak hypothyroidi
oluşturulan köpeklerde, EKG parametreleri ve SGOT, SGPT, LDH, CPK enzim
düzeyleri üzerinde karşılaştırmalı araştırmalar. İstanbul Üniv. Sağlık Bilimleri
Enstitüsü Doktora Tezi. İstanbul.
Malherbe. WD., Kellerman, T.S., Kriek, N.P.J. andHaupt, W.H. (1977):
Garrıma-glutamyl transpeptidase activity in speep serum: Normal valuesandan
evaluation of its potential for detecting li ver involvement in experimental lupinosis.
Onderstepoort J. Vet. Res., 44:29-38.
Matsuzava, T., Noomura, M., Unno, T. (1993): Clunucal pathology reference
ranges of laboratory animals. J. Vet. Med. S ci., 55:351-362.
McDaniel, L.S . and Chute, H.L. (ı 96 ı): Enzyrne activity levels in ebieken
ranges oflaboratory animals. J. Vet. Med. Sci., 55:351-362
McDaniel, L.S. and Dempsey, H.A. (1963): The effects offasting ufon
plasma anzyme levels in chickens. 3.Sixth International Congress on Nutrition,
Edinburg, Scotland.
Mengi, A. ( 1979): Biyolojik ve stres faktörlerinin sıçan karaciğer! erindeki
gamaglutarniltranspepti dase (y-GT) aktivitesine tesiri. VI. Bilim Kongresi
,Veteriner ve Hayvancılık Araştırma Grubu Tebliğleri, s. 287-292.
Mengi, A. (1980): Gama-glutamil transpeptidaz (y-GT). İstanbul üniv.
Vet. Fak. Derg., 6:95-102.
Mengi, A. (1991) Biyokimya. İstanbul üniv. Vet. Fak. Yayın. No: 12.
Mengi, A. ve Serpek, B. (1981): Normal, Fasciolasis'li ve Hydatidosis'li
koyunlarda serum gamaglutarniltranspeptidaz (y-GT) aktivitesinin saptanması.
İstanbul Üniv. Vet. Fak. Derg., 7:13-17.
Mengi, A., Serpek, B. ve Bilal, T. (1983): Normal ve hasta köpeklerin
kan serumlannda glütamik oksalasetik transaminaz (GOT), glütarnik pirüvik
transaminaz ve gama glutamil transpeptidaz aktiviteleri ile serüloplazmin
konsantrasyonu üzerinde çalışmalar. İstanbul üniv. Vet. Fak. Derg , 9:21-27.
Obe, D.C.B. and Studdert, V.P. (1990): Bailliere's Comprehensive
Vetrarinary Dictionary. Second Edition, Bailliere Tindall, London.
Pons, A., Garcia, F.J., Palou, A. and Alemany, M. (1986): Effects of
starvation anda protein diet on the amino acid matabolism enzyme activities of
the organs ofdomestic fowl hatchlings. Comp. Biochem. Physiol, 85B (1):275-
278.
Rivetz, B. and Bogin, E. (1982): Enzyınatic changes in serum and tissues
in fowl infected with a neurotropicmesogenic strain ofNewcastle Disease
Virus. AvanPathol., 11:407-425.
Simesen, M.G., Nielsen, K. and Nasnsen, P.(l973): Some effects of
experimental Fasciola hepatica infection in cattle on the serum activities of yglutamly
transpeptidase and glutarnic oxaloacetic transarninase. Res. Vet. Sci.,
15:32-36. .
S pano, J.S., Pedersoli, W.M., Kemppainen, RJ., Krist L.M. and Young,
D.W. (1987): Baseline hematologic, endokrine, and elinical chemistıy valuesin
ducks and roosters. Avian Disease, 31 :800-803.
Sreemannarayana, 0., Marguardt, R.R., Frohlich, AA. and Guenter,
W. (1989): Enzyıne ativities, protein, metabolites and electrolyte concentra.tions
in the serum of Single Comb White Leghorn chickens. Indian Vet. J., 66:435-
440.
Pener, S.(l988): Etanot'ün kobayda karaciğer ve serum gammaglutamyl-
transferase (GGT) aktivitesi üzerine etkisi. İstanbul Üniv. Vet. Fak.
Derg. 14:1-10.
Tietz, N.W. (1987): Fundamental of Clinical Chemistry. Third Edition.
W.B. Saunders Company, pp. 353-392.
Töre, İ.R. (1978): Enzim testleri ve veteriner kliniğinde uygulanmalan.
İstanbul Üniv .. Vet. Fak. Derg., 4:39-62.
Tripp, M.J. and Schmitz, J.A. (1982): Influence ofphysical exercise on
palsına creatinekinase activity in helaty and dystrophic Turkeys and sheep. Am.
J. Vet. Res. 43 :2220-2223.
Türkalp, 1., Aktüre, S., Mengi, A. (1988): Viral hepatitlerde gamma.
glutamil-tarnspeptidaz. Pişli Çocuk Hast. Tıp BQlteni. Ayn Baskı .
Visser, I.J.R., Vermeulen, D.J., Vellema, P.P., Gruys, E. (1992):Total
CK and CK-Bb activity in serum from sheep with scrapie. Vet. Quart., 14:157-
158.
Uyanık, F., Önal, A. A., Işcan, K. M., Sevüktekin, S. (1996). Yumurta Tavuklarında Yaş ve Verimin Serum Gama-GT, GOT, ve CK Düzeylerine Etkisi. Etlik Veteriner Mikrobiyoloji Dergisi, 8(3), 90-103.