Anadolu Merinoslarında İrisin Hormon Yanıtı Üzerine Bazı Fizyolojik Parametrelerin Etkisi

Yıl 2021, Cilt: 32 Sayı: 2, 145 - 150, 30.12.2021

Bülent Bayraktar Emre Tekce

https://doi.org/10.35864/evmd.954977

Öz

Bu çalışmanın amacı, Anadolu Merinosu koyunlarında irisin hormon yanıtı üzerinde vücut kondüsyon skoru (VKS), laktasyon, gebelik, yaş ve cinsiyetin etkisinin incelenmesidir. Çalışmaya dahil edilen tüm gruplarda yer alan Anadolu Merinosu koyunları vücut kondüsyon skorlarına (VKS<2,VKS=3-3.5,VKS≥4) göre 3 gruba ayrılmıştır. 1.grup gebeliğin ilk dönemindeki koyunlar (ilk gebeliğin 100. gününde) (n=30), 2.grup erken laktasyon döneminde bulunan koyunlar (n = 30),3.grup farklı yaşta bulunan <2 (genç) (n=30), 2-8 (ergin) (n=30), 8> (yaşlı) (n=30), erkek ve dişi koyunlar (n=180) olmak üzere toplam 240 hayvan oluşturdu. Koyunlardan sabah yemlemesi öncesi vena jugularis'ten kan örnekleri alındı. Kan serumu örneklerinde ELİSA yöntemiyle irisin hormon düzeyleri belirlendi. Çalışma sonucunda laktasyon ve gebelik döneminde ortalama serum irisin hormon düzeyleri en düşük VKS<2 grubunda, en yüksek ise VKS ≥4 gruplarında bulunan koyunlarda saptanmıştır. Aynı şekilde farklı yaş ve cinsiyet gruplarında ise en düşük ortalama serum irisin hormon düzeyleri VKS<2 grubunda bulunan genç kuzu ve toklularda iken, en yüksek düzey ise VKS ≥4 grubunda yaşlı koyun ve koçlarda tespit edilmiştir. Sonuç olarak, VKS değişimine bağlı olarak farklı yaş, cinsiyet, gebelik ve laktasyon dönemlerinin serum irisin hormonu düzeyi üzerine etkisi olduğu belirlenmiştir (P<0,05).

Anahtar Kelimeler

Cinsiyet, gebelik, irisin, koyun, laktasyon, vücut kondüsyon skoru, yaş

Effect of Some Physiological Parameters on The Hormone Response of İrisin in Anatolian Merino Sheep

Öz

The aim of this study was to examine the effect of body condition score (BCS), lactation, pregnancy, age and gender on the hormone response of irisin in Anatolian Merino sheep. Anatolian Merino sheep in all groups included in the study were divided into 3 groups according to their body condition scores (BCS<2, BCS=3-3.5, BCS≥4). 1st group, sheep in the first period of pregnancy (100 of the first pregnancy (n=30), 2nd group, of sheep found in early lactation period (n = 30), 3rd group consisted of <2 (young) (n=30), 2-8 (adult) (n=30), 8> (old) (n=30) ewe (n = 90) and rams (n = 90) were of different ages consisted of a total of 240 animals. Blood samples were taken from vena jugularis before the morning feeding of the sheep. Hormone levels of the iris were determined by ELISA method in blood serum samples. As a result of the study, mean serum irisin hormone levels during lactation and pregnancy were found in sheep in the lowest BCS<2 Group and the highest BCS ≥4 groups. In the same way, in different age and gender groups, the lowest mean serum irisin hormone levels were found in young lambs and Toks in the BCS<2 group, while the highest level was found in older sheep and Rams in the BCS ≥4 group. As a result, it was determined that different age, gender, pregnancy and lactation periods had an effect on serum irisin hormone level depending on BCS change (P<0.05).

Anahtar Kelimeler

Age, body condition score, gender, irisin, lactation, pregnancy, sheep

Kaynakça

• Aydın S, Kuloglu T, Aydin S.2013.Copeptin, adropin and irisin concentrations in breast milk and plasma of healthy women and those with gestational diabetes mellitus. Peptides. 47, 66-70.
• Bayraktar B, Tekce E, Aksakal V, Takma Ç, Bayraktar FG, Şengül B. (2020). Effects of Race, Gender, Body Condition Score and Pregnancy on Serum Apelin Levels in Ewe. J. Agric. Sci. 26(3), 363-372.
• Bienertová-Vašků J, Bienert P, Zlámal F, Tomandl J, Tomandlová M, Dostálová Z, Vašků A. (2012). Visfatin is secreted into the breast milk and is correlated with weight changes of the infant after the birth. Diabetes Res Clin Pract. 96(3), 355-361.
• Boström, P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC, Spiegelman BM. (2012). A PGC1α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature, 481(7382), 463-468.
• Garcés MF, Peralta JJ, Ruiz-Linares CE, Lozano AR, Poveda NE, Torres-Sierra AL, Caminos JE. (2014). Irisin levels during pregnancy and changes associated with the development of preeclampsia. The Journal of Clinical Endocrinology & Metabolism. 99(6), 2113-2119.
• Gawlinska K, Gawlinski D, Przegalinski E, Filip M. (2019). Maternal high-fat diet during pregnancy and lactation provokes depressive-like behavior and influences the irisin/brain-derived neurotrophic factor axis and inflammatory factors in male and female offspring in rats. J. Physiol. Pharmacol. 70, 407-411.
• Ebert T, Stepan H, Schrey S, Kralisch S, Hindricks J, Hopf L, Fasshauer M. (2014). Serum levels of irisin in gestational diabetes mellitus during pregnancy and after delivery. Cytokine. 65(2), 153-158.
• Huh JY, Panagiotou G, Mougios V, Brinkoetter M, Vamvini MT, Schneider BE, Mantzoros CS. (2012). FNDC5 and irisin in humans: I. Predictors of circulating concentrations in serum and plasma and II. mRNA expression and circulating concentrations in response to weight loss and exercise. Metabolism. 61(12), 1725-1738.
• Khan K. (1993). Effects of Body Condition and PreLambing Supplementation on Ewe Productivity. MS Thesis, Oregon State University, 102p, Oregon, USA.
• Lee HJ, Lee JO, Kim N, Kim JK, Kim HI, Lee YW, Kim HS. (2015). Irisin, a novel myokine, regulates glucose uptake in skeletal muscle cells via AMPK. Mol. Endocrinol.29(6), 873-881.
• Mahgoub MO, D’Souza C, Al Darmaki RS, Baniyas MM, Adeghate E. (2018).An update on the role of irisin in the regulation of endocrine and metabolic functions. Peptides.104, 15-23.
• Martinez Munoz IY, Camarillo Romero EDS, Garduno Garcia JDJ. (2018).Irisin a novel metabolic biomarker: present knowledge and future directions. Int. J. Endocrinol 2018,1-9.
• Mól N, Zasada M, Tomasik P, Klimasz K, Kwinta P. (2018). Evaluation of irisin and visfatin levels in very low birth weight preterm newborns compared to full term newborns-A prospective cohort study. PloS one. 13(9),e0204835.
• Palacios‐González B, Vadillo‐Ortega F, Polo‐Oteyza E, Sánchez T, Ancira‐Moreno M, Romero‐Hidalgo S, Antuna‐Puente B. (2015). Irisin levels before and after physical activity among school‐age children with different BMI: A direct relation with leptin. Obesity. 23(4), 729-732.
• Roca-Rivada A, Castelao C, Senin LL, Landrove MO, Baltar J, Crujeiras AB, Pardo M. FNDC5/irisin is not only a myokine but also an adipokine. PloS one, 8(4), e60563.
• Ruan Q, Huang Y, Yang L, Ruan J, Gu W, Zhang X, Yu Z. (2019). The effects of both age and sex on irisin levels in paired plasma and cerebrospinal fluid in healthy humans. Peptides.113, 41-51.
• Ruan Q, Yang L, Ruan J, Gu W, Zhang Y, Bao Z, Yu Z. (2017). Age-Related Changes Of Irısın Levels In Plasma And Cerebrospınal Fluıd Of Humans. Innovation in Aging. 1(1), 138-138.
• Shuttleworth TJ. (1997).Intracellular Ca2+ signalling in secretory cells. J. Exp. Biol. 200(2):303-314.
• So WY, Leung PS. (2016).Irisin ameliorates hepatic glucose/ lipid metabolism and enhances cell survival in insulinresistant human HepG2 cells through adenosine monophosphate-activated protein kinase signaling. Int J Biochem Cell Biol. 78,237–247.
• Stanford KI, Middelbeek RJ, Townsend KL, An D, Nygaard EB, Hitchcox KM, Goodyear LJ. (2012).Brown adipose tissue regulates glucose homeostasis and insulin sensitivity. J Clin Invest. 123(1), 215-223.
• Thompson J, Meyer H. (1994).Body Condition Scoring of Sheep. Oregan State University Extension Servise;1-4.
• Thompson JM, Meyer H. (2006).Body Condition Scoring of Sheep. Proc. 52nd Bien. Spooner Sheep D. 2006; 28p.
• Townsend LK, Wright DC. (2019). Looking on the “brite” side exercise-induced browning of white adipose tissue. Pflugers Arch. 471(3), 455-465.
• Zügel M, Qiu S, Laszlo R, Bosnyák E, Weigt C, Müller D, Schumann U. (2016). The role of sex, adiposity, and gonadectomy in the regulation of irisin secretion. Endocrine, 54(1), 101-110.