Monoklanal Antikorların Newcastle Hastalığının Teşhis ve Epidemiyolojisinde Kullanımı

Yıl 1999, Cilt: 10 Sayı: 1, 63 - 78, 01.06.1999

Taner Öncel

Öz

Poliklonal antikorların konvansiyonel serolajik testlerde virusların
ayrımında kullanılması bazı problemlere yol açmaktadır. Monoklonal
antikor teknolojisi (mAb) konu için yeni bir alternatif olmuştur.
Newcastle Hastalığı Viruslarına (NHVJ karşı hazırlanan mAb'ler
değişik izolatlar arasındaki kayda değer antijenik farklılığı göstermişlerdir.
NHV suşlarının hepsi yada pek çoğu için spesifik olan ve HI aktivitesi
gösteren mAb'ler. Newcastle Hastalığı (NH) için referans laboratuvar
olan Weybridge'de rutin teşhiste kullanılmaktadır. Bu
mAb'lerin kullanımı NHV. variant NHV. Lasota ve PMV-3 arasında ayrımı
sağlamaktadır.
MAb'ler aynı zamanda NHV'larının antijenik yapılarına göre
gruplanmalarını sağlamışlardır. Bu konudaki en başarılı yaklaşım
Russell ve Alexander ' ın çalışmaları olmuştur. Araştırıcılar, NHV'larını,
NHV Ulster 2C suşuna karşı oluşturulan 9 mAb ile reaksiyonlarını
temel alarak gruplamışlardır. Oluşturulan grupların virulans, temel
konakçı gibi epidemiyolojikal özellikleri büyük ölçüde paylaştığı
görülmüştür. MAb gruplamasının NH'nin epidemiyolojisindeki başarısı.
İngiltere'de variant NHV'larının tavuklarda oluşturduğu NH salgınlarını
ve Türkiye'de Güney Marmara bölgesinde son zamanlarda
görülen NH salgınını da içeren pek çok olguda kanıtlanmıştır.

Anahtar Kelimeler

Etlik Veteriner Mikrobiyoloji Dergisi, Newcastle, Monoklonal Antikorlar, Epidemiyoloji, 1999

Use of Monoclonal Antibiotics in Diagnosis And Epidemiology or Newcastle Disease

Öz

Applications of monoclonal an tibody (mAb) technology for Virus
characterisation has been an alternative approach since polyclonal
antibodies have been shown to have some problemsin discriminating
Viruses in conventional serological tests . MAbs to Newcastle disease
virus (NDVJ have proved the considerable variation among different
isola tes. Many laboratories have prepared mAbs to NDVs and shown
conserved and variant epitopes on some isolates.
MAbs that is spesific for all or vast majority of NDV strains and
show HI actiVities are now being used in Weybridge. international referans
laboratory for Newcaslle Disease (ND), for routine diagnosis.
Use of these mAb's enables distinction between NDV, variant NDV.
Lasota and PMV-3 Viruses.
MAbs also served to group NDVs with regard to their antigenic
properties. The most successful approaches on this subject were studies
of Russell and Alexander. They grouped NDV strains on the hasis
of their reaction with 9 mAbs to NDV Ulster 2C. The groups formed
have shared im portant epidemiological properties, such as main
host, virulance ete. Success of mAb grouping in epidemiology of the
ND has been confirmed in many ND casesincluding ND outbreakin
chickens due to variant NDVs in England and recent ND outbreakin
south Marmara region of Turkey.

Kaynakça

• ABENES G, KIDA H, YANAGAWA R, (1986). Antijenilc mapping andjunctional analysis oj the F protein oj Newcastle Disease virus using Monocianal Antibodies. Arch. Virol. 90 97-110
• ALEXANDER DJ, (1980). Avian Paramyxovirusses VEt. Bull. 50: 537-572
• ALEXANDER DJ, (1985). Antijenic and biological characterization oj Avian Paramyxoirusses. Type-1 isolatesfrom pigeons-an international collabarative study. Avian Pathol. 14:365-376
• ALEXANDER DJ, (1990). Avian Paramyxoviridae-Recent developments. Vet. Mic. 23. 103-114
• ALEXANDER DJ, (1991). Newcastle Disease and other paramyxovirus injections. Diseases ofpoultry, 9. Edition, Iowa state university press lowa. 1101-1108.
• ALEXANDER DJ, (1992). Use oj monocianal antibodies in the diagnosis oj Newcastle disease and characterization oj the viruses. W arkshop on Avian Paramyxoviruses. Edition, Commission of the European communities 145-156
• ALEXANDER DJ, (1995). Neewcastle Disease in countries oj the European Union. Avian Pathol. 24 3-10
• ALEXANDER DJ, PARSONS G, MARSHALL R, (1984). Injection oj jowls with Newcastle disease virus by jood contaminated with pigeonjaeces Vet. Rec. 115: 601-602
• ALEXANDER D J, WILSON GW C, RUSSEL P H, LISTER S A, PARSONS G, (1985). Newcastle Disease outbreaks injowl in Great Britain during 1984 Vet. Rec. 117: 429-434
• ALEXANDER D J, MACKENZIE J S, RUSSELL P H, (1986). Two types oj Newcastle disease virusses isolatedjromjeral birds in Western Australia detected by monocianal antibodies. Aust. Vet. J. 63: 365-367
• ALEXANDER DJ, MANVELL RJ, KEMP PA, PARSON S G, COLLYNS M S, BRACK MAN S, RUSSELL PH, LISTER SA. ( 1987). Use oj monocianal antibodies in the characterization oj Av ian paramyxovirusses type 1 isolates submitted to an iriternational rejerence laboratory. Avian Pathol. 16: 553-565
• ALEXANDER DJ, MANVELL RJ, COLLINS M S, BROCKMAN S J, (1991). Evaluation oj relationships between Avian paramyxovirusses isolatedjrom birds oj the family Columbidae. Arch. Virol 116: 267-276
• ALEXANDER D J, CAMPBELL G, MANVELL R J, COLLINS M S, PARSONS G, MC NULTY M S, (1992). Characterization oj an antigenically unusual virus responsible for two outbreaks oj Newcast/ e disease in the Republic oj Ireland in 1990. Vet. Rec. 130: 65-68
• ALEXANDER DJ, MANVELL RJ, LOWINGS J P, FROST K M, COLLINS M S, RUSSELL PH, SMITH J E, (1997). Antigenic diversity and similarities detected in Paramyxovirus type 1 (Newcastle disease virus) isolates using monocianal antibodies. AVian Pathol. 36: 399-418
• ALLAN W H, LANCESTER J E, TOTH B, (1978). Newcastle disease vaccines: food and agriculture organizations of the United Nations. Rome.
• ANDERSON C, KEARSLEY R, ALEXANDER D J, RUSSELL PH, (ı987); Antigenic variation in Avian paramyxovirus type 3 isolates detected by mouse monocianal antibodies. AVian Pathol. 16: 691-698
• COLLIN M S, ALEXANDER DJ, BRACKMAN S, KEMP D A, MANVELL R S, (1989). Evaluation oj mouse monocianal antibodies raised against an isoiate oj the variant avian Paramyxovirus type 1 responsiblejor the current panzootic in pigeons. Arch. Virol. 104: 53- 61
• ERDEI J, ERDEI K, KOCHIR K, KALETA E F, SHORTRIDGE K F, LOMNICCI B, (1987). Newcastle disease vaccine (Lasota) spesific monocianal antibody. Arch. Virol. 96:265-269
• FEVERIO M, (1993). Newcastle disease in Portugal: Situation report- October 1993. Proceedings oj the jointjirst annual meetings oj the national Newcastle disease and Avian Injluenza Iabarataires oj the European communities. Edited by D.J.Alexander 66
• HOSHI S, MIKAMI T, NAGATA K, ONUMA M, IZOWA H, (1983). Monocianal antibodies against a paramyxovirus isolatedfrom Japanase sporrow Hawlcs Arch. Virol. 85:109-121
• ISHIDA M, NEROME K, MATSUMATO M, MIKAM, T, OYA A, (1985). Characterization oj rejerance strains oj Newcastle Disease virus and NDV li/ce isolates by monocianal antibodies to HN subunits. Arch . Virol. 85:112-115
• IORIO RM, GLICKMAN R H, SHEEKAN P J, (1992). Inhibition oj jusion by neutralizing monocianal antibodies to HN glycoprotein oj NDV. J. Gen.Virol. 1167-1177
• JESTIN V, CHERBONEL M, MORIN M, GUITTET M, BENNEJEAN G, (1989). Characterization oj French avian paramyxovirus type-1 (PMV-1) isolates with a panel oj monocianal antibodies to Plujragan strains oj NDV. Arch. Virol. 105:189-198
• KALETA E F, ALEXANDER DJ, RUSSELL PH, (1985). Thejirst isolation oj the Avian PMV-1 virus responsiblejor the current panzootic in pigeons. Avian Pathol. 14 : 553-557
• LANCESTER J E, ALEXANDER DJ, (1975) . Newcastle disease virus and spread, Canada Dep. Of Agriculture Monograph no11 Ottowa
• MEULEMANS G, GONZE M, CARLIER M C, PETIT P, BURNY A, LONG L, (1986). Antigenic and biological characterisation oj Avian paramyxovirus type-1 isolatesfrom pigeons. Arch. Virol. 87: 151-161
• MEULEMANS G, GONZE M, CARLIER M C, PETIT P, BURNY A, LONG L, (1986). Protective ejjects oj HN and F glycoprotein spesific monocianal antibodies on experimental Newcastle disease. Avian Pathol. 19:761-768
• MEULEMANS G, GONZE M, CARLIER M C, PETIT P, BURNY A, LONG L, ( 1987). Evaluation oj the use oj monocianal antibodies to Hemaglutinin andjusion glycoproteins oj NDV for virus identijication and strain dijferentiation purposes. Arch. Virol. 92:55-62
• NISHIKAWA K, HANADA N, MORISHIMA T, YOSHIDA T, HAMAGUCHI M, TOYODA M, NAGAI Y, (1987). Antigenic characterisation oj internal proteins oj Newcastle disease virus by monocianal antibodies. Virus research. 7 :8 3 .92
• ÖNCEL T, ALEXANDER D J, MANVELL R J, TURE O, ( 1997). Characterization oj Newcastle disease virusses isolatedjrom chiclcens and pigeons in south Marmara region oj Turkey. Avian Pathal. 26:129-137
• ÖZDEMİR Y, RUSSELL P H, COLLYER J, ALEXANDER D J, MANVELL RJ, (1990). Monocianal antibodies to Avian paramyxovirus type-2. Avian Pathol. 19: 395-400
• RUSSELL P H, (1988). Monocianal antibodies in research, diagnosis and epizootiology oj Newcastle disease. Newcastle disease ed. by D.J.Alexander., Kluwer acad. Publ., London 137-146
• RUSSELL PH, GRIFFITITHS PC, GOSWAMI KA, ALEXANDER DJ, CANNON MJ, RUSSELL W C, (1983). The characterization oj monocianal antibodies to Newcastle disease virus. J.Gen. Virol. 64:2069-2072
• RUSSELL PH, ALEXANDER DJ, (1983). Antigenic variation oj Newcastle disease virus strains detected by monocianal antibodies. Ardı. Viral. 75:243-253
• SRINIVASAPPA GB, SNYDER D B, MARQUART W W, KING DJ, (1986): Isolation oja monocianal antibody with a specifity for commonly employed vaccine strains oj Newcastle disease virus . Avian Dis. 562-567
• UMINO Y, KOHOMA T, SATO TA, SUGIURA A, KLENK H D, ROTT R, ( 1990): Monocianal antibodies to three structural proteins of Newcastle disease virus: Biological characterization with particu/ar rejerance to the coriformational change oj envelope glycoproteins associated w ith proteolytic cleavage. J . Gen. Viral. 71:1189-1197
• UMINO Y, KOHOMA T, SATO T A, SUGIURA A, (1983): Protective ejject oj monocianal antibodies to Newcastle disease virus in passive immunization. J.Gen. Viral. 71:1199-1203