Kobaylarda Deneysel Toxoplasma gondii Enfeksiyonunda Patolojik Bulgular

Yıl 2001, Cilt: 12 Sayı: 1-2, 23 - 38, 01.12.2001

Ouuz Kul Rıfkı Hazıroglu

Öz

Bu çalışmada, 42 adet erkek kobayda, farklı yollarla Toxoplasma gondii Ankara suşu takizoitleri verilerek deneysel toksoplazmozis oluşturuldu. Her grupta 12 ko bay olacak şekilde; intravenöz (grup 1 ), intraperitoneal (grup 2) ve intradermal (grup 3) yollarla enfekte edilen üç deney grubu oluşturuldu. Ayrıca her bir deney grubu için ikişer kobay kontrol (grup 4) olarak ayrıldı. İnokülasyon sonrası belirlenen günlerde örenazileri yapılan kabaylardan alınan sistemik doku örnekleri histopatolojik ve elektron mikroskobik yönden incelendi.
Enfeksiyonda şekillenen lezyonlar etkeni n veriliş yoluna göre farklılık gösterdi. Makroskobik olarak, grup 1 'de yoğun olarak akciğerler, karaciğer ve böbrek üstü bezlerinde 1-2 mm büyüklüğünde çok sayıda boz-beyaz renkli odaklar şekillendi. Grup 2'de, karın boşluğunda değişen miktarlarda fıbrinli eksudatla birlikte organların yüzeyinin fıbrinle kaplandığı, grup 3'te ise deri inakülasyon bölgesinde 1.5-2.0 cm çapında kanama ve nekrozlar ile bölgesel lenf düğümlerinde nekrozlara rastlandı. Mikroskobik olarak; grup 1 'de akciğer, karaciğer, dalak ve böbrek üstü bezlerinde T. gondii takizoit fonnlarının bulunduğu multifokal pıhtılaşma nekrozları gözlendi. Grup
2'de karaciğer, dalak, bağırsak ve böbreklerin serozalarından başlayarak parankimine doğru ilerleme gösteren nekrozlar şekillendi ve çok sayıda serbest takizoit grupları gözlendi. Grup 3'te ise deri, lenf düğümleri, karaciğer, beyin ve akciğerde takizoitlerin de bulunduğu granülomatöz lezyonlar şekillendi. İnokülasyon sonrası aradan geçen süreyle doğru orantılı olarak, lezyonların organlardaki yaydışında artış görüldü. Elektron mikroskobik incelemede; hücre dışında serbest takizoitlerle birlikte dokularda yoğun nekroz dikkati çekti. Hücre içi takizoitlere ise çoğunlukla makrofaj sitoplaznıalarında rastlandı ve endozoit fom1ları da gözlendi.

Anahtar Kelimeler

Oğuz KUL, Rıfkı HAZIROGLU, 2001, Etlik Veteriner Mİkrobiyoloji Dergisi, Deneysel toksoplazmozis, elektron mikroskopi, kobay, patoloji

Pathological Findings of Experimental Toxoplasma gondii Infection in Guinea Pigs

Öz

An experimental toxoplasınosis was performed via different routes in 42 male guinea pigs by
adıninistı·ating tachyzoites of Toxoplasma gondii Ankara strain. Three experimental groups were constituted by
administratiııg tachyzoites through intravenous (group I), iııtraperitoneal (group 2), intradermal (group 3) routes.
Each group contained 12 guinea pigs and 2 guinea pigs for each group were used to set up control (group 4).
Tissue specimens of the euthanasied guinea pigs on certain days after inoculation were collected and examined
histopathologically and ultrastructurally. The lesions occurred, were different in location and histopathological
features depending on the inoculation routes. Macroscopically; In group I, multifocal gray to white foci were
observed intensively in the lungs, liver and adreııal glaiıds of aııimals. In group 2, the serosal surfaces of abdominal cavity organs were covered with fıbrine , and in group 3, necroti c and lıaemorrhagic areas
in 1.5-2.0 cm diameter at the inoculation si tes of the ski n were observed and region al lymph nodes were severely
affected. Microscopically; In group 1, ınultifoc a l coagulation necrosis containing T. gondii tachyzoites were
found in the lung, liver, splcen and adrenal gland . In group 2, necrotic areas starting at the seraza of the li ver,
spleen, intestine and kidney were found to invade through the parenchyma. In this group, abundant tachyzo ites
were detected. In group 3, granulomatous lesions included tac lıyz o it es were frequently seen in the skin, lymph
node, liver, brain and lung. According to post inoculation days, lesions were distributed and invaded to the deep
parts of the organs.
Ultras tructurally; intensive necrotic areas with extracellular free tachyzoites were observed in exaınined
ti ssues. Intracellul ar tachyzoites were detected frequcntly in ınacroph age cytoplasıns and endozoite forıns were
alsa found.

Anahtar Kelimeler

Experiınental tox oplasınosis, guinea pig, pathology, ultrastructure

Kaynakça

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