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A comparative histopathological evaluation of acute toxicities associated with Scillaren-A, corn cob powder and their combinations in rats

Yıl 2022, Cilt: 33 Sayı: 1, 1 - 11, 15.06.2022
https://doi.org/10.35864/evmd.1050708

Öz

The purpose of the study was to comparatively evaluate the efficacy of Scillaren-A in rats. A total of 32 female Wistar albino rats were used to develop acute toxicity for 48 h. The rats were randomly divided into four groups including n=8 / each group (G.I-G.IV). Toxicity groups (G.I, G.II, and G.III) and control group (G.IV) were established. The experimental groups were intoxicated with scillaren A, corn cob powder, and their combinations, respectively. The rats in the control group were remained intoxicated and fed with standard pellets. The experiment was ended 48 hours after the intoxication. Histopathological findings in vital organs (liver, kidney, lung, heart, brain) and then other organs (intestine, spleen) were evaluated. Tissues were fixed in 10% buffered formalin (pH=7.2) and embedded in paraffin. The histopathologic findings were evaluated on the standard H&E stained sections. The major histopathological changes, vascular changes including hyperemia and haemorrhagia, alterative changes including degeneration and necrosis, inflammation, some regenerative and reparative changes including hyperplasia and fibrosis in organs were scored. It is believed that both formulations can be effective on death later on, be utilized as a rodenticide without environmental toxic effect, and be consumed rapidly by rats due to their attractive chemical traits.

Destekleyen Kurum

Supported by KOSGEB Project.

Proje Numarası

2018-701-13

Kaynakça

  • Aktaş İ, Özmen Ö, Tutun H, Yalçın A, Türk A. (2020) Artemisinin attenuates doxorubicin induced cardiotoxicity and hepatotoxicity in rats. Biotech Histochem. 95,121-128. doi: 10.1080/10520295.2019.1647457.
  • Alp H, Tutun H, Kaplan HM, Şingirik E, Altintaş L. (2019) Investigation of apoptotic effects of hypericum perforatum extract on breast cancer cell line. Harran Üniv Vet Fak Derg 8, 198-202. doi: 10.31196/huvfd.667748.
  • Bălăşoiu L, Călina D, Docea A, Patru E, Valse E, Maria B. (2013) Determination of cardiac glycosides in Scilla bifolia (Liliaceae) by two different analytical techniques: Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS). J Med Plants Res. 7, 3131-3138. doi:10.3906/zoo-1611-32.
  • Bayazıt V, Konar V. (2010) Analgesic effects of scilliroside, proscillaridin-a and taxifolin from squill bulb (Urginea maritima) on pains. Digest J Nanomaterial Biostruct. 5, 465-467.
  • Bilir EK, Tutun H, Sevin S, Kismali G, Yarsan E. (2018) Cytotoxic effects of Rhododendron ponticum L. extract on prostate carcinoma and adenocarcinoma cell line (DU145, PC3). Kafkas Üniv Vet Fak Derg. 24, 451-457. doi: 10.9775/kvfd.2017.19219.
  • Brooks JE, Htun PT. (1980) Laboratory evaluation of scilliroside used as a rodenticide against the lesser bandicoot rat, Bandicota bengalensis. J Hygiene. 85,227-234. doi:10.1017/S0022172400063269.
  • Gentry HS, Verbiscar AJ, Banigan TF. (1987) Red squill (Urginea maritima, Liliaceae). Economic Botany. 41, 267-282. doi: 10.1007/BF02858974.
  • Gratz NG. (1973) A critical review of currently used single-dose rodenticides. Bull WHO. 48, 469-477.
  • Gupta RC. (2018) Non-Anticoagulant Rodenticides. Gupta RC eds. Veterinary Toxicology. Third edition. USA: Academic Press; p. 613-626.
  • Jacob J, Buckle A. (2018) Use of anticoagulant rodenticides in different applications around the world. Anticoagulant Rodenticides and Wildlife. Springer, Cham.USA p. 11-43. doi: 10.1007/978-3-319-64377-9_2.
  • Kasote DM, Katyare SS, Hegde MV, Bae H. (2015) Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci. 11, 982. doi: 10.7150/ijbs.12096.
  • Keyvan E, Tutun H. (2019) Effects of carvacrol on Staphyloccus aureus isolated from bulk tank milk. Medycyna Weterynaryjna-Vet Med Sci Pract. 75, 238-241. doi: 10.21521/mw.6211.
  • Luna LG. (1968) Manual of histologic staining methods of the armed forces institute of pathology. Third edition. New York USA: McGrawHill.
  • Mahlo SM, Chauke HR, McGaw L, Eloff J. (2016) Antioxidant and antifungal activity of selected medicinal plant extracts against phytopathogenic fungi. Afr J Tradit Complement Altern Med. 13, 216-222. doi: 10.21010/ajtcam.v13i4.28.
  • Perry SC. (2013) U.S. Patent No. 8,574,638. Washington, DC: U.S. Patent and Trademark Office. 2013.
  • Sikarwar MS, Patil MB. (2014) Antihyperlipidemic activity of Pongamia pinnata leaf extracts. Turk J Pharmaceut Sci. 11, 329-338.
  • Tanker M, Tosun F. (1979) Ege yöresinde yetişen Urginea maritima (L.) Bak. soğanlarının Prosillaridin yönünden değerlendirilmesi: Evaluation of the Balbs of Urginea maritima (L.) Bak. Growing in the Aegean District from. J Facult Pharm Ankara Univ. 9, 1-11.
  • The Pesticide Manual. (1983) Worthing CR, Walker SB. eds. A World Compendium. Seventh edition. British Crop Protection Council, England: Croydon; p. 697.
  • Tobyn G, Denham A, Whitelegg M. (2010) The western herbal tradition: 2000 years of medicinal plant knowledge. Singing Dragon, UK: Churchill Livingstone; p. 1-392. doi:10.1016/B978-0-443-10344-5.00020-3.
  • Tutun H, Koç N, Kart A. (2018) Plant essential oils used against some bee diseases. Turkish J Agricult-Food Sci Tech. 6, 34-45. doi: 10.24925/turjaf.v6i1.34-45.1502.
  • Ujváry I. (2010) Pest control agents from natural products. Kriger R. eds. Hayes' Handbook of Pesticide Toxicology. USA: Academic Press; p. 119-229. doi: 10.1016/B978-0-12-374367-1.00003-3.
  • Verbiscar AJ, Patel J, Banigan TF, Schatz RA (1986a). Scilliroside and other scilla compounds in red squill. J Agricult Food Chem. 34, 973-979. doi: 10.1021/jf00072a011.
  • Verbiscar AJ, Banigan TF, Gentry HS. (1986b) Recent research on red squill as a rodenticide. Salmon TP. eds. Proceedings of the 25th Vertebrate Pest Conference. University of California, USA: Davis; p. 51-56.
  • Xu S, Wang G, Guo R, Zhang J. 2019. Extraction of steviol glycosides from Stevia rebaudiana (Bertoni) leaves by high‐speed shear homogenization extraction. J Food Proc Preserv. 43(12), e14250. doi: 10.1111/jfpp.14250.
  • Yadav PB, Lekhak UM, Ghane SG, Lekhak MM. (2020) Phytochemicals, antioxidants, estimation of cardiac glycoside (Scillaren A) and detection of major metabolites using LC-MS from Drimia species. South African J Botany. doi: 10.1016/j.sajb.2020.05.002.
  • Zhao J, Zhang W, Zhang X, Zhang X, Lu C, Deng Y. (2013) Extraction of cellulose nanofibrils from dry softwood pulp using high shear homogenization. Carbonhydr Polymer. 97: 695-702. doi: 10.1016/j.carbpol.2013.05.050

Ratlarda Scillaren-A, mısır koçanı tozu ve kombinasyonları ile ilişkili akut toksisitelerin karşılaştırmalı histopatolojik değerlendirilmesi

Yıl 2022, Cilt: 33 Sayı: 1, 1 - 11, 15.06.2022
https://doi.org/10.35864/evmd.1050708

Öz

Çalışmanın amacı Scillaren-A’nın ratlarda etkinliğini karşılaştırmalı olarak değerlendirmekti. Kırk sekiz saat boyunca akut toksisite geliştirmk için toplam 32 dişi Wistar albino rat kullanıldı. Ratlar rastgele dört gruba ayrıldı (her grupta n=8 / G.I-G.IV). Toksisite grupları (G.I, G.II ve G.III) ve kontrol grubu (G.IV) oluşturuldu. Deney grupları sırasıyla scillaren A, mısır koçanı tozu ve bunların kombinasyonu ile zehirlendi. Kontrol grubundaki ratlar zehirlenmedi ve standart peletler ile beslendi. Toksikasyondan 48 saat sonra deney sonlandırıldı. Yaşamsal organlarda (karaciğer, böbrek, akciğer, kalp, beyin) ve ardından diğer organlarda (bağırsak, dalak) histopatolojik bulgular değerlendirildi. Dokular %10’luk tamponlu formalin (pH=7.2) içinde tespit edildi ve parafine gömüldü. Histopatolojik bulgular standart H&E boyalı kesitlerde değerlendirildi. Organlardaki önemli histopatolojik değişiklikler, hiperemi ve hemorajiyi içeren vasküler değişiklikler, dejenerasyon ve nekrozu içeren alteratif değişiklikler, yangı, hiperplazi ve fibrozis gibi bazı rejeneratif ve reperatif değişiklikler skorlandı. Her iki formülasyonun da ilerleyen zamanda ölüm gelişmesinde etkili olabileceğine, çevresel toksik etkisi olmaksızın bir rodentisit olarak kullanılabileceğine ve çekici kimyasal özellikleri nedeniyle ratlar tarafından hızla tüketilebileceğine inanılmaktadır.

Proje Numarası

2018-701-13

Kaynakça

  • Aktaş İ, Özmen Ö, Tutun H, Yalçın A, Türk A. (2020) Artemisinin attenuates doxorubicin induced cardiotoxicity and hepatotoxicity in rats. Biotech Histochem. 95,121-128. doi: 10.1080/10520295.2019.1647457.
  • Alp H, Tutun H, Kaplan HM, Şingirik E, Altintaş L. (2019) Investigation of apoptotic effects of hypericum perforatum extract on breast cancer cell line. Harran Üniv Vet Fak Derg 8, 198-202. doi: 10.31196/huvfd.667748.
  • Bălăşoiu L, Călina D, Docea A, Patru E, Valse E, Maria B. (2013) Determination of cardiac glycosides in Scilla bifolia (Liliaceae) by two different analytical techniques: Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography-Mass Spectrometry (HPLC-MS). J Med Plants Res. 7, 3131-3138. doi:10.3906/zoo-1611-32.
  • Bayazıt V, Konar V. (2010) Analgesic effects of scilliroside, proscillaridin-a and taxifolin from squill bulb (Urginea maritima) on pains. Digest J Nanomaterial Biostruct. 5, 465-467.
  • Bilir EK, Tutun H, Sevin S, Kismali G, Yarsan E. (2018) Cytotoxic effects of Rhododendron ponticum L. extract on prostate carcinoma and adenocarcinoma cell line (DU145, PC3). Kafkas Üniv Vet Fak Derg. 24, 451-457. doi: 10.9775/kvfd.2017.19219.
  • Brooks JE, Htun PT. (1980) Laboratory evaluation of scilliroside used as a rodenticide against the lesser bandicoot rat, Bandicota bengalensis. J Hygiene. 85,227-234. doi:10.1017/S0022172400063269.
  • Gentry HS, Verbiscar AJ, Banigan TF. (1987) Red squill (Urginea maritima, Liliaceae). Economic Botany. 41, 267-282. doi: 10.1007/BF02858974.
  • Gratz NG. (1973) A critical review of currently used single-dose rodenticides. Bull WHO. 48, 469-477.
  • Gupta RC. (2018) Non-Anticoagulant Rodenticides. Gupta RC eds. Veterinary Toxicology. Third edition. USA: Academic Press; p. 613-626.
  • Jacob J, Buckle A. (2018) Use of anticoagulant rodenticides in different applications around the world. Anticoagulant Rodenticides and Wildlife. Springer, Cham.USA p. 11-43. doi: 10.1007/978-3-319-64377-9_2.
  • Kasote DM, Katyare SS, Hegde MV, Bae H. (2015) Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci. 11, 982. doi: 10.7150/ijbs.12096.
  • Keyvan E, Tutun H. (2019) Effects of carvacrol on Staphyloccus aureus isolated from bulk tank milk. Medycyna Weterynaryjna-Vet Med Sci Pract. 75, 238-241. doi: 10.21521/mw.6211.
  • Luna LG. (1968) Manual of histologic staining methods of the armed forces institute of pathology. Third edition. New York USA: McGrawHill.
  • Mahlo SM, Chauke HR, McGaw L, Eloff J. (2016) Antioxidant and antifungal activity of selected medicinal plant extracts against phytopathogenic fungi. Afr J Tradit Complement Altern Med. 13, 216-222. doi: 10.21010/ajtcam.v13i4.28.
  • Perry SC. (2013) U.S. Patent No. 8,574,638. Washington, DC: U.S. Patent and Trademark Office. 2013.
  • Sikarwar MS, Patil MB. (2014) Antihyperlipidemic activity of Pongamia pinnata leaf extracts. Turk J Pharmaceut Sci. 11, 329-338.
  • Tanker M, Tosun F. (1979) Ege yöresinde yetişen Urginea maritima (L.) Bak. soğanlarının Prosillaridin yönünden değerlendirilmesi: Evaluation of the Balbs of Urginea maritima (L.) Bak. Growing in the Aegean District from. J Facult Pharm Ankara Univ. 9, 1-11.
  • The Pesticide Manual. (1983) Worthing CR, Walker SB. eds. A World Compendium. Seventh edition. British Crop Protection Council, England: Croydon; p. 697.
  • Tobyn G, Denham A, Whitelegg M. (2010) The western herbal tradition: 2000 years of medicinal plant knowledge. Singing Dragon, UK: Churchill Livingstone; p. 1-392. doi:10.1016/B978-0-443-10344-5.00020-3.
  • Tutun H, Koç N, Kart A. (2018) Plant essential oils used against some bee diseases. Turkish J Agricult-Food Sci Tech. 6, 34-45. doi: 10.24925/turjaf.v6i1.34-45.1502.
  • Ujváry I. (2010) Pest control agents from natural products. Kriger R. eds. Hayes' Handbook of Pesticide Toxicology. USA: Academic Press; p. 119-229. doi: 10.1016/B978-0-12-374367-1.00003-3.
  • Verbiscar AJ, Patel J, Banigan TF, Schatz RA (1986a). Scilliroside and other scilla compounds in red squill. J Agricult Food Chem. 34, 973-979. doi: 10.1021/jf00072a011.
  • Verbiscar AJ, Banigan TF, Gentry HS. (1986b) Recent research on red squill as a rodenticide. Salmon TP. eds. Proceedings of the 25th Vertebrate Pest Conference. University of California, USA: Davis; p. 51-56.
  • Xu S, Wang G, Guo R, Zhang J. 2019. Extraction of steviol glycosides from Stevia rebaudiana (Bertoni) leaves by high‐speed shear homogenization extraction. J Food Proc Preserv. 43(12), e14250. doi: 10.1111/jfpp.14250.
  • Yadav PB, Lekhak UM, Ghane SG, Lekhak MM. (2020) Phytochemicals, antioxidants, estimation of cardiac glycoside (Scillaren A) and detection of major metabolites using LC-MS from Drimia species. South African J Botany. doi: 10.1016/j.sajb.2020.05.002.
  • Zhao J, Zhang W, Zhang X, Zhang X, Lu C, Deng Y. (2013) Extraction of cellulose nanofibrils from dry softwood pulp using high shear homogenization. Carbonhydr Polymer. 97: 695-702. doi: 10.1016/j.carbpol.2013.05.050
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Veteriner Cerrahi
Bölüm Araştırma Makaleleri
Yazarlar

Sedat Sevin 0000-0003-0475-9092

Mehmet Eray Alçığır 0000-0002-5165-5854

Mustafa Özkurt 0000-0002-1015-9344

Zekeriya Yurdabakan 0000-0002-0887-0874

Ömer Yalçın 0000-0002-7946-6465

Ender Yarsan 0000-0002-3008-9240

Proje Numarası 2018-701-13
Erken Görünüm Tarihi 24 Haziran 2022
Yayımlanma Tarihi 15 Haziran 2022
Gönderilme Tarihi 29 Aralık 2021
Yayımlandığı Sayı Yıl 2022 Cilt: 33 Sayı: 1

Kaynak Göster

APA Sevin, S., Alçığır, M. E., Özkurt, M., Yurdabakan, Z., vd. (2022). A comparative histopathological evaluation of acute toxicities associated with Scillaren-A, corn cob powder and their combinations in rats. Etlik Veteriner Mikrobiyoloji Dergisi, 33(1), 1-11. https://doi.org/10.35864/evmd.1050708


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