Türkiye’nin Ege Bölgesindeki gökkuşağı alabalığı (Oncorhynchus mykiss, Walbaum) anaçlarında bir hastalık etkeni Vagococcus salmoninarum

Yıl 2014, Cilt: 25 Sayı: 1, 11 - 16, 01.12.2014

Tansel Tanrıkul Meriç Lütfi Avsever Ertan Emek Onuk Behire Işıl Didinen

Öz

Türkiye’nin Ege Bölgesi’nde yer alan ticari bir alabalık çiftliğinde gökkuşağı alabalığı anaçlarında görülen bir
hastalık esnasında ortalama %10 oranında bir ölüm kaydedildi. Hastalık belirtileri yumurtlama sonrası 10-12°C su
sıcaklığında görüldü. Klinik olarak enfekte 2 anaç balıktan 6 adet bakteriyel izolat elde edildi. Bu izolatların Gr (+) kokobasil
oldukları tespit edildi ve fenotipik olarak Vagococcus salmoninarum olarak teşhis edildi. İzolatlar, aynı zamanda
spesifik V. salmoninarum primerleri ile PCR metodu kullanılarak teyit edildi. VG3 suşunun antibiyotik duyarlılığı
Kirby-Bauer disk difüzyon metodu ile belirlendi ve amoksisillin, ampisillin, enrofloksasin, norfloksasin, oksolinik asit
ve florfenikole duyarlılık gösterdi. Türkiye’de farklı coğrafik bölgelerden izole edilen V. salmoninarum izolatlarının
ERIC-PCR sonucuna göre ilişkili olduğu (0.73) görüldü.

Anahtar Kelimeler

Tansel TANRIKUL, Meriç Lütfi AVSEVER, Ertan Emek ONUK, Behire Işıl DİDİNEN, Oncorhynchus mykiss, fenotipik, genotipik, karakterizasyon, Vagococcus salmoninarum, 2014, Etlik Veteriner Mikrobiyoloji Dergisi

Vagococcus salmoninarum, a causative agent of disease in rainbow trout (Oncorhynchus mykiss, Walbaum) broodstocks in the aegean region of Turkey

Öz

In a commercial rainbow trout farm located in the Aegean region of Turkey, a disease outbreak with an average
mortality of 10% was recorded in rainbow trout broodstocks. The disease symptoms emerged at 10-12°C water temperature
after spawning periods. 6 bacterial isolates were obtained from 2 of the clinically infected rainbow trout broodstock.
These isolates were observed to be Gram-positive cocobacilli and were identified phenotypically as Vagococcus
salmoninarum. Isolates were also confirmed by using a PCR method with V.salmoninarum specific primers. Antibiotic
susceptibility of strain VG3 was assessed by the Kirby-Bauer disk diffusion method, and the results showed that it was
susceptible amoxycillin, ampicillin, enrofloxacin, norfloxacine, oxolinic acid and florfenicol. V.salmoninarum isolisolates
isolated from different geographical region in Turkey were revealed to be related (0.73) as a result of ERIC-PCR.
Key words: Oncorhynchus mykiss, phenotypic, genotypic, characterization, Vagococcus salmoninarum.

Anahtar Kelimeler

Oncorhynchus mykiss, phenotypic, genotypic, characterization, Vagococcus salmoninarum

Kaynakça

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