Laktasyon Periyodundaki Subklinik Mastitisli Koyunlarda Kkan ve Süt Laktoferrin Konsantrasyonlarının Araştırılması

Yıl 2013, Cilt: 24 Sayı: 2, 37 - 40, 01.12.2013

Cevat Nisbet Gül Fatma Yarım Gülay Çiftci Nilgün Gültiken Sena Çenesiz

Öz

Bu çalışmada, laktasyon periyodundaki subklinik mastitisli ve sağlıklı Karayaka ırkı koyunlarda süt ve kan serumlarında
laktoferrin konsantrasyonlarının belirlenmesi amaçlandı. Araştırmada, ortalama 2-3 yaşlarında ve laktasyonlarının
orta döneminde bulunan Karayaka ırkı koyunlar, Kaliforniya mastitis test ve sütte somatik hücre sayımı
yöntemleri ile subklinik mastitis yönünden incelendi. Sütleri Kaliforniya mastitis test pozitif ve somatik hücre sayımı
300.000-1.000.000 olan 23 adet koyun subklinik mastitis grubunu, Kaliforniya mastitis test negatif ve somatik hücre
sayımı <300.000 olan 20 adet koyun ise kontrol grubunu oluşturdu. İneklerden kan ve süt örnekleri alınarak serumları
çıkarıldı. Koyunların kan ve süt serumlarında laktoferrin ölçümleri ELISA test kitleri kullanılarak gerçekleştirildi.
Subklinik mastitisli ve sağlıklı koyunların serum laktoferrin değişimleri sırasıyla ile 0.90±0.08 μg/ml ve 0.85±0.08 μg/
ml olarak tespit edildi ve gruplar arasında istatistiksel olarak önemli bir fark bulunamadı (p>0.05). Süt serumu laktoferrin
konsantrasyonları subklinik mastitisli ve kontrol gruplarında sırasıyla 23.98±3.45 μg/ml ve 16.84±1.29 μg/ml olarak
bulundu. Subklinik mastitisli grubun süt serumu laktoferrin düzeylerinin kontrol grubununkine göre istatistiksel olarak
yüksek olduğu belirlendi (p<0.01). Sonuç olarak, koyunlarda süt serumu laktoferrin konsantrasyonunun subklinik mastitisin
teşhisinde faydalı bir belirteç olarak kullanılabileceği kanaatine varıldı.

Anahtar Kelimeler

Etlik Veteriner Mikrobiyoloji Dergisi, Koyun, Laktasyon, Laktoferrin, Subklinik Mastitis, Süt, Cevat NİSBET, Gül Fatma YARIM, Gülay ÇİFTÇİ, Nilgün GÜLTEKİN, Sena ÇENESİZ, Sena ÇENESİZ, 2013

Investigation of Blood and Milk Lactoferrin Concentrations in Lactating Ewes With Subclinical Mastitis

Öz

The aim of the present study was to determine the availability of blood and milk lactoferrin concentrations
in the diagnosis of subclinical mastitis in lactating Karayaka ewes. The milk samples obtained from 43 mid-lactating
Karayaka ewes, aged between 2 and 3, were examined by California Mastitis Test (CMT) and somatic cell count (SCC)
methods. Animals (n=23) had positive CMT score and SCC between 300.000-1.000.000 cells/ml were defined as subclinical
mastitis group, whereas ewes with CMT negative and SCC between 100.000-200.000 cells/ml were described
as control (healthy) group. The serum were obtained from blood and milk samples of ewes and ELISA was performed to
detect blood and milk lactoferrin concentrations. The mean blood lactoferrin concentrations in subclinical mastitis group
and control group were 0.90±0.08 μg/ml and 0.85±0.08 μg/ml, respectively, however the difference was not significant
(p>0.05). The mean milk lactoferrin concentrations in ewes with subclinical mastitis and healthy ewes was 23.98±3.45
μg/ml and 16.84±1.29 μg/ml, respectively (p<0.01). Consequently, it is suggested that the concentration of milk lactoferrin
might be a useful marker in the diagnosis of ewes with subclinical mastitis.

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