Araştırma Makalesi
BibTex RIS Kaynak Göster

Laktasyon Periyodundaki Subklinik Mastitisli Koyunlarda Kkan ve Süt Laktoferrin Konsantrasyonlarının Araştırılması

Yıl 2013, Cilt: 24 Sayı: 2, 37 - 40, 01.12.2013

Öz

Bu çalışmada, laktasyon periyodundaki subklinik mastitisli ve sağlıklı Karayaka ırkı koyunlarda süt ve kan serumlarında
laktoferrin konsantrasyonlarının belirlenmesi amaçlandı. Araştırmada, ortalama 2-3 yaşlarında ve laktasyonlarının
orta döneminde bulunan Karayaka ırkı koyunlar, Kaliforniya mastitis test ve sütte somatik hücre sayımı
yöntemleri ile subklinik mastitis yönünden incelendi. Sütleri Kaliforniya mastitis test pozitif ve somatik hücre sayımı
300.000-1.000.000 olan 23 adet koyun subklinik mastitis grubunu, Kaliforniya mastitis test negatif ve somatik hücre
sayımı <300.000 olan 20 adet koyun ise kontrol grubunu oluşturdu. İneklerden kan ve süt örnekleri alınarak serumları
çıkarıldı. Koyunların kan ve süt serumlarında laktoferrin ölçümleri ELISA test kitleri kullanılarak gerçekleştirildi.
Subklinik mastitisli ve sağlıklı koyunların serum laktoferrin değişimleri sırasıyla ile 0.90±0.08 μg/ml ve 0.85±0.08 μg/
ml olarak tespit edildi ve gruplar arasında istatistiksel olarak önemli bir fark bulunamadı (p>0.05). Süt serumu laktoferrin
konsantrasyonları subklinik mastitisli ve kontrol gruplarında sırasıyla 23.98±3.45 μg/ml ve 16.84±1.29 μg/ml olarak
bulundu. Subklinik mastitisli grubun süt serumu laktoferrin düzeylerinin kontrol grubununkine göre istatistiksel olarak
yüksek olduğu belirlendi (p<0.01). Sonuç olarak, koyunlarda süt serumu laktoferrin konsantrasyonunun subklinik mastitisin
teşhisinde faydalı bir belirteç olarak kullanılabileceği kanaatine varıldı.

Kaynakça

  • Adlerova L, Bartoskova B , Faldyna M, (2008). Lactoferrin: a review.Veterinarni Medicina. 53, 457-468.
  • Alacam E, (1989). Diseases of cattle, Veterinary Medicine Teknografik Matbaa, İstanbul, Turkey. 459-482.
  • Al-Majali A, Ismail ZB, Al-Hami Y, Nour Ay, (2007). Lactoferrin concentration in milk from camels (camelus dromedarius) with and without subclinical mastitis. Int J Appl Res Vet Med. 5, 120-124.
  • Avci G, Sel T, (2004). Milk and serum lactoferrin levels in Angora Goats during lactation period. Ankara Üniv Vet Fak Derg, 51, 181-187.
  • Baker EN, (2005). Lactoferrin: a multi-tasking protein par excellence. Cell Mol Life Sci. 62 (22), 2529-2530.
  • Baveye S, Elas E, Mazurier J, Spik G, Legrand D, (1999). Lactoferrin: a multifunctional glycoprotein involved in the modulation of the inflammatory process. Clin. Chem. Lab. Med. 37, 281-286.
  • Bergonier D, Berthelot X, (2003). New advances in epizootiology and control of ewe mastitis. Livest Prod Sci. 79, 1-16.
  • Brock JH, (2002). The physiology of lactoferrin. Biochem Cell Biol. 80, 1-6.
  • Buchta R, (1991) Ovine lactoferrin: isolation from colostrum and characterization. J Dairy Res. 58(2):211-218.
  • Chen Gh, Yin Li, Chiang JH, Jiang ST, (2008). Cloning and expression of antibacterial goat lactoferricin from Escherichia coli AD494(DE3)pLysS expression system. J Food Prot. 71 (12), 2523-2525.
  • Chen PW, Shyu C, Mao FC, (2003). Antibacterial activity of short hydrophobic and basic-rich peptides. Am J Vet Res. 64 (9), 1088-1092.
  • Cheng JB, Wang JQ, Bu DP, Liu GL, Zhang CG, Wei HY, Zhou LY, Wan JZ, (2008). Factors Affecting the Lactoferrin Concentration in Bovine Milk. J Dairy Sci. 91, 3, 970-976.
  • Contreras A, Sierra D, Sanchez A, Corrale JC, Marco JC, Paape MJ, Gonzalo C, (2007). Mastitis in small ruminants. Small Rum Res. 68, 145-153.
  • De Jong G, Van Dijk JP, Van Eijk HG, (1990). The biology of transferrin. Clin Chim Acta. 190, 1-46.
  • Graaf T, Dwinge RH, (1995). Estimation of milk production losses due to subclinical mastitis in dairy cattle in Costa Rica. In proceeding of the third IDF international mastitis seminar, 11, 28 May-1 June, Tel-Aviv, Israel.
  • Hagiwara SI, Kawa K, Anri A, Nagahata H, (2003). Lactoferrin concentrations in milk from normal and subclinical mastitic cows. J Vet Med Sci. 65 (3), 319-323.
  • Kanyshkova TG, Buneva VN, Nevinsky GA, (2001). Lactoferrin and its biological functions. Biochemistry (Moscow). 66, 1-7.
  • Kawai K, Hagiwara S, Anri A, Nagahata H, (1999). Lactoferrin concentration in milk of ovine clinical mastitis. Vet Res Commun. 23, 391-398.
  • Kilicoglu Ç, Alacam E, Izgur H, Akay O, Wiesner HU, (1989). Eutergesundheitskontrolle von Milchkülen im Gebiet von Ankara (Turkei). Dtsch Tierärztl Wschr. 96, 486-488.
  • Lafi SQ, (2006). Use of somatic cell counts and California Mastitis Test results from udder halves milk samples to detect subclinical intramammary infection in Awassi sheep. Small Rum Res. 62, 83-86.
  • Legrand D, Elass E, Carpentier M, Mazurier J, (2005). Lactoferrin: a modulator of immune and inflammatory responses. Cell Mol Life Sci. 62, 2549-2559.
  • Legrand D, Pierce A, Elass E, Carpentier M, Mariller C, Mazurier J, (2008). Lactoferrin structure and functions. Adv Exp Med Biol. 606, 163-94.
  • Leitner G, Chaffer M, Shamay A, Shapiro F, Merinu E, Ezra A, Saran A, Silanikove N, (2004). Changes in milk composition as affected by subclinical mastitis in sheep. J Dairy Sci. 87, 46-52.
  • Levay PF, Viljoen M, (1995). Lactoferrin: a general review. Haematologica. 80, 252-267.
  • Mazurier J, Lhoste JM, Montreuil J, Spik G, (1983). Comparative study of the iron-binding properties of human transferrins. II. Electron paramagnetic resonance of mixed metal complexes of human lactotransferrin. Biochim Biophys Acta. 30, 745 (1), 44-49.
  • Moguilevsky N, Retegui La, Masson PL, (1985). Comparison of human lactoferrins from milk and neutrophilic leucocytes. Relative molecular mass, isoelectric point, iron-binding properties and uptake by the liver. Biochem J. 229 (2), 353-359.
  • Oberg G, Lindmar G, Moberg L, Venge P, (1983). The peroxidase activity and cellular content of granule proteins in PMN during pregnancy. Br J Haematol. 55 (4), 701-708.
  • Rao CR, (1973). Linear Statistical Inference and Its Applications, John&Sons, New York
  • Schalm OW, Carroll Ej, Jain NC, (1971). Bovine Mastitis, Lea and Febiger, Philadelphia. P, 24.
  • Silanikove N, Shapiro F, Leitner G, Merin U, (2005). Subclinical mastitis affects the plasmin system, milk composition and curd yield in sheep and goats: comparative aspects. In: Hogeveen, H. (Ed.), Mastitis in Dairy Production. Wageningen Academic Press Publishers, The Netherlands, 511-516.
  • Sordillo LM, Streicher KL, (2002). Mammary Gland Immunity and Mastitis Susceptibility. Journal of Mammary Gland Biology and Neoplasia, 7, 2, 135-146.
  • Teng CT, Beard C, Gladwell W, (2002). Differential expression and estrogen response of lactoferrin gene in the female reproductive tract of mouse, rat and hamster. Biol Reprod. 67, 1439-1449.
  • Tripathi BN, (2000). Diseases of the mammary glands of goats and sheep. Vet Bul. 70, 1117-1142.
  • Valenti P, Antonini G, (2005). Lactoferrin: an important host defense against microbial and viral attack. Cell Mol Life Sci. 62, 2576-2587.
  • Vorland LH, (1999). Lactoferrin: a multifunctional glycoprotein. APMIS. 107, 971-981.
  • Weinberg ED, (2003). The therapeutic potential of lactoferrin. Expert Opin Investig Drugs. 12 (5), 841-51.
  • Welty FK, Smith KL, Schanbacher FL, (1976). Lactoferrin concentration during involution of the bovine mammary gland. J Dairy Sci. 59, 224-231.

Investigation of Blood and Milk Lactoferrin Concentrations in Lactating Ewes With Subclinical Mastitis

Yıl 2013, Cilt: 24 Sayı: 2, 37 - 40, 01.12.2013

Öz

The aim of the present study was to determine the availability of blood and milk lactoferrin concentrations
in the diagnosis of subclinical mastitis in lactating Karayaka ewes. The milk samples obtained from 43 mid-lactating
Karayaka ewes, aged between 2 and 3, were examined by California Mastitis Test (CMT) and somatic cell count (SCC)
methods. Animals (n=23) had positive CMT score and SCC between 300.000-1.000.000 cells/ml were defined as subclinical
mastitis group, whereas ewes with CMT negative and SCC between 100.000-200.000 cells/ml were described
as control (healthy) group. The serum were obtained from blood and milk samples of ewes and ELISA was performed to
detect blood and milk lactoferrin concentrations. The mean blood lactoferrin concentrations in subclinical mastitis group
and control group were 0.90±0.08 μg/ml and 0.85±0.08 μg/ml, respectively, however the difference was not significant
(p>0.05). The mean milk lactoferrin concentrations in ewes with subclinical mastitis and healthy ewes was 23.98±3.45
μg/ml and 16.84±1.29 μg/ml, respectively (p<0.01). Consequently, it is suggested that the concentration of milk lactoferrin
might be a useful marker in the diagnosis of ewes with subclinical mastitis.

Kaynakça

  • Adlerova L, Bartoskova B , Faldyna M, (2008). Lactoferrin: a review.Veterinarni Medicina. 53, 457-468.
  • Alacam E, (1989). Diseases of cattle, Veterinary Medicine Teknografik Matbaa, İstanbul, Turkey. 459-482.
  • Al-Majali A, Ismail ZB, Al-Hami Y, Nour Ay, (2007). Lactoferrin concentration in milk from camels (camelus dromedarius) with and without subclinical mastitis. Int J Appl Res Vet Med. 5, 120-124.
  • Avci G, Sel T, (2004). Milk and serum lactoferrin levels in Angora Goats during lactation period. Ankara Üniv Vet Fak Derg, 51, 181-187.
  • Baker EN, (2005). Lactoferrin: a multi-tasking protein par excellence. Cell Mol Life Sci. 62 (22), 2529-2530.
  • Baveye S, Elas E, Mazurier J, Spik G, Legrand D, (1999). Lactoferrin: a multifunctional glycoprotein involved in the modulation of the inflammatory process. Clin. Chem. Lab. Med. 37, 281-286.
  • Bergonier D, Berthelot X, (2003). New advances in epizootiology and control of ewe mastitis. Livest Prod Sci. 79, 1-16.
  • Brock JH, (2002). The physiology of lactoferrin. Biochem Cell Biol. 80, 1-6.
  • Buchta R, (1991) Ovine lactoferrin: isolation from colostrum and characterization. J Dairy Res. 58(2):211-218.
  • Chen Gh, Yin Li, Chiang JH, Jiang ST, (2008). Cloning and expression of antibacterial goat lactoferricin from Escherichia coli AD494(DE3)pLysS expression system. J Food Prot. 71 (12), 2523-2525.
  • Chen PW, Shyu C, Mao FC, (2003). Antibacterial activity of short hydrophobic and basic-rich peptides. Am J Vet Res. 64 (9), 1088-1092.
  • Cheng JB, Wang JQ, Bu DP, Liu GL, Zhang CG, Wei HY, Zhou LY, Wan JZ, (2008). Factors Affecting the Lactoferrin Concentration in Bovine Milk. J Dairy Sci. 91, 3, 970-976.
  • Contreras A, Sierra D, Sanchez A, Corrale JC, Marco JC, Paape MJ, Gonzalo C, (2007). Mastitis in small ruminants. Small Rum Res. 68, 145-153.
  • De Jong G, Van Dijk JP, Van Eijk HG, (1990). The biology of transferrin. Clin Chim Acta. 190, 1-46.
  • Graaf T, Dwinge RH, (1995). Estimation of milk production losses due to subclinical mastitis in dairy cattle in Costa Rica. In proceeding of the third IDF international mastitis seminar, 11, 28 May-1 June, Tel-Aviv, Israel.
  • Hagiwara SI, Kawa K, Anri A, Nagahata H, (2003). Lactoferrin concentrations in milk from normal and subclinical mastitic cows. J Vet Med Sci. 65 (3), 319-323.
  • Kanyshkova TG, Buneva VN, Nevinsky GA, (2001). Lactoferrin and its biological functions. Biochemistry (Moscow). 66, 1-7.
  • Kawai K, Hagiwara S, Anri A, Nagahata H, (1999). Lactoferrin concentration in milk of ovine clinical mastitis. Vet Res Commun. 23, 391-398.
  • Kilicoglu Ç, Alacam E, Izgur H, Akay O, Wiesner HU, (1989). Eutergesundheitskontrolle von Milchkülen im Gebiet von Ankara (Turkei). Dtsch Tierärztl Wschr. 96, 486-488.
  • Lafi SQ, (2006). Use of somatic cell counts and California Mastitis Test results from udder halves milk samples to detect subclinical intramammary infection in Awassi sheep. Small Rum Res. 62, 83-86.
  • Legrand D, Elass E, Carpentier M, Mazurier J, (2005). Lactoferrin: a modulator of immune and inflammatory responses. Cell Mol Life Sci. 62, 2549-2559.
  • Legrand D, Pierce A, Elass E, Carpentier M, Mariller C, Mazurier J, (2008). Lactoferrin structure and functions. Adv Exp Med Biol. 606, 163-94.
  • Leitner G, Chaffer M, Shamay A, Shapiro F, Merinu E, Ezra A, Saran A, Silanikove N, (2004). Changes in milk composition as affected by subclinical mastitis in sheep. J Dairy Sci. 87, 46-52.
  • Levay PF, Viljoen M, (1995). Lactoferrin: a general review. Haematologica. 80, 252-267.
  • Mazurier J, Lhoste JM, Montreuil J, Spik G, (1983). Comparative study of the iron-binding properties of human transferrins. II. Electron paramagnetic resonance of mixed metal complexes of human lactotransferrin. Biochim Biophys Acta. 30, 745 (1), 44-49.
  • Moguilevsky N, Retegui La, Masson PL, (1985). Comparison of human lactoferrins from milk and neutrophilic leucocytes. Relative molecular mass, isoelectric point, iron-binding properties and uptake by the liver. Biochem J. 229 (2), 353-359.
  • Oberg G, Lindmar G, Moberg L, Venge P, (1983). The peroxidase activity and cellular content of granule proteins in PMN during pregnancy. Br J Haematol. 55 (4), 701-708.
  • Rao CR, (1973). Linear Statistical Inference and Its Applications, John&Sons, New York
  • Schalm OW, Carroll Ej, Jain NC, (1971). Bovine Mastitis, Lea and Febiger, Philadelphia. P, 24.
  • Silanikove N, Shapiro F, Leitner G, Merin U, (2005). Subclinical mastitis affects the plasmin system, milk composition and curd yield in sheep and goats: comparative aspects. In: Hogeveen, H. (Ed.), Mastitis in Dairy Production. Wageningen Academic Press Publishers, The Netherlands, 511-516.
  • Sordillo LM, Streicher KL, (2002). Mammary Gland Immunity and Mastitis Susceptibility. Journal of Mammary Gland Biology and Neoplasia, 7, 2, 135-146.
  • Teng CT, Beard C, Gladwell W, (2002). Differential expression and estrogen response of lactoferrin gene in the female reproductive tract of mouse, rat and hamster. Biol Reprod. 67, 1439-1449.
  • Tripathi BN, (2000). Diseases of the mammary glands of goats and sheep. Vet Bul. 70, 1117-1142.
  • Valenti P, Antonini G, (2005). Lactoferrin: an important host defense against microbial and viral attack. Cell Mol Life Sci. 62, 2576-2587.
  • Vorland LH, (1999). Lactoferrin: a multifunctional glycoprotein. APMIS. 107, 971-981.
  • Weinberg ED, (2003). The therapeutic potential of lactoferrin. Expert Opin Investig Drugs. 12 (5), 841-51.
  • Welty FK, Smith KL, Schanbacher FL, (1976). Lactoferrin concentration during involution of the bovine mammary gland. J Dairy Sci. 59, 224-231.
Toplam 37 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Cerrahi
Bölüm Araştırma Makaleleri
Yazarlar

Cevat Nisbet Bu kişi benim

Gül Fatma Yarım Bu kişi benim

Gülay Çiftci Bu kişi benim

Nilgün Gültiken Bu kişi benim

Sena Çenesiz Bu kişi benim

Yayımlanma Tarihi 1 Aralık 2013
Gönderilme Tarihi 1 Aralık 2013
Yayımlandığı Sayı Yıl 2013 Cilt: 24 Sayı: 2

Kaynak Göster

APA Nisbet, C., Yarım, G. F., Çiftci, G., Gültiken, N., vd. (2013). Laktasyon Periyodundaki Subklinik Mastitisli Koyunlarda Kkan ve Süt Laktoferrin Konsantrasyonlarının Araştırılması. Etlik Veteriner Mikrobiyoloji Dergisi, 24(2), 37-40.


15430