Real-Time PCR Tekniği ile Çeşitli Et Ürünlerinde Tavuk ve Sığır Eti Oranlarının Kantitatif Tyini

Yıl 2013, Cilt: 24 Sayı: 2, 41 - 48, 01.12.2013

Yıldız Ayaz Naim Deniz Ayaz Mihriban Aksoy Yusuf Ziya Kaplan

Öz

Et ürünleri üretiminde kullanılan et türlerinin tür bazında ve oransal olarak doğru belirlenmesi tüketicinin aldatılmasının
önlenmesi, halk sağlığının korunması ve üreticiler arası haksız rekabetin önlenmesi açısından büyük önem
taşımaktadır.Yapılan bu çalışmada, hem tür analizi hem de kantitatif analiz yapabildiği üretici firma tarafından ifade
edilen SureFood Animal QUANT Beef ve SureFood Animal QUANT Chicken real- time PCR kitleri kullanılmştır.
Çalışmada deneysel olarak belirli oranlarda (%10 sığır eti + %90 tavuk eti, %20 sığır eti + %80 tavuk eti, %30 sığır eti +
%70 tavuk eti, %50 sığır eti + %50 tavuk eti, %20 tavuk eti + %80 sığır eti, %30 tavuk eti + %70 sığır eti olmak üzere)
sığır eti + tavuk eti karışımlarının yanı sıra farklı oranlarda sığır ve tavuk etinden hazırlanmış et ürünleri (salam, sosis ve
sucuk) test materyali olarak kullanılmıştır. Et karışımlarından ve et ürünlerinden DNA ekstraksiyon işlemleri SureFood
Prep Animal DNA ekstraksiyon kiti (Congen S 1003, Berlin, Almanya) ile yapılmıştır. DNA ekstraksiyonunu takiben
Real-Time PCR tekniği ile et oranları tayin edilmeye çalışılmıştır. PCR analizleri Light Cycler-2 (Roche) cihazında gerçekleştirilmiştir.
Çalışma sonuçlarına göre, kullanılan SureFood Animal QUANT Beef and SureFood Animal QUANT
Chicken real-time PCR kitlerinin deneysel olarak farklı oranlarda sığır ve tavuk etinden hazırlanmış et karışımları ile
yine çeşitli sığır ve tavuk eti karışımlarından yapılan salam, sosis ve sucuk örneklerinde sığır ve tavuk eti oranlarını
kantitatif olarak doğru tespit edemediği ortaya konulmuştur.

Anahtar Kelimeler

Etlik Veteriner Mikrobiyoloji Dergisi, Et Ürünleri, Et Türleri, Kantitatif Analiz, Real-Time PCR, Yıldız AYAZ, Naim Deniz AYAZ, Mihriban AKSOY, Yusuf Ziya KAPLAN, 2013

Quantitative Detection of Chicken and Beef Meat From Meat Products by Real-Time PCR

Öz

Quantitative detection of meat species that are used in production of meat products has a great importance
for preventing consumers from adulteration, protection of public health and prevention of unfair competition between
producers. In this study, SureFood Animal QUANT Beef and SureFood Animal QUANT Chicken real-time PCR kits
were used to detect meat species quantitatively. For this purpose, various proportions (10% bovine + 90% chicken, 20%
bovine + 80% chicken, 30% bovine + 70% chicken, 50% bovine + 50% chicken, 80% bovine + 20% chicken, 70% bovine
+ 30% chicken) of bovine and chicken meat mixtures and meat products (salami, sausage and sucuk from different
bovine + chicken meat) were experimentally prepared. After DNA extraction process of these meat mixtures and products,
Real-Time PCR technique was used for the quantitative detection of meat species. SureFood Prep Animal DNA
extraction kit (Congen S 1003, Berlin, Germany) was used for the extraction of DNA from mixtures. PCR analyses were
carried out with Light Cycler-2 (Roche, Germany). According to the real-time PCR results, SureFood Animal QUANT
Beef and SureFood Animal QUANT Chicken kits were not able to detect the rates of meat species in meat mixtures (beef
and chicken) and meat products (salami, sausage and sucuk) accurately.

Kaynakça

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