The Diagnosis of Bacterial Kidney Disease (BKD) From Cultured Rainbow Trout (Oncorhynchus Mykiss)

Yıl 2008, Cilt: 19 Sayı: 1, 1 - 8, 01.06.2008

Sibel Özkök Selahattin Şen Hikmet Ün

Öz

In this project, totally 360 samples from 5 rainbow trout farms on Kesikköprü barrage lake and aseptically 120 kidney
tissues about 6-12 months rainbow trout from one rainbow trout farm from 2005 to 2007 were collected for isolation and identification
of R.salmoninarum;. Ovarian (coelemic) fluids were collected from 120 mature females of fish farming on Safranbolu.
KDM-2 medium was used for isolation from samples. R.salmoninarum was not isolated on totally 600 samples. DFAT and nested-
PCR results of all samples were negative.
As a result Bacterial Kindey Disease was not detected in Ankara and Safranbolu. In our laboratories, Bacterial Kindey Disease
could be diagnosed rapidly by conventional isolation and identification with DFAT and nested-PCR on rainbow trout.

Kültürü Yapılan Gökkuşağı Alabalıklarında (Oncorhynchus Mykiss) Bakteriyel Böbrek Hastalığı (BKD)’nın teşhisi

Öz

Bu projede Renibacterium salmoninarum izolasyon ve identifikasyonu için, 2005-2007 yılları arasında, Kesikköprü Baraj
Göleti’nde yer alan 5 adet alabalık işletmesinden toplam 360 adet; Safranbolu’da yer alan 1 adet alabalık işletmesinden 120 adet 6-12
aylık alabalıklardan böbrek örnekleri aseptik koşullarda alındı. Safranbolu’da yer alan 1 adet alabalık işletmesinde 120 adet olgun dişi
balıklardan ovaryum sıvısı uygun koşullarda alındı. Toplanan örneklerden izolasyon için KDM-2 medium kullanıldı. Toplam 600
adet örnekten hiç R.salmoninarum izolasyonu olmadı. Aynı örneklerden DFAT ve nested-PCR testleri yapıldı. Örneklerin hiçbirinde
etkene rastlanılamadı.
Sonuç olarak Ankara ve Safranbolu’da Bakteriyel Böbrek Hastalığı saptanamamıştır. Laboratuarımızda alabalıklarda konvansiyonel
izolasyon ve identifikasyon yanında DFAT ve nested-PCR gibi testlerle Bakteriyel Böbrek Hastalığı kısa sürede teşhis edilebilir
hale gelmiştir.

Anahtar Kelimeler

Etlik Veterinerlik Mikrobiyoloji Dergisi, Kültür, Gökkuşağı Balıkları, Bakteri, Böbrek, Sibel ÖZKÖK, Selahattin ŞEN, Hikmet ÜN, 2008

Kaynakça

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