Sığırların Nodüler Ekzantemi hastalığında vektör kene ile konak arasındaki ilişkinin moleküler araştırılması

Yıl 2023, Cilt: 34 Sayı: 1, 11 - 15, 20.06.2023

Hamza Kadı , Cüneyt Tamer , Emre Ozan , Mitat Kurt , Selma Kaya , Abdullah Cavunt , Harun Albayrak

https://doi.org/10.35864/evmd.1244360

Cited By: 1

Öz

Poxviridae familyasının capripoxvirus cinsine ait olan Sığırların Nudüler Ekzantemi Hastalığı Virusu (SNEHV), özellikle Afrika ve Ortadoğu ülkelerinde sığırlarda önemli enfeksiyonlara neden olmaktadır. SNEH, birincil enfeksiyon kaynağı olan kan emici sineklerle yayılan arboviral bir hastalıktır. Ancak deneysel çalışmalarda kenelerde SNHEV'ye ait nükleik asit saptanmış ve bulaşmada kenelerin de rol oynayabileceği bildirilmiştir. Bu çalışma kapsamında, Türkiye’de Karadeniz Bölgesi’nde yeralan ve SNEH enfeksiyonlarının yoğun olarak tespit edildiği Samsun,Sivas ve Tokat illerinden sığır, koyun ve keçilerden kan ve kene örnekleri toplanarak hastalığın vektör-kene-konak ilişkisinin incelenmesi amaçlanmıştır. Bu amaçla Mart 2016-Ekim 2017 tarihleri arasında kene enfestasyonu olan 88 sığır, 511 koyun ve 108 keçiden kene ve kan örnekleri alındı. Kan örnekleri alınan bu hayvanlardan toplam 2508 kene toplandı ve keneler türlerine göre sınıflandırıldı. Real time PCR ile SNHEV nükleik asitlerinin varlığını ortaya çıkarmak için kan örnekleri ve keneler test edildi ve hem kan örneklerinde hem de kenelerde SNHEV nükleik asitleri tespit edilemedi.

Anahtar Kelimeler

SNEHV, Kene, Vektör, real time PCR

Destekleyen Kurum

TAGEM

Proje Numarası

TAGEM/HSGYAD/16/A02/P02/79

Teşekkür

Projeye desteklerinden dolayı Tarımsal Araştırmalar ve Politikalar Genel Müdürlüğü'ne (TAGEM) teşekkür ederiz.

Molecular investigation of the relationship between vector tick and host in Lumpy Skin Disease

Öz

Lumpy Skin Diseases Virus (LSDV), belonging to the capripoxvirus genus of the Poxviridae family, causes significant infections in cattle, especially in African and Middle East countries. LSD is an arboviral disease that spreads with bloodsucking flies as the primary source of infection. However, in experimental studies, nucleic acid belonging to the LSDV has been detected in ticks, and it has been reported that ticks may also play a role in transmission. Within the scope of this study, we have aimed to investigate the vector tick-host relationship of the disease by collecting blood and tick samples from cattle, sheep, and goats in Samsun, Sivas, and Tokat provinces, where LSD infections are intensely detected in the Black Sea Region in Turkey. For this purpose, ticks and blood samples were collected from 88 cattle, 511 sheep, and 108 goats with tick infestation between March 2016 and October 2017. A total of 2508 ticks were collected from these animals, whose blood samples with EDTA were taken, and the ticks were classified according to species. Blood samples and ticks were tested to reveal the presence of LSDV nucleic acids by real-time PCR, and LSDV nucleic acids could not be detected in both blood samples or ticks.

Anahtar Kelimeler

LSDV, Tick, Vector, real-time PCR, SNEHV, Kene, Vektör, real time PCR

Proje Numarası

TAGEM/HSGYAD/16/A02/P02/79

Kaynakça

• Ahmed EM, Eltarabilli M, Shahein MA, Fawzy M. (2021). Lumpy skin disease outbreaks investigation in Egyptian cattle and buffaloes: Serological evidence and molecular characterization of genome termini. Comparative immunology, microbiology, and infectious diseases, 76, 101639.
• Albayrak H, Ozan E, Kadi H, Cavunt A, Tamer C, Tutuncu, M. (2018). Molecular detection and seasonal distribution of lumpy skin disease virus in cattle breeds in Turkey. Med. Weter. 2018, 74 (3), 175-178.
• Bowden TR, Babiuk SL, Parkyn GR, Copps JS, Boyle DB. (2008). Capripoxvirus tissue tropism and shedding: A quantitative study in experimentally infected sheep and goats. Virology, 371(2), 380–393.
• Chihota CM, Rennie LF, Kitching RP, Mellor PS. (2001). Mechanical transmission of lumpy skin disease virus by Aedes aegypti (Diptera: Culicidae). Epidemiology and infection, 126(2), 317–321.
• Coetzer JAW. (2004). Lumpy Skin Disease. In: Infectious Disease of Livestock, Coetzer, J. A. W. And TUSTIN, R. C, Infectious Diseases of Livestock, 2nd ed, 1268-1276. University Press Southern Africa, Oxford Dantas-Torres F, Otranto D, Vatansever Z. (2017). Rhipicephalus turanicus Pomerantzev, 1940. In: Ticks of Europe and North Africa. p. 329–33
• Du toit RM, Weiss KE. (1960). Onderstepoort Veterinary Institute, South Africa. Unpublished observations Green HF. (1959). Lumpy skin disease: its effect on hides and leather and comparison in this respect with some other skin diseases. Bull. Epizoot. Dis. Afr. 7, 63
• Keskin A, Bursali A, Kumlutas Y, Ilgaz C, Tekin S. (2013). Parasitism of immature stages of Haemaphysalis sulcata (Acari: Ixodidae) on some reptiles in Turkey. The Journal of parasitology, 99(5), 752–755. Kitching RP, Mellor PS. (1986). Insect transmission of Capripoxvirus. Research in veterinary science, 40(2), 255–258.
• Lubinga JC, Tuppurainen ES, Stoltsz WH, Ebersohn K, Coetzer JA, Venter EH. (2013a). Detection of lumpy skin disease virus in saliva of ticks fed on lumpy skin disease virus-infected cattle. Experimental & applied acarology, 61(1), 129–138.
• Lubinga JC, Tuppurainen ES, Coetzer JA, Stoltsz WH, Venter EH. (2013b). Transovarial passage and transmission of LSDV by Amblyomma hebraeum, Rhipicephalus appendiculatus and Rhipicephalus decoloratus. Experimental & applied acarology, 62(1), 67–75.
• Lubinga JC, Clift SJ, Tuppurainen ES, Stoltsz WH, Babiuk S, Coetzer JA, Venter EH. (2014). Demonstration of lumpy skin disease virus infection in Amblyomma hebraeum and Rhipicephalus appendiculatus ticks using immunohistochemistry. Ticks and tick-borne diseases, 5(2), 113–120.
• Lubinga JC, Tuppurainen ES, Mahlare R, Coetzer JA, Stoltsz WH, Venter EH. (2015). Evidence of transstadial and mechanical transmission of lumpy skin disease virus by Amblyomma hebraeum ticks. Transboundary and emerging diseases, 62(2), 174–182.
• Menasherow S, Rubinstein-Giuni M, Kovtunenko A, EyngornY, Fridgut O, Rotenberg D, Khinich Y, Stram Y. (2014). Development of an assay to differentiate between virulent and vaccine strains of lumpy skin disease virus (LSDV). Journal of virological methods, 199, 95–101.
• OIE Terrestrial Manuel (2021). Chapter 3. 4. 12. Lumpy Skin Disease Orynbayev MB, Nissanova RK, Khairullin BM, Issimov A, Zakarya K D, Sultankulova KT, Kutumbetov LB, Tulendibayev AB, Myrzakhmetova BS, Burashev ED, Nurabayev SS, Chervyakova OV, Nakhanov AK, Kock RA. (2021). Lumpy skin disease in Kazakhstan. Tropical animal health and production, 53(1), 166. Pfäffle MP, Santos-Silva MM, Jaenson TGT, Vatansever Z, Petney TN. (2017). Haemaphysalis punctata Canestrini and Fanzago, 1878. In: Ticks of Europe and North Africa. p. 237-42
• Rouby S, Hussein KH, Aboelhadid SM, el-Sherif AM. (2017). Role of Rhipicephalus Annulatus Tick in Transmission of Lumpy Skin Disease Virus in Naturally Infected Cattle in Egypt. Santos-Silva MM. and Vatansever Z. (2017). Hyalomma marginatum Koch, 1844. In: Ticks of Europe and North Africa. p. 349–54
• Sevik M, Dogan M. (2017). Epidemiological and Molecular Studies on Lumpy Skin Disease Outbreaks in Turkey during 2014-2015. Transboundary and emerging diseases, 64(4), 1268–1279. Stanko M, Derdáková M, Špitalská E, Kazimírová M. (2021). Ticks and their epidemiological role in Slovakia: from the past till present. Biologia, 1–36. Advance online publication.
• Tuppurainen ES, Stoltsz WH, Troskie M, Wallace DB, Oura CA, Mellor PS, Coetzer JA, Venter EH. (2011). A potential role for ixodid (hard) tick vectors in the transmission of lumpy skin disease virus in cattle. Transboundary and emerging diseases, 58(2), 93–104.
• Tuppurainen ES, Oura CA. (2012). Review: lumpy skin disease: an emerging threat to Europe, the Middle East and Asia. Transboundary and emerging diseases, 59(1), 40–48.
• Tuppurainen ES, Lubinga JC, Stoltsz WH, Troskie M, Carpenter ST, Coetzer JA, Venter EH, Oura CA. (2013a). Mechanical transmission of lumpy skin disease virus by Rhipicephalus appendiculatus male ticks. Epidemiology and infection, 141(2), 425–430.
• Tuppurainen ES, Lubinga JC, Stoltsz WH, Troskie M, Carpenter ST, Coetzer JA, Venter EH, Oura CA. (2013b). Evidence of vertical transmission of lumpy skin disease virus in Rhipicephalus decoloratus ticks. Ticks and tick-borne diseases, 4(4), 329–333.
• Tuppurainen ES, Venter EH, Coetzer JA, Bell-Sakyi L. (2015). Lumpy skin disease: attempted propagation in tick cell lines and presence of viral DNA in field ticks collected from naturally-infected cattle. Ticks and tick-borne diseases, 6(2), 134–140.
• Tuppurainen E, Dietze K, Wolff J, Bergmann H, Beltran-Alcrudo D, Fahrion A, Lamien CE, Busch F, Sauter-Louis C, Conraths FJ, De Clercq K, Hoffmann B, Knauf, S. (2021). Review: Vaccines and Vaccination against Lumpy Skin Disease. Vaccines, 9(10), 1136.
• Vatansever Z. (2017a). Haemaphysalis sulcata Canestrini and Fanzago, 1877. In: Ticks of Europe and North Africa. p. 243–47
• Vatansever Z. (2017b). Rhipicephalus bursa Canestrini and Fanzago, 1878. In: Ticks of Europe and North Africa. p. 299-03
• Weiss KE. (1968). Lumpy Skin Disease Virus. Virol. Monogr. 3, 111-131