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Ege Bölgesi’ndeki sığırlarda paratüberküloz seroprevalansının belirlenmesi

Yıl 2024, Cilt: 35 Sayı: 1, 87 - 90, 04.07.2024
https://doi.org/10.35864/evmd.1470250

Öz

Sığır paratüberkülozu (PTB) sığırların granulomatoz gastroenteritle karakterize kronik seytirli, bulaşıcı, zoonoz bir hastalığıdır. Süt sığırcılığı sektöründe önemli ekonomik kayıplara sebep olmakla birlikte zoonotik karakterli olması insanlarda görülen Chron’s hastalığının temelini oluşturabilme olasılığını düşündürmektedir. Bu çalışmada paratuberküloz hastalığının Ege Bölgesi’nde yetiştirilen süt sığırlarındaki seroprevalansının belirlenmesi amaçlanmıştır. 2022 yılı içerisinde İzmir, Muğla, Denizli, Aydın, Kütahya ve Uşak illerinden tesadüfi örnekleme ile seçilip Bornova Veteriner Kontrol Enstitüsü’ne gönderilen 2 yaşından büyük aşılanmamış süt sığırlarına ait 1000 adet kan serumu üzerinde çalışılmıştır. Bu amaçla diğer tanı yöntemlerinden avantajlı olduğu belirlenen ELISA yöntemi kullanılmıştır. Çalışma materyalinin ELISA ile incelenmesi sonucunda toplamda 84 (%8,4) örnek pozitif; 28 (%2,8) örnek şüpheli bulundu. 888 (%88,8) serum örneği ise negatif olarak tespit edildi. Elde edilen veriler ışığında serumlarda seropozitifliğin görülmesi hastalığın bölgede halen varlığını koruduğunu, koruma, kontrol ve eradikasyon yöntemlerinin belirlenerek uygulanmasının gerekli olduğunu düşündürmektedir.

Etik Beyan

Makalenin etik izin gerektirmediğine dair beyan formu önceki adım ekinde sunulmuştur.

Destekleyen Kurum

İstanbul Üniversitesi- Cerrahpaşa, Veteriner Fakültesi ; Bornova Veteriner Kontrol Enstitüsü Müdürlüğü

Kaynakça

  • Abdellrazeq GS, El-Naggar MM, Khaliel SA and Gamal-Eldin AE. (2014) Detection of Mycobacterium avium subsp. paratuberculosis from cattle and buffaloes in Egypt using traditional culture, serological and molecular based methods. Veterinary World, 7(8).
  • Ayele WY, Macháčková M and Pavlik I. (2001) The transmission and impact of paratuberculosis infection in domestic and wild ruminants. Veterinarni Medicina. 46(7-8), 205-224.
  • Ayele WY, Svastova P, Roubal P, Bartos M and Pavlik I. (2005) Mycobacterium avium subspecies paratuberculosis cultured from locally and commercially pasteurized cow's milk in the Czech Republic. Applied and environmental microbiology. 71(3), 1210-1214.
  • Beard PM, Daniels MJ, Henderson D, Pirie A, Rudge K, Buxton D, Rhind S, Greig A, Hutchings MR, McKendrick I, Stevenson K, Sharp JM. (2001) Paratuberculosis Infection of Nonruminant Wildlife in Scotland. J Clin Microbiol. 39.
  • Berberoğlu M. (2016) Manisa yöresi süt siğirlarinda subklinik paratüberkülozun seroprevalansi. Yüksek Lisans Tezi, Afyon Kocatepe Üniversitesi Sağlık Bilimleri Enstitüsü, Afyon.
  • Boelaert F. et al. (2000) Prevalence of paratuberculosis (Johne’s disease) in the Belgian cattle population. Veterinary Microbiology. 77.3-4 (2000): 269-281.
  • Cetinkaya B, Karahan M, Atil E, Kalin R, De Baere T and Vaneechoutte, M. (2002) Identification of Corynebacterium pseudotuberculosis isolates from sheep and goats by PCR. Veterinary microbiology. 88(1), 75-83.
  • Chapter, W. O. A. H. (2022) Paratuberculosis (Johne’s Disease)(version adopted in May 2021). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals.
  • Çelik A ve Tel Y. (2022) Şanlıurfa Bölgesinde Sığırlarda Paratüberküloz Seroprevalansının Belirlenmesi. Harran Üniversitesi Veteriner Fakültesi Dergisi. 11.1,100-105.
  • Diéguez FJ, González AM, Menéndez S, Vilar MJ, Sanjuán ML, Yus E and Arnaiz I. (2009) Evaluation of four commercial serum ELISAs for detection of Mycobacterium avium subsp. paratuberculosis infection in dairy cows. The Veterinary Journal, 180(2), 231-235.
  • Fawzy, A et al. (2018) Genotyping methods and molecular epidemiology of Mycobacterium avium subsp. paratuberculosis (MAP). International journal of veterinary science and medicine. 6.2, 258-264.
  • Gilardoni LR, Paolicchi FA, Mundo SL. (2012) Bovine paratuberculosis: a review of the advantages and disadvantages of different diagnostic tests. Rev Argent Microbiol. 44.3, 201-215.
  • Good M et al. (2009) Prevalence and distribution of paratuberculosis (Johne's disease) in cattle herds in Ireland. Irish Veterinary Journal. 62,1-10.
  • Harris NB and Barletta RG. (2001) Mycobacterium avium subsp. paratuberculosis in veterinary medicine. Clinical microbiology reviews. 14(3), 489-512.
  • Karakaş, B ve Civelek T. (2018) Kırklareli yöresi süt sığırlarında subklinik paratüberkülozun seroprevalansı. Kocatepe Veterinary Journal. 11.2, 160-164.
  • Kalın MÖ, Gümüşsoy KS ve Hızlısoy H. (2019) Kayseri İlinde Satışa Sunulan Sokak Sütlerinde Mycobacterium paratuberculosis Varlığının Konvansiyonel ve Serolojik Yöntemlerle Araştırılması. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 16(3), 190-197.
  • Karatay, M, Akyüz E, Gökçe G. (2020) Ardahan Yöresindeki Sığırlarda Paratüberküloz’un Seroprevalansı. Kocatepe Veterinary Journal. 13.4, 327-331.
  • Liu X et al. The seroprevalence of Mycobacterium avium subspecies paratuberculosis in dairy cattle in Xinjiang, Northwest China. Irish Veterinary Journal. 70, 1-5.
  • Makav, M ve Gokce E. (2013) Kars yöresi sığırlarında subklinik paratuberkülozun seroprevalansı. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 19.5.
  • Manning, EJB. (2001) Mycobacterium avium subspecies paratuberculosis a review of current knowledge. Journal of Zoo and Wildlife Medicine. 32.3,293-304.
  • Mecitoglu Z, Cihan H, Demir G, Temizel EM ve Senturk S. (2012) Evaluation of Faecal Shedding of Acid-fast Mycobacterium avium subsp. paratuberculosis (map) in both Intradermal Johnin Test-and Serologically (Elisa) Map-positive Cattle. Journal of Biological and Environmental Sciences. 6(18).
  • Nielsen SS and Toft N. (2009) A review of prevalences of paratuberculosis in farmed animals in Europe. Preventive veterinary medicine. 88(1), 1-14.
  • Ozturk D, Pehlivanoglu F, Tok AA, Gunlu S, Guldali Y and Turutoglu H. (2010) Seroprevalence of paratuberculosis in the Burdur province (Turkey), in dairy cattle using the enzyme linked immunosorbent assay (ELISA). Israel J Vet Med. 65(2), 53-7.
  • Park, KT et al. (2006) Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea. Journal of Veterinary Science. 7.4,349. Selim A, El-Haig M ve Galila ES. (2013) Direct detection of Mycobacterium avium subsp. paratuberculosis in bovine milk by multiplex Real-time PCR. Biotechnology in animal husbandry. 29(3), 513-525.
  • Selim A, Halim R, Galila E and Hamouda F. (2021) Seroprevalence and associated risk factors for bovine paratuberculosis in dairy cattle. Journal of the Hellenic Veterinary Medical Society. 72(1), 2647-2652.
  • Singh UP, Singh S, Singh R, Karls RK, Quinn FD, Potter ME and Lillard Jr JW. (2007) Influence of Mycobacterium avium subsp. paratuberculosis on colitis development and specific immune responses during disease. Infection and immunity. 75(8), 3722-3728.
  • Strickland SJ. (2005) Effects of seasonal heat stress on the diagnosis of Mycobacterium avium subsp. paratuberculosis in Texas dairy cattle. Doctoral dissertation, Texas A&M University, Texas.
  • Whitlock RH, Wells SJ, Sweeney RW and Van Tiem J. (2000) ELISA and fecal culture for paratuberculosis (Johne’s disease): sensitivity and specificity of each method. Veterinary microbiology. 77(3-4), 387-398.

Determination of paratuberculosis seroprevalence in cattle in the Aegean Region

Yıl 2024, Cilt: 35 Sayı: 1, 87 - 90, 04.07.2024
https://doi.org/10.35864/evmd.1470250

Öz

Bovine paratuberculosis (PTB) is a chronic, infectious, zoonotic disease of cattle characterized by granulomatous gastroenteritis. Although it causes significant economic losses in the dairy cattle industry, its zoonotic character suggests the possibility that it may form the basis of Chron's disease seen in humans. This study aimed to determine the seroprevalence of paratuberculosis disease in dairy cattle raised in the Aegean Region. In 2022, 1000 blood serum samples of unvaccinated dairy cattle older than 2 years old, selected by random sampling from the provinces of Izmir, Muğla, Denizli, Aydın, Kütahya and Uşak, were studied. For this purpose, the ELISA method, which was determined to be advantageous over other diagnostic methods, was used. As a result of examining the study material with ELISA, a total of 84 (8.4%) samples were positive; 28 (2.8%) samples were found suspicious. 888 (88.8%) serum samples were detected as negative. In the light of the data obtained, the presence of seropositivity in serum suggests that the disease still exists in the region and that prevention, control and eradication methods should be determined and implemented.

Kaynakça

  • Abdellrazeq GS, El-Naggar MM, Khaliel SA and Gamal-Eldin AE. (2014) Detection of Mycobacterium avium subsp. paratuberculosis from cattle and buffaloes in Egypt using traditional culture, serological and molecular based methods. Veterinary World, 7(8).
  • Ayele WY, Macháčková M and Pavlik I. (2001) The transmission and impact of paratuberculosis infection in domestic and wild ruminants. Veterinarni Medicina. 46(7-8), 205-224.
  • Ayele WY, Svastova P, Roubal P, Bartos M and Pavlik I. (2005) Mycobacterium avium subspecies paratuberculosis cultured from locally and commercially pasteurized cow's milk in the Czech Republic. Applied and environmental microbiology. 71(3), 1210-1214.
  • Beard PM, Daniels MJ, Henderson D, Pirie A, Rudge K, Buxton D, Rhind S, Greig A, Hutchings MR, McKendrick I, Stevenson K, Sharp JM. (2001) Paratuberculosis Infection of Nonruminant Wildlife in Scotland. J Clin Microbiol. 39.
  • Berberoğlu M. (2016) Manisa yöresi süt siğirlarinda subklinik paratüberkülozun seroprevalansi. Yüksek Lisans Tezi, Afyon Kocatepe Üniversitesi Sağlık Bilimleri Enstitüsü, Afyon.
  • Boelaert F. et al. (2000) Prevalence of paratuberculosis (Johne’s disease) in the Belgian cattle population. Veterinary Microbiology. 77.3-4 (2000): 269-281.
  • Cetinkaya B, Karahan M, Atil E, Kalin R, De Baere T and Vaneechoutte, M. (2002) Identification of Corynebacterium pseudotuberculosis isolates from sheep and goats by PCR. Veterinary microbiology. 88(1), 75-83.
  • Chapter, W. O. A. H. (2022) Paratuberculosis (Johne’s Disease)(version adopted in May 2021). Manual of Diagnostic Tests and Vaccines for Terrestrial Animals.
  • Çelik A ve Tel Y. (2022) Şanlıurfa Bölgesinde Sığırlarda Paratüberküloz Seroprevalansının Belirlenmesi. Harran Üniversitesi Veteriner Fakültesi Dergisi. 11.1,100-105.
  • Diéguez FJ, González AM, Menéndez S, Vilar MJ, Sanjuán ML, Yus E and Arnaiz I. (2009) Evaluation of four commercial serum ELISAs for detection of Mycobacterium avium subsp. paratuberculosis infection in dairy cows. The Veterinary Journal, 180(2), 231-235.
  • Fawzy, A et al. (2018) Genotyping methods and molecular epidemiology of Mycobacterium avium subsp. paratuberculosis (MAP). International journal of veterinary science and medicine. 6.2, 258-264.
  • Gilardoni LR, Paolicchi FA, Mundo SL. (2012) Bovine paratuberculosis: a review of the advantages and disadvantages of different diagnostic tests. Rev Argent Microbiol. 44.3, 201-215.
  • Good M et al. (2009) Prevalence and distribution of paratuberculosis (Johne's disease) in cattle herds in Ireland. Irish Veterinary Journal. 62,1-10.
  • Harris NB and Barletta RG. (2001) Mycobacterium avium subsp. paratuberculosis in veterinary medicine. Clinical microbiology reviews. 14(3), 489-512.
  • Karakaş, B ve Civelek T. (2018) Kırklareli yöresi süt sığırlarında subklinik paratüberkülozun seroprevalansı. Kocatepe Veterinary Journal. 11.2, 160-164.
  • Kalın MÖ, Gümüşsoy KS ve Hızlısoy H. (2019) Kayseri İlinde Satışa Sunulan Sokak Sütlerinde Mycobacterium paratuberculosis Varlığının Konvansiyonel ve Serolojik Yöntemlerle Araştırılması. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 16(3), 190-197.
  • Karatay, M, Akyüz E, Gökçe G. (2020) Ardahan Yöresindeki Sığırlarda Paratüberküloz’un Seroprevalansı. Kocatepe Veterinary Journal. 13.4, 327-331.
  • Liu X et al. The seroprevalence of Mycobacterium avium subspecies paratuberculosis in dairy cattle in Xinjiang, Northwest China. Irish Veterinary Journal. 70, 1-5.
  • Makav, M ve Gokce E. (2013) Kars yöresi sığırlarında subklinik paratuberkülozun seroprevalansı. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 19.5.
  • Manning, EJB. (2001) Mycobacterium avium subspecies paratuberculosis a review of current knowledge. Journal of Zoo and Wildlife Medicine. 32.3,293-304.
  • Mecitoglu Z, Cihan H, Demir G, Temizel EM ve Senturk S. (2012) Evaluation of Faecal Shedding of Acid-fast Mycobacterium avium subsp. paratuberculosis (map) in both Intradermal Johnin Test-and Serologically (Elisa) Map-positive Cattle. Journal of Biological and Environmental Sciences. 6(18).
  • Nielsen SS and Toft N. (2009) A review of prevalences of paratuberculosis in farmed animals in Europe. Preventive veterinary medicine. 88(1), 1-14.
  • Ozturk D, Pehlivanoglu F, Tok AA, Gunlu S, Guldali Y and Turutoglu H. (2010) Seroprevalence of paratuberculosis in the Burdur province (Turkey), in dairy cattle using the enzyme linked immunosorbent assay (ELISA). Israel J Vet Med. 65(2), 53-7.
  • Park, KT et al. (2006) Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea. Journal of Veterinary Science. 7.4,349. Selim A, El-Haig M ve Galila ES. (2013) Direct detection of Mycobacterium avium subsp. paratuberculosis in bovine milk by multiplex Real-time PCR. Biotechnology in animal husbandry. 29(3), 513-525.
  • Selim A, Halim R, Galila E and Hamouda F. (2021) Seroprevalence and associated risk factors for bovine paratuberculosis in dairy cattle. Journal of the Hellenic Veterinary Medical Society. 72(1), 2647-2652.
  • Singh UP, Singh S, Singh R, Karls RK, Quinn FD, Potter ME and Lillard Jr JW. (2007) Influence of Mycobacterium avium subsp. paratuberculosis on colitis development and specific immune responses during disease. Infection and immunity. 75(8), 3722-3728.
  • Strickland SJ. (2005) Effects of seasonal heat stress on the diagnosis of Mycobacterium avium subsp. paratuberculosis in Texas dairy cattle. Doctoral dissertation, Texas A&M University, Texas.
  • Whitlock RH, Wells SJ, Sweeney RW and Van Tiem J. (2000) ELISA and fecal culture for paratuberculosis (Johne’s disease): sensitivity and specificity of each method. Veterinary microbiology. 77(3-4), 387-398.
Toplam 28 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Veteriner Mikrobiyolojisi
Bölüm Araştırma Makaleleri
Yazarlar

Hatice Zeynep Kaymaz Kanat 0000-0002-3542-8125

Çağatay Nuhay 0000-0002-1475-3041

Yayımlanma Tarihi 4 Temmuz 2024
Gönderilme Tarihi 18 Nisan 2024
Kabul Tarihi 14 Haziran 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 35 Sayı: 1

Kaynak Göster

APA Kaymaz Kanat, H. Z., & Nuhay, Ç. (2024). Ege Bölgesi’ndeki sığırlarda paratüberküloz seroprevalansının belirlenmesi. Etlik Veteriner Mikrobiyoloji Dergisi, 35(1), 87-90. https://doi.org/10.35864/evmd.1470250


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