Farklı Konsantrasyonlardaki Etil Alkol Çözeltilerinin Bakteriler Üzerindeki Etkisinin Değerlendirilmesi

Öz

Amaç: Bu randomize kontrollü deneysel araştırmada farklı konsantrasyonlardaki etil alkol çözeltilerinin bakteri oluşumu üzerine etkisinin değerlendirilmesi amaçlanmaktadır. Yöntem: Ön işlem olarak hacimce farklı konsantrasyonda alkol çözeltileri hazırlanmıştır. Besiyer olarak mikrobiyolojik Agar kullanılmıştır. Üçüncül işlem olarak steril petri kapları alınmış ve kullanılacak petri kapları kirli el,%10 ,%30 , %60 , %70 ,%80 ve %96’lık etil alkol çözeltileri olarak etiketlenmiştir. Günlük hayatta sık kullanılan araçlarından biri olan laptop (monitör ve klavye) örnek alınacak materyal olarak seçilmiştir. Bulgular: Araştırma sonucunda alkol konsantrasyonu arttıkça petri kaplarının içerisindeki koloni sayılarının göz ile görülür derecede azaldığı gözlemlenmiştir. Sonuç: En düşük ve etkili etil alkol konsantrasyonu %70 olarak saptanmıştır. İleriki araştırmalarda farklı ortamlardan alınan örneklerle farklı alkol konsantrasyonlarında bakteri üremesinin gözlemlenmesinin dezenfektan ve antiseptiklerin etkisi konusunda daha fazla bilgiye ulaşmamızı sağlayacağı düşünülmektedir.

Anahtar Kelimeler

• Etanol
• el antiseptikleri
• Covıd-19
• dezenfektanlar

Evaluation of the Effect of Ethyl Alcohol Solutions in Different Concentrations on Bacteria

Öz

Objective: In this randomized controlled experimental study, it is aimed to evaluate the effect of different concentrations of ethyl alcohol solutions on bacterial growth Material and Methods: Alcohol solutions of different concentrations by volume were prepared as a preliminary process. Microbiological agar was used as the medium. As the third process, sterile Petri dishes were obtained, and the dishes to be used were labelled as dirty hand, ethyl alcohol solutions of 10%, 30%, 60%, 70%, 80%, and 96%. Results: It was observed that the count of colonies in petri dishes decreased visibly as the alcohol concentration increased. Approximately 74% reduction was observed in bacterial colonies between 10% ethyl alcohol to 30% ethyl alcohol. About 51% reduction was observed in bacterial colonies between 30% ethyl alcohol to 60% ethyl alcohol. As seen in the table, as the ethyl alcohol concentration increased, the count of colonies detected decreased and eventually vanished with 70% ethyl alcohol. Conclusion: The lowest and most effective ethyl alcohol concentration was found to be 70%. As a result of the research, we think that observing bacterial growth at different alcohol concentrations with samples taken from different environments in future research will provide us with more data on the effect of disinfectants and antiseptics.

Anahtar Kelimeler

• Ethanol
• hand snitizers
• COVID-19
• disinfectants

Kaynakça

1. Avcı, D. & Otkun, M. (2017). Evalution of antibacterial activities of some antiseptics and disinfectants. THDB. 74(3):211-220. https://doi.org/10.5505/TurkHijyen.2017.75002
2. Bal, S. & Şanlı, N.Ö. (2020). Evaluation of the effectiveness of antibacterial wall paint to enhance the hygienicconditions of the interiors. J Fac Eng Archıt Gaz, 35:1913-1922. https://doi.org/10.17341/gazimmfd.678683.
3. Bellissimo-Rodrigues, F., Soule, H., Gayet-Ageron, A., Martin, Y., & Pittet, D. (2016). Should alcohol-based handrub use be customized to healthcare workers’ hand size?. Infect Control Hosp Epidemiol, https://doi.org/ 37(2):219-21. https://doi.org/10.1017/ice.2015.271
4. Cruz, A.F., Abreu, A.O., Souza, P.A., Deveza, B., Medeiros, C.T., Sousa, V.S., Sabagh,B.P., & Bôas, M.H.S. (2022). Journal of Microbiological Methods, 193.106402.
5. Czeisler, M.E., Williams, A.G.G., Molinari, N.A., Gharpure, R., Li, Y.,Barrett, C.E., Robbins, R., Facer-Childs, E.R., Barger, L.K., Cheizler, C.A., Rajaratnam, S.M.W., & Howard, M.E. (2020). Demographic Characteristics, Experiences, and Beliefs Associated with Hand Hygiene Among Adults During the COVID-19 Pandemic, CDC2020 / 69(41),1485–1491. https://doi.org/10.15585/mmwr.mm6941a3. PMID: 33056951;
6. Elzain, A.M., Elsanousi, S.M., & Ibrahim, M.E.A. (2019). Effectiveness of ethanol and methanol alcohols on different isolatesofstaphylococcusspecies. J Bacteriol Mycol, (4):71-73. https://doi.org/ 10.15406/jbmoa.2019.07.00247
7. Jing, J.L.J., Yi, T.P., Bose, R.J.C., McCarthy, J.R., Tharmalingam, N., & Madheswaran, T. (2020). Hand sanitizers: a review on formulation aspects, adverse effects, and regulations. Int. J. Environ. Res. Public Health, 17(9):3326. https://doi.org/ 10.3390/ijerph17093326
8. Kampf, G., & Hollingworth, A. (2008). Comprehensive bactericidal activity of an ethanol-based hand gel in 15 seconds. Annals of Clinical Microbiology and Antimicrobials, 7(2),1-6. https://doi.org/ 10.1186/1476-0711-7-2.

9. Kampf, G., & Kramer, A. (2004). Epidemiologic background of hand hygiene and evaluation of the most important agents for scrubs and rubs. Clin Microbiol Rev,17(4),863-93. https://doi.org/ 10.1128/CMR.17.4.863-893.2004.
10. Lotfinejad, N., Peters, A., & Pittet, D. (2020). Hand hygiene and the novel coronavirus pandemic: the role of healthcare workers. J Hosp Infect, 105(4):776-777. https://doi.org/ 10.1016/j.jhin.2020.03.017
11. Rivera, J.M., Gupta, S., Ramjee, D., El, Hayek, G.Y., El Amiri N., & Desai, A.N. (2020). The Lanset Digital Health, 2(11): 564-566.
12. Sauerbrei, A. (2020). Bactericidal and virucidal activity of ethanol and povidone-iodine. Microbiologyopen, 9(9):100-1812. https://doi.org/ 10.1002%2Fmbo3.1097
13. Sharafi, S.M., & Nafez, K.E.A. (2021). Environmental disinfection against COVID-19 in different areas of health care facilities:areview. Rev Environ Health, 36(2):193-198. https://doi.org/10.1515/reveh-2020-0075
14. Suen, L.K.P., So, Z.Y.Y., Yeung, S.K.W., Lo, K.Y.K., & Lam, S.C. (2019). Epidemiological investigation on hand hygiene knowledge and behaviour: a cross-sectional study on gender disparity. BMC Public Health, 19,401. https://doi.org/10.1186/s12889-019-6705-5
15. Türkmen, A.S., Ceylan, A., &Topuz, A. (2021). Emerging Adults' Information on COVID-19 and Changes in Their Lives. H.Ü. Journal of Faculty of Health Sciences. 8,2. https://doi.org/ 10.21020/husbfd.841364.
16. Wilkinson, M.A.C., Ormandy, K., Bradley, C.R., Fraise, A.P., & Hines, J. (2017). Dose considerations for alcohol-based hand rubs, Journal of Hospital Infection, 95:175-182. https://doi.org/10.1016/j.jhin.2016.12.023
17. World Health Organization. (2021 November Augst 5). Hand hygiene: why, how and when?. 2009. http://www.who.int/gpsc/5may/Hand_Hygiene_Why_How_and_When_Brochure.pdf.
18. World Health Organization (2021 January 3) Coronavirus Disease (COVID-19) Advice for the Public.https://www.who.int/emergencies/diseases/novel-coronavirus-2019/advice-for-public
19. Yusuf, A.A. (2021). Determination of alcohols in hand sanitisers: Are off-the-shelf hand sanitisers what they claim tobe? S Afr J Sci, 117 (11/12): 1-7. https://doi.org/10.17159/sajs.2021/9328