Çim Alanlarında Kahverengi Yama Hastalığına Neden Olan Rhizoctonia solani’ye Karşı Yerli Bacillus ve Florosan Pseudomonas İzolatlarının Biyokontrol Etkinliğinin Belirlenmesi

Yıl 2019, Cilt: 48 Sayı: 1, 21 - 29, 15.02.2020

Ayşe Aşkın Şenocak Filiz Ünal , Mesut Yıldırır

Öz

Rhizoctonia solani, çok sayıda bitkide hastalık oluşturan tüm dünyada yaygın bir patojen fungustur. Etmen ayrıca çim üzerinde önemli zararlara neden olmaktadır. R. solani anastomosis grupları, çim alanlarında yaprak yanıklığı, geniş yamalar ve kahverengi yama hastalıklarına neden olmaktadır. R. solani’nin neden olduğu kahverengi yama hastalığı, özellikle golf sahalarında ve spor alanlarında ciddi hasara neden olmaktadır. Bu çalışmada, yedi antagonist bakteriyel izolat sera koşullarında çim bitkilerinde Rhizoctonia solani’ye karşı etkileri açısından değerlendirilmiştir. Çalışmada bakteri izolatları, 108 cfu/ml oranında çim tohumlarına bulaştırılmıştır. Çalışma sonuçları, tohuma muamele yapılarak elde edilen çim tohumlarının, muamele edilmeyenlere kıyasla R. solani’ye daha az duyarlı olduğunu göstermiştir. Bakteri inoküle edilen uygulamalarda gözlenen hastalık şiddeti, uygulanmamış olanlara kıyasla anlamlı ölçüde azalmıştır (P < 0.001). En yüksek koruma etkisi 253e izolatında (%91.43) gözlenmiştir. Bunu 187c (%87.62) ve 166fp (% 81.59) izolatları izlemiştir. Bakteri izolatlarının tanımlanması, DNA sekans analizi ile yapılmıştır. Bu izolatlar Bacillus cereus, Bacillus sp., Paenibacillus sp., Pseudomonas putida, Stenotrophomanas rhizophila olarak tanımlanmıştır. Çalışma sonucunda, B. cereus 253e, Bacillus sp. 187c ve P. putida 166fp strainlerinin, çim alanlarında kahverengi yama hastalığının biyolojik kontrolünde kullanılabilecek potansiyel izolatlar olduğu belirlenmiştir.

Anahtar Kelimeler

Rhizoctonia solani, Çim, biyolojik mücadele, bitki gelişiminin teşvik edilmesi

Determination of the Biocontrol Efficiency of Native Bacillus and Fluorescent Pseudomonas Isolates Against Rhizoctonia solani Causing Brown Patch Disease on Turfgrass Areas

Öz

Rhizoctonia solani is a pathogenic fungus found world-wide which attacks a great number of plants. The agent also causes significant damages on turfgrass. R. solani anastomosis groups cause leaf blight, large patch and brown patch diseases on turfgrasses areas. Brown patch disease caused by R. solani causes considerable damage especially in golf courses and sport fields. In this study, seven antagonist bacterial isolates were evaluated for their effect on protect to Rhizoctonia solani on turfgrass in greenhouse conditions. The bacteria were inoculated into seed of turgrass at the rate of 108 cfu/ml. Results from the assay showed that turfgrass from the inoculated treatment have less susceptible to R. solani than the uninoculated treatment. The disease severity observed in the inoculated treatment was significantly suppressed in comparison to the uninoculated treatment (P < 0.001). The highest protection effect was observed on isolate 253e (91.43%). The isolates 187c (87.62%) and 166fp (81.59%) followed it. Identifications of bacterial isolates were performed by DNA sequencing analysis. These isolates were identified as Bacillus cereus, Bacillus sp., Paenibacillus sp., Pseudomonas putida, Stenotrophomanas rhizophila. Consequently the study, It has been detected that B. cereus 253e, Bacillus sp. 187c and P. putida 166fp strains are potential isolates that can be used in the biological control of brown patch disease in turfgrass areas.

Anahtar Kelimeler

Rhizoctonia solani, Turfgrass, biological control, plant growth promoting

Kaynakça

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