Araştırma Makalesi
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Microbiological changes of kefir traditionally produced from different milks according to storage time

Yıl 2025, Cilt: 6 Sayı: 1, 9 - 14, 30.04.2025
https://doi.org/10.51753/flsrt.1561917

Öz

Kefir is a fermented dairy product known for its positive effects on health. It has been reported to have positive effects on gastrointestinal diseases, hypertension, metabolic disorders, and the immune system due to its lactic acid bacteria, yeasts, and various bioactive compounds. The study aimed to investigate the microbiological properties of kefirs produced from different milk types and pasteurization processes. Different types of milk were used in kefir production, such as UHT cow, pasteurized cow, open cow, and pasteurized goat. For each milk, kefir was produced with a 24-hour incubation period followed by microbial analysis on days 1, 7, 14, and 21. The microbial flora was assessed based on total bacterial counts, as well as specific enumeration of Lactobacillus sp., Lactococcus sp., coliform bacteria, molds, and yeasts. The pH levels of the kefir samples were also measured. The analysis showed that pH values decreased with increasing storage time in all kefir types. Especially the Lactobacillus sp. count of kefirs produced from goat’s milk was lower than other milk types and decreased until day 21. It was also observed that the number of coliform bacteria decreased faster in kefir produced with UHT milk, while it was not detected in other kefirs after the 14th day. The study revealed that the microbial structure of kefirs varied significantly according to milk type, pasteurization method, and storage time. Open cow’s milk kefirs produced by traditional methods were found to be richer in probiotic bacteria but at risk of contamination.

Kaynakça

  • Acar, C. (2023). Kahveli kefirin kimyasal ve mikrobiyolojik özelliklerin belirlenmesi, Yüksek Lisans Tezi, (pp. 1-99). Aksaray Üniversitesi, Türkiye.
  • Acik, M., Çakiroğlu, F. P., Altan, M., & Baybo, T. (2020). Alternative source of probiotics for lactose intolerance and vegan individuals: sugary kefir. Food Science and Technology, 40, 523-531.
  • Arslan, S. (2015). A review: chemical, microbiological and nutritional characteristics of kefir. CyTA-Journal of Food, 13(3), 340-345.
  • Aydin, E. (2023). Saccharomyces boulardii kullanarak kefir üretimi ve bazı özelliklerinin incelenmesi, Yüksek Lisans Tezi, (pp. 1-96). Ege Üniversitesi, Türkiye.
  • Bakan, R. (2021). Türk mutfağındaki sütlü tatlıların değerlendirilmesi ve inovasyonu, Yüksek Lisans Tezi, (pp. 1-125). Pamukkale Üniversitesi, Türkiye.
  • Bozkir, E., Yilmaz, B., Sharma, H., Esatbeyoglu, T., & Ozogul, F. (2024). Challenges in water kefir production and limitations in human consumption: A comprehensive review of current knowledge. Heliyon.
  • Buran, I. (2020). Probiyotik ve prebiyotiklerin sinbiyotik kullanımının inekve keçi sütünden üretilen kefirlerin kalite özellikleriüzerine etkisi, Doktora Tezi, (pp. 1-187). Ankara Üniversitesi, Türkiye.
  • Cheng, T., Zhang, T., Zhang, P., He, X., Sadiq, F. A., Li, J., ... & Gao, J. (2024). The complex world of kefir: Structural insights and symbiotic relationships. Comprehensive Reviews in Food Science and Food Safety, 23(4), e13364.
  • da Costa, G. M., de Paula, M. M., Costa, G. N., Esmerino, E. A., Silva, R., de Freitas, M. Q., ... & Pimentel, T. C. (2020). Preferred attribute elicitation methodology compared to conventional descriptive analysis: A study using probiotic yogurt sweetened with xylitol and added with prebiotic components. Journal of Sensory Studies, 35(6), e12602.
  • Ciftci, M., & Oncul, N. (2022). Ticari probiyotik içeceklerin bazı mikrobiyolojik özellikleri. Akademik Ziraat Dergisi, 11(1), 165-178.
  • de Souza, H. F., Monteiro, G. F., Bogáz, L. T., Freire, E. N. S., Pereira, K. N., de Carvalho, M. V., ... & Kamimura, E. S. (2024). Bibliometric analysis of water kefir and milk kefir in probiotic foods from 2013 to 2022: A critical review of recent applications and prospects. Food Research International, 175, 113716.
  • Egea, M. B., Santos, D. C. D., Oliveira Filho, J. G. D., Ores, J. D. C., Takeuchi, K. P., & Lemes, A. C. (2022). A review of nondairy kefir products: their characteristics and potential human health benefits. Critical Reviews in Food Science and Nutrition, 62(6), 1536-1552.
  • Ektik, N. (2022). Klasik (olgunlaştırılmış) beyaz peynir üretiminin farklı aşamalarından laktik asit bakterilerinin izolasyonu, identifikasyonu ve teknolojik özelliklerinin belirlenmesi, Doktora Tezi, (pp. 1-128). Balıkesir Üniversitesi, Türkiye.
  • Gokirmakli, C., & Guzel‐Seydim, Z. B. (2022). Water kefir grains vs. milk kefir grains: Physical, microbial and chemical comparison. Journal of Applied Microbiology, 132(6), 4349-4358.
  • Gulhan, A. (2023). Physicochemical, microbiological and sensory analyses of functional detox juices fermented with water kefir grains. Gıda, 48(4), 715-727.
  • Kalamaki, M. S., Kakagianni, M. N., & Angelidis, A. S. (2024). Shifts in ovine (Ovis aries) bulk-tank milk microbiota as a function of cold-storage temperature and duration. International Dairy Journal, 158, 106032.
  • Li, S., Liu, X., Wang, L., Wang, K., Li, M., Wang, X., ... & Wang, Z. (2024). Innovative beverage creation through symbiotic microbial communities inspired by traditional fermented beverages: Current status, challenges and future directions. Critical Reviews in Food Science and Nutrition, 64(28), 10456-10483.
  • Onat, B., Nicin, R. T., Kanıbol, N. A., Cetin, E., Senturk, D. Z., & Simsek, O. (2025). Influence of Jerusalem artichoke on microbial and chemical characteristics of water kefir production. International Journal of Gastronomy and Food Science, 39, 101104.
  • Putri, Y. D., Setiani, N. A., & Warya, S. (2020). The effect of temperature, incubation and storage time on lactic acid content, pH and viscosity of goat milk kefir. Current Research on Biosciences and Biotechnology, 2(1), 101-104.
  • Saleem, K., Ikram, A., Saeed, F., Afzaal, M., Ateeq, H., Hussain, M., ... & Asif Shah, M. (2023). Nutritional and functional properties of kefir. International Journal of Food Properties, 26(2), 3261-3274.
  • Salik, R. A., Bitim, E., Koprualan, O., Selcuk, E., Altay, O., & Ertekin, F. (2023). Encapsulation of olive leaf extract obtained by ultrasonic-assisted extraction method and use in kefir beverage. The Journal of Food, 48(1), 73-93.
  • Sánchez-Rodríguez, R., Terriente-Palacios, C., García-Olmo, J., Osorio, S., & Rodríguez-Ortega, M. J. (2024). Combined Metabolomic and NIRS Analyses Reveal Biochemical and Metabolite Changes in Goat Milk Kefir under Different Heat Treatments and Fermentation Times. Biomolecules, 14(7), 816.
  • Sulmiyati, S., Said, N. S., Fahrodi, D. U., Malaka, R., & Maruddin, F. (2019). The physicochemical, microbiology, and sensory characteristics of kefir goat milk with different levels of kefir grain. Tropical Animal Science Journal, 42(2), 152-158.
  • Sendogan, G., Akan, E., Yerlikaya, O., Meric, S., & Kinik, O. (2021). Changes in benzoic acid content of goat milk kefir produced using different kefir cultures. Mljekarstvo: časopis za unaprjeđenje proizvodnje i prerade mlijeka, 71(1), 60-68.
  • Tavsanlı, N., Yildiz, S., Caliskan, M., & Aydin, S. (2024). Evaluating the potential effect of microalgae on soymilk vegan kefir in terms of physical, chemical, microbiological properties. Algal Research, 82, 103630.
  • Yilmaz, B., Elibol, E., Shangpliang, H. N. J., Ozogul, F., & Tamang, J. P. (2022). Microbial communities in home-made and commercial kefir and their hypoglycemic properties. Fermentation, 8(11), 590.
  • Yousefvand, A., Huang, X., Zarei, M., & Saris, P. E. J. (2022). Lacticaseibacillus rhamnosus GG survival and quality parameters in kefir produced from kefir grains and natural kefir starter culture. Foods, 11(4), 523.

Microbiological changes of kefir traditionally produced from different milks according to storage time

Yıl 2025, Cilt: 6 Sayı: 1, 9 - 14, 30.04.2025
https://doi.org/10.51753/flsrt.1561917

Öz

Kefir is a fermented dairy product known for its positive effects on health. It has been reported to have positive effects on gastrointestinal diseases, hypertension, metabolic disorders, and the immune system due to its lactic acid bacteria, yeasts, and various bioactive compounds. The study aimed to investigate the microbiological properties of kefirs produced from different milk types and pasteurization processes. Different types of milk were used in kefir production, such as UHT cow, pasteurized cow, open cow, and pasteurized goat. For each milk, kefir was produced with a 24-hour incubation period followed by microbial analysis on days 1, 7, 14, and 21. The microbial flora was assessed based on total bacterial counts, as well as specific enumeration of Lactobacillus sp., Lactococcus sp., coliform bacteria, molds, and yeasts. The pH levels of the kefir samples were also measured. The analysis showed that pH values decreased with increasing storage time in all kefir types. Especially the Lactobacillus sp. count of kefirs produced from goat’s milk was lower than other milk types and decreased until day 21. It was also observed that the number of coliform bacteria decreased faster in kefir produced with UHT milk, while it was not detected in other kefirs after the 14th day. The study revealed that the microbial structure of kefirs varied significantly according to milk type, pasteurization method, and storage time. Open cow’s milk kefirs produced by traditional methods were found to be richer in probiotic bacteria but at risk of contamination.

Kaynakça

  • Acar, C. (2023). Kahveli kefirin kimyasal ve mikrobiyolojik özelliklerin belirlenmesi, Yüksek Lisans Tezi, (pp. 1-99). Aksaray Üniversitesi, Türkiye.
  • Acik, M., Çakiroğlu, F. P., Altan, M., & Baybo, T. (2020). Alternative source of probiotics for lactose intolerance and vegan individuals: sugary kefir. Food Science and Technology, 40, 523-531.
  • Arslan, S. (2015). A review: chemical, microbiological and nutritional characteristics of kefir. CyTA-Journal of Food, 13(3), 340-345.
  • Aydin, E. (2023). Saccharomyces boulardii kullanarak kefir üretimi ve bazı özelliklerinin incelenmesi, Yüksek Lisans Tezi, (pp. 1-96). Ege Üniversitesi, Türkiye.
  • Bakan, R. (2021). Türk mutfağındaki sütlü tatlıların değerlendirilmesi ve inovasyonu, Yüksek Lisans Tezi, (pp. 1-125). Pamukkale Üniversitesi, Türkiye.
  • Bozkir, E., Yilmaz, B., Sharma, H., Esatbeyoglu, T., & Ozogul, F. (2024). Challenges in water kefir production and limitations in human consumption: A comprehensive review of current knowledge. Heliyon.
  • Buran, I. (2020). Probiyotik ve prebiyotiklerin sinbiyotik kullanımının inekve keçi sütünden üretilen kefirlerin kalite özellikleriüzerine etkisi, Doktora Tezi, (pp. 1-187). Ankara Üniversitesi, Türkiye.
  • Cheng, T., Zhang, T., Zhang, P., He, X., Sadiq, F. A., Li, J., ... & Gao, J. (2024). The complex world of kefir: Structural insights and symbiotic relationships. Comprehensive Reviews in Food Science and Food Safety, 23(4), e13364.
  • da Costa, G. M., de Paula, M. M., Costa, G. N., Esmerino, E. A., Silva, R., de Freitas, M. Q., ... & Pimentel, T. C. (2020). Preferred attribute elicitation methodology compared to conventional descriptive analysis: A study using probiotic yogurt sweetened with xylitol and added with prebiotic components. Journal of Sensory Studies, 35(6), e12602.
  • Ciftci, M., & Oncul, N. (2022). Ticari probiyotik içeceklerin bazı mikrobiyolojik özellikleri. Akademik Ziraat Dergisi, 11(1), 165-178.
  • de Souza, H. F., Monteiro, G. F., Bogáz, L. T., Freire, E. N. S., Pereira, K. N., de Carvalho, M. V., ... & Kamimura, E. S. (2024). Bibliometric analysis of water kefir and milk kefir in probiotic foods from 2013 to 2022: A critical review of recent applications and prospects. Food Research International, 175, 113716.
  • Egea, M. B., Santos, D. C. D., Oliveira Filho, J. G. D., Ores, J. D. C., Takeuchi, K. P., & Lemes, A. C. (2022). A review of nondairy kefir products: their characteristics and potential human health benefits. Critical Reviews in Food Science and Nutrition, 62(6), 1536-1552.
  • Ektik, N. (2022). Klasik (olgunlaştırılmış) beyaz peynir üretiminin farklı aşamalarından laktik asit bakterilerinin izolasyonu, identifikasyonu ve teknolojik özelliklerinin belirlenmesi, Doktora Tezi, (pp. 1-128). Balıkesir Üniversitesi, Türkiye.
  • Gokirmakli, C., & Guzel‐Seydim, Z. B. (2022). Water kefir grains vs. milk kefir grains: Physical, microbial and chemical comparison. Journal of Applied Microbiology, 132(6), 4349-4358.
  • Gulhan, A. (2023). Physicochemical, microbiological and sensory analyses of functional detox juices fermented with water kefir grains. Gıda, 48(4), 715-727.
  • Kalamaki, M. S., Kakagianni, M. N., & Angelidis, A. S. (2024). Shifts in ovine (Ovis aries) bulk-tank milk microbiota as a function of cold-storage temperature and duration. International Dairy Journal, 158, 106032.
  • Li, S., Liu, X., Wang, L., Wang, K., Li, M., Wang, X., ... & Wang, Z. (2024). Innovative beverage creation through symbiotic microbial communities inspired by traditional fermented beverages: Current status, challenges and future directions. Critical Reviews in Food Science and Nutrition, 64(28), 10456-10483.
  • Onat, B., Nicin, R. T., Kanıbol, N. A., Cetin, E., Senturk, D. Z., & Simsek, O. (2025). Influence of Jerusalem artichoke on microbial and chemical characteristics of water kefir production. International Journal of Gastronomy and Food Science, 39, 101104.
  • Putri, Y. D., Setiani, N. A., & Warya, S. (2020). The effect of temperature, incubation and storage time on lactic acid content, pH and viscosity of goat milk kefir. Current Research on Biosciences and Biotechnology, 2(1), 101-104.
  • Saleem, K., Ikram, A., Saeed, F., Afzaal, M., Ateeq, H., Hussain, M., ... & Asif Shah, M. (2023). Nutritional and functional properties of kefir. International Journal of Food Properties, 26(2), 3261-3274.
  • Salik, R. A., Bitim, E., Koprualan, O., Selcuk, E., Altay, O., & Ertekin, F. (2023). Encapsulation of olive leaf extract obtained by ultrasonic-assisted extraction method and use in kefir beverage. The Journal of Food, 48(1), 73-93.
  • Sánchez-Rodríguez, R., Terriente-Palacios, C., García-Olmo, J., Osorio, S., & Rodríguez-Ortega, M. J. (2024). Combined Metabolomic and NIRS Analyses Reveal Biochemical and Metabolite Changes in Goat Milk Kefir under Different Heat Treatments and Fermentation Times. Biomolecules, 14(7), 816.
  • Sulmiyati, S., Said, N. S., Fahrodi, D. U., Malaka, R., & Maruddin, F. (2019). The physicochemical, microbiology, and sensory characteristics of kefir goat milk with different levels of kefir grain. Tropical Animal Science Journal, 42(2), 152-158.
  • Sendogan, G., Akan, E., Yerlikaya, O., Meric, S., & Kinik, O. (2021). Changes in benzoic acid content of goat milk kefir produced using different kefir cultures. Mljekarstvo: časopis za unaprjeđenje proizvodnje i prerade mlijeka, 71(1), 60-68.
  • Tavsanlı, N., Yildiz, S., Caliskan, M., & Aydin, S. (2024). Evaluating the potential effect of microalgae on soymilk vegan kefir in terms of physical, chemical, microbiological properties. Algal Research, 82, 103630.
  • Yilmaz, B., Elibol, E., Shangpliang, H. N. J., Ozogul, F., & Tamang, J. P. (2022). Microbial communities in home-made and commercial kefir and their hypoglycemic properties. Fermentation, 8(11), 590.
  • Yousefvand, A., Huang, X., Zarei, M., & Saris, P. E. J. (2022). Lacticaseibacillus rhamnosus GG survival and quality parameters in kefir produced from kefir grains and natural kefir starter culture. Foods, 11(4), 523.
Toplam 27 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Mikro ve Nanosistemler
Bölüm Araştırma Makaleleri
Yazarlar

Hilal Doğan Güney 0000-0003-1770-711X

Özlem Özer Altundağ 0000-0001-7117-6335

Meryem Çolak 0000-0001-9876-935X

Yayımlanma Tarihi 30 Nisan 2025
Gönderilme Tarihi 5 Ekim 2024
Kabul Tarihi 23 Ocak 2025
Yayımlandığı Sayı Yıl 2025 Cilt: 6 Sayı: 1

Kaynak Göster

APA Doğan Güney, H., Özer Altundağ, Ö., & Çolak, M. (2025). Microbiological changes of kefir traditionally produced from different milks according to storage time. Frontiers in Life Sciences and Related Technologies, 6(1), 9-14. https://doi.org/10.51753/flsrt.1561917


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