Preparation, Characterization and Investigation of Drug Release Properties of Aloe Vera Loaded Chitosan/Fibroin Films

Yıl 2025, Cilt: 37 Sayı: 1, 75 - 82, 27.03.2025

Pınar Demir , Hasan Yetik

https://doi.org/10.35234/fumbd.1443205

Öz

In this study, biopolymer-based chitosan/silk fibroin/aloe vera-based films with drug carrier properties were prepared. For this purpose, firstly, fibroin protein from raw silk was purified and separated. Then, aloe vera-doped fibroin/chitosan films were prepared by solution casting method and their surface, structural and thermal behaviors were characterized using SEM, FTIR and TGA techniques. Additionally, the release conditions of the antibiotic added to the prepared blend polymer films were determined. Maximum drug release was observed in FK3 film with 75% chitosan content. It was also determined that polymer films exhibited the highest drug release at pH 2 and that the release decreased as pH increased.

Anahtar Kelimeler

Fibroin, Chitosan, Aloe vera, Drug Release

Proje Numarası

MMY.22.01

Aloe Vera Yüklü Kitosan/Fibroin Filmlerin Hazırlanması, Karakterizasyonu ve İlaç Salım Özelliklerinin Araştırılması

Öz

Bu çalışmada ilaç taşıyıcı özelliğe sahip, biyopolimer esaslı kitosan /ipek fibroin/aloe vera içeren filmler hazırlanmıştır. Bu amaçla öncelikle ham ipekten fibroin proteini saflaştırılarak ayrılmıştır. Daha sonra çözeltiden döküm yöntemiyle aloe vera katkılı fibroin/kitosan filmleri hazırlanmış ve SEM, FTIR ve TGA teknikleriyle hem yüzey, hem yapısal hem de ısıl davranışları karakterize edilmiştir. Ayrıca hazırlanan harman polimer filmlerine katılan antibiyotiğin salım şartları belirlenmiştir. Maksimum ilaç salımı %75 kitosan içeriğine sahip FK3 filminde gözlenmiştir. Ayrıca polimer filmlerinin pH 2 de en yüksek ilaç salımı sergilediği ve pH arttıkça salımın azaldığı belirlenmiştir.

Anahtar Kelimeler

Fibroin, Kitosan, Aloevera, İlaç Salım

Etik Beyan

Bu çalışmanın, özgün bir çalışma olduğunu; çalışmanın hazırlık, veri toplama, analiz ve bilgilerin sunumu olmak üzere tüm aşamalarından bilimsel etik ilke ve kurallarına uygun davrandığımı; bu çalışma kapsamında elde edilmeyen tüm veri ve bilgiler için kaynak gösterdiğimi ve bu kaynaklara kaynakçada yer verdiğimi; kullanılan verilerde herhangi bir değişiklik yapmadığımı kabul ederek etik görev ve sorumluluklara riayet ettiğimi beyan ederim.

Destekleyen Kurum

FIRAT ÜNİVERSİTESİ BİLİMSEL ARAŞTIRMA PROJELERİ KOORDİNASYON BİRİMİ

Proje Numarası

MMY.22.01

Teşekkür

Bu çalışmaya MMY.22.01 kodlu projeyle destek veren Fırat Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi’ne teşekkür ederiz.

Kaynakça

• Xing X, Han Y, Cheng H. Biomedical applications of chitosan/silk fibroin composites: A review. Int J Biol Macromol 2023; 240:124407.
• Kaplan DL, Adams WW, Farmer B, et all. Silk Polymers. Mater Sci and Biotech, ACS Publications 1994; 544:2–16.
• Shao Z, Vollrath F. Surprising strength of silkworm silk. Nature 2002; 418:741.
• Rinaudo M. Chitin and chitosan: properties and applications. Prog Polym Sci 2006; 31(7):603-32.
• Gallo M, Naviglio D, Caruso AA, Ferrara L. Applications of chitosan as a functional food. In Novel approaches of nanotechnology in food. Academic Press, 2016; 425-464.
• Marshall JM. Aloe vera gel: What is the evidence? Pharma Jr. 1990; 24:360–2.
• Iarc Monographs. Aloe vera In: Some Drugs and Herbal Products, International Agency for research on Cancer, Lyon, France, 2016.
• Jeencham R, Sutheerawattananonda M, Rungchang S. Novel daily disposable therapeutic contact lenses based on chitosan and regenerated silk fibroin for the ophthalmic delivery of diclofenac sodium. Drug Deliv 2020; 27 (1) :782-790.
• Zhou Y, Liang K, Zhao S. Photopolymerized maleilated chitosan/methacrylated silk fibroin micro/nanocomposite hydrogels as potential scaffolds for cartilage tissue engineering. Int J Biol Macromol 2018; 108:383-390.
• Shao J, Ding Z, Li L. Improved accumulation of TGF-β by photopolymerized chitosan/silk protein bio-hydrogel matrix to improve differentiation of mesenchymal stem cells in articular cartilage tissue regeneration. J Photochem Photobiol B 2020; 203:111744.
• Zhang H, Li LL, Dai FY, Zhang HH, Ni B, Zhou W, Yang X, Wu YZ. Preparation and characterization of silk fibroin as a biomaterial with potential for drug delivery. J Transl Med 2012; 10:117.
• Mosselhy DA, Ge Y, Gasik M, Nordstrom K. Silica Gentamicin nanohybrids: synthesis and antimicrobial action. Materials 2016; 9(3):170.
• Huang J, Qin J, Zhang P, Chen X, You X, Zhang F, Zuo B, Yao M. Facile preparation of a strong chitosan-silk biocomposite film. Carbohydr Polym 2020; 229: 115515.
• Huang B, Liu M, Zhou C. Chitosan composite hydrogels reinforced with natural clay nanotubes. Carbohydr Polym 2017; 175: 689–698.
• Çalamak S, Erdoğdu C, Özalp M, Ulubayram K. Silk fibroin based antibacterial bionanotextiles as wound dressing materials. Mater Sci Eng C, 2014; 43: 11-20.
• Kweon HY, Um IC, Park YH. Structural and thermal characteristics of Antheraea pernyi silk fibroin/chitosan sa film. Polymer 2001; 42: 6651–6656.
• Iqbal DN, Munir A, Abbas M and et all. Polymeric Membranes of Chitosan/Aloe Vera Gel Fabrication With Enhanced Swelling and Antimicrobial Properties for Biomedical Applications. Dose-Response 2023; 21: 2.
• Fu Z, He C, Li H, Yan C. Chen L, Huang J and Liu,Y. A novel hydrophilic–hydrophobic magnetic interpenetrating polymer networks (IPNs) and its adsorption towards salicylic acid from aqueous solution. J Chem Eng 2015; 279(1): 250-259.
• Chu HC, Chen KM. Reuse of activated sludge biomass: I. The rate processes for the adsorption of basic dye. Process Biochem 2002; 37: 595-600.
• Koetting MC, Peters JT, Steichen SD and Peppas N A. Stimulusresponsive hydrogels: Theory, modern advances, and applications. Mater Sci Eng: R: Reports 2015; 93: 1-49.