Effects of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and fluoride on the microhardness of enamel treated with a bleaching agent: ex vivo

Öz

Objective: The aim of this study was to compare the effects of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and 1.23% acidulated phosphate fluoride (APF; pH 3.5) on the microhardness of enamel treated with a bleaching agent.

Materials and Method: Enamel slices (n=32; 2×4 mm) were obtained from 8 mandibular permanent molar teeth. Specimens were embedded into acrylic resin blocks with the enamel surfaces facing upwards. Vickers microhardness (VHN) values of the specimens were recorded at baseline. The specimens were randomly divided into 4 experimental groups, and the experimental designation was as follows: Group 1: no treatment (control), Group 2: 35% hydrogen peroxide (HP), Group 3: HP + CPP-ACP, Group 4: HP + APF application. After treatments, VHN values were measured and recorded again. Specimens were stored in artificial saliva at 37 °C for 1 week. After 1 week second application was done and VHN of the specimens was registered once more. Data were statistically analyzed with ANOVA and Tukey post hoc tests. Values obtained at baseline, and first and second applications were compared using paired samples t-test (α=0.05).

Results: In inter-group comparisons, no statistically significant difference in the enamel microhardness values was found between the baseline, and first and second applications (p>0.05). In intra-group comparisons, again, no statistically significant difference in the enamel microhardness values was found between the baseline, and first and second applications (p>0.05).

Conclusion: According to the limitations of this study it can be concluded that neither the HP application nor the CPP-ACP or APF application after HP had any significant effect on the enamel microhardness.

Anahtar Kelimeler

• Casein phosphopeptide-amorphous calcium phosphate nanocomplex,dental enamel,hardness tests,hydrogen peroxide

Kazein fosfopeptit-amorf kalsiyum fosfat (CPP-ACP) ve floridin ağartma ajanı uygulanmış mine mikrosertliği üzerine etkisi: ex vivo

Öz

Amaç: Bu çalışmanın amacı, kazein fosfopeptit-amorf kalsiyum fosfat (CPP-ACP) ve %1.23 asitlenmiş fosfat florid (APF; pH 3.5) uygulamasının ağartma ajanı uygulanmış mine mikrosertliği üzerine etkisinin değerlendirilmesi ve karşılaştırılmasıdır.

Gereç ve Yöntem: Sekiz alt daimi molar dişten toplam 32 adet mine kesiti (2×4 mm)  elde edildi. Örnekler, mine yüzeyleri açıkta kalacak şekilde akrilik rezin bloklara gömüldü. Örneklerin başlangıç Vickers mikrosertlik (VHN) değerleri kaydedildi. Örnekler rastgele 4 deney grubuna ayrıldı. Grup 1’de herhangi bir uygulama yapılmadı (kontrol). Grup 2’de %35 hidrojen peroksit (HP), Grup 3’te %35 HP+CPP-ACP, Grup 4’te %35 HP+APF uygulandı. Uygulama sonrasında VHN değerleri ölçüldü ve kaydedildi. Örnekler 1 hafta yapay tükürük içerisinde 37 °C’de inkübatörde bekletildi. Bir hafta sonunda ikinci uygulamalar yapıldı ve örneklerin VHN değerleri tekrar ölçüldü ve kaydedildi. Elde edilen veri ANOVA ve Tukey çoklu karşılaştırma testiyle değerlendirildi. Başlangıç, birinci ve ikinci uygulamalar arasındaki farklar bağımlı değişkenler t-testi kullanılarak karşılaştırıldı (α=0.05).

Bulgular: Gruplar arası karşılaştırmalarda, başlangıç, diğer uygulamalar sonrası elde edilen mine mikrosertlik değerleri arasında istatistiksel olarak anlamlı bir fark gözlenmedi (p>0.05). Grup-içi karşılaştırmalarda, yine, başlangıç ve diğer uygulamalar sonrasındaki mine mikrosertlik değerleri arasında istatistiksel olarak anlamlı bir fark bulunmadı (p>0.05).

Sonuç: Bu çalışma koşullarında, %35 HP uygulaması sonrasında veya %35 HP uygulaması ardından CPP-ACP ve APF uygulaması sonrasında mine yüzey sertliğinin belirgin olarak etkilenmediği söylenebilir.

Anahtar Kelimeler

• Diş minesi,hidrojen peroksid,kazein phosphopeptide amorf kalsiyum fosfat nanocomplex,sağlamlık testleri

Kaynakça

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