Effects of adhesive systems applied under fissure sealants to microleakage and shear bond strength

Öz

OBJECTIVE: The aim of this study was to examine the effect of bonding agents under fissure sealants on bond strength and microleakage.

MATERIALS AND METHOD: A total of 210 freshly extracted noncarious human third molar teeth were used (105 teeth for shear bond strength and 105 teeth for microleakage assessment). The teeth were randomly assigned to 14 groups, with 15 teeth in each group. Fissurit FX was applied to the etched enamel in the control groups. In the experimental groups, the self-etch adhesives: Clearfil SE Bond, G Bond, Clearfil DC Bond and the total-etch adhesives: Prelude, Adper Single Bond and Optibond S were applied and light-cured followed by the subsequent application and light-curing of Fissurit FX. Shear bond strength was tested to failure in a universal testing machine with a 1 mm/min crosshead speed. The shear bond strength data was analyzed by using One Way ANOVA and Tukey HSD tests. Microleakage was evaluated by using a dye penetration method after mechanical loading and thermocycling. The microleakage data was analyzed by using the Kruskal-Wallis and Tamhane tests.

RESULTS: The microleakage of the Adper Single Bond Plus group was significantly less than that of the control group (p<0.05). However, the shear bond strength value of the Adper Single Bond Plus group was significantly less than that of the control group, again. The shear bond strength value of Clearfil SE Bond was significantly greater than that of the control group (p<0.05).

CONCLUSION: Clearfil SE Bond improved the shear bond strength, and Adper Single Bond Plus decreased the microleakage of the fissure sealant. An inverse relationship was found between the microleakage and the shear bond strength performances of Adper Single Bond Plus.

Anahtar Kelimeler

• Dental bonding,dental leakage,pit and fissure sealants,shear strength

Fissür örtücünün altına uygulanan adezivlerin makaslama bağlanma kuvveti ve mikrosızıntıya etkisi

Öz

AMAÇ: Bu çalışmanın amacı fissür örtücünün altına adeziv uygulamanın makaslama bağlanma kuvveti ve mikrosızıntıya olan etksinin incelenmesidir. GEREÇ VE YÖNTEM: Çalışmada 210 adet yeni çekilmiş çürüksüz insan üçüncü molar dişi kullanıldı (105 adet diş bağlanma dayanım testi için ve 105 adet diş mikrosızıntı için). Dişler rastgele 14 gruba ayrıldı (n=15). Kontrol gruplarında asitlenmiş mine yüzeylerine Fissurit FX uygulandı. Diğer gruplarda asitlenmiş mine yüzeylerine kendinden pürüzlendirmeli (self-etch) adezivler Clearfil SE Bond, G Bond ve Clearfil DC Bond ve tam pürüzlendirme (total-etch) ardından uygulanan Prelude, Adper Single Bond ve Optibond S adezivleri uygulandı. Daha sonra Fissurit FX uygulanarak polimerize edildi. Bağlanma dayanım kuvvetinin değerlendirilmesinde üniversal test cihazı kullanıldı. İstatistiksel değerlendirmede tek yönlü varyans analizi ve Tukey çoklu karşılaştırma testi kullanıldı. Mekanik ve termal yaşlandırmadan sonra mikrosızıntı değerlendirmesi için boya penetrasyon yöntemi kullanıldı. Veriler, Kruskal-Wallis ve Tamhane testleri ile istatistiksel analize tabi tutuldu. BULGULAR: Adper Single Bond Plus grubunun mikrosızıntı değeri istatistiksel olarak kontrol grubundan düşük bulundu (p < 0.05). Adper Single Bond grubunun makaslama bağlanma değeri kontrol grubundan istatistiksel olarak, yine düşük bulundu. Buna karşın, Clearfil SE bond grubunun makaslama bağlanma dayanım değeri kontrol grubundan istatistiksel olarak daha yüksek bulundu (p < 0.05). SONUÇ: Clearfil SE Bond, fissür örtücünün makaslama bağlanma dayanımını arttırdı; Adper Single Bond Plus, fissür örtücünün mikrosızıntısını azalttı. Adper Single Bond Plus için bağlanma dayanım kuvveti ve mikrosızıntı arasında ters bir ilişki olduğu görüldü.

Anahtar Kelimeler

• Çukur ve fissür örtücüler, dental sızıntı, diş yapıştırma, kayma mukavemeti

Kaynakça

1. Feldens EG, Feldens CA, de Araujo FB, Souza MA. Invasive technique of pit and fissure sealants in primary molars: a SEM study. J Clin Pediatr Dent 1994;18:187-90.
2. Duangthip D, Lussi A. Effects of fissure cleaning methods, drying agents, and fissure morphology on microleakage and penetration ability of sealants in vitro. Pediatr Dent 2003;25:527-33.
3. Salama FS, Al-Hammad NS. Marginal seal of sealant and compomer materials with and without enameloplasty. Int J Paediatr Dent 2002;12:39-46.
4. El-Housseiny AA, Sharaf AA. Evaluation of fissure sealant applied to topical fluoride treated teeth. J Clin Pediatr Dent 2005;29:215-9.
5. Gordon PH, Nunn JH. Fissure sealants. In: Murray JJ, ed. The Prevention of Oral Disease. 3rded. New York: Oxford University Press; 1996. p.78-94.
6. Barroso JM, Torres CP, Lessa FC, Pecora JD, Palma-Dibb RG, Borsatto MC. Shear bond strength of pit-and-fissure sealants to saliva-contaminated and non contaminated enamel. J Dent Child (Chic) 2005;72:95-9.
7. Tulunoglu O, Bodur H, Uçtaşli M, Alaçam A. The effect of bonding agents on the microleakage and bond strength of sealant in primary teeth. J Oral Rehabil 1999;26:436-41.
8. Van Meerbeek B, De Munck J, Mattar D, Van Landuyt K, Lambrechts P. Microtensile bond strengths of an etch & rinse and self-etch adhesive to enamel and dentin as a function of surface treatment. Oper Dent 2003;28:647-60.

9. Jones EF. A 2-year clinical evaluation of two pit and fissure sealants placed with and without the use of a bonding agent. Quintessence Int 1993;24:131-3.
10. Cehreli ZC, Gungor HC. Quantitative microleakage evaluation of fissure sealants applied with or without a bonding agent: results after four year water storage in vitro. J Adhes Dent 2008;10:379-84.
11. Gomes-Silva JM, Torres CP, Contente MM, Oliveira MA, Palma-Dibb RG, Borsatto MC. Bond strength of a pit-and-fissure sealant associated to etch-and-rinse and self-etching adhesive systems to saliva-contaminated enamel: individual vs. simultaneous light curing. Braz Dent J 2008;19:341-7.
12. Feigal RJ, Quelhas I. Clinical trial of a self-etching adhesive for sealant application: success at 24 months with Prompt L-Pop. Am J Dent 2003;16:249-51.
13. Venker DJ, Kuthy RA, Qian F, Kanellis MJ. Twelve-month sealant retention in a school-based program using a self-etching primer/adhesive. J Public Health Dent 2004;64:191-7.
14. Perdigao J, Carmo AR, Anauate-Netto C, Amore R, Lewgoy HR, Cordeiro HJ, et al. Clinical performance of a self-etching adhesive at 18 months. Am J Dent 2005;18:135-40.
15. Grande RH, Ballester R, Singer Jda M, Santos JF. Microleakage of a universal adhesive used as a fissure sealant. Am J Dent 1998;11:109-13.
16. Waggoner WF, Siegal M. Pit and fissure sealant application: updating the technique. J Am Dent Assoc 1996;127:351-61.
17. Mickenautsch S, Mount G, Yengopal V. Therapeutic effect of glassionomers: an overview of evidence. Aust Dent J 2011;56:10-5.
18. Kern M, Thompson VP. Influence of prolonged thermal cycling and water storage on the tensile bond strength of composite to NiCr alloy. Dent Mater 1994;10:19-25.
19. Pinar A, Sepet E, Aren G, Bölükbaşi N, Ulukapi H, Turan N. Clinical performance of sealants with and without a bonding agent. Quintessence Int 2005;36:355-60.
20. Boksman L, McConnell RJ, Carson B, McCutcheon-Jones EF. A 2-year clinical evaluation of two pit and fissure sealants placed with and without the use of a bonding agent. Quintessence Int 1993;24:131-3.
21. Koyuturk AE, Akca T, Yucel AC, Yesilyurt C. Effect of thermal cycling on microleakage of a fissure sealant polymerized with different light sources. Dent Mater J 2006;25:713-8.
22. Burrow MF, Burrow JF, Makinson OF. Pits and fissures: etch resistance in prismless enamel walls. Aust Dent J 2001;46:258-62.
23. Gwinnett AJ. Human prismless enamel and its influence on sealant penetration. Arch Oral Biol 1973;18:441-4.
24. Shimada Y, Tagami J. Effects of regional enamel and prism orientation on resin bonding. Oper Dent 2003;28:20-7.
25. Finger WJ, Fritz U. Laboratory evaluation of one-component enamel/dentin bonding agents. Am J Dent 1996;9:206-10.
26. BarkmeierWW, Los SA, Triolo PT Jr. Bond strengths and SEM evaluation of Clearfil Liner Bond 2. Am J Dent 1995;8:289-93.
27. Kanemura N, Sano H, Tagami J. Tensile bond strength to and SEM evaluation of ground and intact enamel surfaces. J Dent 1999;27:523-30.
28. Al-Sarheed MA. Evaluation of shear bond strength and SEM observation of all-in-one self-etching primer used for bonding of fissure sealants. J Contemp Dent Pract 2006;7:9-16.
29. Dhillon JK, Pathak A. Comparative evaluation of shear bond strength of three pit and fissure sealants using conventional etch or self-etching primer. J Indian Soc Pedod Prev Dent 2012;30:288-92.
30. Feigal RJ, Musherure P, Gillespie B, Levy-Polack M, Quelhas I, Hebling J. Improved sealant retention with bonding agents: a clinical study of two-bottle and single-bottle systems. J Dent Res 2000;79:1850-6.
31. Duangthip D, Lussi A. Microleakage and penetration ability of resin sealant versus bonding system when applied following contamination. Pediatr Dent 2003;25:505-11.
32. Jaffer F, Finer Y, Santerre JP. Interactions between resin monomers and commercial composite resins with human saliva derived esterases. Biomaterials 2002;23:1707-19.
33. Nikaido T, Kunzelmann KH, Ogata M, Harada N, Yamaguchi S, Cox CF, et al. The in vitro dentin bond strengths of two adhesive systems in class I cavities of human molars. J Adhes Dent 2002;4:31-9.
34. Peutzfeldt A, Asmussen E. Determinants of in vitro gap formation of resin composites. J Dent 2004;32:109-15.