HPLC analysis of monomers eluted from self-adhesive resin cements

Yıl 2016, Cilt: 33 Sayı: 3, 109 - 14, 18.08.2016

Özgür Genç Şen Evrim Eligüzeloğlu Dalkılıç Hacer Deniz Arısu Hüma Ömürlü Gürcan Eskitaşçıoğlu

https://doi.org/10.17214/aot.85670

Öz

OBJECTIVE: The aim of this study was to determine the residual monomer leaching from two self-adhesive resin cements polymerized with Light Emitting Diode (LED) or halogen light curing unit.

MATERIALS AND METHOD: Clearfil SA (group A, n = 48) and BisCem (group B, n = 48) cements were inserted in plastic moulds. Each group was further divided into two subgroups. Specimens were light cured with LED light curing unit (LCU) in group A1 and group B1 and halogen LCU in group A2 and group B2 for 20 seconds. The following compounds released from the samples stored in distilled water were analyzed: triethylene glycol-dimethacrylate (TEGDMA) and bisphenol A glycidyl methacrylate (Bis-GMA). Analysis of substances was performed with the use of high performance liquid chromatography, after 1 hour and 24 hour incubation periods. Factorial experimental design and independent t-test was used for statistical analyses.

RESULTS: Self-adhesive resin cements released more Bis-GMA and TEGDMA when they were polymerized with LED LCUs (p<0.05). Difference between different time periods was not statistically significant (p>0.05). Clearfil SA cement released more Bis-GMA than BisCem (p<0.05). BisCem released more TEGDMA than Clearfil SA (p<0.05).

CONCLUSION: The results of this study showed that the quantity of Bis-GMA and TEGDMA leached from self-adhesive resin cements was influenced by the type of LCU and by the type of self-adhesive resin cement.

Anahtar Kelimeler

Dental curing lights, HPLC, resin cements

Kendinden bağlanabilen rezin simanlardan salınan monomerlerin HPLC ile incelenmesi

Öz

AMAÇ: Bu çalışmanın amacı, Light Emitting Diode (LED) veya halojen ışık kaynaklarıyla sertleştirilmiş iki kendinden bağlanabilen rezin simanın artık monomer salımını belirlemektir.

GEREÇ VE YÖNTEM: Clearfil SA (grup A, n = 48) ve BisCem (grup B, n = 48) simanlar plastik kalıplara yerleştirildi. Her grup kendi içinde ikiye ayrıldı. Grup A1 ve B1’deki örnekler LED, grup A2 ve B2’deki örnekler halojen ışık kaynaklarıyla polimerize edildi. Distile suda tutulan örneklerden salınan trietilen glikol-dimetakrilat (TEGDMA) ve bisfenol A glisidilmetakrilat (Bis-GMA) bileşikleri, 1 saat ve 24 saat enkübasyon sonrasında yüksek performanslı likit kromotografisi (HPLC) ile incelendi. İstatistiksel değerlendirmeler için faktöriyel deneysel dizayn ve t testleri kullanıldı.

BULGULAR: Kendinden bağlanabilen rezin simanlar LED ışık kaynaklarıyla sertleştirildiğinde daha fazla Bis-GMA ve TEGDMA monomeri saldılar (p<0.05). Zaman periyodları arasında istatistiksel olarak anlamlı bir fark bulunamadı (p>0.05). Clearfil SA siman BisCem'den daha fazla Bis-GMA saldı (p<0.05); BisCem ise Clearfil SA'dan daha fazla TEGDMA saldı (p<0.05).

SONUÇ: Bu çalışmanın bulguları, kendinden bağlanabilen rezin simanlardan salınan Bis-GMA ve TEGDMA miktarlarının ışık kaynağı ve rezin simanın tipinden etkilendiğini göstermiştir.

Anahtar Kelimeler

Dental polimerize edici ışık kaynakları, HPLC, rezin simanları

Kaynakça

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