Aronya ekstraktı içeren Kolajen/HA kompozit kemik greftinin üretimi ve karakterizasyonu

Öz

Travma, kazalar veya onkolojik rezeksiyonlar sonucu ortaya çıkan kemik doku kayıpları, etkili doku replasmanı için gelişmiş kemik greft ikamelerinin geliştirilmesini gerekli kılmaktadır. Biyouyumluluk ve osteogenezi destekleme gibi temel gerekliliklerin yanı sıra, rezeksiyon bölgesinde kalabilecek mikroskobik tümör hücrelerini baskılayabilecek antikanserojen fonksiyonelliğin kazandırılması, özellikle tümör sonrası rekonstrüksiyonda önemli bir terapötik üstünlük sağlayacaktır. Aronia melanocarpa, güçlü antioksidan, anti-inflamatuvar, antiproliferatif, antimikrobiyal ve antikanser aktiviteleriyle tanınan zengin biyoaktif fitokimyasal içeriğe sahip bir meyvedir. Bu çalışmada, ilk kez aronya ekstraktı kolajen–hidroksiapatit (Kol/HA) esaslı kompozit kemik greftlerinin üretiminde kullanılarak, yeni ve çok fonksiyonlu bir biyomalzeme platformu ortaya konmuştur. Greftler liyofilizasyon tekniğiyle üretilmiş ve genipin ile çapraz bağlanmıştır. Morfolojik, fizikokimyasal ve yapısal özellikleri; taramalı elektron mikroskobu (SEM), X-ışını kırınımı (XRD), ATR-FTIR ve şişme analizleri kullanılarak kapsamlı biçimde karakterize edilmiştir. Ayrıca, L929 fibroblast hücreleri ile gerçekleştirilen MTT analizleri, geliştirilen greftlerin sitotoksik olmadığını ve biyouyumlu yapıda olduğunu ortaya koymuştur.

Anahtar Kelimeler

• Kemik doku mühendisliği
• biyomalzeme
• kolajen
• hidroksiapatit
• aronya

Destekleyen Kurum

İzmir Bakırçay Üniversitesi

Proje Numarası

KBP.2022.010

Etik Beyan

Bu çalışma İzmir Bakırçay Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından KBP.2022.010 numaralı proje kapsamında desteklenmiştir.

Teşekkür

Bu çalışma İzmir Bakırçay Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi tarafından KBP.2022.010 numaralı proje kapsamında desteklenmiştir.

Production and characterization of Aronia extract containing Collagen/HA composite bone graft

Öz

Bone tissue loss resulting from trauma, accidents, or oncological resections necessitates the development of advanced bone graft substitutes for effective tissue replacement. In addition to fundamental requirements such as biocompatibility and the ability to promote osteogenesis, the incorporation of anticarcinogenic functionality capable of suppressing residual microscopic tumor cells within the resection site would provide a substantial therapeutic advantage, particularly in post-tumor reconstruction. Aronia melanocarpa is a rich source of bioactive phytochemicals and has been widely recognized for its potent antioxidant, anti-inflammatory, antiproliferative, antimicrobial, and anticancer activities. In this study, for the first time, Aronia extract was incorporated into the fabrication of collagen–hydroxyapatite (Col/HA)-based composite bone grafts, introducing a novel multifunctional biomaterial platform. The grafts were fabricated via a lyophilization technique and cross-linked with genipin. Their morphological, physicochemical, and structural properties were comprehensively characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), and swelling analyses. Furthermore, cytocompatibility was confirmed by MTT assays using L929 fibroblast cells, demonstrating the non-cytotoxic nature of the developed grafts.

Anahtar Kelimeler

• Bone tissue engineering
• biomaterial
• collagen
• hydroxyapatite
• aronia

Destekleyen Kurum

İzmir Bakırçay University

Proje Numarası

KBP.2022.010

Etik Beyan

This study was supported by the Scientific Research Projects Coordination Unit of İzmir Bakırçay University under project number KBP.2022.010.

Teşekkür

This study was supported by the Scientific Research Projects Coordination Unit of İzmir Bakırçay University under project number KBP.2022.010.

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