Araştırma Makalesi
R.Delemar’ın Üremesi Ve Lipaz Üretimi Üzerine Karıştırma Ve Havalandırma Hızlarının Etkisinin Glukoz Veya Melas Sakkarozu Ve Pfr İçeren Ortamlarda Araştırılması
Öz
Lipazlar (triacylglycerol hydrolases) yağ-su arayüzeyinde uzun zincirli açilgliserollerin ester bağlarının hidrolizini katalizleyen hidrolitik enzimlerdir. Rhizopus delemar’ ın lipaz üretimi için karıştırma hızı, havalandırma hızı ve oksijen taşıyıcı olarak kullanılan perflorokarbon (PFR) miktarının etkisi karbon kaynağı olarak glukoz veya melas sakkarozu kullanılan ortamlarda kesikli bir biyoreaktörde incelenmiştir. Maksimum
lipaz aktiviteleri, ana karbon kaynağı olarak glukoz veya melas sakkarozu içeren ortamlarda sırasıyla 43,0 U/L
ve 103,12 U/L olarak 150 ve 200 devir/min karıştırma hızlarında büyümenin durgun fazında (fermentasyonun
120. saati) elde edilmiştir. Havalandırma hızının lipaz aktivitesi üzerine etkisi 1-4 vvm aralığında incelenmiş, en
yüksek lipaz aktiviteleri karbon kaynağı olarak glukoz veya melas sakkarozu ve %10 PFR içeren ortamlarda 2 vvm havalandırma hızında sırasıyla 102,67 U/L, 171,20 U/L olarak bulunmuştur.
Anahtar Kelimeler
Kaynakça
- Ellaiah P., Prabhakar T., Ramakrishna B., Thaer
- Taleb A., Adinarayana K., “Production of lipase
- by immobilized cells of Aspergillus niger”,
- Process Biochemistry, 39, 525-528, 2004.
- Tweddell R.J., Kermasha S., Combes D., Marty
- A., “Esterification and interesterification activies
- of lipases from Rhizopus niveus and Mucor
- miehei in three different types of organic media: a
- comparative study”, Enzyme Microb. Technol.,
- , 439-445, 1998.
- Isobe K., Akiba T., Yamaguchi S.,
- “Crystallization and characterization of lipase
- from Penicillium cyclopium”, Agric. Biol.
- Chem., 52 (1), 41-47, 1988.
- Farooqui A.A., Yang H.C., Horrock L.A.,
- “Purification of lipases, phospholipases and
- kinases by heparin–Sepharose chromatography”,
- J. Chromatogr., 673 (2), 149 –158, 1994.
- Pabai F., Kermasha S., Morin A., “Lipase from
- Pseudomonas fragi CRDA 323: partial
- purification, characterization and
- interesterification of buffer fat”, Appl.
- Microbiol. Biotechnol., 43, 42-51, 1995.
- Dharmsthiti S., Kuhasuntisuk B., “Lipase from
- Pseudomonas aeruginosa LP602: biochemical
- properties and application for wastewater
- treatment”, J. Indust. Microbiol. Biotechnol.,
- , 75-80, 1998.
- Taipa M.A., Aires-Barros M.R., Cabral J M.S.,
- “Purification of lipases”, J. Biotechnol., 26, 111-
- , 1992.
- Schuepp C., Kermasha S., Michalski M.-C.,
- Morin A., “Production, partial purification and
- characterisation of lipases from Pseudomonas
- fragi CRDA 037”, Process Biochem., 32, 225-
- , 1997.
- Giuseppin M.L.F., “Effects of dissolved oxygen
- concenytration on lipase production by Rhizopus
- delemar”, Appl. Microbiol. Biotechnol., 20,
- -165, 1984.
- Burkert J.F.M., Maugeri F., Rodrigues M.I.,
- “Optimization of extracellular lipase production
- by Geotrichum sp. Using factorial design”,
- Bioresource Technol., 91, 77-84, 2004.
- Vadehra D.A., Harmon L.G. “Factors affecting
- production of staphylococcal lipase”, J. Appl.
- Bacteriol., 32, 147-150, 1969.
- Lee S.Y., Rhee J.S., “Production and partial
- prufication of a lipase from Pseudomonas
- putida”, Enzyme Microbiol. Biotechnol., 15,
- -623, 1993.
- Elibol M., Özer, Da., “Lipase production by
- immobilised Rhizopus arrhizus”, Process
- Biochem., 36, 219-223, 2000.
- Elibol M., Mavituna F., “Effect of
- perfluorodecalin as an oxygen carrier on
- actinorhodin production by Streptomyces
- coelicolor A3(2)”, Appl. Microbiol. Biotechnol.,
- , 206-210, 1995.
- Elibol M., Özer Db., “Influence of oxygen
- transfer on lipase production by Rhizopus
- arrhizus”, Process Biochem., 36, 325-329, 2000.
- Açıkel Ü., Erşan M., Sağ Acıkel Y., “The effects
- of the composition of growth medium and
- fermentation conditions on the production of
- lipase by R. Delemar”, Turk J Biol., 35-44,
- -
- Sağ Acıkel Y., Rhizopus delemar ile lipaz
- üretimi, Proje no: MİSAG 282, TÜBİTAK,
- -
- Wonderwülbecke T., Kieslich K., Erdman H.,
- “Comparison of lipases by different assays”,
- Enzyme and Microbial Technology, 14, 631-
- , 1992.
- Acıkel, U., Ersan, M., “Acid phosphatase
- production by Rhizopus delemar: A role played
- in the Ni(II) bioaccumulation process”, Journal
- of Hazardous Materials, 184, 632-639, 2010.
- Frost GM, Moss DA., “Production of enzymes by
- fermentation”, Biotechnology, Germany:VCH
- Verlagsgessellschaft mbh, 7a, 65–211, 1987.
EFFECTS OF STIRRING AND AERATION RATES ON LIPASE PRODUCTION AND GROWTH OF R. DELEMAR IN THE MEDIA CONTAINING GLUCOSE OR MOLASSES SUCROSE AND PFC
Öz
Anahtar Kelimeler
Kaynakça
- Ellaiah P., Prabhakar T., Ramakrishna B., Thaer
- Taleb A., Adinarayana K., “Production of lipase
- by immobilized cells of Aspergillus niger”,
- Process Biochemistry, 39, 525-528, 2004.
- Tweddell R.J., Kermasha S., Combes D., Marty
- A., “Esterification and interesterification activies
- of lipases from Rhizopus niveus and Mucor
- miehei in three different types of organic media: a
- comparative study”, Enzyme Microb. Technol.,
- , 439-445, 1998.
- Isobe K., Akiba T., Yamaguchi S.,
- “Crystallization and characterization of lipase
- from Penicillium cyclopium”, Agric. Biol.
- Chem., 52 (1), 41-47, 1988.
- Farooqui A.A., Yang H.C., Horrock L.A.,
- “Purification of lipases, phospholipases and
- kinases by heparin–Sepharose chromatography”,
- J. Chromatogr., 673 (2), 149 –158, 1994.
- Pabai F., Kermasha S., Morin A., “Lipase from
- Pseudomonas fragi CRDA 323: partial
- purification, characterization and
- interesterification of buffer fat”, Appl.
- Microbiol. Biotechnol., 43, 42-51, 1995.
- Dharmsthiti S., Kuhasuntisuk B., “Lipase from
- Pseudomonas aeruginosa LP602: biochemical
- properties and application for wastewater
- treatment”, J. Indust. Microbiol. Biotechnol.,
- , 75-80, 1998.
- Taipa M.A., Aires-Barros M.R., Cabral J M.S.,
- “Purification of lipases”, J. Biotechnol., 26, 111-
- , 1992.
- Schuepp C., Kermasha S., Michalski M.-C.,
- Morin A., “Production, partial purification and
- characterisation of lipases from Pseudomonas
- fragi CRDA 037”, Process Biochem., 32, 225-
- , 1997.
- Giuseppin M.L.F., “Effects of dissolved oxygen
- concenytration on lipase production by Rhizopus
- delemar”, Appl. Microbiol. Biotechnol., 20,
- -165, 1984.
- Burkert J.F.M., Maugeri F., Rodrigues M.I.,
- “Optimization of extracellular lipase production
- by Geotrichum sp. Using factorial design”,
- Bioresource Technol., 91, 77-84, 2004.
- Vadehra D.A., Harmon L.G. “Factors affecting
- production of staphylococcal lipase”, J. Appl.
- Bacteriol., 32, 147-150, 1969.
- Lee S.Y., Rhee J.S., “Production and partial
- prufication of a lipase from Pseudomonas
- putida”, Enzyme Microbiol. Biotechnol., 15,
- -623, 1993.
- Elibol M., Özer, Da., “Lipase production by
- immobilised Rhizopus arrhizus”, Process
- Biochem., 36, 219-223, 2000.
- Elibol M., Mavituna F., “Effect of
- perfluorodecalin as an oxygen carrier on
- actinorhodin production by Streptomyces
- coelicolor A3(2)”, Appl. Microbiol. Biotechnol.,
- , 206-210, 1995.
- Elibol M., Özer Db., “Influence of oxygen
- transfer on lipase production by Rhizopus
- arrhizus”, Process Biochem., 36, 325-329, 2000.
- Açıkel Ü., Erşan M., Sağ Acıkel Y., “The effects
- of the composition of growth medium and
- fermentation conditions on the production of
- lipase by R. Delemar”, Turk J Biol., 35-44,
- -
- Sağ Acıkel Y., Rhizopus delemar ile lipaz
- üretimi, Proje no: MİSAG 282, TÜBİTAK,
- -
- Wonderwülbecke T., Kieslich K., Erdman H.,
- “Comparison of lipases by different assays”,
- Enzyme and Microbial Technology, 14, 631-
- , 1992.
- Acıkel, U., Ersan, M., “Acid phosphatase
- production by Rhizopus delemar: A role played
- in the Ni(II) bioaccumulation process”, Journal
- of Hazardous Materials, 184, 632-639, 2010.
- Frost GM, Moss DA., “Production of enzymes by
- fermentation”, Biotechnology, Germany:VCH
- Verlagsgessellschaft mbh, 7a, 65–211, 1987.
Toplam 81 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Konular | Mimarlık |
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 27 Mart 2014 |
| Gönderilme Tarihi | 27 Mart 2014 |
| Yayımlandığı Sayı | Yıl 2013 Cilt: 28 Sayı: 4 |