Nephroprotective Effect of Astaxanthin Against Radiotherapy Via TAS, TOS (Biochemical), TNF-α, CASPASE-3 (Immunohistochemical), SIRT1-P53 (Molecular) Pathway in Rats

Yıl 2023, , 14 - 20, 28.02.2023

Gamze Erkılınç , Mehmet Bedir , Leyla Elif Özgü Ayözger , Hatice Kübra Doğan , Nasıf Fatih Karakuyu

https://doi.org/10.54005/geneltip.1088311

Öz

Aim: To evaluate the effects of Astaxanthin (ATX), known for its antioxidant properties, on the kidneys of rats given radiation by biochemical measuring total oxidant level (TOS), total antioxidant level (TAS), immunohistochemically by Cas3 (Cysteine Aspartate Specific ProteASEs), TNF-α (Tumor necrosis factor-alpha), and molecularly by P53, SIRT (Sirtuin -1) pathways.

Materials and Methods: The rats were divided into 4 groups (8 rats per group): control, radiotherapy (RT), RT+ATX, ATX. ATX was given to rats at 4 mg/kg for 7 days. We evaluated to effect of ATX in rats’ kidneys damaged by RT by comparing all groups with TAS, TOS, Cas 3, TNF-α, and SIRT-1, P53.
Results: TAS levels were similar among the control, RT, RT+ATX, and ATX groups. TOS levels were significantly lower in the ATX group compared to RT, Control, and RT+ATX groups. Histopathologically marked hyperemia and in some kidneys, small hemorrhages were observed in the RT group. In addition, marked glomerular sclerosis was also detected in this group. With ATX, we observed significant improvement in the RT+ATX group. Immunohistochemically revealed increased Cas3 expressions, tubular cells in TNF-α expressions in the RT group. ATX treatment decreased Cas3 and TNF-α expression in the RT+ATX group. No Cas3 and TNF-α expression was observed in both control and ATX groups. There was no significant difference between the groups in SIRT-1, P53 values.
Conclusion: Astaxanthin was observed that it is a carotenoid that may benefit the recovery of tubular and glomerular cells in kidney damage after radiation, and it has positive effects on oxidative stress.

Anahtar Kelimeler

Radiotherapy effects, Rat kidney, Astaxanthin, Caspase 3, SIRT-1, P53, TNF-α

Destekleyen Kurum

This study was supported by the Scientific Research Project Unit of Suleyman Demirel University

Proje Numarası

TSG-2020-8134

Teşekkür

none

Astaksantin’in Sıçanlarda TAS, TOS (Biyokimyasal), TNF-α, CASPASE-3 (İmmünohistokimyasal), SIRT-1-P53 (Moleküler) Yolu Aracılığıyla Radyoterapiye Karşı Nefroprotektif Etkisi

Öz

Amaç: Antioksidan özelliği ile bilinen Astaksantin'in (ATX) radyasyon verilen sıçanların böbrekleri üzerindeki etkilerini biyokimyasal olarak total oksidan düzeyi (TOS), total antioksidan düzeyi (TAS), Cas3 (Sistein Aspartat Spesifik ProteASE'ler), TNF-a (Tümör nekroz faktörü-alfa) ile immünohistokimyasal olarak ve P53, SIRT (Sirtuin -1) yolakları ile moleküler olarak değerlendirmek.
Gereç ve Yöntem: Sıçanlar 4 gruba ayrıldı (grup başına 8 sıçan): kontrol, radyoterapi (RT), RT+ATX, ATX. Sıçanlara 7 gün boyunca 4 mg/kg ATX verildi. RT ile hasar görmüş sıçanların böbreklerinde ATX'in etkisini tüm gruplarda TAS, TOS, Cas 3, TNF-α ve SIRT-1, P53 ile karşılaştırarak değerlendirdik.
Bulgular: TAS düzeyleri kontrol, RT, RT+ATX ve ATX grupları arasında benzerdi. TOS seviyeleri, ATX grubunda RT, Kontrol ve RT+ATX gruplarına kıyasla anlamlı derecede düşüktü. RT grubunda histopatolojik olarak belirgin hiperemi ve bazı böbreklerde küçük kanamalar gözlendi. Ayrıca bu grupta belirgin glomerüler skleroz da saptandı. ATX ile, RT+ATX grubunda belirgin şekilde iyileşme gözlemledik. İmmünohistokimyasal olarak RT grubunda Cas3 ekspresyonlarında artış, TNF-α’nın tübüler hücreler ekspresyonu saptandı. ATX tedavisi, RT+ATX grubunda Cas3 ve TNF-α ekspresyonunu azalttı. Hem kontrol hem de ATX gruplarında Cas3 ve TNF-α ekspresyonu gözlenmedi. SIRT-1, P53 değerlerinde gruplar arasında anlamlı fark yoktu.
Sonuç: Astaksantin'in radyasyon sonrası böbrek hasarında tübüler ve glomerüler hücrelerin iyileşmesine fayda sağlayabilecek bir karotenoid olduğu ve oksidatif stres üzerinde olumlu etkileri olduğu gözlendi.

Anahtar Kelimeler

Radyoterapi etkileri, Rat böbreği, Astaksantin, Kaspaz 3, , Kaspaz 3, SIRT-1, P53, TNF-α

Proje Numarası

TSG-2020-8134

Kaynakça

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