Apigenin-7-Glucoside Attenuates Hydrogen Peroxide-Induced Oxidative Stress and Neuronal Death in SH-SY5Y Cells Via Activation of Antioxidant Enzymes System and Inhibition of Caspases Genes Expression
Öz
Aims: In this study, it was aimed to investigate the possible neuroprotective effect of Apigenin-7-Glycoside (AP7Glu), a natural product with known anticancer and anti-inflammatory activity, against hydrogen proxide (H2O2)-induced oxidative stress in SH-SY5Y cells.
Methods: The effect of H2O2 and AP7Glu on cell viability was determined by XTT analysis. SOD and catalase analyzes were performed to determine antioxidant enzyme activities. Expression levels of antioxidant defense system related genes and caspase genes were evaluated by qRT-PCR analysis.
Results: After treatment of 300 µM H2O2 to SH-SY5Y cells for 24 hours, cell viability was determined as 63.18% and this dose was used to induce oxidative stress. H2O2 treatment in the presence of different concentrations of AP7Glu increased cell viability compared to H2O2-treated cells. And, H2O2 decreased SOD and catalase enzyme activities compared to control group. With the treatment of H2O2 in the presence of AP7Glu, an increase was observed in these enzymes activities compared to H2O2-treated cells. In addition, H2O2 decreased the expression levels of genes associated with antioxidant defense system and increased expression levels of Caspase genes. And, H2O2 treatment in the presence of AP7Glu increased the expression levels of genes associated with antioxidant defense system and decreased CASP8 expression compared to H2O2-treated cells.
Conclusion: AP7Glu has a neuroprotective effect against H2O2-induced oxidative stress and neuronal cell death.
Anahtar Kelimeler
Apigenin-7-Glycoside, Hydrogen peroxide, Oxidative stress, SH-SY5Y, Apigenin-7-Glikozit, Hidrojen peroksit, Oksidatif stres
Kaynakça
- Jenner P. Oxidative stress in Parkinson's disease. Ann Neurol 2003;53 Suppl 3:S26-36
- Smith MA, Rottkamp CA, Nunomura A, Raina AK, Perry G. Oxidative stress in Alzheimer's disease. Biochim Biophys Acta 2000;1502(1):139-144
- Overmyer K, Brosché M, Kangasjärvi J. Reactive oxygen species and hormonal control of cell death. Trends Plant Sci 2003;8(7):335-342
- Crane FL, Low H. Reactive oxygen species generation at the plasma membrane for antibody control. Autoimmun Rev 2008;7(7):518-522
- Bhandary B, Marahatta A, Kim HR, Chae HJ. Involvement of oxidative stress in endoplasmic reticulum stress and its associated diseases. Int J Mol Sci 2012;14(1):434-456
- Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squadrito F, Altavilla D, Bitto A. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev 2017;2017:8416763
- Yavarpour-Bali H, Ghasemi-Kasman M, Pirzadeh M. Curcumin-loaded nanoparticles: a novel therapeutic strategy in the treatment of central nervous system disorders. Int J Nanomedicine 2019;14:4449-4460
- Collin F. Chemical Basis of Reactive Oxygen Species Reactivity and Involvement in Neurodegenerative Diseases. Int J Mol Sci 2019;20(10):2407
- Annunziato L, Amoroso S, Pannaccione A, Cataldi M, Pignataro G, D'Alessio A, Sirabella R, Secondo A, Sibaud L, Di Renzo GF. Apoptosis induced in neuronal cells by oxidative stress: the role played by caspases and intracellular calcium ions. Toxicol Lett 2003;139(2-3):125-133
- Singh A, Kukreti R, Saso L, Kukreti S. Oxidative Stress: A Key Modulator in Neurodegenerative Diseases. Molecules 2019;24(8):1583