Investigation of Cytokines, Biochemical Parameters and Oxidative Stress Levels in Serum of Patients with Acute Pancreatitis

Yıl 2024, , 158 - 165, 30.04.2024

Nihayet Bayraktar , Oruç Aslan , Ahmet Uyanıkoğlu , Ali Öztürk , Mehmet Bayraktar , Hamza Erdoğdu

https://doi.org/10.54005/geneltip.1264527

Öz

Objective: This study aimed to find new serum biochemistry parameters, especially for the early identification of severe AP. In the study serum cytokine levels (TNF-A, IL-1, IL-6, IL-10, IL-21), biochemical parameters (Aquaporin-1, Hepcidine, Iron, Zinc, Copper, Nitric Oxide, C-Reactive protein), and oxidative stress parameters analysis were performed.
Method: A total of 46 patients with AP and 46 healthy controls were included in this study. Serum cytokine levels, biochemical, and oxidative stress parameters by the ELISA method, and CRP by the immunoturbidimetric method were measured.
Results: When patients with AP and control groups were compared, all studied parameters were found to be statistically significant (p<0.05). However, IL-1 was found to be insignificant (p>0.05). Therefore, the Mann-Whitney U test, which is a non-parametric test, was found suitable to determine whether there was a significant difference between the patient and control groups.
Conclusions: Investigating the role of cytokines, oxidative stress and other biochemical parameters in the pathogenesis and course of the disease may contribute to a better understanding of the disease process and its therapeutic value. It showed a significant increase in oxidative stress parameters and biochemical parameters such as aquaporin-1, hepcidin, lipase, and amylase, which may help in the diagnosis of AP.

Anahtar Kelimeler

Acute pancreatitis, cytokine, oxidative stress, some other biochemical parameters

Proje Numarası

19092

Akut Pankreatitli Hastaların Serumlarında Sitokinler, Biyokimyasal Parametreler ve Oksidatif Stres Düzeylerinin Araştırılması

Öz

Amaç: Bu çalışma, özellikle şiddetli AP'nin erken teşhisi için yeni serum biyokimya parametreleri bulmayı amaçladı. Çalışmada serum sitokin seviyeleri (TNF-A, IL-1, IL-6, IL-10, IL-21), biyokimyasal parametreler (Aquaporin-1, Hepsidin, Demir, Çinko, Bakır, Nitrik Oksit, C-Reaktif protein) ve oksidatif stres parametreleri analizi yapıldı.
Yöntem: Bu çalışmaya AP'li toplam 46 hasta ve 46 sağlıklı kontrol dahil edildi. ELISA yöntemi ile serum sitokin düzeyleri, biyokimyasal ve oksidatif stres parametreleri, immünotürbidimetrik yöntemle de CRP ölçüldü.
Bulgular: AP'li hastalar ve kontrol grubu karşılaştırıldığında çalışılan tüm parametreler istatistiksel olarak anlamlı bulundu (p<0.05). Ancak IL-1'in önemsiz olduğu bulundu (p>0.05). Bu nedenle hasta ve kontrol grupları arasında anlamlı fark olup olmadığını belirlemek için parametrik olmayan bir test olan Mann-Whitney U testi uygun bulunmuştur.
Sonuç: Sitokinlerin, oksidatif stresin ve diğer biyokimyasal parametrelerin hastalığın patogenezindeki ve seyrindeki rolünün araştırılması, hastalık sürecinin ve tedavi edici değerinin daha iyi anlaşılmasına katkı sağlayabilir. AP tanısında yardımcı olabilecek aquaporin-1, hepsidin, lipaz ve amilaz gibi oksidatif stres parametrelerinde ve biyokimyasal parametrelerde önemli bir artış gösterdi.

Anahtar Kelimeler

Akut pankreatit, sitokin, oksidatif stres, diğer bazı biyokimyasal parametreler

Destekleyen Kurum

HARRAN ÜNİVERSİTESİ - BİLİMSEL ARAŞTIRMA PROJELERİ BİRİMİ

Proje Numarası

19092

Kaynakça

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