Evaluation of Adhesin Antigen Test Results in Samples Sent with Suspicion of Amebiasis

Yıl 2024, , 542 - 546, 31.08.2024

Duygu Beder , Fatma Esenkaya Taşbent

https://doi.org/10.54005/geneltip.1470640

Öz

Objective: The study aimed to evaluate E.histolytica-specific ELISA adhesin antigen test results in stool samples sent to the medical microbiology laboratory with suspicion of amebiasis.
Materials and Methods: A total of 1120 stool samples were included in the study, which were sent to the medical microbiology laboratory with suspicion of amebiasis in the two-year period between January 2022 and December 2023. The results of ELISA adhesin antigen test studied on stool samples were evaluated retrospectively.
Results: Of the 1120 samples in the study, 578 (51.6%) were obtained from male and 542 (48.4%) from female patients. The mean age of the patients was 33.92±24.33 (0-94) years. There were 335 (30%) samples from pediatric patients, and the mean age was determined as 4.48 ±4.78. The positivity rate of E.histolytica specific ELISA adhesin antigen test was 14.3% (n=161). Of the positive patients, 77 (47.8%) were male and 84 (52.2%) were female. There were 55 (34.1%) positive samples from pediatric patients. While there was no statistically significant difference between the positivity status and age of the patients, or between the positivity status and gender (p>0.05), the seasonal difference was considered significant (p<0.05).
Conclusion: With the E.histolytica-specific ELISA adhesin antigen test, E.histolytica and non-pathogenic E.dispar can be differentiated and patients can be prevented from receiving unnecessary treatment.

Anahtar Kelimeler

amebiasis, Entamoeba histolytica, ELISA adhesin antigen test

Amibiyazis Şüphesiyle Gönderilen Örneklerde Adezin Antijen Test Sonuçlarının Değerlendirilmesi

Öz

Amaç: Bu çalışmada amibiyazis şüphesiyle tıbbi mikrobiyoloji laboratuvarına gönderilen dışkı örneklerinde E. histolytica’ya spesifik ELISA adezin antijen test sonuçlarının değerlendirilmesi amaçlanmıştır.
Gereç ve Yöntem: Ocak 2022 ile Aralık 2023 tarihleri arasındaki iki yıllık dönemde amibiyazis şüphesiyle tıbbi mikrobiyoloji laboratuvarına gönderilen dışkı örneklerinde incelenen ELISA (Cellabs, Entamoeba Celisa Path, Brookvale, NSW Avustralya) adezin antijen testi sonuçları retrospektif olarak değerlendirilmiştir. Olgulara ait test sonuçları, cinsiyet, yaş, tarih ve örneğin geldiği klinik bilgileri hastanemiz laboratuvar bilgi sisteminden elde edilmiştir. İstatistiksel analizlerde Fisher kesin ki-kare testi kullanılmıştır.
Bulgular: Çalışmadaki 1120 örneğin 578 (%51.6)’i erkek, 542 (%48.4)’si kadın hastalara aittir. Hastaların yaşları 0 - 94 yaş aralığında olup, yaş ortalaması 33.92 (standart sapma: ±24.33) olarak bulunmuştur. Çalışmada çocuk hastalara ait 335 (%30) örnek bulunmaktadır ve bu hastaların yaş ortalamaları 4.48 (standart sapma: ±4.78) olarak tespit edilmiştir. E. histolytica’ ya spesifik ELISA adezin antijen testi ile 161 (%14.3) örnek pozitif bulunmuştur. Pozitif örneklerin 77 (% 47.8)’si erkek, 84 (%52.2)’ü kadın hastalara aittir. Pozitif örnekler içinde çocuk hastalara ait 55 (%34.1) örnek bulunmaktadır. Hastaların pozitiflik durumu ile yaşı arasında ve pozitiflik durumu ile cinsiyeti arasında istatistiksel olarak anlamlı bir farklılık görülmemişken (p>0.05), mevsimler arasındaki farklılık anlamlı kabul edilmiştir (p<0.05).
Sonuç: Çalışmamızda E. histolytica’ ya spesifik ELISA adezin antijen testi pozitifliği %14.3 oranında belirlenmiş ve bu oran ülke literatür verilerine yakın bulunmuştur. Amibiyazisin tropikal ve subtropikal iklim özelliklerinde daha sık görüldüğü belirlenmiştir. Ucuz, hızlı ve deneyimli personel gerektirmeyen E. histolytica’ya spesifik ELISA adezin antijen testi kullanımı ile yanlış tanı oranı düşürülerek gereksiz tedavi uygulamaları azaltılabilmektedir.

Anahtar Kelimeler

amibiyazis, Entamoeba histolytica, ELISA adezin antijen testi

Kaynakça

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