Investigation of Bromelain, Inulin and Saccharomyces cerevisiae Effects Against Escherichia coli Infection Developed in Fibroblast Wound Model

Yıl 2025, Cilt: 35 Sayı: 6, 1253 - 1265, 31.12.2025

Sümeyye Başer , Demet Çelebi , Özgür Çelebi , Sıdıka Genç , Ali Taghizadehghalehjoughi

https://doi.org/10.54005/geneltip.1737789

https://izlik.org/JA24AN47RR

Öz

Aim: Wound infections, especially in today's world where antibiotic resistance is increasing, pose a serious health problem. Escherichia coli complicates treatment by forming a biofilm. In this study, the individual and combined effects of bromelain, inulin, and Saccharomyces cerevisiae on an Escherichia coli infection model created in fibroblast cells were investigated.
Methods: The antibacterial activity of bromelain, inulin and Saccharomyces cerevisiae was evaluated using the disk diffusion method; minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and fractional inhibitory concentration (FIC) analyses were conducted. Biofilm inhibitory activity was evaluated using the crystal violet staining method. In vitro wound model has been created using human fibroblast cells. Bromelain, inulin, and Saccharomyces cerevisiae agents were applied to the cells infected with Escherichia coli. Cell viability was determined by the MTT test and analyzed through measurements of total oxidant status (TOS) and total antioxidant capacity (TAC). To evaluate the inflammatory response, IL-1β and IL-10 levels were quantitatively measured using the ELISA method.
Results: According to disk diffusion, MIC/MBC, FIC, and antibiofilm analyses, it has been determined that the triple combination (Bromelain + Inulin + Saccharomyces cerevisiae) provides the highest antibacterial and synergistic effect. In the antibiofilm tests, bromelain showed the strongest inhibitory effect (58.3%), but the effect of the triple combination was more limited. In the MTT analysis, cell viability increased in the treatment groups, reaching 112% especially in the triple combination. While TOS levels increased with infection, they significantly decreased after treatment, whereas TAC levels increased. In ELISA analyses, the IL-1β level significantly increased with infection, but decreased with treatment. IL-10 levels, on the other hand, significantly increased, especially in the triple combination, demonstrating an anti-inflammatory response.
Conclusions: The combination of bromelain, inulin and Saccharomyces cerevisiae has shown antimicrobial, antioxidant, and anti-inflammatory activity against Escherichia coli infection, thereby supporting wound healing.

Anahtar Kelimeler

Bromelain , Escherichia coli , Inulin , Saccharomyces cerevisiae , Wound infection

Bromelain, İnulin ve Saccharomyces cerevisiae'nin Fibroblast Yara Modelinde Gelişen Escherichia coli Enfeksiyonuna Karşı Etkilerinin Araştırılması

https://izlik.org/JA24AN47RR

Öz

Amaç: Yara enfeksiyonları, özellikle antibiyotik direncinin arttığı günümüzde, ciddi bir sağlık sorunu oluşturmaktadır. Escherichia coli, biyofilm oluşturarak tedaviyi zorlaştırmaktadır. Bu çalışmada, fibroblast hücrelerinde oluşturulan Escherichia coli enfeksiyon modelinde bromelain, inulin ve Saccharomyces cerevisiae’nin bireysel ve kombinasyon halindeki etkilerini araştırmaktır.
Gereç ve Yöntemler: Bromelain, inülin ve Saccharomyces cerevisiae’nin antibakteriyel etkinliği, disk difüzyon yöntemiyle değerlendirilmiş; minimum inhibitör konsantrasyon (MİK), minimum bakterisidal konsantrasyon (MBK) ve fraksiyonel inhibitör konsantrasyon (FİK) analizleri yapılmıştır. Biyofilm inhibitör etkinlik, kristal viyole boyama yöntemiyle değerlendirilmiştir. İnsan fibroblast hücreleri ile in vitro yara modeli oluşturulmuştur. Escherichia coli ile enfekte edilen hücrelere bromelain, inülin ve Saccharomyces cerevisiae ajanları halinde uygulanmıştır. Hücre canlılığı MTT testi ile belirlenmiş, toplam oksidan seviye (TOS) ve toplam antioksidan kapasite (TAK) ölçümleriyle analiz edilmiştir. İnflamatuvar yanıtı değerlendirmek amacıyla, IL-1β ve IL-10 düzeyleri ELISA yöntemiyle kantitatif olarak ölçüldü.
Bulgular: Disk difüzyon, MİK/MBK, FİK ve antibiyofilm analizlerine göre, üçlü kombinasyonun (Bromelain + Inulin + Saccharomyces cerevisiae) en yüksek antibakteriyel ve sinerjistik etkiyi sağladığı belirlenmiştir. Antibiyofilm testlerinde, bromelain en güçlü inhibitör etkiyi göstermiştir (%58,3), ancak üçlü kombinasyon etkisi daha sınırlı kalmıştır. MTT analizinde, tedavi gruplarında hücre canlılığı artmış, özellikle üçlü kombinasyonda %112’ye ulaşmıştır. TOS seviyeleri enfeksiyonla artarken, tedavi sonrası anlamlı şekilde azalmış, TAC seviyeleri ise artmıştır. ELISA analizlerinde, IL-1β düzeyi enfeksiyonla belirgin şekilde artmışken, tedaviyle azalmıştır. IL-10 düzeyleri ise özellikle üçlü kombinasyonda önemli ölçüde artarak antiinflamatuvar yanıtı göstermiştir.
Sonuçlar: Bromelain, inulin ve Saccharomyces cerevisiae kombinasyonu, Escherichia coli enfeksiyonuna karşı antimikrobiyal, antioksidan ve antiinflamatuvar etkinlik göstererek yara iyileşmesini desteklemiştir.

Anahtar Kelimeler

Bromelain , Escherichia coli , Inulin , Saccharomyces cerevisiae , Yara enfeksiyonu

Kaynakça

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