Günün Farklı Zamanlarında Yapılan Futbol Maçlarında Oksidan-Antioksidan Dengenin İncelenmesi
Yıl 2017,
Cilt: 27 Sayı: 4, 129 - 135, 01.12.2017
Sermin Algül
Oğuz Özçelik
Öz
Amaç: Total oksidan seviye vücutta oksidatif stres düzeyinin arttığını gösteren ölçütlerden biridir. Total antioksidan seviye; total vücut antioksidan seviyesini göstermektedir. Bu çalışmanın amacı günün farklı zamanlarında yapılan akut egzersizlerin total oksidan ve antioksidan düzeyi üzerine olan etkilerini antrenmanlı ve sedanter deneklerde karşılaştırmalı olarak araştırmaktır. Böylece sirkadiyen ritim ve egzersizin; TAS ve TOS düzeyini nasıl değiştirdiği, antrenmanlı ve sedanter denekler arasında fark olup olmadığı belirlenecektir.Gereç ve Yöntem: Bu çalışmaya katılan 10 antrenmanlı ve 10 sedanter erkek denek sabah ve gece futbol maçı yapmışlardır. Maçtan önce ve maçtan hemen sonra venöz kan örnekleri alınmıştır. Serum total oksidan ve antioksidan düzeyi ELISA yöntemi kullanılarak analiz edilmiştir. Bulgular: Total antioksidandüzeyiantrenmanlıvesedanterdeneklerdesabah 1.03±0.08 mmol/L’den 0.91±0.06 mmol/L’ye; 0.98±0.08 mmol/L’den 0.83±0.1 mmol/L’ye; sırasıyla vegece 0.94±0.1 mmol/L’den 0.82±0.09 mmol/L’ye; 1.11±0.16 mmol/L’den 0.96±0.10 mmol/L’ye; sırasıyla istatistikselolarakanlamlıorandaazalmıştır p < 0.01 . Total oksidandüzeyiantrenmanlıvesedanterdeneklerdesabah 14.96±4.3 µmol/L’den 18.01±8.6 µmol/L’ye; 13.65±2.7 µmol/L’den 18.33±2.9 µmol/L’ye; sırasıyla vegece 13.62±3.2 µmol/L’den 19.33±6.3 µmol/L’ye; 14.83±4.2 µmol/L’den 25.79±10.4 µmol/L’ye; sırasıyla istatistiksel olarak anlamlı oranda artmıştır p < 0.01 . Sonuç: Bu çalışma sonuçları akut egzersizin TAS düzeyini azaltırken TOS düzeyini artırdığını göstermektedir. Bunun sonucunda akut egzersiz sırasında oksidatif stresin arttığı ve egzersiz zamanının önemi açığa çıkmaktadır
Kaynakça
- WHO. Preamble the Constitution of the World Health Or- ganization as adopted by the International Health Confe- rence, New York, 1946.
- Vuori IM, Lavie CJ, Blair SN. Physical Activity Promotion in thehealthcaresystem. Mayo Clin Proc 2013;88:1446-61.
- Hambrecht R, Adams V, Erbs S, et al. Regular physical ac- tivity improves endothelial function in patients with coro- nary artery disease by increasing phosphorylation of endo- thelial nitric oxide synthase. Circulation 2003;107:3152-8.
- Wasserman K, Hansen JE, Sue DY, Stringer W, Whipp BJ. Principles of Exercise Testing and Interpretation: Including PathophysiologyandClinical Applications. 5th ed. Lippin- cott Williams &Wilkins, New York, NY, USA; 2012.
- Wiecek M, Maciejczyk M, Szymura J, Szygula Z. Changes inoxidative stressand acid-base balance in men and women following maximal-intensity physical exercise. Physiol Res 2015;64:93-102.
- Berzosa C, Cebrián I, Fuentes-Broto LJ, et al. Acute exercise increases Plasma total antioxidant status and antioxidant enzyme activities in untrained men. Biomed Biotechnol 2011;2011:540458.
- Teixeira VH, Valente HF, Casal SI, Marques FP, Moreira PA. Blood antioxidant and oxidative stress biomarkers acute- responses to a 1000-m kayak sprint in elite male kayakers. J Sports Med Phys Fitness 2013;53:71-9.
- Zalavras A, Fatouros IG, Deli CK, et al. Age related respon- ses in circulating markers of redox status in healthy adoles- cents and adults during the course of a training macrocycle. Oxid Med Cell Longev 2015;2015:283921.
- Benot S, Molinero P, Soutto M, Goberna R, Guerrero JM. Circadian Variations in therat serum total antioxi- dant status: correlation with melatonin levels. J Pineal Res 1998;25:1-4.
- Vollaard NB, Shearman JP, Cooper CE. Exercise-induced oxidative stress: myths, realities and physiological relevan- ce. Sports Med 2005;12:1045-62.
- Reilly T, Ekblom B. The Use of recovery methods post-exer- cise. J Sports Sci 2005;6:61927.
- Cazzola R, Russo-Volpe S, Cervato G, Cestaro B. Biochemi- cal assessments of oxidative stress, erythrocyte membrane fluidity and antioxidant status in professional soccer player- sand sedentary controls. Eur J ClinInvest 2003;10:924-30.
- Brites FD, Evelson PA, Christiansen MG, et al. Soccer players under regular training Show oxidative stress but an improved plasma antioxidant status. ClinSci (Lond) 1999;4:381-5.
- Banfi G, Malavazos A, Iorio E, et al. Plasma Oxidative Stress Biomarkers, nitricoxideand heat shock protein 70 in trai- ned elite soccerplayers. Eur J ApplPhysiol 2005;5:483-6.
- Demirbag R, Yilmaz R, Guzel S, et al. Effects of treadmill exercise test on oxidative/antioxidative parameters and DNA damage. Anadolu Kardiyol Derg 2006;6:135-40.
- Michailidis Y, Jamurtas AZ, Nikolaidis MG, et al. Sampling time is crucial for measurement of aerobic exercise-induced oxidative stress. MedSci Sports Exerc 2007;39:1107-13.
- Kurban S, Mehmetoglu I, Yerlikaya HF, Gonen S, Erdem S. Effect of chronic regular exercise on serum ischemia-mo- dified albumin levels and oxidative stress in type 2 diabetes mellitus. Endocr Res 2011;36:116-23.
- Ficicilar H, Zergeroglu AM, Ersoz G, et al. The effects of short-term training on platelet function and total antioxi- dant capacity in rats. Physiol Res 2006;55:151-6.
- Ascensão A, Rebelo A, Oliveira E, et al. Biochemical impa- ct of a soccermatch-analysis of oxidative stress and musc- le damage markers throughout recovery. Clin Biochem 2008;41:841-51.
- Benot S, Goberna R, Reiter RJ, et al. Physiological Levels of melatonin contribute the antioxidant capacity of human serum. J Pineal Res 1999;27:59-64.
- Hammouda O, Chtourou H, Chahed H, et al. Diurnal vari- ations of plasma homocysteine, total antioxidant status, and biological markers of muscle injury during repeated sprint: effect on performance an muscle fatigue--a pilot study. Chronobiol Int 2011;28:958-67.
Yıl 2017,
Cilt: 27 Sayı: 4, 129 - 135, 01.12.2017
Sermin Algül
Oğuz Özçelik
Öz
Objective: Total oxidantlevelsare a measurethatshowingincreasedlevels of oxidativestress in body. Total antioxidantlevelsareshowedlevels of total body antioxidantlevels.Aim of thisstudy is investigatedeffects on levels of total oxidantandantioxidantlevelsthatperformeddifferenttimesacuteexercise as comparatively in trainedanduntrainedsubjects. Circadianrhythmandexercisewilldetermine how changing total oxidantandantioxidantlevelsandwhetherdifferencebetweentrainedanduntrainedsubjects. Material and Methods: Ten trained and ten untrained subjects participated this study that performed playing soccer match in morning and night. Pre and post match venous blood samples were taken. Serum total oxidant and antioxidant levels were analysed using Enzyme Linked-Immunosorbent Assay.Results: Total antioxidant levels were decreased significantly in morning from 1.03±0.08 mmol/L to 0.91±0.06 mmol/L; from 0.98±0.08 mmol/L to 0.83±0.1 mmol/L; respectively and night from 0.94±0.1 mmol/L to 0.82±0.09 mmol/L; from 1.11±0.16 mmol/L to 0.96±0.10 mmol/L; respectively in trained and untrained subjects p < 0.01 . Total oxidant levels were increased significantly in morning from 14.96±4.3 µmol/Lto18.01±8.6 µmol/L; from13.65±2.7 µmol/L to 18.33±2.9 µmol/L; respectively and night from 13.62±3.2 µmol/Lto19.33±6.3 µmol/L; from14.83±4.2 µmol/L to 25.79±10.4 µmol/L; respectively in trained and untrained subjects p < 0.01 . Conclusion: Thisstudydocumentsshoweddecreasing total antioxidantlevelsincreasing total oxidantlevelsduringacuteexercise. Thereby, thisresultshavebringoutintotheopenincreasedoxidativestressduringacuteexerciseandimportant of exercisetiming
Kaynakça
- WHO. Preamble the Constitution of the World Health Or- ganization as adopted by the International Health Confe- rence, New York, 1946.
- Vuori IM, Lavie CJ, Blair SN. Physical Activity Promotion in thehealthcaresystem. Mayo Clin Proc 2013;88:1446-61.
- Hambrecht R, Adams V, Erbs S, et al. Regular physical ac- tivity improves endothelial function in patients with coro- nary artery disease by increasing phosphorylation of endo- thelial nitric oxide synthase. Circulation 2003;107:3152-8.
- Wasserman K, Hansen JE, Sue DY, Stringer W, Whipp BJ. Principles of Exercise Testing and Interpretation: Including PathophysiologyandClinical Applications. 5th ed. Lippin- cott Williams &Wilkins, New York, NY, USA; 2012.
- Wiecek M, Maciejczyk M, Szymura J, Szygula Z. Changes inoxidative stressand acid-base balance in men and women following maximal-intensity physical exercise. Physiol Res 2015;64:93-102.
- Berzosa C, Cebrián I, Fuentes-Broto LJ, et al. Acute exercise increases Plasma total antioxidant status and antioxidant enzyme activities in untrained men. Biomed Biotechnol 2011;2011:540458.
- Teixeira VH, Valente HF, Casal SI, Marques FP, Moreira PA. Blood antioxidant and oxidative stress biomarkers acute- responses to a 1000-m kayak sprint in elite male kayakers. J Sports Med Phys Fitness 2013;53:71-9.
- Zalavras A, Fatouros IG, Deli CK, et al. Age related respon- ses in circulating markers of redox status in healthy adoles- cents and adults during the course of a training macrocycle. Oxid Med Cell Longev 2015;2015:283921.
- Benot S, Molinero P, Soutto M, Goberna R, Guerrero JM. Circadian Variations in therat serum total antioxi- dant status: correlation with melatonin levels. J Pineal Res 1998;25:1-4.
- Vollaard NB, Shearman JP, Cooper CE. Exercise-induced oxidative stress: myths, realities and physiological relevan- ce. Sports Med 2005;12:1045-62.
- Reilly T, Ekblom B. The Use of recovery methods post-exer- cise. J Sports Sci 2005;6:61927.
- Cazzola R, Russo-Volpe S, Cervato G, Cestaro B. Biochemi- cal assessments of oxidative stress, erythrocyte membrane fluidity and antioxidant status in professional soccer player- sand sedentary controls. Eur J ClinInvest 2003;10:924-30.
- Brites FD, Evelson PA, Christiansen MG, et al. Soccer players under regular training Show oxidative stress but an improved plasma antioxidant status. ClinSci (Lond) 1999;4:381-5.
- Banfi G, Malavazos A, Iorio E, et al. Plasma Oxidative Stress Biomarkers, nitricoxideand heat shock protein 70 in trai- ned elite soccerplayers. Eur J ApplPhysiol 2005;5:483-6.
- Demirbag R, Yilmaz R, Guzel S, et al. Effects of treadmill exercise test on oxidative/antioxidative parameters and DNA damage. Anadolu Kardiyol Derg 2006;6:135-40.
- Michailidis Y, Jamurtas AZ, Nikolaidis MG, et al. Sampling time is crucial for measurement of aerobic exercise-induced oxidative stress. MedSci Sports Exerc 2007;39:1107-13.
- Kurban S, Mehmetoglu I, Yerlikaya HF, Gonen S, Erdem S. Effect of chronic regular exercise on serum ischemia-mo- dified albumin levels and oxidative stress in type 2 diabetes mellitus. Endocr Res 2011;36:116-23.
- Ficicilar H, Zergeroglu AM, Ersoz G, et al. The effects of short-term training on platelet function and total antioxi- dant capacity in rats. Physiol Res 2006;55:151-6.
- Ascensão A, Rebelo A, Oliveira E, et al. Biochemical impa- ct of a soccermatch-analysis of oxidative stress and musc- le damage markers throughout recovery. Clin Biochem 2008;41:841-51.
- Benot S, Goberna R, Reiter RJ, et al. Physiological Levels of melatonin contribute the antioxidant capacity of human serum. J Pineal Res 1999;27:59-64.
- Hammouda O, Chtourou H, Chahed H, et al. Diurnal vari- ations of plasma homocysteine, total antioxidant status, and biological markers of muscle injury during repeated sprint: effect on performance an muscle fatigue--a pilot study. Chronobiol Int 2011;28:958-67.