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Yaşlı Sıçanlarda Antrenman ve Koenzim Q10 Takviyesinin Kalp Dokusunda Oksidatif Stres ve Antioksidan Savunma Belirteçleri Üzerine Etkileri

Yıl 2015, Cilt: 25 Sayı: 2, 52 - 57, 01.06.2015

Muaz Belviranlı Nilsel Okudan Ayşe Özdemir Mehmet Öz

Öz

Amaç: Bu çalışmanın amacı, yaşlı sıçanlarda antrenman ve/veya koenzim Q10 CoQ10 desteğinin kalp dokusunda oksidatif stres ve antioksidan savunma değişiklikleri üzerine etkilerini incelemekti. Gereç ve yöntem: Çalışmada 27 genç 3 aylık ve 27 yaşlı 20 aylık Wistar erkek sıçan kullanıldı. Her bir grup; kontrol, antrenman, CoQ10 ve CoQ10+antrenman olmak üzere dörder alt gruba ayrıldı. Antrenman gruplarındaki sıçanlara 8 hafta boyunca, haftada 5 gün, günde 1 saat yüzme egzersizi yaptırıldı. CoQ10 desteği alan sıçanlara 8 hafta boyunca günde 300 mg/kg CoQ10 oral olarak verildi. Sıçanlar, anestezi altında kalp dokusu örnekleri alındıktan sonra servikal dislokasyonla sakrifiye edildi. Alınan örneklerde malondialdehit MDA , 8-hidroksi–2-deoksiguanozin 8-OHdG , glutatyon GSH seviyeleri ve süperoksit dismutaz SOD ve katalaz CAT aktiviteleri ölçüldü. Bulgular: Gruplar arasında MDA, 8-OHdG ve GSH seviyelerinde herhangi bir fark yoktu. SOD ve CAT aktivitesi genç CoQ10 ve CoQ10+antrenman gruplarında ve tüm yaşlı gruplarda, genç kontrol ve genç antrenman gruplarından düşüktü. Sonuç: Yaşlanma, CoQ10 takviyesi ve antrenman, kalp dokusunda lipid peroksidasyonu, DNA hasarı ve enzimatik olmayan antioksidan seviyeleri üzerine sınırlı etkiye sahipken, antioksidan enzim aktivitesini azaltır

Anahtar Kelimeler

Koenzim Q10 egzersiz yaşlanma kalp oksidatif stres

Kaynakça

  • Soyuer F, Soyuer A. Yaşlılık ve fiziksel aktivite. İnönü Üni Tıp Fak Derg 2008;15:219-24.
  • Jenkins RR. Exercise and oxidative stress methodology: A critique. Am J Clin Nutr 2000;72(2 Suppl):670-4.
  • Halliwell B, Gutteridge JMC. Free radicals in biology and medici- ne. Oxford University Press New York: USA, 2000;534-7.
  • Radak Z, Taylor AW, Ohno H, Goto S. Adaptation to exercise in- duced oxidative stress: from muscle to brain. Exerc Immunol Rev 2001;7:90–107.
  • Greathouse KL, Samuels M, Dimarco NM, Criswell DS. Effe- cts of increased dietary fat and exercise on skeletal muscle lipid peroxidation and antioxidant capacity in male rats. Eur J Nutr 2005;44:429-35.
  • Bloomer RJ, Goldfarb AH. Anaerobic exercise and oxidative stress: A review. Can J Appl Physiol 2004;29:245-63.
  • Gündüz F, Sentürk UK, Kuru O, Aktekin B, Aktekin MR. The effe- ct of one year’s swimming exercise on oxidant stress and antioxi- dant capacity in aged rats. Physiol Res 2004;53:171-6.
  • Mallikarjuna K, Shanmugam KR, Nishanth K, et al. Alcohol-indu- ced deterioration in primary antioxidant and glutathione family enzymes reversed by exercise training in the liver of old rats. Al- cohol 2010;44:523-9.
  • Turunen M, Olsson J, Dallner G. Metabolism and function of co- enzyme Q. Biochim Biophys Acta 2004;1660:171-99.
  • Sohal RS, Forster MJ. Coenzyme Q, oxidative stress and aging. Mi- tochondrion 2007;7:S103-11.
  • Belviranlı M, Okudan N. Well-known antioxidants and newco- mers in sport nutrition: Coenzyme Q10, quercetin, resveratrol, pterostilbene, pycnogenol and astaxanthin. In: Antioxidants in sports nutrition. Ed: Manfred Lamprecht. Florida, USA: CRC Press, Taylor and Francis Group. 2015, 79-102.
  • Kon M, Kimura F, Akimoto T, et al. Effect of Coenzyme Q10 supp- lementation on exercise-induced muscular injury of rats. Exerc Immunol Rev 2007;13:786-8.
  • Faff J, Frankiewicz-Jóźko A. Effect of ubiquinone on exercise-in- duced lipid peroxidation in rat tissues. Eur J Appl Physiol Occup Physiol 1997;75:413-7.
  • Okudan N, Revan S, Balci SS, Belviranli M, Pepe H, Gökbel H. Effects of CoQ10 supplementation and swimming training on ex- haustive exercise-induced oxidative stress in rat heart. Bratisl Lek Listy 2012;113:393-9.
  • Ernst A, Stolzing A, Sandig G, Grune T. Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells. Arch Biochem Biophys 2004;421:54-60.
  • Tomasetti M, Littarru GP, Stocker R, Alleva R. Coenzyme Q10 en- richment decreases oxidative DNA damage in human lymphocy- tes. Free Radic Biol Med 1999;27:1027-32.
  • Kwon DK, Hwang KH, Kim YK, Lee KH, Song YJ. Effects of swimming exercise and soybean supplementation on the immune functions of rats fed a high-fat diet. Clin Exp Pharmacol Physiol 2008;35:638–42.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measure- ment with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
  • Nakao C, Ookawara T, Kizaki T, Oh-Ishi S, Miyazaki H, Haga S, et al. Effects of swimming training on three superoxide dismutase isoenzymes in mouse tissues. J Appl Physiol 2000;88:649-54.
  • Jenkins RR. Exercise, oxidative stress, and antioxidants: A review. Int J Sport Nutr 1993;3:356-75.
  • Parildar H, Dogru-Abbasoglu S, Mehmetçik G, et al. Lipid pe- roxidation potential and antioxidants in the heart tissue of beta-a- lanine- or taurine-treated old rats. J Nutr Sci Vitaminol (Tokyo) 2008;54:61-5.
  • Liu J, Yeo HC, Overvik-Douki E, et al. Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous an- tioxidants. J Appl Physiol 2000;89:21-8.
  • Pozzi R, Rosa JC, Eguchi R, Oller do Nascimento CM, Oyama LM, Aguiar O Jr, et al. Genetic damage in multiple organs of acutely exercised rats. Cell Biochem Funct 2010;28:632-6.
  • Reichhold S, Neubauer O, Bulmer AC, Knasmuller S, Wagner KH. Endurance exercise and DNA stability: is there a link to duration and intensity? Mutat Res 2009;682:28-38.
  • Asami S, Hirano T, Yamaguchi R, Tsurudome Y, Itoh H, Kasai H. Effects of forced and spontaneous exercise on 8-hydroxydeoxy- guanosine levels in rat organs. Biochem Biophys Res Commun 1998;243:678-82.
  • Kakarla P, Vadluri G, Reddy KS, Leeuwenburgh C. Vulnerability of the mid aged rat myocardium to the age-induced oxidative stress: Influence of exercise training on antioxidant defense system. Free Radic Res 2005;39:1211-7.
  • Husain K, Somani SM. Response of cardiac antioxidant system to alcohol and exercise training in the rat. Alcohol 1997;14:301-7.
  • Husain K, Hazelrigg SR. Oxidative injury due to chronic nitric oxi- de synthase inhibition in rat: effect of regular exercise on the heart. Biochim Biophys Acta 2002;1587:75-82.
  • Leeuwenburgh C, Heinecke JW. Oxidative stress and antioxidants in exercise. Curr Med Chem 2001;8:829-38.
  • Terblanche SE. The effects of exhaustive exercise on the activity levels of catalase in various tissues of male and female rats. Cell Biol Int 2000;23:749-53.
  • Nishiyama Y, Ikeda H, Haramaki N, Yoshida N, Imaizumi T. Oxi- dative stress is related to exercise intolerance in patients with heart failure. Am Heart J 1998;135:115-20.
  • Gül M, Demircan B, Taysi S, Oztasan N, Gumustekin K, Siktar E, et al. Effects of endurance training and acute exhaustive exercise on antioxidant defense mechanisms in rat heart. Comp Biochem Physiol A Mol Integr Physiol 2006;143:239-45.
  • Qiao D, Hou L, Liu X. Influence of intermittent anaerobic exercise on mouse physical endurance and antioxidant components. Br J Sports Med 2006;40:214–8.
  • Atalay M, Seene T, Hänninen O, Sen CK. Skeletal muscle and heart antioxidant defences in response to sprint training. Acta Physiol Scand 1996;158:129-34.
  • Tiidus PM, Houston ME. Antioxidant and oxidative enzyme adaptations to vitamin E deprivation and training. Med Sci Sports Exerc 1994;26:354-9.

Yıl 2015, Cilt: 25 Sayı: 2, 52 - 57, 01.06.2015

Muaz Belviranlı Nilsel Okudan Ayşe Özdemir Mehmet Öz

Öz

Objectives: The aim of this study was to investigate the effects of exercise training and/or coenzyme Q10 CoQ10 supplementa- tion on oxidative stress and antioxidant defense changes in heart tissue of aged rats. Material and methods: Twenty-seven young 3-months of age and 27 aged 20-months of age Wistar male rats were used in the study. Each group was further divided into four subgroups: Control, training, CoQ10 and CoQ10+training. The training program consisted of swimming exercise one hour per day, five days a week, for eight weeks. The CoQ10 were given at a daily dose of 300 mg.kg-1 of body weight five days/week via oral gavage. Heart tissues were taken under anesthesia then rats were sacrificed by servical dislocation. Malondialdehyde MDA , 8-hydroxy-2-de- oxyguanosine 8-OHdG , glutathione GSH levels and superoxide dismutase SOD and catalase CAT activities were analyzed in samples.Results: There was no significant difference among the groups with respect to MDA, 8-OHdG and GSH levels. SOD and CAT activities were lower in the young CoQ10 and in the CoQ10 plus training groups than the young control, young training and all aged groups. Conclusion: Although aging, CoQ10 supplementation and exercise training have limited effect on lipid peroxidation, DNA damage and non-enzymatic antioxidant levels, they decreased antioxidant enzyme activity in the heart tissue

Anahtar Kelimeler

Coenzyme Q10 exercise aging heart oxidative stress

Kaynakça

  • Soyuer F, Soyuer A. Yaşlılık ve fiziksel aktivite. İnönü Üni Tıp Fak Derg 2008;15:219-24.
  • Jenkins RR. Exercise and oxidative stress methodology: A critique. Am J Clin Nutr 2000;72(2 Suppl):670-4.
  • Halliwell B, Gutteridge JMC. Free radicals in biology and medici- ne. Oxford University Press New York: USA, 2000;534-7.
  • Radak Z, Taylor AW, Ohno H, Goto S. Adaptation to exercise in- duced oxidative stress: from muscle to brain. Exerc Immunol Rev 2001;7:90–107.
  • Greathouse KL, Samuels M, Dimarco NM, Criswell DS. Effe- cts of increased dietary fat and exercise on skeletal muscle lipid peroxidation and antioxidant capacity in male rats. Eur J Nutr 2005;44:429-35.
  • Bloomer RJ, Goldfarb AH. Anaerobic exercise and oxidative stress: A review. Can J Appl Physiol 2004;29:245-63.
  • Gündüz F, Sentürk UK, Kuru O, Aktekin B, Aktekin MR. The effe- ct of one year’s swimming exercise on oxidant stress and antioxi- dant capacity in aged rats. Physiol Res 2004;53:171-6.
  • Mallikarjuna K, Shanmugam KR, Nishanth K, et al. Alcohol-indu- ced deterioration in primary antioxidant and glutathione family enzymes reversed by exercise training in the liver of old rats. Al- cohol 2010;44:523-9.
  • Turunen M, Olsson J, Dallner G. Metabolism and function of co- enzyme Q. Biochim Biophys Acta 2004;1660:171-99.
  • Sohal RS, Forster MJ. Coenzyme Q, oxidative stress and aging. Mi- tochondrion 2007;7:S103-11.
  • Belviranlı M, Okudan N. Well-known antioxidants and newco- mers in sport nutrition: Coenzyme Q10, quercetin, resveratrol, pterostilbene, pycnogenol and astaxanthin. In: Antioxidants in sports nutrition. Ed: Manfred Lamprecht. Florida, USA: CRC Press, Taylor and Francis Group. 2015, 79-102.
  • Kon M, Kimura F, Akimoto T, et al. Effect of Coenzyme Q10 supp- lementation on exercise-induced muscular injury of rats. Exerc Immunol Rev 2007;13:786-8.
  • Faff J, Frankiewicz-Jóźko A. Effect of ubiquinone on exercise-in- duced lipid peroxidation in rat tissues. Eur J Appl Physiol Occup Physiol 1997;75:413-7.
  • Okudan N, Revan S, Balci SS, Belviranli M, Pepe H, Gökbel H. Effects of CoQ10 supplementation and swimming training on ex- haustive exercise-induced oxidative stress in rat heart. Bratisl Lek Listy 2012;113:393-9.
  • Ernst A, Stolzing A, Sandig G, Grune T. Antioxidants effectively prevent oxidation-induced protein damage in OLN 93 cells. Arch Biochem Biophys 2004;421:54-60.
  • Tomasetti M, Littarru GP, Stocker R, Alleva R. Coenzyme Q10 en- richment decreases oxidative DNA damage in human lymphocy- tes. Free Radic Biol Med 1999;27:1027-32.
  • Kwon DK, Hwang KH, Kim YK, Lee KH, Song YJ. Effects of swimming exercise and soybean supplementation on the immune functions of rats fed a high-fat diet. Clin Exp Pharmacol Physiol 2008;35:638–42.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measure- ment with the Folin phenol reagent. J Biol Chem 1951;193:265-75.
  • Nakao C, Ookawara T, Kizaki T, Oh-Ishi S, Miyazaki H, Haga S, et al. Effects of swimming training on three superoxide dismutase isoenzymes in mouse tissues. J Appl Physiol 2000;88:649-54.
  • Jenkins RR. Exercise, oxidative stress, and antioxidants: A review. Int J Sport Nutr 1993;3:356-75.
  • Parildar H, Dogru-Abbasoglu S, Mehmetçik G, et al. Lipid pe- roxidation potential and antioxidants in the heart tissue of beta-a- lanine- or taurine-treated old rats. J Nutr Sci Vitaminol (Tokyo) 2008;54:61-5.
  • Liu J, Yeo HC, Overvik-Douki E, et al. Chronically and acutely exercised rats: biomarkers of oxidative stress and endogenous an- tioxidants. J Appl Physiol 2000;89:21-8.
  • Pozzi R, Rosa JC, Eguchi R, Oller do Nascimento CM, Oyama LM, Aguiar O Jr, et al. Genetic damage in multiple organs of acutely exercised rats. Cell Biochem Funct 2010;28:632-6.
  • Reichhold S, Neubauer O, Bulmer AC, Knasmuller S, Wagner KH. Endurance exercise and DNA stability: is there a link to duration and intensity? Mutat Res 2009;682:28-38.
  • Asami S, Hirano T, Yamaguchi R, Tsurudome Y, Itoh H, Kasai H. Effects of forced and spontaneous exercise on 8-hydroxydeoxy- guanosine levels in rat organs. Biochem Biophys Res Commun 1998;243:678-82.
  • Kakarla P, Vadluri G, Reddy KS, Leeuwenburgh C. Vulnerability of the mid aged rat myocardium to the age-induced oxidative stress: Influence of exercise training on antioxidant defense system. Free Radic Res 2005;39:1211-7.
  • Husain K, Somani SM. Response of cardiac antioxidant system to alcohol and exercise training in the rat. Alcohol 1997;14:301-7.
  • Husain K, Hazelrigg SR. Oxidative injury due to chronic nitric oxi- de synthase inhibition in rat: effect of regular exercise on the heart. Biochim Biophys Acta 2002;1587:75-82.
  • Leeuwenburgh C, Heinecke JW. Oxidative stress and antioxidants in exercise. Curr Med Chem 2001;8:829-38.
  • Terblanche SE. The effects of exhaustive exercise on the activity levels of catalase in various tissues of male and female rats. Cell Biol Int 2000;23:749-53.
  • Nishiyama Y, Ikeda H, Haramaki N, Yoshida N, Imaizumi T. Oxi- dative stress is related to exercise intolerance in patients with heart failure. Am Heart J 1998;135:115-20.
  • Gül M, Demircan B, Taysi S, Oztasan N, Gumustekin K, Siktar E, et al. Effects of endurance training and acute exhaustive exercise on antioxidant defense mechanisms in rat heart. Comp Biochem Physiol A Mol Integr Physiol 2006;143:239-45.
  • Qiao D, Hou L, Liu X. Influence of intermittent anaerobic exercise on mouse physical endurance and antioxidant components. Br J Sports Med 2006;40:214–8.
  • Atalay M, Seene T, Hänninen O, Sen CK. Skeletal muscle and heart antioxidant defences in response to sprint training. Acta Physiol Scand 1996;158:129-34.
  • Tiidus PM, Houston ME. Antioxidant and oxidative enzyme adaptations to vitamin E deprivation and training. Med Sci Sports Exerc 1994;26:354-9.
Toplam 35 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Original Article
Yazarlar

Muaz Belviranlı Bu kişi benim

Nilsel Okudan Bu kişi benim

Ayşe Özdemir Bu kişi benim

Mehmet Öz Bu kişi benim

Yayımlanma Tarihi 1 Haziran 2015
Yayımlandığı Sayı Yıl 2015 Cilt: 25 Sayı: 2

Kaynak Göster

Vancouver Belviranlı M, Okudan N, Özdemir A, Öz M. Yaşlı Sıçanlarda Antrenman ve Koenzim Q10 Takviyesinin Kalp Dokusunda Oksidatif Stres ve Antioksidan Savunma Belirteçleri Üzerine Etkileri. Genel Tıp Derg. 2015;25(2):52-7.


Genel Tıp Dergisi Creative Commons Atıf-GayriTicari 4.0 Uluslararası Lisansı (CC BY NC) ile lisanslanmıştır.