Increased fat graft survival with mesenchymal stem cell recruiting effect of PRP: in vitro and in vivo study of application techniques

Yıl 2022, Cilt: 32 Sayı: 2, 207 - 214, 29.04.2022

Gokce Yildiran , Osman Akdağ , Gulsemin Cicek Zeliha Esin Celik Hasibe Artaç Hulya Ucaryilmaz Selcuk Duman Mustafa Sutcu , Tahsin Murad Aktan Zekeriya Tosun

https://doi.org/10.54005/geneltip.1074859

Öz

Aim: Adding platelet-rich plasm (PRP) or mesenchymal stem cells are the most accepted methods to increase fat graft’s permanence. However, there is no consensus on timing and whether the effect of stem cells or PRP is observed more in the recipient area or in the donor area.
It is aimed to present the application method and localization of PRP to be combined with fat graft to increase the survival.
Material and Methods: in vitro part: Fat grafts were kept in a medium containing PRP. Cell output from the fat graft to the Petri dishes was examined every day. The time to recruit the maximum number of stem cells to the medium was determined (day 10). in vivo part: Eight group of rats were received PRP in different concentrations either to recipient or donor areas of fat grafts. After ten days, fat grafts were transferred to the recipient area. Ninety days after the transfer, histopathological examination and immunohistochemical staining were performed.
Results: The group which had received the full-dose PRP to the recipient area showed the highest cellular integrity and vascularity (p< 0.05). Vascularity was superior in the half-dose PRP group compared to the control group (p< 0.05); cellular integrity did not increase. Donor site groups did not show increase in cellular integrity.
Conclusion: Although PRP starts to increase fat graft permanence by increasing vascularity, it acts by preserving the cellular integrity of the fat cells as the concentrations increase. The PRP injection to the recipient area 10 days before fat grafting may provide higher survival rates.

Anahtar Kelimeler

fat graft, PRP, stem cell, survival

Destekleyen Kurum

Selçuk Üniversitesi Bilimsel Araştırma Projeleri

Proje Numarası

17102016 ve 19401147

Teşekkür

This study was produced from the first author's thesis. Thank you to the staff at the SUDAM animal laboratory and SU scientific research project unit for the support.

PRP’nin mezenkimal kök hücre çağırıcı etkisi ile yağ grefti sağkalımında artış: Uygulama tekniklerinin in vitro ve in vivo çalışması

Öz

Amaç: Yağ greftinin kalıcılığını artırmak için trombositten zengin plazma (PRP) veya mezenkimal kök hücre eklenmesi en çok kabul gören yöntemlerdir. Ancak zamanlama ve bölge açısından kök hücrenin mi yoksa PRPnin mi etkisinin daha çok gözlendiği konusunda bir fikir birliği yoktur.
Sağkalımı artırmak için yağ grefti ile kombine edilecek PRP'nin uygulama yöntemi ve lokalizasyonunun sunulması amaçlanmaktadır.
Gereç ve Yöntem: in vitro çalışma: Yağ greftleri PRP içeren besiyerinde tutulmuştur. Yağ greftinden Petri kaplarına hücre çıkışı her gün incelendi. Ortama en fazla sayıda kök hücre alma zamanı belirlendi (10. gün).
in vivo çalışma: Sekiz grup sıçana, yağ greftlerinin alıcı veya verici alanlarına farklı konsantrasyonlarda PRP uygulandı. On gün sonra alıcı bölgeye yağ greftleri transfer edildi. Transferden doksan gün sonra histopatolojik inceleme ve immünohistokimyasal boyama yapıldı.
Bulgular: Alıcı alana tam doz PRP uygulanan grup en yüksek hücresel bütünlük ve damarlanma gösterdi (p< 0.05). Yarım doz PRP grubunda vaskülarite kontrol grubuna göre daha üstündü (p< 0.05); hücresel bütünlük artmadı. Alıcı alan grupları, hücresel bütünlükte artış göstermedi.
Sonuç: PRP damarlanmayı artırarak yağ grefti kalıcılığını artırmaya başlasa da konsantras-yonlar arttıkça yağ hücrelerinin hücresel bütünlüğünü koruyarak etki eder. Yağ greftlemesinden 10 gün önce alıcı bölgeye PRP enjeksiyonu daha yüksek hayatta kalma oranları sağlayabilir.

Anahtar Kelimeler

kök hücre, PRP, sağkalım, yağ grefti

Proje Numarası

17102016 ve 19401147

Kaynakça

• 1. Simonacci F, Bertozzi N, Grieco MP, Grignaffini E, Raposio E. Procedure, applications, and outcomes of autologous fat grafting. Ann Med Surg (Lond) 2017;20:49-60.
• 2. Li F, Guo W, Li K, et. al. Improved fat graft survival by different volume fractions of platelet-rich plasma and adipose-derived stem cells. Aesthet Surg J 2015;35:319-33.
• 3. Xiong BJ, Tan QW, Chen YJ, et. al. The Effects of Platelet-Rich Plasma and Adipose-Derived Stem Cells on Neovascularization and Fat Graft Survival. Aesthetic Plast Surg 2018;42:1-8.
• 4. Hu Y, Jiang Y, Wang M, Tian W, Wang H. Concentrated Growth Factor Enhanced Fat Graft Survival: A Comparative Study. Dermatol Surg 2018;44:976-84.
• 5. Cervelli V, De Angelis B, Lucarini L, et. al. Tissue regeneration in loss of substance on the lower limbs through use of platelet-rich plasma, stem cells from adipose tissue, and hyaluronic acid. Adv Skin Wound Care 2010;23:262-72.
• 6. Houdek MT, Wyles CC, Stalboerger PG, et. al. Collagen and Fractionated Platelet-Rich Plasma Scaffold for Dermal Regeneration. Plast Reconstr Surg 2016;137:1498-506.
• 7. Sezgin B, Ozmen S, Bulam H, et. al. Improving fat graft survival through preconditioning of the recipient site with microneedling. J Plast Reconstr Aesthet Surg 2014;67:712-20.
• 8. Rohrich RJ, Sorokin ES, Brown SA. In search of improved fat transfer viability: a quantitative analysis of the role of centrifugation and harvest site. Plast Reconstr Surg 2004;113:391‐7.
• 9. Kassolis JD, Reynolds MA. Evaluation of the adjunctive benefits of platelet-rich plasma in subantral sinus augmentation. J Craniofac Surg 2005;16:280-7.
• 10. Rasmussen BS, Sørensen CL, Kurbegovic S, et. al. Cell-Enriched Fat Grafting Improves Graft Retention in a Porcine Model: A Dose-Response Study of Adipose-Derived Stem Cells versus Stromal Vascular Fraction. Plast Reconstr Surg 2019;144:397e‐408e.
• 11. Zhao J, Yi C, Zheng Y, et. al. Enhancement of fat graft survival by bone marrow-derived mesenchymal stem cell therapy. Plast Reconstr Surg 2013;132:1149-57.
• 12. Atashi F, André-Lévigne D, Colin DJ, et. al. Does non-activated platelet-rich plasma (PRP) enhance fat graft outcome? An assessment with 3D CT-scan in mice. J Plast Reconstr Aesthet Surg 2019;72:669‐75.
• 13. Raposio E, Bertozzi N, Bonomini S, et. al. Adipose-derived Stem Cells Added to Platelet-rich Plasma for Chronic Skin Ulcer Therapy. Wounds 2016;28:126-31.
• 14. Ferraro GA, De Francesco F, Tirino V, et. al. Effects of a new centrifugation method on adipose cell viability for autologous fat grafting. Aesthetic Plast Surg 2011;35:341-8.
• 15. Son D, Oh J, Choi T, et. al. Viability of fat cells over time after syringe suction lipectomy: the effects of cryopreservation. Ann Plast Surg 2010;65:354-60.