MOLECULAR EFFECTS OF QUINOA ON RAT KIDNEY: A DOSE-DEPENDENT STUDY ON PYROPTOSIS AND ANTIOXIDANT DEFENSE

Öz

Oxidative stress and inflammation contribute to renal dysfunction, and antioxidant-rich functional foods may modulate these processes. This study evaluated the effects of dietary quinoa on oxidative stress and the expression of pyroptosis, inflammation and apoptosis-related genes in healthy rat kidneys. Forty male Wistar albino rats were randomly assigned to control and diet groups containing 5%, 10% or 20% quinoa for six weeks (n=10). Rat body weight, superoxide dismutase activity, glutathione, and malondialdehyde levels in kidney tissue were measured; expression of nine genes was assessed in a representative subgroup using quantitative real-time polymerase chain reaction. Quinoa dose-dependently increased antioxidant markers, reduced lipid peroxidation, and downregulated inflammasome (Nlrp3, Casp-1,Gsdmd), inflammatory (Nf-κB, IL-1β, Tnf-α), and apoptotic (Bax,Casp-3) genes, while increasing Bcl2 expression, with the greatest effects at 10% and 20% quinoa. These findings provide evidence that dietary quinoa beneficially modulates renal molecular pathways involved in oxidative stress and inflammatory responses under physiological conditions.

Anahtar Kelimeler

• Antioxidants
• quinoa
• inflammasomes
• kidney
• oxidative stress
• pyroptosis

Etik Beyan

The study protocol was reviewed and approved by the Local Ethics Committee for Animal Experiments of Atatürk University (HADYEK; meeting date 30.03.2026, session no. 2026/04, decision no. 44).

KİNOANIN SIÇAN BÖBREĞİ ÜZERİNDEKİ MOLEKÜLER ETKİLERİ: PİROPTOZ VE ANTİOKSİDAN SAVUNMA ÜZERİNE DOZA BAĞLI BIR ÇALIŞMA

Öz

Oksidatif stres ve inflamasyon böbrek fonksiyon bozukluğuna katkıda bulunur ve antioksidan açısından zengin fonksiyonel gıdalar bu süreçleri düzenleyebilir. Bu çalışma, diyetle alınan kinoanın sağlıklı sıçan böbreklerinde oksidatif stres ve piroptoz, inflamasyon ve apoptoz ile ilgili genlerin ekspresyonu üzerindeki etkilerini değerlendirdi. Kırk erkek Wistar albino sıçan, altı hafta boyunca rastgele kontrol ve %5, %10 veya %20 kinoa içeren diyet gruplarına ayrıldı (n=10). Sıçan vücut ağırlığı, süperoksit dismutaz aktivitesi, glutatyon ve böbrek dokusundaki malondialdehit seviyeleri ölçüldü; dokuz genin ekspresyonu, kantitatif gerçek zamanlı polimeraz zincir reaksiyonu kullanılarak temsili bir alt grupta değerlendirildi. Kinoa, doz bağımlı olarak antioksidan belirteçlerini artırdı, lipid peroksidasyonunu azalttı ve inflamasyon (Nlrp3, Casp-1, Gsdmd), inflamasyon (Nf-κB, IL-1β, Tnf-α) ve apoptotik (Bax, Casp-3) genlerini aşağı yönlü düzenlerken, Bcl2 ekspresyonunu artırdı; en büyük etkiler %10 ve %20 kinoa oranlarında gözlendi. Bu bulgular, diyetle alınan kinoanın fizyolojik koşullar altında oksidatif stres ve inflamatuar yanıtlarla ilgili böbrek moleküler yollarını faydalı bir şekilde düzenlediğine dair kanıt sağlamaktadır.

Anahtar Kelimeler

• Antioksidanlar
• kinoa
• inflamazomlar
• böbrek
• oksidatif stress
• piroptozis

Etik Beyan

Çalışma protokolü, Atatürk Üniversitesi Yerel Hayvan Deneyleri Etik Kurulu (HADYEK) tarafından incelenmiş ve onaylanmıştır (toplantı tarihi 30.03.2026, oturum no. 2026/04, karar no. 44).

Kaynakça

1. Abd-Ellatif, R. N., Nasef, N. A., El-Horany, H. E., Emam, M. N., Younis, R. L., El Gheit, R. E. A., Elseady, W., Radwan, D. A., Hafez, Y. M., Eissa, A., Aboalsoud, A., Shalaby, R. H., & Atef, M. M. (2022). Adrenomedullin Mitigates Doxorubicin-Induced Nephrotoxicity in Rats: Role of Oxidative Stress, Inflammation, Apoptosis, and Pyroptosis. Int J Mol Sci, 23(23). https://doi.org/10.3390/ijms232314570
2. Abdel-Wahhab, K. G., Mannaa, F. A., Ashry, M., Khaled, D. M., Hassan, L. K., & Gomaa, H. F. (2021). Chenopodium quinoa ethanolic extract ameliorates cyclophosphamide®-induced hepatotoxicity in male rats. Comparative Clinical Pathology, 30(2), 267-276.
3. Adiga, M., Manjula, S., Punja, D., Sadacharan, C. M., Upadhya, D., Vasanthalaxmi, K., Acharya, N., & Suryavanshi, C. (2025). Short-term consequences of daily consumption of the quinoa (Chenopodium quinoa Willd.) diet in normal and diabetic rats. Veterinary World, 18(6), 1715.
4. Agarwal, A., Rizwana, Tripathi, A. D., Kumar, T., Sharma, K. P., & Patel, S. K. S. (2023). Nutritional and functional new perspectives and potential health benefits of quinoa and chia seeds. Antioxidants, 12(7), 1413.
5. Akaras, N., Gur, C., Kucukler, S., & Kandemir, F. M. (2023). Zingerone reduces sodium arsenite-induced nephrotoxicity by regulating oxidative stress, inflammation, apoptosis and histopathological changes. Chem Biol Interact, 374, 110410. https://doi.org/10.1016/j.cbi.2023.110410
6. Al-Qabba, M. M., El-Mowafy, M. A., Althwab, S. A., Alfheeaid, H. A., Aljutaily, T., & Barakat, H. (2020). Phenolic profile, antioxidant activity, and ameliorating efficacy of chenopodium quinoa sprouts against CCl4-induced oxidative stress in rats. Nutrients, 12(10), 2904.
7. Bozkurt, İ., & Halıcı, MB. (2026). Effects of Borage Oil on Oxidative Damage, Inflammatory Response (IL-6, IL-1β, and TNF- α), and Signaling Pathways (Bax/Bcl-2 and Caspase-3) in Lead Acetate–Induced Nephrotoxicity. Pharmata, 6(1), 10-17. https://doi.org/https://doi.org/10.62425/pharmata.1868902
8. Bozkurt, I., Halici, Z., Zirh, E. B., & Palabiyik-Yucelik, S. S. (2024). EGCG alleviates Ochratoxin A-induced pyroptosis in rat's kidney by inhibiting NLRP3/Caspase-1/GSDMD signaling pathway. Food and Chemical Toxicology, 193, 115006.

9. Cisneros‐Yupanqui, M., Lante, A., Mihaylova, D., Krastanov, A. I., & Vílchez‐Perales, C. (2020). Impact of consumption of cooked red and black Chenopodium quinoa Willd. over blood lipids, oxidative stress, and blood glucose levels in hypertension‐induced rats. Cereal Chemistry, 97(6), 1254-1262.
10. Dhouibi, R., Affes, H., Ben Salem, M., Charfi, S., Marekchi, R., Hammami, S., Zeghal, K., & Ksouda, K. (2021). Protective effect of Urtica dioica in induced neurobehavioral changes, nephrotoxicity and hepatotoxicity after chronic exposure to potassium bromate in rats. Environ Pollut, 287, 117657. https://doi.org/10.1016/j.envpol.2021.117657
11. Dong, W., Zhang, K., Wang, X., Li, J., Zou, H., Yuan, Y., Gu, J., Zhu, J., Liu, G., Liu, Z., & Song, R. (2024). SIRT1 alleviates Cd nephrotoxicity through NF-κB/p65 deacetylation-mediated pyroptosis in rat renal tubular epithelial cells. Sci Total Environ, 929, 172392. https://doi.org/10.1016/j.scitotenv.2024.172392
12. El-Gohary, R. M., Ghalwash, A. A., Awad, M. M., El-Shaer, R. A. A., Ibrahim, S., Eltantawy, A. F., Elmansy, A., & Okasha, A. H. (2023). Novel insights into the augmented effect of curcumin and liraglutide in ameliorating cisplatin-induced nephrotoxicity in rats: Effects on oxidative stress, inflammation, apoptosis and pyroptosis via GSK-3β. Arch Biochem Biophys, 749, 109801. https://doi.org/10.1016/j.abb.2023.109801
13. Fu, Y., Cao, J., Wei, X., Ge, Y., Su, Z., & Yu, D. (2023). Klotho alleviates contrast-induced acute kidney injury by suppressing oxidative stress, inflammation, and NF-KappaB/NLRP3-mediated pyroptosis. Int Immunopharmacol, 118, 110105. https://doi.org/10.1016/j.intimp.2023.110105
14. Graf, B. L., Rojas‐Silva, P., Rojo, L. E., Delatorre‐Herrera, J., Baldeón, M. E., & Raskin, I. (2015). Innovations in health value and functional food development of quinoa (Chenopodium quinoa Willd.). Comprehensive reviews in food science and food safety, 14(4), 431-445.
15. Gur, C., Kandemir, F. M., Caglayan, C., & Satıcı, E. (2022). Chemopreventive effects of hesperidin against paclitaxel-induced hepatotoxicity and nephrotoxicity via amendment of Nrf2/HO-1 and caspase-3/Bax/Bcl-2 signaling pathways. Chem Biol Interact, 365, 110073. https://doi.org/10.1016/j.cbi.2022.110073
16. Ibrahim, R. M., Abdel Moneim, A. E., Ramadan, S. S., & Abdalla, M. S. (2025). The Biological Effect of Chenopodium quinoa willd.(quinoa) Seeds with Insight into Its Physiological Impact on The Liver. Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology, 17(1), 81-98.
17. Li, H., Mao, X., Liu, K., Sun, J., Li, B., Malyar, R. M., Liu, D., Pan, C., Gan, F., & Liu, Y. (2021). Ochratoxin A induces nephrotoxicity in vitro and in vivo via pyroptosis. Archives of Toxicology, 95(4), 1489-1502.
18. Lin, X., Zhou, Q., Zhou, L., Sun, Y., Han, X., Cheng, X., Wu, M., Lv, W., Wang, J., & Zhao, W. (2023). Quinoa (Chenopodium quinoa Willd) bran saponins alleviate hyperuricemia and inhibit renal injury by regulating the PI3K/AKT/NFκB signaling pathway and uric acid transport. Journal of Agricultural and Food Chemistry, 71(17), 6635-6649.
19. Little, A., Murphy, K., & Solverson, P. (2021). Quinoa’s potential to enhance dietary Management of Obesity and Type-2 diabetes: A review of the current evidence. Diabetology, 2(2), 77-94.
20. Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods, 25(4), 402-408. https://doi.org/10.1006/meth.2001.1262 Melios, S., Clegg, M., & Grasso, S. (2025). Exploring the Health Benefits of Quinoa (Chenopodium Quinoa Willd.): A Review of the Recent Literature. Food Reviews International, 41(3), 953-977.
21. Omran, N. H., El-Bahy, A. A., Hosny, H. T. A., & Handoussa, H. (2023). Quinoa and Chia modulate AMPK/PPAR-ɣ signaling in high-fat diet–induced obesity rat model. Revista Brasileira de Farmacognosia, 33(3), 583-594.
22. Placer, Z. A., Cushman, L. L., & Johnson, B. C. (1966). Estimation of product of lipid peroxidation (malonyl dialdehyde) in biochemical systems. Anal Biochem, 16(2), 359-364. https://doi.org/10.1016/0003-2697(66)90167-9
23. Sedlak, J., & Lindsay, R. H. (1968). Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem, 25(1), 192-205. https://doi.org/10.1016/0003-2697(68)90092-4 Sun, Y., Oberley, L. W., & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical Chemistry, 34(3), 497-500. https://doi.org/10.1093/clinchem/34.3.497
24. Tang, Y., Li, X., Chen, P. X., Zhang, B., Hernandez, M., Zhang, H., Marcone, M. F., Liu, R., & Tsao, R. (2015). Characterisation of fatty acid, carotenoid, tocopherol/tocotrienol compositions and antioxidant activities in seeds of three Chenopodium quinoa Willd. genotypes. Food chemistry, 174, 502-508.
25. Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M., & Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. The international journal of biochemistry & cell biology, 39(1), 44-84.
26. Yosri, H., El-Kashef, D. H., El-Sherbiny, M., Said, E., & Salem, H. A. (2022). Calycosin modulates NLRP3 and TXNIP-mediated pyroptotic signaling and attenuates diabetic nephropathy progression in diabetic rats; An insight. Biomed Pharmacother, 155, 113758. https://doi.org/10.1016/j.biopha.2022.113758