LACTOBACILLUS DELBRUECKII SUBSP. BULGARICUS ZN541 VE STREPTOCOCCUS THERMOPHILUS Z1052 SUŞLARINDA BETA GALAKTOZİDAZ AKTİVİTESİ VE OPTİMİZASYON

Öz

Çalışmada, 31 Lactobacillus delbrueckii subsp. bulgaricus ve 34 Streptococcus thermophilus bakteri kültürlerinde, o-nitrofenil-beta-D-galaktosit (o-NPG) substrat olarak kullanılarak, β-galaktosidaz enzim ve spesifik aktiviteleri taranmıştır. L. delbrueckii subsp. bulgaricus suşları 0.186-6.500 U/mg arasında spesifik aktivite gösterirken, S. thermophilus suşları 0.172-5.064 U/mg arasında spesifik aktivite göstermiştir. L. delbrueckii subsp. bulgaricus ZN541 (6.500 U/mg) ve S. thermophilus Z1052 (5.064 U/mg) suşlarının yüksek spesifik aktivite yeteneğine sahip oldukları tespit edilmiştir. Yüksek spesifik β-galaktozidaz aktivitesi gösteren ZN541 ve Z1052 suşları seçilerek, farklı koşulların (pH, sıcaklık, laktoz konsantrasyonu ve fermantasyon süresi) bu suşlara ait β-galaktozidaz enzimlerin aktivitelerine etkileri belirlenmiştir. ZN541 suşunda optimum enzim aktivitesi için gereken pH’ın 6.2, sıcaklığın 42°C, laktoz konsantrasyonunun %2 ve fermantasyon süresinin 24 saat olduğu tespit edilmiştir. Z1052 suşunda ise optimum enzim aktivitesi için gereken pH’ın 6.8, sıcaklığın 42°C, laktoz konsantrasyonun %4 ve fermantasyon süresinin 24 saat olduğu belirlenmiştir.

Anahtar Kelimeler

• L. delbrueckii ssp. bulgaricus
• S. thermophilus
• β-galaktosidaz
• Optimizasyon

BETA GALACTOSIDASE ACTIVITY IN LACTOBACILLUS DELBRUECKII SUBSP. BULGARICUS ZN541 AND STREPTOCOCCUS THERMOPHILUS Z1052 STRAINS AND OPTIMIZATION

Öz

In this study, thirty-one Lactobacillus delbrueckii subsp. bulgaricus and thirty-four Streptococcus thermophilus were screened for β-galactosidase enzyme activities and specific activities. The β-galactosidase enzyme activities were determined by using o-nitrophenyl-beta-D-galactopyranoside (o-NPG) as a substrate. L. delbrueckii bulgaricus strains showed specific activity between 0.186-6.500 U/mg, while S. thermophilus strains exhibited specific activity between 0.172-5.064 U/mg. The highest specific enzyme activities among bacteria cultures were determined at L. delbrueckii subsp. bulgaricus ZN541 (6.500 U/mg) and S. thermophilus Z1052 (5.064 U/mg) strains. The ZN541 and Z1052 strains that showed high specific β-galactosidase activity were selected and the effects of different conditions (pH, temperature, lactose concentration, and fermentation time) on the activities of β-galactosidase enzymes of these strains were determined. It was determined that the pH required for optimum enzyme activity in ZN541 strain was 6.2, the temperature was 42°C, the lactose concentration was 2% and the fermentation time was 24 hours. In the Z1052 strain, the pH required for optimum enzyme activity was 6.8, the temperature was 42°C, the lactose concentration was 4%, and the fermentation time was 24 hours.

Anahtar Kelimeler

• L. delbrueckii subsp. bulgaricus
• Streptococcus thermophiles
• β-galactosidase
• Optimization

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