EKZOPOLİSAKKARİT ÜRETEN VE ÜRETMEYEN KÜLTÜR KULLANIMININ TULUM PEYNİRLERİNİN SERBEST YAĞ ASİDİ KOMPOZİSYONU ÜZERİNE ETKİLERİ

Yıl 2015, Cilt: 40 Sayı: 4, 201 - 208, 01.08.2015

C Aylin Oluk Mehmet Güven

Öz

Bu çalışmada, ekzopolisakkarit üreten (EPS +) ile ekzopolisakkarit üretmeyen (EPS -) yarım yağlı veEPS (-) tam yağlı Tulum peynirleri 90 gün süreyle olgunlaştırılmış ve depolama süresince serbest yağasidi kompozisyonu incelenmiştir. İncelenen 10 adet serbest yağ asidi arasında palmitik, oleik ve miristikasitler baskın serbest yağ asitleri olarak belirlenmiştir. Tam yağlı ve EPS (-) kültür ile üretilen tulumpeynirinin en yüksek serbest yağ asidi içeriğine sahip olduğu belirlenirken, yarım yağlı EPS (+) vestarter kültür kombinasyonunda L. helveticus olan tulum peyniri en düşük serbest yağ asidi içeriği değerigöstermiştir. EPS (+) kültür kullanımı peynirlerin serbest yağ asidi kompozisyonunu etkilememiştir.Depolama süresine bağlı olarak peynirlerin serbest yağ asidi miktarı artmıştır

Anahtar Kelimeler

Tulum Peyniri, ekzopolisakkarit, serbest yağ asidi, gaz kromatografisi, depolama

EFFECTS OF USE OF EXOPOLYSACCARIDE PRODUCING AND NON PRODUCING CULTURE ON FREE FATTY ACID COMPOSITION OF TULUM CHEESE

Öz

In this study, low fat Tulum cheeses made by EPS (+) and non EPS (-) and full-fat Tulum cheesesproduced by EPS (-) cultures were ripened for 90 days and free fatty acid composition was analysedduring ripening. Palmitic, oleic and myristic acids were the predominant free fatty acids in the 10 freefatty acid investigaded. While full-fat Tulum cheese produced by non-EPS culture had the highest freefatty acid level, low fat Tulum cheeses produced by EPS (+) which contained L. helveticus in starterculture combination showed lowest free fatty acid value. Use of exopolysaccaride producing culturesdid not influence free fatty acid composition. The amount of free fatty acid increased during storage

Anahtar Kelimeler

Tulum cheese, exopolysaccaride, free fatty acid, gas chromotography, storage

Kaynakça

• Hayaloğlu AA. 2003. Starter olarak kullanılan bazı Lactococcus suşlarının beyaz peynirlerin özellikleri ve olgunlaşmaları üzerine etkileri. Çukurova Üniversitesi Fen Bilimleri Enstitüsü Doktora Tezi, Adana, Türkiye, 170s.
• Yaygın H ve Kılıç S. 1993. Süt Endüstrisinde Saf Kültür. Altındağ Matbaacılık, İzmir, Türkiye, 108 s.
• Kavas G, Oysun G, Kınık O, Uysal H. 2004. Effect of some fat replacers on chemical, physical and sensory attributes of low-fat white pickled cheese. Food Chem., 88: 381-388.
• Bryant A, Ustunol, Z. 1995. Consumer acceptance of Cheddar Cheese as influenced by fat reduction. J. Food Tech. 30-4: 26-28.
• Shah NP. 2003. The exopolysaccharide production by starter cultures and their influence on texturel characteristics of fermented milk. Int Dairy Fed, 101-105.
• Welman AD, Maddox, IS. 2003. Exopolysaccharides from lactic acid bacteria: perspectives and challenges. Trends in Biotech. 21: 269-274.
• Cummings JH, Englyst HN. 1995. Gastrointestinal effects of foodcarbohyd rate. American J Clin Nutrition, 61: 938-945.
• Harris PJ, Ferguson LR. 1993. Dietary fibre: Its composition and role in protection against colorectal cancer. Mutation Res. 290: 97-110.
• Robinson RK. 1985. Dairy Microbiol. Volume 1 The Microbiology of Milk, 51-59.
• Low D, Ahlgren JA, Horne D, McMahon DJ, Oberg CJ, Broadbent JR. 1998. Role of Streptococcus thermophilus MR- 1C capsular exopolysaccharide in cheese moisture retention. Appl. Environ. Microbiol, 64: 2147- 2151.
• Champagne CP, Barrette J, Roy D, Rodrigue N. 2006. Fresh-cheesemilk formulation fermented by a combination of freeze-dried citrate-positive cultures and exopolysaccharide-producing lactobacilli with liquid lactococcal starters. Food Res. Int. 39: 651-659
• Hayaloglu AA, Fox, PF, Guven M, Cakmakcı S. 2007. Cheeses of Turkey: 1. Varieties ripened in goat-skin bags. Lait, 87: 79-95.
• Guler Z, Uraz, T. 2003. Proteolytic and lipolytic composition of Tulum Cheeses. Milchwissenschaft, 9 (10): 502-505.
• Larrayoz P, Martinez MT, Barron LJ, Torre P, Barcine Y. 1999. The evolution of free fatty acids during the ripening of Idiazabal cheese: Influence of rennet type. European Food Res Tech, 210: 9-12. 15. Anonymous, 2006. TS 3001 Tulum peyniri Standardı, Türk Standartları Enstitüsü, Ankara, Türkiye, 9 s.
• Nunez M, Garsia-Aser C, Rodriguaz-Martin, MA, Medina M, Gaya P. 1986. The effect of ripening and cooking temperatures on proteolysis and lipolysis in Manchego cheese. Food Chem., 21: 115-123.
• Rolf, FJ. 1993. NTSYS-pc, Numerical Taxonomy and Multivariate Analysis System,version 1.18 New York, Exeter, Setauket.
• Kılıç S. 2001. Süt Endüstrisinde Laktik Asit Bakterileri, Ege Üniv. Ziraat Fak. Yayınları, İzmir, Türkiye, 542s.
• Ordonez AI, Ibanez FC, Torre P, Barcina Y. 1999. Effect of ewes-milk pasteurization on the free amino acids in Idiazabal cheese. Int Dairy J, 9: 135-141.
• Kondyli E, Massouras T, Katsiari MC, Voutsinas LP. 2003a. Free fatty acids and volatile compounds in low-fat Kefalograviera-type cheese made with commercial adjuct cultures. Int Dairy J, 13: 47-54. 21. McSweeney PLH. 2004. Biochemistry of cheese ripening. Int J Dairy Tech, 57 (2/3): 127-143.
• Hayaloğlu AA, Fox PF, Güven M, Çakmakcı S. 2007b. Cheeses of Turkey: 1. Varieties ripened in goat-skin bags. Lait, 87: 79-95.
• Yılmaz G, Ayar A, Akın N. 2005. The effect of microbial lipase on the lipolysis during the ripening of Tulum cheese. J Food Eng, 69: 269-274.
• Buffa M, Guamis B, Pavia M, Trujillo AJ. 2001. Lipolysis in cheese made from raw, pasteurized or high-pressure-treated goats'milk. Int Dairy J, 11: 175-179.
• Kondyli E, Massouras T, Katsiari MC, Voutsinas LP. 2003b. Lipolysis and volatile compounds in low-fat Kefalograviera-type cheese made with commercial special starter cultures. Food Chem., 82: 203-209.
• Kınık O, Gürsoy O, Seçkin AK. 2005. Cholesterol content and fatty acid composition of most consumed Turkish hard and soft cheeses. Czech J Food Sci, 4: 166-172.
• Voight DD, Chevalier F, Donaghy JA, Patterson MF, Qian MC, Kelly AL. 2012. Effect of high-pressure treatment of milk for cheese manufacture on proteolysis, lipolysis, texture and functionality of Cheddar cheese during ripening. Innovative Food Sci Emerging Tech, 13: 23-30.
• Mallatou H, Pappa EC, Massouras T. 2003. Changes in free fatty acids during ripening of Teleme cheese made with ewes’, goats’, cows'or a mixture of ewes'and goats'milk. Int Dairy J, 13: 211-219.
• Kondyli E, Katsiari MC, Massouras T, Voutsinas LP. 2002. free fatty acids and volatile compound of low fat feta-type cheese made with a commer- cial adjunct culture. Food Chem., 79 (2): 199-205.