BibTex RIS Kaynak Göster

Beta Galactosidase Enzyme Activities of Lactobacillus and Bifidobacterium Genus (Turkish with English Abstract)

Yıl 2014, Cilt: 39 Sayı: 4, 211 - 218, 01.08.2014

Öz

In this study total 42 bacteria were used; 39 Lactobacilllus originated from human, food and animal and 3 Bifidobacterium isolated from new born baby feces. The β-galactosidase enzyme activities and specific activities were determined by using o-nitrophenyl-b-D-galactopyranoside (o-NPG) as a substrate. The highest specific enzyme activities observed among Lactobacilli cultures were at L. fermentum ZYN17 (2.468 U/mg), L. casei LB65 (1.116 U/mg), L. rhamnosus GD11 (1.034 U/mg), and L. acidophilus BAZ36 (0.947 U/mg) strains and the highest enzyme activities observed among Bifidobacterium was at B. breve A26 (0.726 U/mg) strain. The enzyme activities of bacteria were also determined qualitatively by using the 5-bromo-4-chloro-3-indolyl-β-D-galaktoside (X-gal) substrate compound. The enzyme optimization of ZYN17 strain with highest specific β-galactosidase activity was achieved. The optimum pH was determined as 6.8, optimum temperature was 37 ºC and the optimum buffer was potassium phosphate buffer for the enzyme.

Kaynakça

  • Gheytanchi E, Heshmati F, Shargh KB, Nowroozi J, Movahedzadeh F. 2010. Study on E-galactosidase enzyme produced by isolated lactobacilli from milk and cheese. Afr J Microbiol Res, 4, 454-458.
  • Karasova P, Spiwok V, Mala S, Kralova B, Russell NJ. 2002. Beta-galactosidase activity in psychrophic microorganisms and their potential use in food industry. Czech J Food Sci, 20(2), 43-47.
  • Verma LM, Barrow JC, Kennedy JF, Puri M. 2012. Immobilization of E-d-galactosidase from Kluyveromyces lactison functionalized silicon dioxide nanoparticles: Characterization and lactose hydrolysis. Int J Biol Macromol, 50(2), 432-437.
  • Panesar R, Panesar PS, Singh RS, Kennedy JF. 2011. Hydrolysis of milk lactose in a packed bed reactor system using immobilized yeast cells. J Chem Technol Biotechnol, 86(1), 42-46.
  • Vonk RJ, Reckman GAR, Harmsen HJM, Priebe MG. 2012. Probiotics and Lactose Intolerance. Chapter 7. InTech Open, 7, 149-160.
  • Singh VP, Sharma J, Babu S, Rizwanulla, Singla A. 2013. Role of probiotics in health and disease: a review. J Pak Med Assoc, 63(2), 253-257.
  • Setty-Shah N, Maranda L, Candela N, Fong J, Dahod I, Rogol AD, Nwosu BU. 2013. Lactose Intolerance: Lack of Evidence for Short Stature or Vitamin D Deficiency in Prepubertal Children. PLOS ONE, 8, 1-9.
  • Bayhan A, Yentür G. 1993. Laktoz ‹ntoleransı. GIDA, 18 (6), 385-388.
  • Rolfe RD. 2000. The role of probiotic cultures in the control of gastrointestinal health. J Nutr, (Supplement).130 (2), 396-402.
  • Sanders ME. 1999. Probiotics. Food Technol, 53 (11), 67-77.
  • De Vrese M, Stegelmann A, Richter B, Fenselau S, Laue C, Schrezenmeir J. 2001. Probiotics- compensation for lactase insufficiency. Am J Clin Nutr, 73 (2), 421-429.
  • Zhang W, Wang C, Huang CY, Yu Q, Liu HC, Zhang CW, Pei XF, Xu X, Wang GQ. 2012. Analysis of E-galactosidase production and their genes of two strains of Lactobacillus bulgaricus. Biotechnol Lett, 34 (6), 1067-1071.
  • Shah NP, Otieno DO. 2007. Endogenous E-glucosidase and E-galactosidase activities from selected probiotic microorganisms and their role in isoflavone biotransformation in soymilk. J Appl Microbiol, 103 (4), 910-917.
  • Kara F. 2004. Release and characterization of beta-galactosidase from Lactobacillus plantarum. M.C. Thesis, Department of Biotechnology, Middle East Technical University, 89p.
  • Ismail SAA, El-Mohamady Y, Helmy WA, Abou-Romia R, Hashem AM. 2010. Cultural condition affecting the growth and production of E-galactosidase by Lactobacillus acidophilus NRRL 4495. Aust J Basic Appl Sci, 4 (10), 5051-5058.
  • Citti JE, Sandine WE, Elliker PR. 1965. E-Galactosidase of Streptococcus lactis. J Bacteriol, 89 (4), 937-942.
  • Li J, Zhang W, Wang C, Yu Q, Dai R, Pei X. 2012. Lactococcus lactis expressing food-grade E-galactosidase alleviates lactose intolerance symptoms in post-weaning Balb/c mice. Appl Microbiol Biotechnol, 96 (6), 1499-506.
  • Jokar A, Karbassi A. 2011. In-house production of lactose-hydrolysed milk by beta-galactosidase from Lactobacillus bulgaricus. J Agr Sci Technol, 13 (4), 577-584.
  • Almeida CC, Lorena SLS, Pavan CR, Akasaka HMI, Mesquita MA. 2012. Beneficial effects on long-term consumption of a probiotic combination of Lactobacillus casei Shirota and Bifidobacterium breveYakult may persist after suspension of therapy in lactose- intolerant patients. Nutr Clin Pract, 27 (2), 247-251.
  • Ouwehand AC, Salminen S, Isolauri E. 2002. Probiotics: An overview of beneficial effects. Antonie Leeuwenhoek, 82 (1-4), 279-289.
  • Tari C, Ustok FI, Harsa S. 2010. Production of food grade E-galactosidase from artisanal yogurt strains. Food Biotechnol, 24 (1), 78-94.
  • Mozumder NHMR, Akhtaruzzaman M, Bakr MA, Zohra FT. 2012. Study on isolation and partial purification of lactase (E-Galactosidase) enzyme from Lactobacillus bacteria isolated from yogurt. J Sci Res, 4 (1), 239-249.
  • Meira MMS, Helfer EV, Velho VR, Lopes CF, Brandelli A. 2012. Probiotic potential of Lactobacillus spp. isolated from Brazilian regional ovine cheese. J Dairy Res, 79 (1), 119-127.
  • Hsu CA, Yu RC, Chou CC. 2005. Production of E-galactosidase by Bifidobacteria as influenced by various culture conditions. Int J Food Microbiol, 104 (2), 197-206.
  • Toba T, Tomita Y, Itoh T, Adachi S. 1981. E-Galactosidases of lactic acid bacteria: characterization by oligosaccharides formed during hydrolysis of lactose. J Dairy Sci, 64 (2), 185-192.
  • Üstok FI. 2007. Production of E-Galactosidase Using Lactic Acid Bacteria and Optimisation of Fermentation Parameters. (M.Sc), Fen Bilimleri Enstitüsü, ‹zmir ‹leri teknoloji Enstitüsü, ‹zmir, 35s.
  • Iqbal S. 2011. Characterization of E-Galactosidase and Enzymatic Synthesis of Prebiotic Galacto-oligosaccharides. M.Sc. Hons., University of Natural Resources and Life Sciences, Vienna, 70 p.
  • Kim JW, Rajagopal SN. 2000. Isolation and characterization of E-galactosidase from Lactobacillus crispatus. Folia Microbiol, 45, 29-34. 29. Pamuk F. 2011. Biyokimya, Gazi Kitabevi, Ankara, Türkiye, 272s.
  • Tzortzis G, Goulas AK, Gibson GR. 2005. Synthesis of prebiotic galactooligosaccharides using whole cells of a noval strain Bifidobacterium bifidumNCIMB 41171. Appl Microbiol Biotechnol, 68 (3), 412-416.
  • Rabiu BA, Jay AJ, Gibson GR, Rastall RA. 2001. Synthesis and fermentation properties of novel galacto-oligosaccharides by E-galactosidase from Bifidobacterium species. Appl Environ Microbiol, 67 (6), 2526-2530.

Lactobacillus ve Bifidobacterium Cinsi Bakterilerin Beta Galaktosidaz Enzim Aktivitelerinin Belirlenmesi

Yıl 2014, Cilt: 39 Sayı: 4, 211 - 218, 01.08.2014

Öz

Bu çalışmada, insan, gıda ve hayvan kaynaklı 39 Lactobacilllus cinsine ait ve yeni doğan gaitasından izole edilmiş 3 Bifidobacterium cinsine ait toplam 42 bakteri kullanılmıştır. O-nitrofenil-beta-D-galaktosit (o-NPG) substrat olarak kullanılarak, kültürlerin β-galaktosidaz enzim ve spesifik aktiviteleri belirlenmiştir. Lactobacillus cinsine ait kültürlerden L. fermentum ZYN17 (2.468 U/mg), L. casei LB65 (1.116 U/mg), L. rhamnosus GD11 (1.034 U/mg) ve L. acidophilus BAZ36 (0.947 U/mg) suşlarının, Bifidobacterium cinsine ait kültürlerden de B. breve A26 (0.726 U/mg) suşunun en yüksek spesifik aktivite yeteneğine sahip oldukları tespit edilmiştir. Ayrıca, bakterilerin 5-brom-4-klor-3-indolil-β-D-galaktopiranosit (X-gal) substrat bileşiğiyle de nitel olarak enzim aktivitesinin varlığı değerlendirilmiştir. Yüksek spesifik β-galaktozidaz aktivitesi gösteren ZYN17 suşuna ait β-galaktozidaz enziminin optimizasyonu yapılmıştır. β-galaktozidaz enziminin optimum pH’sı 6.8, optimum sıcaklığı 37 ºC ve optimum tamponun potasyum fosfat tamponu olduğu belirlenmiştir.

Kaynakça

  • Gheytanchi E, Heshmati F, Shargh KB, Nowroozi J, Movahedzadeh F. 2010. Study on E-galactosidase enzyme produced by isolated lactobacilli from milk and cheese. Afr J Microbiol Res, 4, 454-458.
  • Karasova P, Spiwok V, Mala S, Kralova B, Russell NJ. 2002. Beta-galactosidase activity in psychrophic microorganisms and their potential use in food industry. Czech J Food Sci, 20(2), 43-47.
  • Verma LM, Barrow JC, Kennedy JF, Puri M. 2012. Immobilization of E-d-galactosidase from Kluyveromyces lactison functionalized silicon dioxide nanoparticles: Characterization and lactose hydrolysis. Int J Biol Macromol, 50(2), 432-437.
  • Panesar R, Panesar PS, Singh RS, Kennedy JF. 2011. Hydrolysis of milk lactose in a packed bed reactor system using immobilized yeast cells. J Chem Technol Biotechnol, 86(1), 42-46.
  • Vonk RJ, Reckman GAR, Harmsen HJM, Priebe MG. 2012. Probiotics and Lactose Intolerance. Chapter 7. InTech Open, 7, 149-160.
  • Singh VP, Sharma J, Babu S, Rizwanulla, Singla A. 2013. Role of probiotics in health and disease: a review. J Pak Med Assoc, 63(2), 253-257.
  • Setty-Shah N, Maranda L, Candela N, Fong J, Dahod I, Rogol AD, Nwosu BU. 2013. Lactose Intolerance: Lack of Evidence for Short Stature or Vitamin D Deficiency in Prepubertal Children. PLOS ONE, 8, 1-9.
  • Bayhan A, Yentür G. 1993. Laktoz ‹ntoleransı. GIDA, 18 (6), 385-388.
  • Rolfe RD. 2000. The role of probiotic cultures in the control of gastrointestinal health. J Nutr, (Supplement).130 (2), 396-402.
  • Sanders ME. 1999. Probiotics. Food Technol, 53 (11), 67-77.
  • De Vrese M, Stegelmann A, Richter B, Fenselau S, Laue C, Schrezenmeir J. 2001. Probiotics- compensation for lactase insufficiency. Am J Clin Nutr, 73 (2), 421-429.
  • Zhang W, Wang C, Huang CY, Yu Q, Liu HC, Zhang CW, Pei XF, Xu X, Wang GQ. 2012. Analysis of E-galactosidase production and their genes of two strains of Lactobacillus bulgaricus. Biotechnol Lett, 34 (6), 1067-1071.
  • Shah NP, Otieno DO. 2007. Endogenous E-glucosidase and E-galactosidase activities from selected probiotic microorganisms and their role in isoflavone biotransformation in soymilk. J Appl Microbiol, 103 (4), 910-917.
  • Kara F. 2004. Release and characterization of beta-galactosidase from Lactobacillus plantarum. M.C. Thesis, Department of Biotechnology, Middle East Technical University, 89p.
  • Ismail SAA, El-Mohamady Y, Helmy WA, Abou-Romia R, Hashem AM. 2010. Cultural condition affecting the growth and production of E-galactosidase by Lactobacillus acidophilus NRRL 4495. Aust J Basic Appl Sci, 4 (10), 5051-5058.
  • Citti JE, Sandine WE, Elliker PR. 1965. E-Galactosidase of Streptococcus lactis. J Bacteriol, 89 (4), 937-942.
  • Li J, Zhang W, Wang C, Yu Q, Dai R, Pei X. 2012. Lactococcus lactis expressing food-grade E-galactosidase alleviates lactose intolerance symptoms in post-weaning Balb/c mice. Appl Microbiol Biotechnol, 96 (6), 1499-506.
  • Jokar A, Karbassi A. 2011. In-house production of lactose-hydrolysed milk by beta-galactosidase from Lactobacillus bulgaricus. J Agr Sci Technol, 13 (4), 577-584.
  • Almeida CC, Lorena SLS, Pavan CR, Akasaka HMI, Mesquita MA. 2012. Beneficial effects on long-term consumption of a probiotic combination of Lactobacillus casei Shirota and Bifidobacterium breveYakult may persist after suspension of therapy in lactose- intolerant patients. Nutr Clin Pract, 27 (2), 247-251.
  • Ouwehand AC, Salminen S, Isolauri E. 2002. Probiotics: An overview of beneficial effects. Antonie Leeuwenhoek, 82 (1-4), 279-289.
  • Tari C, Ustok FI, Harsa S. 2010. Production of food grade E-galactosidase from artisanal yogurt strains. Food Biotechnol, 24 (1), 78-94.
  • Mozumder NHMR, Akhtaruzzaman M, Bakr MA, Zohra FT. 2012. Study on isolation and partial purification of lactase (E-Galactosidase) enzyme from Lactobacillus bacteria isolated from yogurt. J Sci Res, 4 (1), 239-249.
  • Meira MMS, Helfer EV, Velho VR, Lopes CF, Brandelli A. 2012. Probiotic potential of Lactobacillus spp. isolated from Brazilian regional ovine cheese. J Dairy Res, 79 (1), 119-127.
  • Hsu CA, Yu RC, Chou CC. 2005. Production of E-galactosidase by Bifidobacteria as influenced by various culture conditions. Int J Food Microbiol, 104 (2), 197-206.
  • Toba T, Tomita Y, Itoh T, Adachi S. 1981. E-Galactosidases of lactic acid bacteria: characterization by oligosaccharides formed during hydrolysis of lactose. J Dairy Sci, 64 (2), 185-192.
  • Üstok FI. 2007. Production of E-Galactosidase Using Lactic Acid Bacteria and Optimisation of Fermentation Parameters. (M.Sc), Fen Bilimleri Enstitüsü, ‹zmir ‹leri teknoloji Enstitüsü, ‹zmir, 35s.
  • Iqbal S. 2011. Characterization of E-Galactosidase and Enzymatic Synthesis of Prebiotic Galacto-oligosaccharides. M.Sc. Hons., University of Natural Resources and Life Sciences, Vienna, 70 p.
  • Kim JW, Rajagopal SN. 2000. Isolation and characterization of E-galactosidase from Lactobacillus crispatus. Folia Microbiol, 45, 29-34. 29. Pamuk F. 2011. Biyokimya, Gazi Kitabevi, Ankara, Türkiye, 272s.
  • Tzortzis G, Goulas AK, Gibson GR. 2005. Synthesis of prebiotic galactooligosaccharides using whole cells of a noval strain Bifidobacterium bifidumNCIMB 41171. Appl Microbiol Biotechnol, 68 (3), 412-416.
  • Rabiu BA, Jay AJ, Gibson GR, Rastall RA. 2001. Synthesis and fermentation properties of novel galacto-oligosaccharides by E-galactosidase from Bifidobacterium species. Appl Environ Microbiol, 67 (6), 2526-2530.
Toplam 30 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Bölüm Makaleler
Yazarlar

Yasemin Kılıç Bu kişi benim

Zehra Nur Yüksekdağ Bu kişi benim

Hazer Yüksekdağ Bu kişi benim

Yayımlanma Tarihi 1 Ağustos 2014
Yayımlandığı Sayı Yıl 2014 Cilt: 39 Sayı: 4

Kaynak Göster

APA Kılıç, Y. ., Yüksekdağ, Z. N. ., & Yüksekdağ, H. . (2014). Lactobacillus ve Bifidobacterium Cinsi Bakterilerin Beta Galaktosidaz Enzim Aktivitelerinin Belirlenmesi. Gıda, 39(4), 211-218.
AMA Kılıç Y, Yüksekdağ ZN, Yüksekdağ H. Lactobacillus ve Bifidobacterium Cinsi Bakterilerin Beta Galaktosidaz Enzim Aktivitelerinin Belirlenmesi. GIDA. Ağustos 2014;39(4):211-218.
Chicago Kılıç, Yasemin, Zehra Nur Yüksekdağ, ve Hazer Yüksekdağ. “Lactobacillus Ve Bifidobacterium Cinsi Bakterilerin Beta Galaktosidaz Enzim Aktivitelerinin Belirlenmesi”. Gıda 39, sy. 4 (Ağustos 2014): 211-18.
EndNote Kılıç Y, Yüksekdağ ZN, Yüksekdağ H (01 Ağustos 2014) Lactobacillus ve Bifidobacterium Cinsi Bakterilerin Beta Galaktosidaz Enzim Aktivitelerinin Belirlenmesi. Gıda 39 4 211–218.
IEEE Y. . Kılıç, Z. N. . Yüksekdağ, ve H. . Yüksekdağ, “Lactobacillus ve Bifidobacterium Cinsi Bakterilerin Beta Galaktosidaz Enzim Aktivitelerinin Belirlenmesi”, GIDA, c. 39, sy. 4, ss. 211–218, 2014.
ISNAD Kılıç, Yasemin vd. “Lactobacillus Ve Bifidobacterium Cinsi Bakterilerin Beta Galaktosidaz Enzim Aktivitelerinin Belirlenmesi”. Gıda 39/4 (Ağustos 2014), 211-218.
JAMA Kılıç Y, Yüksekdağ ZN, Yüksekdağ H. Lactobacillus ve Bifidobacterium Cinsi Bakterilerin Beta Galaktosidaz Enzim Aktivitelerinin Belirlenmesi. GIDA. 2014;39:211–218.
MLA Kılıç, Yasemin vd. “Lactobacillus Ve Bifidobacterium Cinsi Bakterilerin Beta Galaktosidaz Enzim Aktivitelerinin Belirlenmesi”. Gıda, c. 39, sy. 4, 2014, ss. 211-8.
Vancouver Kılıç Y, Yüksekdağ ZN, Yüksekdağ H. Lactobacillus ve Bifidobacterium Cinsi Bakterilerin Beta Galaktosidaz Enzim Aktivitelerinin Belirlenmesi. GIDA. 2014;39(4):211-8.

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