Glüten Analizinde HPLC, LC-MS/MS Yöntemlerinin ELISA ile Karşılaştırılması

Yıl 2020, Sayı: 24, 30 - 54, 30.08.2020

Ali Özcan İsmail Azar Arzu Yavuz Hakan Yavaş Emre Tokat Vesile Çetin

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Amaç: Tahıl ve ürünleri içerdikleri Glüten nedeni ile bazı insanlarda rahatsızlıklara neden olabilmektedirler. Bu hastalıklardan biri olan çölyak, buğday, çavdar, arpa ve bazen de yulaf ürünlerinin tüketimi sonucu bağırsakta ortaya çıkan bir hastalıktır. Hastalığın nedenini oluşturan temel etken glüten proteininin gliadin adlı alt
fraksiyonudur. Hastalığa sebep olan glüten fraksiyonları glütenin ve gliadinlerdir. Glütenin kendi içerisinde ω5, LMW ve HMW olmak üzere üç alt fraksiyona ayrılmaktadır. Gliadinler ise α(Alfa), ω(Omega), β(Beta) ve γ(Gamma) olmak üzere dört fraksiyondan oluşmaktadır.

Materyal ve Yöntem: Bu çalışmada çölyak hastalarına özel olarak üretilen gıdaların analizlerinde kullanılabilecek ve yasal limitler olan 0-20 mg/kg ile 20-80 mg/kg aralıklarında LOD değerine sahip kromatografik bir yöntem geliştirilmeye çalışılmıştır.

Bulgular ve Sonuç: Yapılan HPLC çalışmalarında LOD değeri 31,94mg/kg, LOQ değeri ise 106,47mg/kg düzeylerinde metot performansları elde edilmiştir. LC MS/MS çalışmalarında elde edilen LOD değeri 19,24 mg/kg, LOQ değeri ise 64,13 mg/kg olarak tespit edilmiştir.

Anahtar Kelimeler

Çölyak Hastalığı,, ELISA,, HPLC, Glüten, LC-MS/MS, Deliac Disase,

Comparison of HPLC, LC-MS / MS Methods with ELISA in Gluten Analysis

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Abstract

Objective: Cereals and products may cause disorder in some people due to the Gluten they contain. Celiac, one of these diseases, is a disease that occurs in the intestine as a result of consumption of wheat, rye, barley and sometimes oat products. The main cause of the disease is the sub-fraction of gluten protein called gliadin. The gluten fractions that cause disease are glüten and gliadins. Gluten is divided into three sub-fractions: ω5, LMW and HMW. Gliadins consist of four fractions: α (Alpha), ω (Omega), β (Beta) and γ (Gamma).

Marerials and Methods: In this study, it was tried to develop a chromatographic method that can be used in the analysis of foods specially produced for celiac patients and has an LOD value between 0-20 mg / kg and 20-80 mg / kg, which are legal limits.

Result and Conclusion: In the HPLC studies, the method performances were obtained at the LOD value of 31,94 mg/kg and the LOQ value at 106,47 mg/kg. LOD value obtained in LC MS/MS studies was determined 19,24mg / kg and LOQ value 64,13 mg / kg.

Anahtar Kelimeler

Celiac disase,, Gluten, HPLC, ELISA, LC-MS/MS

Kaynakça

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