Effect of Rhizobacteria Isolated from the Rhizosphere of Solanum lycopersicum L. on Tomato Seed Germination

Öz

Plant growth-promoting rhizobacteria (PGPR) are a group of bacteria that colonize plant roots without causing any damage and contribute to seed germination and plant development through mechanisms such as indole-3-acetic acid (IAA) production and phosphate solubilization. 10 isolates from the rhizosphere of Solanum lycopersicum L. (Ayas tomatoes) were identified using MALDI-TOF-MS to investigate morphology, biochemistry, and plant growth-promoting traits (phosphate solubilization and IAA production) in this study. Among all isolates, four (DMTS-2, DMTS-4, DMTS-6, DMTS-10) produced IAA, while five (DMTS-2, DMTS-4, DMTS-6, DMTS-7, DMTS-9) solubilized inorganic phosphate. Three isolates (DMTS-2, DMTS-4, and DMTS-6), solubilized both inorganic phosphate and produced IAA. They were selected for germination testing in this study. Furthermore, three rhizospheric bacterial isolates (DMTS-2, DMTS-4, and DMTS-6) were inoculated onto tomato seeds and monitored in a Petri dish assay at 25 °C for 14 days. The experiment was conducted using a randomized block design with three replicates, each comprising 20 seeds. The results demonstrated that the three selected indigenous rhizobacterial treatments had a significantly positive effect on germination percentage, vigor index, and seedling growth parameters over 14 days compared with the untreated control. The highest vigor index and germination percentage increases were observed in isolate DMTS-4, with a vigor index of 425.1 and a germination percentage of 65.4%. This study clearly demonstrated that the selected local isolates (DMTS-2, DMTS-4, and DMTS-6) enhanced the germination success of Solanum lycopersicum through their abilities to produce IAA and solubilize inorganic phosphate.

Anahtar Kelimeler

• Growth-promoting
• Microbial fertilizers
• PGPR
• Pseudomonas chlororaphis

Etik Beyan

There is no need to obtain ethics committee approval for this study.

Solanum lycopersicum L. rizosferinden izole edilen rizobakterilerin domates tohum çimlenmesine üzerine etkisi

Öz

Bitki gelişimini teşvik eden rizobakteriler (PGPR), IAA üretimi ve fosfat çözünürlüğü gibi çeşitli mekanizmalar aracılığıyla bitki gelişimini destekler. Bu çalışmada, Solanum lycopersicum L. (Ayaş domatesi) rizosferinden izole edilen 10 izolat MALDI-TOF-MS methodu ile tanımlanarak morfolojik, biyokimyasal ve bitki büyümesini teşvik eden özellikleri (fosfat çözünürlüğü ve IAA üretimi) açısından değerlendirildi. Domates rizosferinden izole edilen 10 izolattan dört tanesinin (DMTS-2, DMTS-4, DMTS-6 ve DMTS-10) IAA ürettiği ve beş izolatın (DMTS-2, DMTS-4, DMTS-6, DMTS-7 ve DMTS-9) inorganik fosfatı çözebildiği, üç izolatın ise (DMTS-2, DMTS-4 ve DMTS-6) hem inorganik fosfatı çözdüğü hem de IAA ürettiği belirlendi ve bu çalışmada çimlenme testi için seçildiler. Ayrıca, bu üç rizosferik bakteri izolatı (DMTS-2, DMTS-4 ve DMTS-6) domates tohumlarına aşılandı ve 25 °C’de 14 gün boyunca Petri kabı deneyi ile izlendi. Deney, her biri 20 tohumdan oluşan üç tekrar ile tesadüf parselleri deneme desenine göre yürütüldü. Sonuçlar, çimlenme testi için seçilen üç yerel rizobakteriyel uygulamanın (DMTS-2, DMTS-4 ve DMTS-6), 14 günlük süreç boyunca, kontrol grubuna kıyasla çimlenme yüzdesi, vigor indeksi ve fide büyüme parametreleri üzerinde olumlu bir etkiye sahip olduğunu gösterdi. En yüksek vigor indeksi ve çimlenme yüzdesi artışları, sırasıyla 425,1 vigor indeksi değeri ve %65,4 çimlenme yüzdesi ile DMTS-4 izolatında gözlemlendi. Bu çalışma, seçilen yerel izolatların (DMTS-2, DMTS-4 ve DMTS-6) IAA üretme ve inorganik fosfatı çözündürme yetenekleri sayesinde Solanum lycopersicum'un çimlenme başarısını artırdığını açıkça göstermiştir.

Anahtar Kelimeler

• Büyümeyi destekleyici
• Mikrobiyal gübre
• PGPR
• Pseudomonas chlororaphis

Kaynakça

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