Samsun, Sinop ve Bolu İllerindeki Bal Kabağı (Cucurbita Moschata Duch) Popülasyonlarına Ait Tohum Örneklerinde Virüslerin Tanılanması ve Bulunma Durumlarının Belirlenmesi

Öz

Bu çalışma Samsun, Sinop ve Bolu illerinden toplanan bal kabağı populasyonlarına ait tohum örneklerinde tohum kaynaklı bazı virüslerin belirlenmesi amacıyla 2013-2014 yıllarında yürütülmüştür. Tohum örnekleri Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Squash mosaic virus (SqMV), Tobacco ring spot virus (TRSV) ve Cucumber green mottle mosaic virus (CGMMV) için Double Sandwich-Enzyme linked Immunosorbent Assay (DAS-ELISA) yöntemi ile analiz edilmiştir. Elde edilen sonuçlara göre, tohum örneklerinin sadece ZYMV ve CMV ile enfekteli olduğu belirlenmiş ve bal kabağı tohum örneklerinde ZYMV ve CMV adlı etmenlerin bulunma oranlarının sırasıyla %12.5 ve %4.1 olduğu saptanmıştır. SqMV, TRSV ve CGMMV ile enfekteli olan hiçbir bal kabağı tohum örneğine rastlanmamıştır. Çalışma sonucunda, Karadeniz Bölgesi’nde bal kabağı populasyonlarına ait tohum örneklerinde ilk kez tohum kaynaklı virüslerin tanılanması yapılmış ve bulunma durumları belirlenmiştir.

Anahtar Kelimeler

• Bal kabağı
• Karadeniz Bölgesi
• tanılama
• tohum
• virüs

The Detection and Occurrence of Viruses on the Seed Lots of Pumpkin (Cucurbita Moschata Duch) Populations in Samsun, Sinop, and Bolu Provinces

Öz

This study was carried out to detect the presence of seed borne viruses in pumpkin seed lots collected from Samsun, Sinop, and Bolu provinces during 2013-2014. The seed samples were tested by Double sandwich-enzyme linked immunosorbent assay (DAS-ELISA) for Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Squash mosaic virus (SqMV), Tobacco ring spot virus (TRSV), and Cucumber green mottle mosaic virus (CGMMV). Based on the results, the seed samples were only infected with ZYMV and CMV. Infection ratio for ZYMV and CMV was determined as 12.5% and 4.1%, respectively. Moreover, none of the seed samples were found to be infected for SqMV, TRSV, and CGMMV. This is the first time seed-borne viruses of pumpkin populations in the Black Sea Region of Turkey have been identified and their prevalence was determined.

Anahtar Kelimeler

• Pumpkin
• The Black Sea Region
• identification
• seed
• virus

Kaynakça

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