Samsun, Sinop ve Bolu İllerindeki Bal Kabağı (Cucurbita Moschata Duch) Popülasyonlarına Ait Tohum Örneklerinde Virüslerin Tanılanması ve Bulunma Durumlarının Belirlenmesi

Yıl 2015, , 70 - 77, 18.01.2016

Mehmet Ali Şevik Ahmet Balkaya

https://doi.org/10.13002/jafag873

Cited By: 5

Öz

Bu çalışma Samsun, Sinop ve Bolu illerinden toplanan bal kabağı populasyonlarına ait tohum örneklerinde tohum kaynaklı bazı virüslerin belirlenmesi amacıyla 2013-2014 yıllarında yürütülmüştür. Tohum örnekleri Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Squash mosaic virus (SqMV), Tobacco ring spot virus (TRSV) ve Cucumber green mottle mosaic virus (CGMMV) için Double Sandwich-Enzyme linked Immunosorbent Assay (DAS-ELISA) yöntemi ile analiz edilmiştir. Elde edilen sonuçlara göre, tohum örneklerinin sadece ZYMV ve CMV ile enfekteli olduğu belirlenmiş ve bal kabağı tohum örneklerinde ZYMV ve CMV adlı etmenlerin bulunma oranlarının sırasıyla %12.5 ve %4.1 olduğu saptanmıştır. SqMV, TRSV ve CGMMV ile enfekteli olan hiçbir bal kabağı tohum örneğine rastlanmamıştır. Çalışma sonucunda, Karadeniz Bölgesi’nde bal kabağı populasyonlarına ait tohum örneklerinde ilk kez tohum kaynaklı virüslerin tanılanması yapılmış ve bulunma durumları belirlenmiştir.

Anahtar Kelimeler

Bal kabağı, Karadeniz Bölgesi, tanılama, tohum, virüs

The Detection and Occurrence of Viruses on the Seed Lots of Pumpkin (Cucurbita Moschata Duch) Populations in Samsun, Sinop, and Bolu Provinces

Öz

This study was carried out to detect the presence of seed borne viruses in pumpkin seed lots collected from Samsun, Sinop, and Bolu provinces during 2013-2014. The seed samples were tested by Double sandwich-enzyme linked immunosorbent assay (DAS-ELISA) for Cucumber mosaic virus (CMV), Zucchini yellow mosaic virus (ZYMV), Squash mosaic virus (SqMV), Tobacco ring spot virus (TRSV), and Cucumber green mottle mosaic virus (CGMMV). Based on the results, the seed samples were only infected with ZYMV and CMV. Infection ratio for ZYMV and CMV was determined as 12.5% and 4.1%, respectively. Moreover, none of the seed samples were found to be infected for SqMV, TRSV, and CGMMV. This is the first time seed-borne viruses of pumpkin populations in the Black Sea Region of Turkey have been identified and their prevalence was determined.

Anahtar Kelimeler

Pumpkin, The Black Sea Region, identification, seed, virus

Kaynakça

• Ali A, Mohammad O and Khattab A (2012). Distribution of viruses infecting cucurbit crops and isolation of potential new virus-like sequences from weeds in Oklahoma. Plant Disease, 96: 243–248.
• Arli-Sokmen M, Mennan H, Sevik MA and Ecevit O (2005). Occurrence of viruses in field-grown pepper crops and some of their reservoir weed hosts in Samsun, Turkey. Phytoparasitica, 33: 347-358.
• Bananej K, Keshavarz T, Vahdat A, Salekdeh GH and Glasa M (2008). Biological and molecular variability of Zucchini yellow mosaic virus in Iran. Journal of Phytopathology, 156: 654–659.
• Bananej K and Vahdat A (2008). Identification, distribution and incidence of viruses in field-grown cucurbit crops of Iran. Phytopathologia Mediterranea, 47: 247–257.
• Balkaya A, Özbakır M ve Karaağaç O (2010). Karadeniz Bölgesinden toplanan balkabağı (Cucurbita moschata Duch.) populasyonlarının karakterizasyonu ve meyve özelliklerindeki varyasyonun değerlendirilmesi. Tarım Bilimleri Dergisi, 16: 17–25.
• Balkaya A, Cankaya S and Ozbakir M (2011). Use of canonical correlation analysis for determination of relationships between plant characters and yield components in winter squash (Cucurbita maxima Duch.) populations. Bulgarian Journal of Agricultural Science, 17: 606–614.
• Bisognin DA (2002). Origin and Evolution of Cultivated Cucurbits. Ciencia Rural, Santa Maria, 32: 715-723.
• Bostan H, Kaymak HC and Haliloglu K (2002). Detection of Cucumber mosaic virus (CMV) and Zucchini yellow mosaic virus (ZYMV) in squash in Erzurum, Erzincan and Artvin provinces by serological and biological methods. Journal of Turkish Phytopathology, 31: 9–14.
• Caglar BK, Guldur ME and Yilmaz MA (2004). First report of Squash mosaic virus in Turkey. Journal of Plant Pathology, 86: 177–180.
• Clark MR and Adams AM (1977). Characteristics of the microplate method of enzyme- linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34: 475–483.
• Çandar A ve Erkan S (2011). Bitkilerde viral etmenlere karsı genetik dayanıklılık mekanizmaları. Elektronik Mikrobiyoloji Dergisi TR, 9: 13–27.
• Çıtır A, Kutluk ND, Sağlam N ve İlbağı H (1998). Amasya, Çorum, Samsun ve Tokat illerinde hıyar ve kabak kültürlerinde görülen virüs hastalıklarının simptomolojik ve biyolojik yöntemlerle tanıları. Türkiye VIII. Fitopatoloji Kongresi, 21–25 Eylül 1998, s. 331–335. Ankara.
• Davis RF and Muzuki MK (1986). Seed transmission of Zucchini yellow mosaic virus in Cucurbita pepo. Proceedings of the Workshop on Epidemiology of Plant Virus Disease, 50–52, Florida, USA.
• Fidan U (1995). Virus diseases of vegetables in greenhouses in İzmir and Muğla. Journal of Turkish Phytopathology, 24: 7–14.
• Fletcher JD, Wallace AR and Rogers BT (2000). Potyviruses in New Zealand butternut squash (Cucurbita maxima Duch.): Yield and quality effects of ZYMV and WMV2 virus infections. New Zealand Journal of Crop and Horticultural Science, 28: 17–26.
• Fuchs M (2013). Prevention and management of viruses in cucurbit crops. New England Vegetable and Fruit Conference, 189–191, Manchester, New Hampshire, UK. Gümüş M, Erkan S ve Tok S (2004). Bazı kabakgil türlerinin tohumlarındaki viral etmenlerin saptanması üzerinde araştırmalar. Ege Üniversitesi Ziraat Fakültesi Dergisi, 41: 49–56.
• Harlan JR (1951). Anatomy of gene centres. The American Naturalist, 85: 97-103.
• Jeffrey (2005). New system of Cucurbitaceae. Botaniske Zhurnal, 90: 332–335.
• Kaper JM and Waterworth HE (1981). Cucumoviruses. in: Handbook of plant virus infection and comparative diagnosis. Edited Kurstak E, Publ. by the Elsevier/North-Holland Biochemical Press, 257–332.
• Kaya A ve Erkan S (2007). İzmir, Aydın, Manisa ve Balıkesir illerinde üretilen kabakgillerdeki viral hastalık etmenlerinin tanılanması. Türkiye II. Bitki Koruma Kongresi, 27–29 Ağustos 2007, s.306. Isparta.
• Koenraadt HMS and Remeeus PM (2010). Detection of Squash mosaic virus, Cucumber green mottle mosaic virus and Melon necrotic spot virus in cucurbits. Seed Health Testing Methods. ISTA Method Validation Reports, 7: 1–8.
• Koklu G and Yilmaz O (2006). Occurrence of cucurbit viruses on field-grown melon and watermelon in the Thrace region of Turkey. Phytoprotection, 87: 123–130. Nee M (1990). The domestication of Cucurbita. Economic Botany, 44: 56–68
• Nontajak S, Vulyasevi S, Jonglaekha N and Smitamana P (2014). Effect of mixed viruses infection on symptom expression in zucchini (Cucurbita pepo L.). Journal of Agricultural Technology, 10: 1329–1341.
• Ozaslan M, Aytekin T, Bas B, Kilic H, Afacan ID, and Dag DS (2006). Virus diseases of Cucurbits in Gaziantep, Turkey. The Plant Pathology Journal, 5: 24–27.
• Pinto ZV, Rezende JAM, Yuki VA and Piedade SMS (2008). Ability of Aphis gossypii and Myzus persicae to transmit Cucumber mosaic virus in single and mixed infection with two potyviruses to zucchini squash. Summa Phytopathologica, 34: 183–185.
• Provvidenti R (1996). Diseases caused by virus. in: Compendium of Cucurbit Diseases. Edited Zitter T A, American Phytopathological Society, St. Paul, MN, 37–45. Sastry KS (2013). Seed-borne plant virus diseases. Springer, New Delhi, India.
• Sertkaya G, Sertkaya E, Yetisir H and Kaya K (2004). Investigations on incidence and transmission of Zucchini yellow mosaic virus (ZYMV) in cucurbits Hatay province. Proceedings of the First Plant Protection Congress of Turkey., 8–10 September 2004, 217, Samsun, Turkey.
• Sevik MA and Arli-Sokmen M (2003). Viruses infecting cucurbits in Samsun, Turkey. Plant Disease, 87: 341–344.
• Sevik MA and Toksoz Y (2008). Occurrence of Squash mosaic virus (SqMV) infecting pumpkin and squash growing in Samsun, Turkey. Journal of Turkish Phytopathology, 37: 15–25.
• Tobias I and Palkovics L (2003). Characterization of Hungarian isolates of Zucchini yellow mosaic virus (ZYMV, potyvirus) transmitted by seeds of Cucurbita pepo var styriaca. Pest Management Science, 59: 493–497.
• TÜİK (2014). Bitkisel Üretim İstatistikleri. http://tuikapp.tuik.gov.tr/bitkiselapp/bitkisel.zul
• Uçar F ve Ertunç F (1998). Antalya ili kabak seralarında görülen Zucchini sarı mozayik virüsünün enfeksiyon kaynaklarının belirlenmesi üzerine araştırmalar. Türkiye VIII. Fitopatoloji Kongresi, 21–25 Eylül 1998, s. 228-233. Ankara.
• Usher L, Sivparsad B and Gubba A (2012). Isolation, identification and molecular characterization of an isolate of Zucchini yellow mosaic virus occurring in KwaZulu-Natal, South Africa. South African Journal of Plant and Soil, 29: 65–71.
• Vargun Z and Ertunc F (1994). Research on interactions of Cucumber mosaic and Zucchini yellow mosaic viruses on squash. 9 th. Congress of the Mediterranean Phytopathological Union, 491–493, Aydın, Turkey.
• Whitaker TW and Bemis WP (1964). Evolotion in genus Cucurbita. Evolution, 18: 553–559.
• Whitaker TW and Bemis WP (1975). Orijin and evolution of the cultivated Cucurbita. Bulletin of the Torrey Botanical Club, 102: 362–368.
• Yılmaz MA, Baloğlu S, Özaslan M ve Güldür ME (1995). GAP Bölgesi kültür bitkilerinde belirlenen virüsler. GAP Bölgesi Bitki Koruma Sorunları ve Çözüm Önerileri Sempozyumu, 27–29 Nisan 1995, s. 241–250. Şanlıurfa.
• Yilmaz MA and Davis RF (1984). Purification and particle morphology of TMV, CMV and ZYMV isolated from various cultivated crops grown along the Mediterranean Coast of Turkey. Journal of Turkish Phytopathology, 13: 20–28.
• Yilmaz MA, Abak K, Lecoq H, Baloglu S, Sari N, Kesici S, Ozaslan M and Guldur ME (1994). Control of Zucchini yellow mosaic virus (ZYMV) in cucurbits by ZYMV-WK strain. 9th Congress of the Mediterranean Phythopathological Union, 353–356. Aydın, Turkey.
• Yorganci U, Erkan S, Ozaktan H and Eser B (1994). Detection of the agents of viral and bacterial disaases in seeds of pepper, temato, eggplant, cucumber and their inactivation ways. 9th Congress of the Mediterranean Phythopathological Union, 37–39. Aydın, Turkey.