Serada Yetiştirilen Domates Bitkilerinde Zararlı Kök-ur Nematodu (Meloidogyne javanica)‘na Karşı Entomopatojen Nematodların ve Simbiyont Bakterilerinin Etkinliği

Yıl 2016, Cilt: 33 Sayı: 1, 162 - 172, 16.05.2016

İlker Kepenekci

https://doi.org/10.13002/jafag909

Öz

Çalışmada, dört entomopatojen nematod türünün (EPN) (Steinernema carpocapsae, S. feltiae, S. glaseri ve Heterorhabditis bacteriophora) ve simbiyotik olarak yaşayan bakterilerinin (Xenorhabdus bovienii ve Photorhabdus luminescens kayaii), serada yetiştirilen domates (SC-2121 çeşidi) bitkilerinde zararlı kök-ur nematodu (Meloidogyne javanica)‘nun yumurta (yumurta açılımına) ve ikinci dönem enfektif larvaları (L2)'na (larvaya toksisite) karşı etkisi araştırılmıştır. Denemeler sonunda; her bir bitki kökündeki M. javanica’nın oluşturduğu yumurta paketi sayısı, bitkinin boyu (cm), bitki ve kökün yaş ve kuru ağırlığı (g) parametreleri değerlendirilmiştir. Çalışmada S. feltiae (sulu süspansiyon ve kadavra uygulamaları) ve X. bovienii etkili bulunmuştur.

Anahtar Kelimeler

Kök-ur nematodları, Meloidogyne javanica, entomopatojen nematodlar, Xenorhabdus, Photorhabdus, enfekteli kadavra

Kaynakça

• Aatif HM, Javed N, Khan SA and Ahmed S (2012). Virulence of Xenorhabdus and Photorhabdus bacteria and their toxins against juvenile’s immobilization of Meloidogyne incognita. Pakistan Journal of Phytopathology, 24: 170-174.
• Akhurst RJ (1982). Antibiotic activity of Xenorhabdus spp., bacteria symbiotically associated with insect pathogenic nematodes of the families Heterorhabditidae and Steinernematidae. Journal of General Microbiology, 128: 3061-3065.
• Anonymous (2010). Ruhsatlı Bitki Koruma Ürünleri, Tarım ve Köyişleri Bakanlığı, Koruma ve Kontrol Genel Müdürlüğü, Pulat Basımevi. 398 sayfa.
• Bird AF and Bird J (1986). Observations on the use of insect parasitic nematodes as a means of biological control on root-knot nematodes. International Journal of Parasitology, 16: 511-516.
• Bulun N, Güneş Ç ve Gözel U (2009). Entomopatojen Nematodların Kök ur Nematodu (Meloidogyne incognita, Tylenchida: Meloidogynidae) Üzerine Etkinliğinin Belirlenmesi, Türkiye III. Bitki Koruma Kongresi, Van, 370.
• Daykin ME and Hussey RS (1985). Staining and histopathological techniques in nematology. In: Barker KR, Carter CC and Sasser JN (Eds), An advanced treatise on Meloidogyne Volume II: Methodology, North Carolina State University Graphics, Raleigh, North Carolina, USA, pp. 39-48.
• Del Valle EE, Lax P, Dueñas JR and Doucet ME (2013). Effects of insect cadavers infected by Heterorhabditis bacteriophora and Steinernema diaprepesi on Meloidogyne incognita parasitism in pepper and summer squash plants. Ciencia e Investigación Agraria, 40: 109-118.
• Fallon DJ, Kaya HK, Gaugler R and Sipes BS (2002). Effects of entomopathogenic nematodes on Meloidogyne javanica on tomatoes and soybeans. Journal of Nematology, 34: 239-245.
• Gouge DH, Otto AA, Schirocki A and Hague NGM (1994). Effects of Steinernematids on the root-knot nematode, Meloidogyne javanica. Tests of Agrochemicals and Cultivars No. 15. Annals of Applied Biology Supplement, 124: 134-135.
• Grewal PS, Lewis EE, Venkatachari S (1999). Allelopathy: A possible mechanism of suppression of plant-parasitic nematodes by entomopathogenic nematodes. Nematology 1: 735-743.
• Grewal PS, Martin WR, Miller RW and Lewis EE (1997). Suppression of plant-parasitic nematode populations in turfgrass by application of entomopathogenic nematodes. Biocontrol Science and Technology, 7: 393-399.
• Gulcu B and Hazir S (2012). An alternative storage method for entomopathogenic nematodes. Turkish Journal of Zoology, 36: 562-565.
• Gulcu B, Hazir S and Kaya HK (2012). Scavenger deterrent factor (SDF) from symbiotic bacteria of entomopathogenic nematodes. Journal of Invertebrate Pathology, 110: 326-333.
• Haydak MH (1936). "A food for rearing laboratory insect". Journal of Economic Entomology, 29: 1026.
• Hussey RS and Barker KR (1973). A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter, 57: 1025-1028.
• Ishibashi N and Kondo E (1986). Steinernema feltiae (DD-136) and S. glaseri: Persistence in soil and bark compost and their influence on native nematodes. Journal of Nematology, 18: 310-316.
• Kepenekci İ (2014a). Nematolojik Çalışmalar için Arazi ve Laboratuar Uygulama Kılavuzu, Nematoloji El Kitabı. ISBN: 978-605-4627-71-9, Siyasal Kitabevi, XXII+455 sayfa.
• Kepenekci İ (2014b). Entomopathogenic nematodes (Steinernematidae, Heterorhabditidae: Rhabditida) of Turkey. Pakistan Journal of Nematology, 32: 59-65.
• Khan MR, Mehboob A and Khan U (2010). Interaction of the entomopathogenic nematode Steinernema masoodi and the root-knot nematode Meloidogyne incognita on tomato. Nematologia Mediterranea, 38: 179-185.
• Lamondia JA and Cowles RS (2002). Effect of entomopathogenic nematodes and Trichoderma harzianum on the strawberry black root rot pathogens Pratylenchus penetrans and Rhizoctonia fragariae. Journal of Nematology, 34: 351-357.
• Lewis EE and Grewal PS (2005). Interactions with plantparasitic nematodes. In: PS Grewal, R-U Ehlers and DI Shapiro-Ilan (Eds) Nematodes as biocontrol agents. New York: CABI, pp. 349-362.
• Lewis EE, Grewal PS and Sardanelli S (2001). Interactions between the Steinernema feltiae-Xenorhabdus bovienii insect pathogen complex and the root-knot nematode Meloidogyne incognita. Biological Control, 21: 55-62.
• Melakeberhan H (1997). Effect of temperature and nitrojen source on tomato genotypes responce to Meloidogyne incognita infection. Fundamental and Applied Nematology, 20: 1-8.
• Mohamed MA and Coppel HC (1983). Mass rearing of the greater wax moth Galleria mellonella (Lepidoptera: Pyralidae) for small-scale laboratory studies. Great Lakes Entomologist, 16: 139-141.
• Mohammad AB, Eskandar Z, Saeid S, Mohammad J and Fariba M (2007). Evaluation of sulfosulfuran for broadleaved and grass weed control in wheat (L.) in Iran. Crop Protection, 26: 1385-1389.
• Molina JP, Dolinski C, Souza RM and Lewis EE (2007). Effect of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) on Meloidogyne mayaguensis Rammah and Hirschmann (Tylenchida: Meloidoginidae) infection in tomato plants. Journal of Nematology, 39: 338-342.
• Nakasone KK, Peterson SW and Jong SC (2004). Preservation and Distribution of Fungal Cultures. In: GM Mueller, GF Bills and MS Foster (Eds), Biodiversity of fungi, Inventory and Monitoring Methods. Elsevier Academic Press, pp. 47-37.
• Netscher C and Sikora RA (1990). Nematode parasites of vegetables. In: M Luc, RA Sikora and J Bridge (Eds), Plant Parasitic nematodes in Subtropical and Tropical Agriculture. CAB International, Wellingford, U.K., pp. 237-284.
• Nyczepir A, Shapiro-Ilan DI, Lewis EE and Handoo Z (2004). Effect of entomopathogenic nematodes on Mesocriconema xenoplax populations in peach and pecan. Journal of Nematology 36: 181-185
• Perez EE and Lewis EE (2002). Use of entomopathogenic nematodes to suppress Meloidogyne incognita on greenhouse tomatoes. Journal of Nematology, 34: 171-174.
• Perez EE and Lewis EE (2004). Suppression of Meloidogyne incognita and Meloidogyne hapla with entomopathogenic nematodes on greenhouse peanuts and tomatoes. Biological Control, 30: 336-341.
• Perry RN, Hominick WM, Beane J and Briscoe B (1998). Effect of entomopathogenic nematodes, Steinernema feltiae and S. carpocapsae, on the potato cyst nematode, Globodera rostochiensis, in pot trials. Biocontrol Science and Technology, 8: 175-180.
• Riegel C, Dickson DW, Nguyen KB and Smart GC (1998). Management of root-knot nematodes with entomopathogenic nematodes. Journal of Nematology Supplement, 24: 637-641.
• Shapiro-Ilan DI, Nyczepir A and Lewis EE (2006). Entomopathogenic nematodes and bacteria applications for control of the pecan root-knot nematode, Meloidogyne partityla, in the greenhouse. Journal of Nematology, 38: 449-454.
• Siddiqi MR (1986). Tylenchida parasites of plants and insects. Farnham Royal, UK: Commonwealth Agricultural Bureaux, 645 pp.
• Smith D and Onions AHS (1994). The Preservation and Maintenance of Living Fungi. CAB International Bakeham Lane Egham-England,122 pp.
• Smitley DR, Warner FW and Bird GW (1992). Influence of irrigation and Heterorhabditis bacteriophora on plant-parasitic nematodes in turf. Journal of Nematology Supplement, 24: 637-641.
• Somvanshi VS, Lang E, Straubler B, Sproer C, Schumann P, Ganguly S, Saxena AK and Stackebrandt E (2006). Providencia vermicola sp. nov., isolated from infective juveniles of the entomopathogenic nematode Steinernema thermophilum. International Journal of Systematic and Evolutionary Microbiology, 56: 629-633.
• SPSS (1999). SPSS for Windows, release 10.0.1. Chicago, IL, USA, SPSS.
• Stirling GR (1991). Biological Control of Plant-Parasitic Nematodes. CAB International, Wallingford, Oxon, pp. 50-85.
• Thomas HA and Poinar GO (1979). Xenorhabdus gen. Nov. A genus of entomopathogenic, nematophilic bacteria of teh family enterobacteriaceae int. International Journal of Systematic Bacteriology, 29: 352-360.
• White GF (1927). A method for obtaining infective nematode larvae from cultures. Science, 66: 302-303.
• Whitehead AG (1998). Plant Nematode Control. CAB. International, New York, USA, pp. 209-236.
• Woodring JL and Kaya HK (1988). Steinernematid and Heterorhabditid Nematodes. Arkansas Agricultural Experiment Station, Fayetteville, A.K., A hand Book of Techniques Southern Cooperative Series Bulletin, 331, 30 p.