Methidathion İnsektisit/Akarisitinin Sitotoksik ve Genotoksik Potansiyelinin Allium Testi ile İncelenmesi

Yıl 2020, , 1 - 12, 30.12.2020

Fatma Ünal Nesrin Durdu Helvacı Tülek Deniz Yüzbaşıoğlu , Mustafa Çelik

https://doi.org/10.5281/zenodo.4317924

Öz

Organik fosforlu pestisitlerden olan Methidathion (Supracide 40 EC, MET), meyve ağaçlarında, sebzelerde,
tütünde, yoncada, ayçiçeğinde, seralarda ve gül bahçelerinde çeşitli böcek ve akarlara karşı
kullanılmaktadır. Bu araştırmada, Allium testi kullanılarak, methidathionun Allium cepa kök ucu
hücrelerindeki sitotoksik ve genotoksik etkileri incelenmiştir. Kök büyüme inhibisyon testi ile, etkili
konsantrasyon (EC50) değeri 30 mg/L olarak tespit edildikten sonra, Allium cepa kök uçları, MET’un dört
farklı konsantrasyonu (7,5, 15, 30 ve 45 mg/L) ile 12, 24 ve 48 saat muamele edilmiştir. MET, mitotik indeksi
(MI), tüm konsantrasyonlarda ve uygulama sürelerinde, kontrole kıyasla anlamlı şekilde düşürmüştür. Diğer
yandan MET, kromozomal anormallikleri, kontrole kıyasla anlamlı düzeyde artırmıştır. En yaygın görülen
anormallikler kroımozom yapışıklığı (%47,50) ve C-mitoz (%44,24)’dur. Bunları sırasıyla fragment (%2,75),
köprü (%2,55), geri kalma (%1,63) ve çok kutupluluk (%1,33) takip etmiştir. Çalışmada ayrıca, ön işleme
tabi tutulmuş kök uçlarında kromozom ve kromatid kırıkları, fragmentler ve poliploidi tespit edilmiştir. Bu
çalışmadan elde edilen sonuçlar, MET’un Allium cepa'da belirgin şekilde sitotoksik ve genotoksik olduğunu
göstermiştir. Ayrıca, Allium testinin, MET’un toksik etkilerini belirlemede çok hassas ve etkili bir test
olduğunu doğrulamıştır.

Anahtar Kelimeler

Pestisit, Methidathion, Sitotoksik etki, Genotoksik etki, Allium cepa

Kaynakça

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