Lactobacillus Cinsi Bakteriler Tarafından Üretilen Biyosürfektanların Anti-Biyofilm ve Anti-Mikrobiyal Aktivitelerinin Belirlenmesi

Feride Efe; Zehranur Yuksekdag; Berat Çınar Acar

Lactobacillus Cinsi Bakteriler Tarafından Üretilen Biyosürfektanların Anti-Biyofilm ve Anti-Mikrobiyal Aktivitelerinin Belirlenmesi

Öz

Öz Bu çalışmada, 20 Lactobacillus sp. tarafından üretilen biyosürfektanlar ile Staphylococcus aureus suşlarının biyofilm oluşumunun engellemesi ve/veya azaltılması ve antimikrobiyal aktivitelerinin belirlenmesi amaçlanmıştır. Öncelikle 52 S. aureus suşlarının Kongo Kırmızısı katı besi yerinde biyofilm yapma yetenekleri nitel olarak belirlenmiştir. Bakterilerin Kongo Kırmızısı katı besi yerinde siyah renkte koloniler oluşturması biyofilm pozitif, pembemsi renkte koloniler oluşturması ise biyofilm negatif olarak değerlendirilmiştir. 52 S. aureus suş arasından sadece yedisinin (26, 36, 38, 43, 55, 56, 60) çok kuvvetli (+++) biyofilm oluşturma yeteneğine sahip olduğu belirlenmiş ve çalışmalarda bu bakteriler kullanılmıştır. 20 Lactobacillus sp. suş arasından sekizinin (Lactobacillus acidophilus BAZ 22, L. acidophilus BAZ 51, L. acidophilus BAZ 61, L. brevis YG 7, L. rhamnosus MP 1, L. rhamnosus BAZ 78, Limosilactobacillus fermentum FKK 3 ve L. fermentum LB 16) ürettiği biyosürfektan yedi S. aureus suşun oluşturduğu biyofilmi %0,90-%80,60 arasında değişen oranlarda engellemiştir. En fazla engelleyici etki %80,60 oranı ile S. aureus 26 suşuna karşı L. rhamnosus MP1 suşunun ürettiği biyosürfektanda gözlemlenmiştir. Ancak yapılan çalışmada, laktobasiller tarafından üretilen biyosürfektanların S. aureus’lara karşı anti-mikrobiyal aktivite göstermediği belirlenmiştir.

Anahtar Kelimeler

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Feride Efe
0000-0001-6012-9680
Türkiye

Zehranur Yuksekdag
0000-0002-0381-5876
Türkiye

Berat Çınar Acar ^*
0000-0003-4662-0865
Türkiye

Yayımlanma Tarihi

25 Kasım 2022

Gönderilme Tarihi

3 Haziran 2022

Kabul Tarihi

4 Ağustos 2022

Yayımlandığı Sayı

Yıl 2022 Cilt: 3 Sayı: 2

IZ

https://izlik.org/JA69SG74KP

Kaynak Göster

RIS / Bibtex

APA

Efe, F., Yuksekdag, Z., & Çınar Acar, B. (2022). Lactobacillus Cinsi Bakteriler Tarafından Üretilen Biyosürfektanların Anti-Biyofilm ve Anti-Mikrobiyal Aktivitelerinin Belirlenmesi. Gazi Üniversitesi Fen Fakültesi Dergisi, 3(2), 102-115. https://izlik.org/JA69SG74KP

AMA

1.Efe F, Yuksekdag Z, Çınar Acar B. Lactobacillus Cinsi Bakteriler Tarafından Üretilen Biyosürfektanların Anti-Biyofilm ve Anti-Mikrobiyal Aktivitelerinin Belirlenmesi. GÜFFD. 2022;3(2):102-115. https://izlik.org/JA69SG74KP

Chicago

Efe, Feride, Zehranur Yuksekdag, ve Berat Çınar Acar. 2022. “Lactobacillus Cinsi Bakteriler Tarafından Üretilen Biyosürfektanların Anti-Biyofilm ve Anti-Mikrobiyal Aktivitelerinin Belirlenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi 3 (2): 102-15. https://izlik.org/JA69SG74KP.

EndNote

Efe F, Yuksekdag Z, Çınar Acar B (01 Kasım 2022) Lactobacillus Cinsi Bakteriler Tarafından Üretilen Biyosürfektanların Anti-Biyofilm ve Anti-Mikrobiyal Aktivitelerinin Belirlenmesi. Gazi Üniversitesi Fen Fakültesi Dergisi 3 2 102–115.

IEEE

[1]F. Efe, Z. Yuksekdag, ve B. Çınar Acar, “Lactobacillus Cinsi Bakteriler Tarafından Üretilen Biyosürfektanların Anti-Biyofilm ve Anti-Mikrobiyal Aktivitelerinin Belirlenmesi”, GÜFFD, c. 3, sy 2, ss. 102–115, Kas. 2022, [çevrimiçi]. Erişim adresi: https://izlik.org/JA69SG74KP

ISNAD

Efe, Feride - Yuksekdag, Zehranur - Çınar Acar, Berat. “Lactobacillus Cinsi Bakteriler Tarafından Üretilen Biyosürfektanların Anti-Biyofilm ve Anti-Mikrobiyal Aktivitelerinin Belirlenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi 3/2 (01 Kasım 2022): 102-115. https://izlik.org/JA69SG74KP.

JAMA

1.Efe F, Yuksekdag Z, Çınar Acar B. Lactobacillus Cinsi Bakteriler Tarafından Üretilen Biyosürfektanların Anti-Biyofilm ve Anti-Mikrobiyal Aktivitelerinin Belirlenmesi. GÜFFD. 2022;3:102–115.

MLA

Efe, Feride, vd. “Lactobacillus Cinsi Bakteriler Tarafından Üretilen Biyosürfektanların Anti-Biyofilm ve Anti-Mikrobiyal Aktivitelerinin Belirlenmesi”. Gazi Üniversitesi Fen Fakültesi Dergisi, c. 3, sy 2, Kasım 2022, ss. 102-15, https://izlik.org/JA69SG74KP.

Vancouver

1.Feride Efe, Zehranur Yuksekdag, Berat Çınar Acar. Lactobacillus Cinsi Bakteriler Tarafından Üretilen Biyosürfektanların Anti-Biyofilm ve Anti-Mikrobiyal Aktivitelerinin Belirlenmesi. GÜFFD [Internet]. 01 Kasım 2022;3(2):102-15. Erişim adresi: https://izlik.org/JA69SG74KP