Biyouyumlu agaroz-β-siklodekstrin hidrojellerinin karakterizasyonu ve ilaç salım özelliklerinin araştırılması

Öz

Hidrojeller, yüksek su tutma kapasitesi ve hidrofilik özellikler sergileyen, gözenekli, üç boyutlu, çapraz bağlı polimer ağlarından oluşan yumuşak malzemeler olarak tanımlanır. Hidrojellerin kimyasal, fiziksel, mekanik ve biyolojik özellikleri, bunların çok sayıda alanda kullanılmasına olanak sağlamaktadır. Son yıllarda, özellikle ilaç salım teknolojilerinde hidrojellerin artan tercihi, bu alana olan ilgiyi önemli ölçüde artırmıştır. Mevcut çalışmada, farklı oranlarda β-siklodekstrin (βCD) kullanılarak agaroz-β-siklodekstrin (AGβCD) içeren hidrojeller halka açılma polimerizasyon tekniği ile hazırlandı. Daha sonra değişen oranda βCD içeriğinin hidrojelin ilaç salım eğilimi üzerine etkisi araştırıldı. Elde edilen hidrojellerin yapısal, morfolojik ve termal karakterizasyonları sırasıyla FTIR, SEM ve TGA kullanılarak aydınlatıldı. Daha sonra bunların şişme oranları ve su içerikleri gravimetrik yöntemle belirlendi. Hidrojellerin in vitro ilaç salım davranışları ve biyouyumlulukları mikroplaka okuyucu kullanılarak gerçekleştirildi. Sonuçlar yapıdaki βCD oranının azalmasıyla hidrojellerin şişme oranının, su içeriklerinin ve ilaç yükleme yüzdesinin arttığını gösterdi. Ayrıca, hidrofilik 5-florourasilin daha yüksek salım eğiliminin, hidrojellerdeki βCD miktarındaki artışla desteklendiğini göstermişlerdir. Hücre kültürü çalışmaları hidrojellerin toksik olmadığını ortaya koydu. Sonuç olarak, hazırlanan biyouyumlu AGβCD hidrojellerinin ilaç salım alanında kullanım potansiyeline sahip olduğu söylenebilir.

Anahtar Kelimeler

• Agaroz
• β-siklodekstrin
• 5-florourasil
• hidrojel
• ilaç salımı

Characterization of biocompatible agarose-β-cyclodextrin hydrogels and investigation of their drug release properties

Öz

Hydrogels are defined as soft materials composed of porous, three-dimensional, cross-linked polymer networks exhibiting a large water-holding capacity and hydrophilic characteristics. The chemical, physical, mechanical, and biological characteristics of hydrogels enable them to be employed in numerous fields. Recently, the growing preference for hydrogels, particularly in drug delivery technologies, has significantly enhanced interest in this field. In the current study, hydrogels containing agarose-β-cyclodextrin (AGβCD) were prepared using different ratios of β-cyclodextrin (βCD) via the ring-opening polymerization technique. The potency of various βCD contents on the drug release trend of the hydrogel was then explored. The structural, morphological, and thermal characterizations of the obtained hydrogels were elucidated using FTIR, SEM, and TGA, respectively. Then, their swelling ratios and moisture contents were determined by the gravimetric method. In vitro drug release behaviour and biocompatibility of these materials were realized using a microplate reader. The results signified that the swelling ratio, moisture content, and drug loading percentage of the hydrogels increased as the proportion of βCD in the structure decreased. Moreover, they displayed that the higher release tendency of the hydrophilic 5-fluorouracil was promoted by an increase in the amount of βCD in the hydrogels. Cell culture studies revealed that the hydrogels were non-toxic. As a result, it can be stated that the prepared biocompatible AGβCD hydrogels have the potential for utilization in the field of drug release.

Anahtar Kelimeler

• Agarose
• β-cyclodextrin
• 5-fluorouracil
• hydrogel
• drug release

Kaynakça

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