The Voltammetric Determination of Epinephrine on an Au Electrode Modified with Electropolymerized 3,5-Diamino-1,2,4- Triazole Film

Abstract

A gold electrode (Au) was modified with 3,5-diamino-1,2,4-triazole (35DT). The electrochemical behavior of epinephrine (EP) was evaluated on the modified electrode (35DT-Au) by the differential pulse voltammetry (DPV) and cyclic voltammetry (CV) techniques. For the oxidation of EP, the 35DT-Au modified electrode surface showed an good electrocatalytic activity. The modified electrode was used to determine EP by using DPV technique. Under the optimum conditions, the calibration curve for EP was obtained in the ranges of 0.9 to 32.31 μM and 32.31 to 1050 μM and the detection limits (LOD) was found as 0.39 and 12.33 μM, respectively. For the oxidation process of EP, the 35DT-Au modified electrode exhibited a good repeatability, reproducibility, stability and sensitivity. Finally, the determination of EP in commercial injectable adrenaline samples were successfully applied.

Keywords

• Epinephrine
• Coating
• Electrochemical
• behaviour
• Voltammetric
• determination
• Drug

Elektropolimerize 3,5-Diamino-1,2,4-Triazol Film ile Modifiye Edilmiş Altın Elektrot Yüzeyinde Epinefrinin Voltametrik Tayini ve Elektrokimyasal Davranışı

Öz

Bu çalışmada, altın elektrot (Au) yüzeyi 3,5-diamino-1,2,4-triazol (35DT) molekülü ile elektrokimyasal yöntemle modifiye edilmiştir. Epinefrinin (EP) modifiye elektrot (35DT-Au) yüzeyindeki elektrokimyasal davranışı diferansiyel puls voltametrisi (DPV) ve dönüşümlü voltametri (CV) teknikleriyle incelenmiştir. EP'nin yükseltgenme işleminde 35DT-Au modifiye elektrot yüzeyi iyi bir elektrokatalitik aktivite göstermiştir. EP tayini, 35DT-Au modifiye elektrot yüzeyinde DPV tekniği kullanılarak gerçekleştirilmiştir. Optimum şartlar altında, 35DT-Au modifiye elektrot ile EP’ye ait doğrusal çalışma aralığı 0,9-32,31 μM ve 32,31-1050 μM olmak üzere iki farklı derişim aralığı olarak belirlenmiştir. Bu iki derişim aralığı için gözlenebilme sınırları (LOD) sırasıyla 0,39 ve 12,33 μM olarak bulunmuştur. EP tayini için, 35DT-Au elektrot iyi bir tekrarlanabilirlik, kararlılık ve hassasiyet göstermiştir. Ayrıca, gerçek numune olarak ilaç numunei seçilmiş ve ilaçta EP tayini 35DT-Au elektrot ile başarıyla gerçekleştirilmiştir.

Anahtar Kelimeler

• Epinefrin
• Kaplama
• Elektrokimyasal Davranış
• Voltametrik tayin
• ilaç

Kaynakça

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