Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability

Esra Bulut Atalay

doi:10.17714/gumusfenbil.1353355

TR EN

İzositrat dehidrojenaz 1 ve izositrat dehidrojenaz 2 genlerinin gen ekspresyon değişiklikleri karsinojenezi ve hayatta kalma olasılığını etkiler

Öz

İzositrat dehidrojenaz (IDH), hücre metabolizmasının düzenlenmesinde önemli bir metabolik enzimdir. IDH geni, IDH1, IDH2 ve IDH3 olmak üzere üç protein izoformunu kodlar ve izoformların ekspresyon seviyesi, insan kanser türlerinde değişiklik gösterir. IDH'nin gen ekspresyon seviyesinin incelenmesi, kanser metabolizması alanında kullanılacak yeni bir hedef bulmaya yardımcı olabilecek terapötik bir avantajdır. Bu çalışmanın amacı, biyoinformatik araçlar kullanarak on yaygın insan kanserinde IDH1 ve IDH2 izoformlarının gen ekspresyon seviyesini araştırmaktır. Ek olarak, IDH1 ve IDH2’nin gen ekspresyon seviyesindeki değişikliklerin karsinogenez ve hayatta kalma olasılığı üzerindeki etkisi TCGA veri tabanında depolanan halka açık veriler üzerinde incelenmiştir. Elde edilen sonuçlar, IDH izoformlarının ekspresyonunun dokuya özgü farklılıklar ve heterojen özellik sergilediğini gösterdi. IDH1 ekspresyonu özofagus ve akciğer skuamöz hücreli karsinom ile akciğer ve mide adenokarsinomu tümörlerinde arttı. Mesane ürotelyal, meme ürotelyal ve akciğer skuamöz hücreli karsinomu ve kolon ve akciğer adenokarsinomu, IDH2 ekspresyonunda önemli bir artış sergiledi. IDH izoformlarının ekspresyonu ile çeşitli kanser türlerinin ilerlemesi arasında doğrudan bir ilişki olduğu bulundu. Yüksek IDH1 ekspresyonu, özofagus karsinomu, akciğer ve mide adenokarsinomunda hayatta kalma olasılığının azalmasına yol açtı. Yüksek IDH2 ekspresyon seviyesi, mesane ürotelyal, meme ürotelyal ve akciğer skuamöz hücreli karsinom ve kolon adenokarsinomunda hayatta kalma olasılığının azalmasına yol açtı. Sonuç olarak, tüm veriler IDH1'in özofagus karsinomu, akciğer ve mide adenokarsinomu için ve IDH2'nin mesane ürotelyal, meme ürotelyal ve akciğer skuamöz hücreli karsinom ve kolon adenokarsinomu için bir biyobelirteç olabileceğini gösterdi.

Anahtar Kelimeler

Biyoinformatik, Karsinogenez, İzositrat dehidrojenaz 1, İzositrat dehidrojenaz 2

Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability

Öz

Isocitrate dehydrogenase (IDH) is an essential metabolic enzyme in the regulation of cellular metabolism. IDH gene encodes three protein isoforms, IDH1, IDH2, and IDH3, and the expression level of isoforms is altered in human cancer types. Examining the gene expression level of IDH is a therapeutic advantage that could help find a new target to use in cancer metabolism. The present study aimed to explore the gene expression level of IDH1 and IDH2 isoforms in the ten common human cancers using bioinformatic tools. In addition, the effect of gene expression changes on IDH1 and IDH2 on carcinogenesis and survival probability was examined in publicly available data deposited in the TCGA database. The results showed that the expression of IDH isoforms showed tissue-specific differences. IDH1 expression increased in esophageal and lung squamous cell carcinoma and lung and stomach adenocarcinoma tumors. Bladder urothelial, breast urothelial, and lung squamous cell carcinoma, colon, and lung adenocarcinoma displayed a significant upregulation of IDH2 expression. There was a direct relationship between the expression of IDH isoforms and the progression of various cancer types. High IDH1 expression led to decreased survival probability in esophageal carcinoma, lung, and stomach adenocarcinoma. Elevated IDH2 expression level led to decreased survival probability in bladder urothelial, breast urothelial, and lung squamous cell carcinoma and colon adenocarcinoma. In conclusion, all data showed that IDH1 could be a biomarker for esophageal carcinoma, lung and stomach adenocarcinoma, and IDH2 for bladder urothelial, breast urothelial, and lung squamous cell carcinoma, and colon adenocarcinoma.

Anahtar Kelimeler

Bioinformatic, Carcinogenesis, Isocitrate dehydrogenase 1, Isocitrate dehydrogenase 2

Kaynakça

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Ayrıntılar

Birincil Dil

İngilizce

Konular

İstatistiksel ve Nicel Genetik, Kanser Biyolojisi

Bölüm

Araştırma Makalesi

Yazarlar

Esra Bulut Atalay ^*
0000-0002-1615-0535
Türkiye

Yayımlanma Tarihi

15 Mart 2024

Gönderilme Tarihi

31 Ağustos 2023

Kabul Tarihi

3 Ocak 2024

Yayımlandığı Sayı

Yıl 2024 Cilt: 14 Sayı: 1

DOI

https://doi.org/10.17714/gumusfenbil.1353355

IZ

https://izlik.org/JA79ZH27YG

APA

Bulut Atalay, E. (2024). Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 14(1), 370-378. https://doi.org/10.17714/gumusfenbil.1353355

AMA

1.Bulut Atalay E. Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 2024;14(1):370-378. doi:10.17714/gumusfenbil.1353355

Chicago

Bulut Atalay, Esra. 2024. “Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability”. Gümüşhane Üniversitesi Fen Bilimleri Dergisi 14 (1): 370-78. https://doi.org/10.17714/gumusfenbil.1353355.

EndNote

Bulut Atalay E (01 Mart 2024) Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability. Gümüşhane Üniversitesi Fen Bilimleri Dergisi 14 1 370–378.

IEEE

[1]E. Bulut Atalay, “Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability”, Gümüşhane Üniversitesi Fen Bilimleri Dergisi, c. 14, sy 1, ss. 370–378, Mar. 2024, doi: 10.17714/gumusfenbil.1353355.

ISNAD

Bulut Atalay, Esra. “Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability”. Gümüşhane Üniversitesi Fen Bilimleri Dergisi 14/1 (01 Mart 2024): 370-378. https://doi.org/10.17714/gumusfenbil.1353355.

JAMA

1.Bulut Atalay E. Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 2024;14:370–378.

MLA

Bulut Atalay, Esra. “Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability”. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, c. 14, sy 1, Mart 2024, ss. 370-8, doi:10.17714/gumusfenbil.1353355.

Vancouver

1.Esra Bulut Atalay. Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 01 Mart 2024;14(1):370-8. doi:10.17714/gumusfenbil.1353355

e-ISSN: 2146-538X Yayın Modeli: Sürekli Yayın Atıf Dizinleri: TR Dizin , SCOPUS

İzositrat dehidrojenaz 1 ve izositrat dehidrojenaz 2 genlerinin gen ekspresyon değişiklikleri karsinojenezi ve hayatta kalma olasılığını etkiler

Öz

Anahtar Kelimeler

Gene expression changes of isocitrate dehydrogenase 1 and isocitrate dehydrogenase 2 affect carcinogenesis and survival probability

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster