İki Convolvulus L. Türünün In-Vitro Nitrik Oksit Süpürme ve Anti-Tirozinaz Aktivitesi

Yıl 2019, , 383 - 387, 15.07.2019

Cennet Özay

https://doi.org/10.17714/gumusfenbil.404024

Öz

Convolvulus
L., Convolvulaceae familyasının bir üyesi olup, biyolojik
aktivitelerinden dolayı halk arasında tıbbi amaçlı kullanılmaktadır. Bu
çalışmanın amacı, iki Convolvulus
türünün tirozinaz aktivite üzerindeki inhibitör etkisi ve nitrik oksit (NO)
süpürme aktivite potansiyellerini belirlemektir. Şimdiye kadar yapılan
yayınlarda bu iki bitkinin anti-tirozinaz ve NO süpürme aktivite özelliklerine
ilişkin bir rapor bulunamamıştır. C. galaticus ve C. phrygius toprak üstü kısımları metanolde
ekstrakte edildikten sonra ekstraktların radikal süpürme aktivitelerinin
belirlenmesi için NO süpürme aktivite deneyi kullanıldı. Ekstraktların anti-tirozinaz aktivitesi,
96-kuyucuklu mikroplaka metodunun modifiye edilmesiyle belirlendi. En yüksek NO
süpürme ve anti-tirozinaz aktivitesi C. galaticus'ta sırasıyla % 55.20 ve % 53.16 olarak bulundu.

Anahtar Kelimeler

Convolvulus L., nitrik oksit süpürme aktivitesi, bitki ekstraktı, tirozinaz inhibisyonu

In-Vitro Nitric Oxide Scavenging and Anti-Tyrosinase Activity of Two Convolvulus L. Species

Öz

Convolvulus L. is a
member of Convolvulaceae family which have been utilized for their biological
activities in folk medicine. The aim of this study is to investigate the inhibitory
effect on tyrosinase activity of two Convolvulus species as well as their nitric oxide (NO) scavenging activity potentials.
Up to now, no reports have been found on their anti-tyrosinase
and NO scavenging activity properties in the
literature. C.
galaticus and C. phrygius aerial
parts were extracted with methanol and then NO scavenging activity assay was utilized for the assurance of
radical scavenging activity of the extracts. Anti-tyrosinase activity
of the extracts was determined using the modified 96-well microplate method.
The highest NO scavenging and anti-tyrosinase activity were found in C. galaticus as
55.20% and 53.16%, respectively.

Anahtar Kelimeler

Convolvulus L., nitric oxide scavenging activity, plant extract, tyrosinase inhibition

Kaynakça

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