Drosophila melanogaster’in farklı soylarında astaksantin’in in vivo kronik etkilerinin belirlenmesi

Yıl 2021, , 135 - 146, 15.01.2021

Handan Uysal , Hatice Çelik

https://doi.org/10.17714/gumusfenbil.754474

Öz

Rodofit olarak bilinen alglerde bulunan ve kırmızı renkli bir pigment olan astaksantin (ASTA) terpeni bir tetraterpendir. Çalışmada ASTA’nın doz-süre etkileşimine bağlı olarak olası toksik ve genotoksik etkileri araştırılmıştır. ASTA’nın toksik etkisi, Drosophila melanogaster’in Oregon-R yabanıl soyunda in vivo ömür uzunluğu testi ile belirlenmiştir. Bu amaçla ergin dişi ve erkek bireyler farklı dozlarda ASTA içeren (25, 50, 100 ve 200 ppm) besiyerinde kronik olarak beslenmiştir. Kontrol grubunda ortalama ömür uzunluğu dişilerde 49.07±1.92 gün iken erkeklerde 51.01±2.12 gündür. Bu değerler en düşük ve en yüksek ASTA uygulama gruplarında (25-200 ppm) dişilerde sırasıyla 32.06±1.50 ve 27.06±1.18 gün, erkeklerde de 32.45±1.48 ve 23.52±0.92 gün olarak bulunmuştur. ASTA’nın kontrol ve uygulama gruplarına ait sonuçlar birbiriyle karşılaştırıldığı zaman hem dişi hem de erkek popülasyonunda ortalama ömür uzunluğunun doz-süre etkileşimine bağlı olarak kısaldığı gözlenmiştir (p<0.05). ASTA terpeninin genotoksik etkisi de yine D.melanogaster’de somatik mutasyon ve rekombinasyon testi (SMART) ile belirlenmiştir. SMART sonucunda elde edilen verilere göre tüm ASTA uygulama gruplarında (50, 100, 200 ve 400 ppm) konsantrasyon artışına bağlı olarak klon indüksiyon frekansında artış gözlenmiştir. Bu sonuçlar ASTA’nın somatik mutasyonları uyardığını göstermektedir.

Anahtar Kelimeler

Genotoksik etki, Karotenoid, Ömür uzunluğu, Somatik mutasyon

Destekleyen Kurum

Atatürk Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi

Proje Numarası

FHD–2019-7000

Teşekkür

Atatürk Üniversitesi Bilimsel Araştırma Projeleri Yönetim Birimi Başkanlığı'na destekleri için teşekkür ederiz.

Determination of ın vivo chronic effects of astaxanthin in different strains of drosophila melanogaster

Öz

Astaxanthine (ASTA) terpene, a red pigment found in algae known as rhodopite, is a tetraterpene. In this study, possible toxic and genotoxic effects of the ASTA were investigated depending on the dose-time interaction. The toxic effect of the ASTA was determined by in vivo longevity test in Oregon-R wild strain of Drosophila melanogaster. For this purpose, adult female and male individuals were chronically fed on media containing different doses of the ASTA (25, 50, 100 and 200 ppm). While the average life span in the control group is 49.07±1.92 days in females, it is 51.01±2.12 days in males. These values are 32.06±1.50 and 27.06±1.18 days in females in the lowest and highest ASTA application groups (25-200 ppm), 32.45±1.48 and 23.52±0.92 in males, respectively. When the results of the control and application groups of the ASTA terpene were compared, it was observed that the average life span decreased in both female and male populations depending on the dose-time interaction (p<0.05). The genotoxic effect of the ASTA terpene was also determined by the somatic mutation and recombination test (SMART) in D.melanogaster. According to the data obtained from the SMART result, an increase in clone induction frequency was observed in all the ASTA application groups (50, 100, 200 and 400 ppm) due to concentration increase. These results show that ASTA stimulates somatic mutations.

Anahtar Kelimeler

Genotoxic effects, Carotenoid, Longevity, Somatic mutation

Proje Numarası

FHD–2019-7000

Kaynakça

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