Demir (III) oksit (Fe2O3) nanopartiküllerinin genotoksisitesinin Drosophila hemositlerinde KOMET yöntemi ile araştırılması

Burcin Yalcin; Merve Güneş; İbrahim Hakkı Ciğerci; Bülent Kaya

doi:10.17714/gumusfenbil.821244

EN TR

Investigation of genotoxicity of iron (III) oxide (Fe2O3) nanoparticles in Drosophila hemocytes by COMET method

Abstract

The use of nanoparticles (NPs) has become widespread due to the increasing diversification of products from NP and the benefit of these products for economic, environmental and human health. However, this overutilize has also led to some concerns. NPs can show different toxic effects depending on their chemical composition, structure, particle size, surface area and shape. Their shape and size are important determinants in the cellular uptake and potential toxicity of NPs. Iron (III) oxide (Fe2O3) NPs are used in many biomedical and bioengineering fields such as magnetic resonance imaging, drug delivery, detoxification of biological fluids. In this study, COMET (alkaline single cell gel electrophoresis) analysis was performed with Drosophila hemocytes to determine the relationship between the genotoxicity of Fe2O3 NPs and particle size and shape. According to the result of COMET, no statistically significant difference was found in terms of genotoxicity when Fe2O3 NPs with of <50 nm and sphere form and Fe2O3 NPs with of <100 nm and rod form were compared. However, in both Fe2O3 NP treatments, it was determined that only 1 mM dose (between 1, 2 and 5 mM) was induced statistically significant level of genoxicity compared to the control group distilled water.

Keywords

Iron oxide , Drosophila , Genotoxicity , COMET , Nanoparticle

Demir (III) oksit (Fe2O3) nanopartiküllerinin genotoksisitesinin Drosophila hemositlerinde KOMET yöntemi ile araştırılması

Öz

Nanopartikül (NP) kaynaklı ürünlerin giderek çeşitlenmesi ve bu ürünlerin ekonomik, çevresel ve insan sağlığı yararına fayda sağlaması nedeni ile NP’lerin kullanımı yaygınlaşmıştır. Ancak bu yoğun kullanım beraberinde bazı endişelerin oluşmasına da yol açmıştır. NP’ler kimyasal bileşimlerine, yapılarına, partikül büyüklüklerine, yüzey alanlarına ve şekillerine göre farklı toksik etkiler gösterebilmektedir. Şekilleri ve boyutları NP’lerin hücresel alımları ve potansiyel toksisitesinde önemli belirleyicilerdir. Demir (III) oksit (Fe2O3) NP’leri, manyetik rezonans görüntülemede, ilaç dağıtımında, biyolojik sıvıların detoksifikasyonu gibi birçok biyomedikal ve biyomühendislik alanlarında kullanıma sahiptir. Bu çalışmada Fe2O3 NP’lerinin genotoksisitesinin partikül boyutu ve şekli ile ilişkisini belirlemek amacıyla Drosophila hemositleri ile KOMET (alkali tek hücre jel elektroforez) analizi gerçekleştirilmiştir. KOMET sonucuna göre, <50 nm boyutlu ve küre forma sahip Fe2O3 NP’leri ile <100 nm boyutlu ve çubuk forma sahip Fe2O3 NP’lerinin genotoksik etkileri karşılaştırıldığında istatistiksel olarak anlamlı bir farklılık bulunmamıştır. Ancak her iki Fe2O3 NP uygulamasında da çalışılan 3 farklı dozdan (1, 2 ve 5 mM) sadece 1 mM’lık uygulamada kontrol grubu distile suya göre istatistiksel olarak anlamlı düzeyde genoksisitenin indüklendiği belirlenmiştir.

Anahtar Kelimeler

Demir oksit , Drosophila , Genotoksisite , KOMET , Nanopartikül

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Burcin Yalcin
0000-0002-9694-5839
Türkiye

Merve Güneş
0000-0003-3278-0542
Türkiye

İbrahim Hakkı Ciğerci
0000-0002-3626-7730
Türkiye

Bülent Kaya ^*
0000-0002-0491-9781
Türkiye

Yayımlanma Tarihi

15 Temmuz 2021

Gönderilme Tarihi

10 Kasım 2020

Kabul Tarihi

7 Nisan 2021

Yayımlandığı Sayı

Yıl 2021 Cilt: 11 Sayı: 3

DOI

https://doi.org/10.17714/gumusfenbil.821244

IZ

https://izlik.org/JA79XD43WY

APA

Yalcin, B., Güneş, M., Ciğerci, İ. H., & Kaya, B. (2021). Demir (III) oksit (Fe2O3) nanopartiküllerinin genotoksisitesinin Drosophila hemositlerinde KOMET yöntemi ile araştırılması. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 11(3), 643-652. https://doi.org/10.17714/gumusfenbil.821244

e-ISSN: 2146-538X Yayın Modeli: Sürekli Yayın Atıf Dizinleri: TR Dizin , SCOPUS

Investigation of genotoxicity of iron (III) oxide (Fe2O3) nanoparticles in Drosophila hemocytes by COMET method

Abstract

Keywords

Demir (III) oksit (Fe2O3) nanopartiküllerinin genotoksisitesinin Drosophila hemositlerinde KOMET yöntemi ile araştırılması

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

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