Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti

Mehmet Ali Temiz

doi:10.17714/gumusfenbil.935653

EN TR

Determination of phenolic component, antioxidant capacity, and antidiabetic properties of Sisymbrium altissimum leaves

Öz

Diabetes Mellitus is characterized by high blood glucose (hyperglycemia) and is among the most suffering diseases all over the world. Hyperglycemia plays a role in the pathogenesis of many diseases by triggering the mechanisms of oxidative stress. Phytotherapy is one of the alternative ways to cope with these diseases. Medicinal, aromatic and wild edible plants have nutraceutically protective effects against various diseases as well as nutritional and dietetic value due to the phytochemicals. Sisymbrium species are among the wild edible plants and traditionally used to treatment of various diseases. In this study, it was aimed to investigate the phenolic components of Sisymbrium altissimum leaves as well as their antioxidant capacity and antidiabetic properties. For this purpose, phenolic component was determined in S. altissimum leaf extract (SAE) by high performance liquid chromatography (HPLC). In addition, total phenolic and total flavonoid content measurement, 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis- (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) free radical scavenging activity analyzes were performed to determine the antioxidant capacity. The antidiabetic effect of the leaves was measured by α-amylase and α-glucosidase inhibition activity assay. In the results obtained, it was determined that SAE showed a significant antioxidant activity. It was determined that the most abundant phenolic component in leaves was rutin, also quercetin and protocatechuic acid were the other dominant phenolic components in leaves. On the other hand, it was found that the α-amylase and α-glucosidase inhibition activities of the leaves were lower compared to acarbose. As a result, it can be concluded that S. altissimum can strengthen the antioxidant status with its important phenolic compounds and also prevent nutraceutically postprandial hyperglycemia due to its partial α-amylase and α-glucosidase inhibition activities.

Anahtar Kelimeler

Antioxidant activity, α-amylase, α-glucosidase, Phenolic compound, Sisymbrium altissimum

Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti

Öz

Diabetes Mellitus (Şeker hastalığı), yüksek kan glukozu (hiperglisemi) ile karakterize edilen ve tüm dünyada en çok muzdarip olunan hastalıklar arasındadır. Hiperglisemi oksidatif stresin oluşum mekanizmalarını tetikleyerek pek çok hastalığın patogenezinde rol oynar. Fitoterapi bu hastalıklar ile başa çıkmada alternatif yollardan biridir. Tıbbi, aromatik ve yabani yenilebilir bitkiler içerdikleri fitokimyasallar sayesinde nutrasötik olarak çeşitli hastalıklara karşı koruyucu etki göstermelerinin yanı sıra besleyici ve diyetetik özelliklere sahiptirler. Sisymbrium türleri yabani yenilebilir bitkiler arasında olup geleneksel olarak çeşitli hastalıkların tedavisinde kullanılmaktadır. Bu çalışmada, Sisymbrium altissimum yapraklarının fenolik bileşiminin yanı sıra antioksidan kapasitesinin ve antidiyabetik özelliklerinin araştırılması amaçlanmıştır. Bu maksatla S. altissimum yaprak ekstraktında (SAE) yüksek performanslı sıvı kromatografisi (HPLC) ile fenolik bileşen tespiti yapıldı. Ayrıca, antioksidan kapasitesini belirlemek amacıyla toplam fenolik ve toplam flavonoid madde tayini ile 2,2-difenil-1-pikrilhidrazil (DPPH) ve 2,2′-Azino-bis (3-etilbenzotiazolin-6-sülfonik asit) (ABTS) serbest radikal temizleme aktivite analizleri gerçekleştirildi. Yaprakların antidiyabetik etkisi, α-amilaz ve α-glukozidaz inhibisyon aktivite tayini ile ölçüldü. Elde edilen sonuçlarda, SAE’nin önemli düzeyde antioksidan aktivite gösterdiği belirlendi. Yapraklarda en çok bulunan fenolik bileşenin rutin olduğu ayrıca, kuersetin ve protokateşuik asidin ise yapraklardaki diğer baskın fenolik bileşenler olduğu saptandı. Diğer taraftan yaprakların α-amilaz ve α-glukozidaz inhibisyon aktivitelerinin akarboz ile karşılaştırıldığında daha düşük olduğu tespit edildi. Sonuç olarak, S. altissimum’um önemli fenolik bileşen içeriğiyle antioksidan durumu güçlendirebileceği ve ayrıca kısmi α-amilaz ve α-glukozidaz inhibisyon aktiviteleri sayesinde nutrasötik olarak yemek sonrası hiperglisemiyi önleyebileceği sonucuna varılabilir.

Anahtar Kelimeler

Antioksidan aktivite, α-amilaz, α-glukozidaz, Fenolik bileşik, Sisymbrium altissimum

Kaynakça

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Ayrıntılar

Birincil Dil

Türkçe

Konular

-

Bölüm

Araştırma Makalesi

Yazarlar

Mehmet Ali Temiz ^*
0000-0002-4680-3023
Türkiye

Yayımlanma Tarihi

15 Ocak 2022

Gönderilme Tarihi

10 Mayıs 2021

Kabul Tarihi

27 Ekim 2021

Yayımlandığı Sayı

Yıl 2022 Cilt: 12 Sayı: 1

DOI

https://doi.org/10.17714/gumusfenbil.935653

IZ

https://izlik.org/JA26XA87XN

APA

Temiz, M. A. (2022). Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, 12(1), 68-77. https://doi.org/10.17714/gumusfenbil.935653

AMA

1.Temiz MA. Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 2022;12(1):68-77. doi:10.17714/gumusfenbil.935653

Chicago

Temiz, Mehmet Ali. 2022. “Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti”. Gümüşhane Üniversitesi Fen Bilimleri Dergisi 12 (1): 68-77. https://doi.org/10.17714/gumusfenbil.935653.

EndNote

Temiz MA (01 Ocak 2022) Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti. Gümüşhane Üniversitesi Fen Bilimleri Dergisi 12 1 68–77.

IEEE

[1]M. A. Temiz, “Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti”, Gümüşhane Üniversitesi Fen Bilimleri Dergisi, c. 12, sy 1, ss. 68–77, Oca. 2022, doi: 10.17714/gumusfenbil.935653.

ISNAD

Temiz, Mehmet Ali. “Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti”. Gümüşhane Üniversitesi Fen Bilimleri Dergisi 12/1 (01 Ocak 2022): 68-77. https://doi.org/10.17714/gumusfenbil.935653.

JAMA

1.Temiz MA. Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 2022;12:68–77.

MLA

Temiz, Mehmet Ali. “Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti”. Gümüşhane Üniversitesi Fen Bilimleri Dergisi, c. 12, sy 1, Ocak 2022, ss. 68-77, doi:10.17714/gumusfenbil.935653.

Vancouver

1.Mehmet Ali Temiz. Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti. Gümüşhane Üniversitesi Fen Bilimleri Dergisi. 01 Ocak 2022;12(1):68-77. doi:10.17714/gumusfenbil.935653

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e-ISSN: 2146-538X Yayın Modeli: Sürekli Yayın Atıf Dizinleri: TR Dizin , SCOPUS

Determination of phenolic component, antioxidant capacity, and antidiabetic properties of Sisymbrium altissimum leaves

Öz

Anahtar Kelimeler

Sisymbrium altissimum yapraklarının fenolik bileşen, antioksidan kapasite ve antidiyabetik özelliklerinin tespiti

Öz

Anahtar Kelimeler

Kaynakça

Ayrıntılar

Birincil Dil

Konular

Bölüm

Yazarlar

Yayımlanma Tarihi

Gönderilme Tarihi

Kabul Tarihi

Yayımlandığı Sayı

DOI

IZ

Kaynak Göster

Cited By

The effect of maturity, storage duration, and conditions on service tree (Sorbus domestica L.) fruit vinegars: physicochemical, phytochemical, and anti-diabetic properties

Comparative optimization of traditional and ultrasound-assisted extraction methods for phenolic compounds from Sorbus domestica leaves: antioxidant and antidiabetic properties